Gordonia phage SteamedHams

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Emalynvirus; unclassified Emalynvirus

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TRZ9|A0A5J6TRZ9_9CAUD Major tail protein OS=Gordonia phage SteamedHams OX=2599851 GN=15 PE=4 SV=1
MM1 pKa = 7.34TPLEE5 pKa = 4.27KK6 pKa = 10.57LEE8 pKa = 3.76EE9 pKa = 4.81AIRR12 pKa = 11.84EE13 pKa = 4.22FAASQADD20 pKa = 3.76EE21 pKa = 5.31DD22 pKa = 4.88GVTLADD28 pKa = 3.24WFLGYY33 pKa = 9.88SQQQIDD39 pKa = 3.84SDD41 pKa = 4.24GSIQYY46 pKa = 9.43SHH48 pKa = 6.83GYY50 pKa = 8.49AASEE54 pKa = 3.87NVYY57 pKa = 10.76GGVGVAGVALAAARR71 pKa = 11.84GDD73 pKa = 3.67LQGGEE78 pKa = 4.29EE79 pKa = 4.28EE80 pKa = 4.53EE81 pKa = 4.65GLL83 pKa = 4.07

Molecular weight: 8.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TX52|A0A5J6TX52_9CAUD Scaffolding protein OS=Gordonia phage SteamedHams OX=2599851 GN=9 PE=4 SV=1
MM1 pKa = 7.55AKK3 pKa = 9.92KK4 pKa = 10.33KK5 pKa = 10.56RR6 pKa = 11.84GFKK9 pKa = 10.3SKK11 pKa = 10.56KK12 pKa = 5.54QWRR15 pKa = 11.84WAFATKK21 pKa = 10.3QPWARR26 pKa = 11.84RR27 pKa = 11.84WAHH30 pKa = 4.9ATKK33 pKa = 10.34GGKK36 pKa = 6.74KK37 pKa = 7.33TRR39 pKa = 11.84YY40 pKa = 8.74RR41 pKa = 11.84RR42 pKa = 11.84LPRR45 pKa = 11.84KK46 pKa = 8.44VRR48 pKa = 11.84KK49 pKa = 9.34RR50 pKa = 3.37

Molecular weight: 6.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

14117

25

1595

213.9

23.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.634 ± 0.553

0.808 ± 0.142

6.255 ± 0.372

6.219 ± 0.441

3.06 ± 0.195

8.564 ± 0.556

1.948 ± 0.261

4.824 ± 0.245

5.277 ± 0.304

8.075 ± 0.302

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.713 ± 0.157

3.096 ± 0.173

5.376 ± 0.253

3.641 ± 0.201

6.503 ± 0.349

6.014 ± 0.319

5.936 ± 0.307

6.935 ± 0.288

2.224 ± 0.162

2.897 ± 0.255
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski