Proteome-pI 2.0 - Proteome Isoelectric Point Database

Treponema maltophilum ATCC 51939

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Spirochaetales; Treponemataceae; Treponema; Treponema maltophilum

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 2287 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|S3K5N5|S3K5N5_TREMA Uncharacterized protein OS=Treponema maltophilum ATCC 51939 OX=1125699 GN=HMPREF9194_00282 PE=3 SV=1

MM1 pKa = 7.64AKK3 pKa = 9.93YY4 pKa = 10.01VCTVCGYY11 pKa = 10.27EE12 pKa = 3.99YY13 pKa = 10.86DD14 pKa = 4.14PAVGDD19 pKa = 4.06PDD21 pKa = 4.9GSIAPGTAFEE31 pKa = 5.97DD32 pKa = 4.28IPDD35 pKa = 3.7DD36 pKa = 3.88WVCPVCGVGKK46 pKa = 10.34DD47 pKa = 3.41SFSKK51 pKa = 11.16VEE53 pKa = 3.78

Molecular weight: 5.61 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|S3K4J1|S3K4J1_TREMA Uncharacterized protein OS=Treponema maltophilum ATCC 51939 OX=1125699 GN=HMPREF9194_02261 PE=3 SV=1

MM1 pKa = 6.51EE2 pKa = 4.25QCRR5 pKa = 11.84SAHH8 pKa = 6.37SIPLHH13 pKa = 5.97DD14 pKa = 4.67HH15 pKa = 6.75ASFAQQHH22 pKa = 4.72VTQIAQNTARR32 pKa = 11.84MVDD35 pKa = 3.53KK36 pKa = 10.76AAEE39 pKa = 3.82RR40 pKa = 11.84CIIVRR45 pKa = 11.84SFVRR49 pKa = 11.84SFVRR53 pKa = 11.84SFVRR57 pKa = 11.84SFVRR61 pKa = 11.84SFVRR65 pKa = 11.84SCSVNYY71 pKa = 7.86TQNTKK76 pKa = 8.6SVKK79 pKa = 9.56QLNDD83 pKa = 3.54SGCTNPLQKK92 pKa = 10.44KK93 pKa = 9.13AASKK97 pKa = 10.78HH98 pKa = 5.18SLADD102 pKa = 3.25AALRR106 pKa = 11.84LCRR109 pKa = 11.84IFFAAEE115 pKa = 3.52LRR117 pKa = 11.84KK118 pKa = 10.32NCTSLYY124 pKa = 9.78NSFIAFCAPCKK135 pKa = 10.5GRR137 pKa = 11.84IRR139 pKa = 11.84KK140 pKa = 7.65PFPASLPEE148 pKa = 3.84HH149 pKa = 6.58TIFSQYY155 pKa = 9.54TIRR158 pKa = 11.84TKK160 pKa = 10.62DD161 pKa = 2.72ISSFVRR167 pKa = 11.84FFILSQKK174 pKa = 9.29EE175 pKa = 3.81VNRR178 pKa = 11.84TQKK181 pKa = 10.27HH182 pKa = 3.85RR183 pKa = 3.63

Molecular weight: 20.97 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
2287	0	2287	775539	29	2297	339.1	37.63

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
9.388 ± 0.058	1.394 ± 0.019	5.308 ± 0.042	6.308 ± 0.061	5.015 ± 0.047	6.916 ± 0.047	1.56 ± 0.02	6.836 ± 0.054	6.825 ± 0.044	9.474 ± 0.047

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.304 ± 0.026	4.131 ± 0.032	3.836 ± 0.028	2.934 ± 0.029	4.784 ± 0.046	6.554 ± 0.044	5.384 ± 0.045	6.573 ± 0.038	0.946 ± 0.016	3.532 ± 0.031

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski