Streptococcus phage Javan53

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AHB1|A0A4D6AHB1_9CAUD Uncharacterized protein OS=Streptococcus phage Javan53 OX=2548242 GN=Javan53_0032 PE=4 SV=1
MM1 pKa = 7.54IDD3 pKa = 3.57YY4 pKa = 10.95YY5 pKa = 11.39SLYY8 pKa = 10.5KK9 pKa = 10.72NSLEE13 pKa = 4.46AIGYY17 pKa = 8.76HH18 pKa = 6.13NLDD21 pKa = 3.35TGVDD25 pKa = 3.8LLQYY29 pKa = 10.73LIATEE34 pKa = 4.03EE35 pKa = 4.17GKK37 pKa = 10.46RR38 pKa = 11.84IYY40 pKa = 10.2SQYY43 pKa = 10.16PYY45 pKa = 10.72RR46 pKa = 11.84EE47 pKa = 3.85VEE49 pKa = 4.1EE50 pKa = 4.69IIFYY54 pKa = 9.89TLDD57 pKa = 3.54CLIQQDD63 pKa = 4.56LVTATEE69 pKa = 4.12MPRR72 pKa = 11.84LDD74 pKa = 3.31RR75 pKa = 11.84RR76 pKa = 11.84IYY78 pKa = 10.22TIDD81 pKa = 3.29GLTPKK86 pKa = 10.51GMYY89 pKa = 9.59FLGYY93 pKa = 8.94IGQVEE98 pKa = 4.52TEE100 pKa = 4.16VIEE103 pKa = 4.03WLNEE107 pKa = 3.72FGVSQNPEE115 pKa = 4.02SIYY118 pKa = 10.78NALRR122 pKa = 11.84YY123 pKa = 9.48IIYY126 pKa = 10.55

Molecular weight: 14.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AH12|A0A4D6AH12_9CAUD DNA replication protein OS=Streptococcus phage Javan53 OX=2548242 GN=Javan53_0024 PE=4 SV=1
MM1 pKa = 7.41CRR3 pKa = 11.84VLRR6 pKa = 11.84VNRR9 pKa = 11.84STYY12 pKa = 11.05YY13 pKa = 10.82KK14 pKa = 10.17FLKK17 pKa = 10.12HH18 pKa = 6.23KK19 pKa = 9.91PSKK22 pKa = 10.33RR23 pKa = 11.84DD24 pKa = 3.18LDD26 pKa = 3.56NQIYY30 pKa = 10.06RR31 pKa = 11.84KK32 pKa = 9.69QILEE36 pKa = 4.29IYY38 pKa = 8.72TKK40 pKa = 10.72ANKK43 pKa = 9.95RR44 pKa = 11.84LGAKK48 pKa = 9.35SIKK51 pKa = 10.5VILQRR56 pKa = 11.84DD57 pKa = 3.93YY58 pKa = 10.67DD59 pKa = 4.06TKK61 pKa = 10.39ISEE64 pKa = 3.86GRR66 pKa = 11.84IYY68 pKa = 11.17RR69 pKa = 11.84LMKK72 pKa = 10.86NMALPKK78 pKa = 9.66MATIKK83 pKa = 10.68PKK85 pKa = 9.58TALKK89 pKa = 8.76KK90 pKa = 7.35TQKK93 pKa = 9.46TYY95 pKa = 10.21PQNLLNQKK103 pKa = 9.39FNPDD107 pKa = 3.26KK108 pKa = 10.86PNQVWSTDD116 pKa = 3.65FTYY119 pKa = 10.75ISIGYY124 pKa = 8.47KK125 pKa = 9.95KK126 pKa = 10.37YY127 pKa = 11.17VYY129 pKa = 10.64LCTILDD135 pKa = 4.23LYY137 pKa = 10.76SRR139 pKa = 11.84KK140 pKa = 10.07CIAWKK145 pKa = 10.21LSHH148 pKa = 7.08RR149 pKa = 11.84MDD151 pKa = 4.53AKK153 pKa = 10.53LACDD157 pKa = 3.73TLEE160 pKa = 4.07LALNKK165 pKa = 10.27RR166 pKa = 11.84KK167 pKa = 10.31VEE169 pKa = 4.06GTLLFHH175 pKa = 7.31SDD177 pKa = 2.58QGAQFKK183 pKa = 10.65ARR185 pKa = 11.84EE186 pKa = 3.89FRR188 pKa = 11.84KK189 pKa = 10.22IIDD192 pKa = 4.66DD193 pKa = 3.91NNIMHH198 pKa = 6.73SFSKK202 pKa = 10.38PGYY205 pKa = 8.29PYY207 pKa = 11.3DD208 pKa = 3.75NAVTEE213 pKa = 4.71AFFKK217 pKa = 10.46YY218 pKa = 10.01LKK220 pKa = 10.11HH221 pKa = 6.13RR222 pKa = 11.84QINRR226 pKa = 11.84KK227 pKa = 9.05KK228 pKa = 8.6YY229 pKa = 9.5QNIKK233 pKa = 9.86QVQLDD238 pKa = 3.74CFEE241 pKa = 4.87YY242 pKa = 10.61IEE244 pKa = 4.81NFYY247 pKa = 11.54NNYY250 pKa = 9.72NPHH253 pKa = 5.78TANLGLTPNQKK264 pKa = 10.09EE265 pKa = 4.01EE266 pKa = 4.22NYY268 pKa = 10.46FNAIKK273 pKa = 10.62

Molecular weight: 32.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

13720

38

1205

182.9

20.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.713 ± 0.519

0.554 ± 0.088

6.29 ± 0.265

7.427 ± 0.445

3.95 ± 0.163

5.743 ± 0.513

1.487 ± 0.138

6.924 ± 0.264

9.38 ± 0.444

8.717 ± 0.232

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.34 ± 0.149

5.897 ± 0.27

2.631 ± 0.236

4.235 ± 0.231

4.257 ± 0.243

6.327 ± 0.312

6.166 ± 0.331

5.853 ± 0.266

1.064 ± 0.105

4.045 ± 0.348
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski