Amycolatopsis sulphurea

Taxonomy:

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5941 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A9FAH0|A0A2A9FAH0_9PSEU Porphobilinogen deaminase OS=Amycolatopsis sulphurea OX=76022 GN=hemC PE=3 SV=1
MM1 pKa = 7.38WVDD4 pKa = 3.3EE5 pKa = 4.78SGGSDD10 pKa = 3.28ANGPEE15 pKa = 3.8AMTVHH20 pKa = 6.61VEE22 pKa = 4.03GQEE25 pKa = 3.96YY26 pKa = 8.97QAEE29 pKa = 4.32VNYY32 pKa = 10.8DD33 pKa = 3.47ADD35 pKa = 3.96HH36 pKa = 7.49DD37 pKa = 4.58GVADD41 pKa = 3.78TAVVEE46 pKa = 4.76HH47 pKa = 7.24GDD49 pKa = 3.32GTAQAFLDD57 pKa = 3.89TDD59 pKa = 3.62HH60 pKa = 7.47DD61 pKa = 4.63GVADD65 pKa = 4.51RR66 pKa = 11.84YY67 pKa = 9.85EE68 pKa = 3.86VLGASGQVQDD78 pKa = 3.53QAVYY82 pKa = 10.99DD83 pKa = 4.09EE84 pKa = 5.75ASGSWVEE91 pKa = 4.32SGGHH95 pKa = 5.75SGGTDD100 pKa = 2.92APAQNTSAGGHH111 pKa = 5.21IHH113 pKa = 7.4ADD115 pKa = 3.56LPGGGVDD122 pKa = 4.94AGAASIDD129 pKa = 3.84TDD131 pKa = 3.71HH132 pKa = 7.62DD133 pKa = 3.73GHH135 pKa = 6.79NDD137 pKa = 3.38TVVVEE142 pKa = 4.58TKK144 pKa = 10.63SGGTIAFTDD153 pKa = 3.55TDD155 pKa = 3.83GDD157 pKa = 3.94GAADD161 pKa = 3.5VAVEE165 pKa = 4.11IGSDD169 pKa = 3.6GSTSTYY175 pKa = 9.67EE176 pKa = 3.9HH177 pKa = 7.0TGPGQWSEE185 pKa = 4.05TSGPPDD191 pKa = 3.47RR192 pKa = 11.84AFDD195 pKa = 4.05PAADD199 pKa = 3.94AAWGGEE205 pKa = 4.04GTQLLNGVARR215 pKa = 11.84IDD217 pKa = 3.66SGTGQWISPNN227 pKa = 3.39

