Amino acid dipepetide frequency for Hyphomicrobium nitrativorans NL23

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.161AlaAla: 18.161 ± 0.217	1.035AlaCys: 1.035 ± 0.036	7.126AlaAsp: 7.126 ± 0.096	8.486AlaGlu: 8.486 ± 0.122	4.671AlaPhe: 4.671 ± 0.07	10.583AlaGly: 10.583 ± 0.105	2.612AlaHis: 2.612 ± 0.053	6.121AlaIle: 6.121 ± 0.089	4.316AlaLys: 4.316 ± 0.082	13.69AlaLeu: 13.69 ± 0.16	3.05AlaMet: 3.05 ± 0.057	2.998AlaAsn: 2.998 ± 0.055	6.364AlaPro: 6.364 ± 0.096	3.732AlaGln: 3.732 ± 0.065	9.593AlaArg: 9.593 ± 0.102	6.99AlaSer: 6.99 ± 0.095	6.285AlaThr: 6.285 ± 0.086	9.255AlaVal: 9.255 ± 0.123	1.559AlaTrp: 1.559 ± 0.041	2.594AlaTyr: 2.594 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	0.931CysAla: 0.931 ± 0.033	0.114CysCys: 0.114 ± 0.013	0.453CysAsp: 0.453 ± 0.022	0.441CysGlu: 0.441 ± 0.019	0.308CysPhe: 0.308 ± 0.015	0.823CysGly: 0.823 ± 0.032	0.226CysHis: 0.226 ± 0.017	0.323CysIle: 0.323 ± 0.019	0.216CysLys: 0.216 ± 0.014	0.695CysLeu: 0.695 ± 0.025	0.142CysMet: 0.142 ± 0.012	0.205CysAsn: 0.205 ± 0.015	0.447CysPro: 0.447 ± 0.026	0.205CysGln: 0.205 ± 0.015	0.643CysArg: 0.643 ± 0.029	0.413CysSer: 0.413 ± 0.021	0.388CysThr: 0.388 ± 0.017	0.593CysVal: 0.593 ± 0.023	0.099CysTrp: 0.099 ± 0.01	0.187CysTyr: 0.187 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	7.572AspAla: 7.572 ± 0.095	0.517AspCys: 0.517 ± 0.024	3.35AspAsp: 3.35 ± 0.073	3.701AspGlu: 3.701 ± 0.066	1.958AspPhe: 1.958 ± 0.054	5.295AspGly: 5.295 ± 0.071	1.127AspHis: 1.127 ± 0.039	2.96AspIle: 2.96 ± 0.053	1.696AspLys: 1.696 ± 0.041	5.947AspLeu: 5.947 ± 0.089	1.179AspMet: 1.179 ± 0.036	1.179AspAsn: 1.179 ± 0.036	3.221AspPro: 3.221 ± 0.059	1.517AspGln: 1.517 ± 0.043	4.258AspArg: 4.258 ± 0.068	1.903AspSer: 1.903 ± 0.041	2.927AspThr: 2.927 ± 0.054	4.679AspVal: 4.679 ± 0.07	0.853AspTrp: 0.853 ± 0.03	1.411AspTyr: 1.411 ± 0.033	0.0AspXaa: 0.0 ± 0.0
Glu	8.345GluAla: 8.345 ± 0.114	0.368GluCys: 0.368 ± 0.022	2.962GluAsp: 2.962 ± 0.064	3.23GluGlu: 3.23 ± 0.065	1.702GluPhe: 1.702 ± 0.039	4.484GluGly: 4.484 ± 0.068	1.222GluHis: 1.222 ± 0.04	3.886GluIle: 3.886 ± 0.069	2.419GluLys: 2.419 ± 0.061	5.379GluLeu: 5.379 ± 0.08	1.436GluMet: 1.436 ± 0.043	1.557GluAsn: 1.557 ± 0.039	2.98GluPro: 2.98 ± 0.058	2.103GluGln: 2.103 ± 0.051	5.805GluArg: 5.805 ± 0.085	2.484GluSer: 2.484 ± 0.051	4.029GluThr: 4.029 ± 0.069	3.985GluVal: 3.985 ± 0.066	0.693GluTrp: 0.693 ± 0.021	0.946GluTyr: 0.946 ± 0.031	0.0GluXaa: 0.0 ± 0.0
Phe	4.548PheAla: 4.548 ± 0.071	0.351PheCys: 0.351 ± 0.019	2.482PheAsp: 2.482 ± 0.049	2.292PheGlu: 2.292 ± 0.05	1.37PhePhe: 1.37 ± 0.042	3.546PheGly: 3.546 ± 0.069	0.744PheHis: 0.744 ± 0.028	1.438PheIle: 1.438 ± 0.037	1.099PheLys: 1.099 ± 0.036	3.221PheLeu: 3.221 ± 0.064	0.738PheMet: 0.738 ± 0.027	0.972PheAsn: 0.972 ± 0.032	1.5PhePro: 1.5 ± 0.036	0.942PheGln: 0.942 ± 0.036	2.327PheArg: 2.327 ± 0.046	2.263PheSer: 2.263 ± 0.046	1.887PheThr: 1.887 ± 0.047	2.848PheVal: 2.848 ± 0.061	0.551PheTrp: 0.551 ± 0.023	0.873PheTyr: 0.873 ± 0.032	0.0PheXaa: 0.0 ± 0.0
Gly	9.897GlyAla: 9.897 ± 0.112	0.794GlyCys: 0.794 ± 0.032	4.457GlyAsp: 4.457 ± 0.069	4.934GlyGlu: 4.934 ± 0.074	3.437GlyPhe: 3.437 ± 0.062	7.486GlyGly: 7.486 ± 0.116	2.011GlyHis: 2.011 ± 0.05	4.251GlyIle: 4.251 ± 0.075	3.111GlyLys: 3.111 ± 0.063	8.671GlyLeu: 8.671 ± 0.094	1.922GlyMet: 1.922 ± 0.045	2.074GlyAsn: 2.074 ± 0.055	3.692GlyPro: 3.692 ± 0.058	2.6GlyGln: 2.6 ± 0.054	6.477GlyArg: 6.477 ± 0.089	4.562GlySer: 4.562 ± 0.076	4.837GlyThr: 4.837 ± 0.075	6.211GlyVal: 6.211 ± 0.096	1.226GlyTrp: 1.226 ± 0.037	2.211GlyTyr: 2.211 ± 0.056	0.001GlyXaa: 0.001 ± 0.001
His	2.467HisAla: 2.467 ± 0.054	0.209HisCys: 0.209 ± 0.015	1.242HisAsp: 1.242 ± 0.037	1.14HisGlu: 1.14 ± 0.034	0.819HisPhe: 0.819 ± 0.031	1.981HisGly: 1.981 ± 0.049	0.539HisHis: 0.539 ± 0.024	1.001HisIle: 1.001 ± 0.033	0.587HisLys: 0.587 ± 0.024	1.987HisLeu: 1.987 ± 0.042	0.487HisMet: 0.487 ± 0.022	0.51HisAsn: 0.51 ± 0.023	1.275HisPro: 1.275 ± 0.042	0.563HisGln: 0.563 ± 0.022	1.532HisArg: 1.532 ± 0.042	0.915HisSer: 0.915 ± 0.034	0.976HisThr: 0.976 ± 0.029	1.646HisVal: 1.646 ± 0.041	0.32HisTrp: 0.32 ± 0.016	0.531HisTyr: 0.531 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	7.285IleAla: 7.285 ± 0.094	0.423IleCys: 0.423 ± 0.021	3.416IleAsp: 3.416 ± 0.064	3.819IleGlu: 3.819 ± 0.069	1.498IlePhe: 1.498 ± 0.044	4.764IleGly: 4.764 ± 0.077	0.852IleHis: 0.852 ± 0.033	1.796IleIle: 1.796 ± 0.047	1.55IleLys: 1.55 ± 0.039	4.035IleLeu: 4.035 ± 0.078	0.911IleMet: 0.911 ± 0.031	1.253IleAsn: 1.253 ± 0.037	2.198IlePro: 2.198 ± 0.048	1.182IleGln: 1.182 ± 0.033	3.266IleArg: 3.266 ± 0.058	2.808IleSer: 2.808 ± 0.054	2.423IleThr: 2.423 ± 0.05	4.517IleVal: 4.517 ± 0.074	0.538IleTrp: 0.538 ± 0.022	1.078IleTyr: 1.078 ± 0.031	0.0IleXaa: 0.0 ± 0.0
Lys	4.59LysAla: 4.59 ± 0.087	0.193LysCys: 0.193 ± 0.015	1.884LysAsp: 1.884 ± 0.044	1.753LysGlu: 1.753 ± 0.053	0.869LysPhe: 0.869 ± 0.03	2.711LysGly: 2.711 ± 0.059	0.636LysHis: 0.636 ± 0.023	1.723LysIle: 1.723 ± 0.045	1.502LysLys: 1.502 ± 0.055	3.356LysLeu: 3.356 ± 0.073	0.701LysMet: 0.701 ± 0.029	0.842LysAsn: 0.842 ± 0.027	2.176LysPro: 2.176 ± 0.048	0.956LysGln: 0.956 ± 0.031	2.561LysArg: 2.561 ± 0.056	2.096LysSer: 2.096 ± 0.046	2.182LysThr: 2.182 ± 0.05	2.339LysVal: 2.339 ± 0.05	0.351LysTrp: 0.351 ± 0.017	0.656LysTyr: 0.656 ± 0.026	0.0LysXaa: 0.0 ± 0.0
Leu	14.053LeuAla: 14.053 ± 0.145	0.674LeuCys: 0.674 ± 0.028	6.152LeuAsp: 6.152 ± 0.087	5.384LeuGlu: 5.384 ± 0.071	3.46LeuPhe: 3.46 ± 0.077	8.041LeuGly: 8.041 ± 0.099	1.785LeuHis: 1.785 ± 0.048	5.003LeuIle: 5.003 ± 0.081	3.937LeuLys: 3.937 ± 0.07	8.846LeuLeu: 8.846 ± 0.126	2.172LeuMet: 2.172 ± 0.05	2.389LeuAsn: 2.389 ± 0.052	5.199LeuPro: 5.199 ± 0.067	2.323LeuGln: 2.323 ± 0.045	6.379LeuArg: 6.379 ± 0.085	6.186LeuSer: 6.186 ± 0.079	5.581LeuThr: 5.581 ± 0.073	7.638LeuVal: 7.638 ± 0.102	1.188LeuTrp: 1.188 ± 0.039	1.992LeuTyr: 1.992 ± 0.041	0.0LeuXaa: 0.0 ± 0.0
Met	2.753MetAla: 2.753 ± 0.056	0.153MetCys: 0.153 ± 0.013	1.007MetAsp: 1.007 ± 0.033	0.997MetGlu: 0.997 ± 0.03	0.65MetPhe: 0.65 ± 0.026	1.658MetGly: 1.658 ± 0.045	0.432MetHis: 0.432 ± 0.021	1.198MetIle: 1.198 ± 0.039	0.929MetLys: 0.929 ± 0.034	2.202MetLeu: 2.202 ± 0.053	0.562MetMet: 0.562 ± 0.027	0.599MetAsn: 0.599 ± 0.026	1.321MetPro: 1.321 ± 0.033	0.763MetGln: 0.763 ± 0.025	1.781MetArg: 1.781 ± 0.046	1.581MetSer: 1.581 ± 0.04	1.85MetThr: 1.85 ± 0.041	1.388MetVal: 1.388 ± 0.041	0.22MetTrp: 0.22 ± 0.017	0.257MetTyr: 0.257 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	3.302AsnAla: 3.302 ± 0.076	0.222AsnCys: 0.222 ± 0.015	1.31AsnAsp: 1.31 ± 0.04	1.299AsnGlu: 1.299 ± 0.039	0.819AsnPhe: 0.819 ± 0.031	2.299AsnGly: 2.299 ± 0.055	0.471AsnHis: 0.471 ± 0.022	1.204AsnIle: 1.204 ± 0.034	0.724AsnLys: 0.724 ± 0.026	2.376AsnLeu: 2.376 ± 0.061	0.552AsnMet: 0.552 ± 0.022	0.635AsnAsn: 0.635 ± 0.033	1.708AsnPro: 1.708 ± 0.049	0.661AsnGln: 0.661 ± 0.031	1.734AsnArg: 1.734 ± 0.036	1.186AsnSer: 1.186 ± 0.046	1.301AsnThr: 1.301 ± 0.038	2.07AsnVal: 2.07 ± 0.047	0.382AsnTrp: 0.382 ± 0.022	0.535AsnTyr: 0.535 ± 0.024	0.0AsnXaa: 0.0 ± 0.0
Pro	6.372ProAla: 6.372 ± 0.099	0.31ProCys: 0.31 ± 0.018	3.533ProAsp: 3.533 ± 0.064	3.86ProGlu: 3.86 ± 0.061	2.033ProPhe: 2.033 ± 0.044	4.58ProGly: 4.58 ± 0.075	1.127ProHis: 1.127 ± 0.032	2.336ProIle: 2.336 ± 0.053	1.801ProLys: 1.801 ± 0.046	4.724ProLeu: 4.724 ± 0.07	1.151ProMet: 1.151 ± 0.033	1.323ProAsn: 1.323 ± 0.037	3.064ProPro: 3.064 ± 0.089	1.651ProGln: 1.651 ± 0.045	3.257ProArg: 3.257 ± 0.065	3.173ProSer: 3.173 ± 0.058	2.452ProThr: 2.452 ± 0.053	4.032ProVal: 4.032 ± 0.071	0.65ProTrp: 0.65 ± 0.03	1.107ProTyr: 1.107 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	3.738GlnAla: 3.738 ± 0.065	0.192GlnCys: 0.192 ± 0.014	1.46GlnAsp: 1.46 ± 0.036	1.437GlnGlu: 1.437 ± 0.038	0.91GlnPhe: 0.91 ± 0.035	2.285GlnGly: 2.285 ± 0.054	0.67GlnHis: 0.67 ± 0.027	1.584GlnIle: 1.584 ± 0.038	1.06GlnLys: 1.06 ± 0.034	2.59GlnLeu: 2.59 ± 0.057	0.72GlnMet: 0.72 ± 0.026	0.799GlnAsn: 0.799 ± 0.032	1.695GlnPro: 1.695 ± 0.051	1.117GlnGln: 1.117 ± 0.051	2.51GlnArg: 2.51 ± 0.055	1.815GlnSer: 1.815 ± 0.045	1.698GlnThr: 1.698 ± 0.04	1.864GlnVal: 1.864 ± 0.049	0.343GlnTrp: 0.343 ± 0.019	0.524GlnTyr: 0.524 ± 0.022	0.0GlnXaa: 0.0 ± 0.0
Arg	8.745ArgAla: 8.745 ± 0.108	0.497ArgCys: 0.497 ± 0.023	4.345ArgAsp: 4.345 ± 0.067	4.574ArgGlu: 4.574 ± 0.071	3.056ArgPhe: 3.056 ± 0.057	5.14ArgGly: 5.14 ± 0.071	1.812ArgHis: 1.812 ± 0.047	4.241ArgIle: 4.241 ± 0.064	2.448ArgLys: 2.448 ± 0.06	8.008ArgLeu: 8.008 ± 0.105	1.825ArgMet: 1.825 ± 0.04	1.975ArgAsn: 1.975 ± 0.05	3.649ArgPro: 3.649 ± 0.063	2.391ArgGln: 2.391 ± 0.048	6.223ArgArg: 6.223 ± 0.1	3.763ArgSer: 3.763 ± 0.07	3.743ArgThr: 3.743 ± 0.067	5.186ArgVal: 5.186 ± 0.081	0.974ArgTrp: 0.974 ± 0.029	1.745ArgTyr: 1.745 ± 0.045	0.0ArgXaa: 0.0 ± 0.0
Ser	6.724SerAla: 6.724 ± 0.084	0.446SerCys: 0.446 ± 0.024	3.113SerAsp: 3.113 ± 0.063	3.306SerGlu: 3.306 ± 0.067	2.125SerPhe: 2.125 ± 0.049	5.631SerGly: 5.631 ± 0.089	1.111SerHis: 1.111 ± 0.033	2.596SerIle: 2.596 ± 0.053	1.722SerLys: 1.722 ± 0.046	5.268SerLeu: 5.268 ± 0.081	1.236SerMet: 1.236 ± 0.037	1.44SerAsn: 1.44 ± 0.042	2.898SerPro: 2.898 ± 0.065	1.604SerGln: 1.604 ± 0.038	3.811SerArg: 3.811 ± 0.065	3.039SerSer: 3.039 ± 0.069	2.762SerThr: 2.762 ± 0.055	3.968SerVal: 3.968 ± 0.063	0.759SerTrp: 0.759 ± 0.028	1.304SerTyr: 1.304 ± 0.043	0.0SerXaa: 0.0 ± 0.0
Thr	6.415ThrAla: 6.415 ± 0.085	0.4ThrCys: 0.4 ± 0.02	2.936ThrAsp: 2.936 ± 0.048	2.919ThrGlu: 2.919 ± 0.057	2.139ThrPhe: 2.139 ± 0.046	5.179ThrGly: 5.179 ± 0.086	1.135ThrHis: 1.135 ± 0.033	2.616ThrIle: 2.616 ± 0.051	1.543ThrLys: 1.543 ± 0.037	6.015ThrLeu: 6.015 ± 0.081	1.096ThrMet: 1.096 ± 0.037	1.31ThrAsn: 1.31 ± 0.041	3.419ThrPro: 3.419 ± 0.055	1.499ThrGln: 1.499 ± 0.042	3.709ThrArg: 3.709 ± 0.067	3.063ThrSer: 3.063 ± 0.057	2.782ThrThr: 2.782 ± 0.065	4.372ThrVal: 4.372 ± 0.071	0.682ThrTrp: 0.682 ± 0.026	1.225ThrTyr: 1.225 ± 0.037	0.0ThrXaa: 0.0 ± 0.0
Val	9.601ValAla: 9.601 ± 0.11	0.592ValCys: 0.592 ± 0.026	4.013ValAsp: 4.013 ± 0.074	4.578ValGlu: 4.578 ± 0.073	2.815ValPhe: 2.815 ± 0.057	5.47ValGly: 5.47 ± 0.092	1.455ValHis: 1.455 ± 0.037	3.737ValIle: 3.737 ± 0.066	2.343ValLys: 2.343 ± 0.052	7.688ValLeu: 7.688 ± 0.099	1.682ValMet: 1.682 ± 0.042	1.807ValAsn: 1.807 ± 0.041	4.066ValPro: 4.066 ± 0.072	1.975ValGln: 1.975 ± 0.041	5.509ValArg: 5.509 ± 0.078	4.726ValSer: 4.726 ± 0.075	4.564ValThr: 4.564 ± 0.073	6.169ValVal: 6.169 ± 0.094	0.974ValTrp: 0.974 ± 0.032	1.513ValTyr: 1.513 ± 0.046	0.0ValXaa: 0.0 ± 0.0
Trp	1.195TrpAla: 1.195 ± 0.034	0.145TrpCys: 0.145 ± 0.013	0.609TrpAsp: 0.609 ± 0.027	0.58TrpGlu: 0.58 ± 0.023	0.488TrpPhe: 0.488 ± 0.024	0.938TrpGly: 0.938 ± 0.028	0.303TrpHis: 0.303 ± 0.018	0.698TrpIle: 0.698 ± 0.03	0.474TrpLys: 0.474 ± 0.023	1.526TrpLeu: 1.526 ± 0.041	0.327TrpMet: 0.327 ± 0.018	0.394TrpAsn: 0.394 ± 0.019	0.69TrpPro: 0.69 ± 0.029	0.527TrpGln: 0.527 ± 0.023	1.188TrpArg: 1.188 ± 0.039	0.862TrpSer: 0.862 ± 0.028	0.738TrpThr: 0.738 ± 0.028	0.804TrpVal: 0.804 ± 0.031	0.208TrpTrp: 0.208 ± 0.016	0.251TrpTyr: 0.251 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.528TyrAla: 2.528 ± 0.05	0.216TyrCys: 0.216 ± 0.014	1.399TyrAsp: 1.399 ± 0.037	1.283TyrGlu: 1.283 ± 0.041	0.865TyrPhe: 0.865 ± 0.03	1.964TyrGly: 1.964 ± 0.047	0.457TyrHis: 0.457 ± 0.022	0.903TyrIle: 0.903 ± 0.029	0.6TyrLys: 0.6 ± 0.024	2.159TyrLeu: 2.159 ± 0.051	0.413TyrMet: 0.413 ± 0.021	0.528TyrAsn: 0.528 ± 0.024	0.974TyrPro: 0.974 ± 0.031	0.701TyrGln: 0.701 ± 0.03	1.778TyrArg: 1.778 ± 0.051	1.036TyrSer: 1.036 ± 0.032	1.047TyrThr: 1.047 ± 0.033	1.711TyrVal: 1.711 ± 0.043	0.376TyrTrp: 0.376 ± 0.019	0.556TyrTyr: 0.556 ± 0.026	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3330 proteins (1004392 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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