Molecular weight: 22.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A9FCR8|A0A2A9FCR8_9PSEU Crotonobetaine/carnitine-CoA ligase OS=Amycolatopsis sulphurea OX=76022 GN=ATK36_4367 PE=4 SV=1
MM1 pKa = 7.67PVPRR5 pKa = 11.84PWRR8 pKa = 11.84LHH10 pKa = 4.53SARR13 pKa = 11.84PGDD16 pKa = 3.56LHH18 pKa = 7.09RR19 pKa = 11.84WRR21 pKa = 11.84ARR23 pKa = 11.84PGPPAPAPPHH33 pKa = 5.85LVRR36 pKa = 11.84HH37 pKa = 5.78GRR39 pKa = 11.84RR40 pKa = 11.84TLRR43 pKa = 11.84PRR45 pKa = 11.84PVPRR49 pKa = 11.84GRR51 pKa = 11.84RR52 pKa = 11.84APAPCRR58 pKa = 11.84ARR60 pKa = 11.84RR61 pKa = 11.84GAAARR66 pKa = 11.84LHH68 pKa = 6.03SRR70 pKa = 11.84ARR72 pKa = 11.84PGTRR76 pKa = 11.84TLPVLRR82 pKa = 11.84SRR84 pKa = 11.84RR85 pKa = 11.84RR86 pKa = 11.84ASRR89 pKa = 11.84GGPQRR94 pKa = 11.84RR95 pKa = 11.84PVRR98 pKa = 11.84TGAEE102 pKa = 4.02LPRR105 pKa = 11.84RR106 pKa = 11.84RR107 pKa = 11.84QVVTRR112 pKa = 11.84RR113 pKa = 11.84ARR115 pKa = 11.84WAVILQAAARR125 pKa = 11.84GTRR128 pKa = 11.84LLMNPRR134 pKa = 11.84VAQLVALRR142 pKa = 11.84AWPVRR147 pKa = 11.84PLVRR151 pKa = 11.84WAPRR155 pKa = 11.84PAPPGRR161 pKa = 11.84GVVARR166 pKa = 11.84APPRR170 pKa = 11.84AGRR173 pKa = 11.84SRR175 pKa = 11.84IPATPRR181 pKa = 11.84VVPARR186 pKa = 11.84PRR188 pKa = 11.84LVIRR192 pKa = 11.84RR193 pKa = 11.84SRR195 pKa = 11.84RR196 pKa = 11.84PSIPQVFRR204 pKa = 11.84PLVRR208 pKa = 11.84AARR211 pKa = 11.84RR212 pKa = 11.84APTPSIRR219 pKa = 11.84QAVQLRR225 pKa = 11.84RR226 pKa = 11.84QVARR230 pKa = 11.84RR231 pKa = 11.84ARR233 pKa = 11.84TPPVPQAAQPPARR246 pKa = 11.84RR247 pKa = 11.84ATRR250 pKa = 11.84LQAGQRR256 pKa = 11.84PAVRR260 pKa = 11.84RR261 pKa = 11.84APRR264 pKa = 11.84SVQRR268 pKa = 11.84ARR270 pKa = 11.84LQVPRR275 pKa = 11.84PAVWVAPRR283 pKa = 11.84TAASPSRR290 pKa = 11.84PSPNPSAIRR299 pKa = 11.84ATSRR303 pKa = 11.84ATVRR307 pKa = 11.84SPVARR312 pKa = 11.84RR313 pKa = 11.84ARR315 pKa = 11.84SRR317 pKa = 11.84GPAAEE322 pKa = 4.04VRR324 pKa = 11.84RR325 pKa = 11.84SPRR328 pKa = 11.84RR329 pKa = 11.84VVSVGRR335 pKa = 11.84RR336 pKa = 11.84AGRR339 pKa = 11.84WRR341 pKa = 11.84PVVRR345 pKa = 11.84AGIRR349 pKa = 11.84AGGPGRR355 pKa = 11.84ARR357 pKa = 11.84RR358 pKa = 11.84RR359 pKa = 11.84CSGMRR364 pKa = 11.84RR365 pKa = 11.84GRR367 pKa = 11.84PAGPVPIPRR376 pKa = 11.84CRR378 pKa = 11.84SRR380 pKa = 11.84VSRR383 pKa = 11.84GRR385 pKa = 11.84PRR387 pKa = 11.84RR388 pKa = 11.84TSSVSTDD395 pKa = 2.9SQARR399 pKa = 11.84KK400 pKa = 8.1VWPVRR405 pKa = 11.84KK406 pKa = 9.7VRR408 pKa = 11.84AFRR411 pKa = 11.84QACQARR417 pKa = 11.84AAA419 pKa = 4.03

Molecular weight: 46.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5941

0

5941

1913325

29

28289

322.1

34.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.273 ± 0.047

0.777 ± 0.014

5.795 ± 0.027

5.672 ± 0.037

2.866 ± 0.018

9.434 ± 0.033

2.262 ± 0.015

3.394 ± 0.026

2.211 ± 0.023

10.372 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.64 ± 0.014

1.872 ± 0.018

6.157 ± 0.035

2.959 ± 0.017

7.934 ± 0.036

5.254 ± 0.038

6.024 ± 0.027

8.646 ± 0.032

1.465 ± 0.012

1.993 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski