Amino acid dipepetide frequency for Orbus hercynius

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.462AlaAla: 6.462 ± 0.122
0.95AlaCys: 0.95 ± 0.037
4.277AlaAsp: 4.277 ± 0.087
4.338AlaGlu: 4.338 ± 0.088
3.122AlaPhe: 3.122 ± 0.079
5.127AlaGly: 5.127 ± 0.107
1.498AlaHis: 1.498 ± 0.052
6.974AlaIle: 6.974 ± 0.094
5.405AlaLys: 5.405 ± 0.103
9.071AlaLeu: 9.071 ± 0.117
2.263AlaMet: 2.263 ± 0.06
4.071AlaAsn: 4.071 ± 0.071
2.401AlaPro: 2.401 ± 0.062
3.945AlaGln: 3.945 ± 0.079
3.283AlaArg: 3.283 ± 0.079
4.64AlaSer: 4.64 ± 0.085
4.251AlaThr: 4.251 ± 0.084
5.232AlaVal: 5.232 ± 0.094
0.871AlaTrp: 0.871 ± 0.039
2.496AlaTyr: 2.496 ± 0.074
0.0AlaXaa: 0.0 ± 0.0
Cys
0.869CysAla: 0.869 ± 0.033
0.217CysCys: 0.217 ± 0.02
0.733CysAsp: 0.733 ± 0.032
0.532CysGlu: 0.532 ± 0.027
0.552CysPhe: 0.552 ± 0.033
0.885CysGly: 0.885 ± 0.038
0.381CysHis: 0.381 ± 0.027
0.727CysIle: 0.727 ± 0.033
0.439CysLys: 0.439 ± 0.024
1.121CysLeu: 1.121 ± 0.045
0.174CysMet: 0.174 ± 0.018
0.396CysAsn: 0.396 ± 0.027
0.453CysPro: 0.453 ± 0.026
0.603CysGln: 0.603 ± 0.027
0.446CysArg: 0.446 ± 0.026
0.727CysSer: 0.727 ± 0.031
0.439CysThr: 0.439 ± 0.027
0.721CysVal: 0.721 ± 0.03
0.136CysTrp: 0.136 ± 0.012
0.484CysTyr: 0.484 ± 0.027
0.0CysXaa: 0.0 ± 0.0
Asp
3.971AspAla: 3.971 ± 0.076
0.562AspCys: 0.562 ± 0.028
3.134AspAsp: 3.134 ± 0.082
3.696AspGlu: 3.696 ± 0.085
2.466AspPhe: 2.466 ± 0.071
3.329AspGly: 3.329 ± 0.084
1.121AspHis: 1.121 ± 0.044
4.74AspIle: 4.74 ± 0.086
3.725AspLys: 3.725 ± 0.086
5.126AspLeu: 5.126 ± 0.082
1.333AspMet: 1.333 ± 0.041
3.121AspAsn: 3.121 ± 0.077
1.923AspPro: 1.923 ± 0.065
1.824AspGln: 1.824 ± 0.051
2.149AspArg: 2.149 ± 0.061
3.196AspSer: 3.196 ± 0.068
2.414AspThr: 2.414 ± 0.057
3.634AspVal: 3.634 ± 0.082
0.729AspTrp: 0.729 ± 0.034
2.267AspTyr: 2.267 ± 0.067
0.0AspXaa: 0.0 ± 0.0
Glu
3.836GluAla: 3.836 ± 0.096
0.433GluCys: 0.433 ± 0.027
2.178GluAsp: 2.178 ± 0.068
2.386GluGlu: 2.386 ± 0.08
1.999GluPhe: 1.999 ± 0.053
2.676GluGly: 2.676 ± 0.079
1.405GluHis: 1.405 ± 0.045
4.025GluIle: 4.025 ± 0.073
3.583GluLys: 3.583 ± 0.085
6.349GluLeu: 6.349 ± 0.115
1.491GluMet: 1.491 ± 0.05
2.608GluAsn: 2.608 ± 0.054
1.695GluPro: 1.695 ± 0.054
3.829GluGln: 3.829 ± 0.082
2.74GluArg: 2.74 ± 0.064
2.924GluSer: 2.924 ± 0.077
2.463GluThr: 2.463 ± 0.065
3.14GluVal: 3.14 ± 0.073
0.553GluTrp: 0.553 ± 0.028
1.701GluTyr: 1.701 ± 0.054
0.0GluXaa: 0.0 ± 0.0
Phe
3.448PheAla: 3.448 ± 0.082
0.572PheCys: 0.572 ± 0.032
2.843PheAsp: 2.843 ± 0.068
1.982PheGlu: 1.982 ± 0.057
1.927PhePhe: 1.927 ± 0.068
2.97PheGly: 2.97 ± 0.07
0.758PheHis: 0.758 ± 0.034
3.732PheIle: 3.732 ± 0.094
2.118PheLys: 2.118 ± 0.057
3.606PheLeu: 3.606 ± 0.08
1.068PheMet: 1.068 ± 0.038
2.524PheAsn: 2.524 ± 0.065
1.33PhePro: 1.33 ± 0.046
1.063PheGln: 1.063 ± 0.038
1.353PheArg: 1.353 ± 0.042
3.145PheSer: 3.145 ± 0.07
2.425PheThr: 2.425 ± 0.057
2.674PheVal: 2.674 ± 0.062
0.551PheTrp: 0.551 ± 0.031
1.66PheTyr: 1.66 ± 0.058
0.0PheXaa: 0.0 ± 0.0
Gly
4.575GlyAla: 4.575 ± 0.093
0.945GlyCys: 0.945 ± 0.041
3.326GlyAsp: 3.326 ± 0.077
3.542GlyGlu: 3.542 ± 0.085
3.06GlyPhe: 3.06 ± 0.08
4.384GlyGly: 4.384 ± 0.1
1.447GlyHis: 1.447 ± 0.048
5.595GlyIle: 5.595 ± 0.107
4.009GlyLys: 4.009 ± 0.082
6.511GlyLeu: 6.511 ± 0.117
1.796GlyMet: 1.796 ± 0.062
2.753GlyAsn: 2.753 ± 0.083
1.511GlyPro: 1.511 ± 0.051
2.715GlyGln: 2.715 ± 0.065
2.677GlyArg: 2.677 ± 0.065
3.755GlySer: 3.755 ± 0.084
3.192GlyThr: 3.192 ± 0.077
4.578GlyVal: 4.578 ± 0.093
0.826GlyTrp: 0.826 ± 0.036
2.657GlyTyr: 2.657 ± 0.066
0.0GlyXaa: 0.0 ± 0.0
His
1.482HisAla: 1.482 ± 0.046
0.356HisCys: 0.356 ± 0.023
1.205HisAsp: 1.205 ± 0.039
0.956HisGlu: 0.956 ± 0.039
1.097HisPhe: 1.097 ± 0.044
1.398HisGly: 1.398 ± 0.045
0.784HisHis: 0.784 ± 0.042
1.718HisIle: 1.718 ± 0.053
1.098HisLys: 1.098 ± 0.039
2.322HisLeu: 2.322 ± 0.062
0.409HisMet: 0.409 ± 0.026
1.169HisAsn: 1.169 ± 0.047
1.008HisPro: 1.008 ± 0.037
1.281HisGln: 1.281 ± 0.048
0.981HisArg: 0.981 ± 0.037
1.292HisSer: 1.292 ± 0.043
1.0HisThr: 1.0 ± 0.037
1.326HisVal: 1.326 ± 0.048
0.309HisTrp: 0.309 ± 0.022
1.107HisTyr: 1.107 ± 0.05
0.0HisXaa: 0.0 ± 0.0
Ile
7.745IleAla: 7.745 ± 0.121
0.923IleCys: 0.923 ± 0.042
5.288IleAsp: 5.288 ± 0.1
5.072IleGlu: 5.072 ± 0.086
3.132IlePhe: 3.132 ± 0.079
5.52IleGly: 5.52 ± 0.095
1.426IleHis: 1.426 ± 0.05
6.428IleIle: 6.428 ± 0.131
4.748IleLys: 4.748 ± 0.093
7.174IleLeu: 7.174 ± 0.111
1.873IleMet: 1.873 ± 0.055
4.335IleAsn: 4.335 ± 0.085
3.083IlePro: 3.083 ± 0.068
2.48IleGln: 2.48 ± 0.06
3.013IleArg: 3.013 ± 0.07
5.127IleSer: 5.127 ± 0.101
4.891IleThr: 4.891 ± 0.093
5.095IleVal: 5.095 ± 0.111
0.714IleTrp: 0.714 ± 0.033
2.49IleTyr: 2.49 ± 0.065
0.0IleXaa: 0.0 ± 0.0
Lys
4.7LysAla: 4.7 ± 0.088
0.367LysCys: 0.367 ± 0.023
2.876LysAsp: 2.876 ± 0.073
3.095LysGlu: 3.095 ± 0.081
1.602LysPhe: 1.602 ± 0.051
3.306LysGly: 3.306 ± 0.077
1.392LysHis: 1.392 ± 0.046
4.354LysIle: 4.354 ± 0.082
3.707LysLys: 3.707 ± 0.097
6.05LysLeu: 6.05 ± 0.093
1.626LysMet: 1.626 ± 0.044
3.057LysAsn: 3.057 ± 0.076
2.398LysPro: 2.398 ± 0.058
3.696LysGln: 3.696 ± 0.074
2.735LysArg: 2.735 ± 0.066
3.329LysSer: 3.329 ± 0.077
3.421LysThr: 3.421 ± 0.073
3.538LysVal: 3.538 ± 0.082
0.574LysTrp: 0.574 ± 0.027
1.737LysTyr: 1.737 ± 0.052
0.0LysXaa: 0.0 ± 0.0
Leu
9.536LeuAla: 9.536 ± 0.12
1.195LeuCys: 1.195 ± 0.045
5.618LeuAsp: 5.618 ± 0.098
4.797LeuGlu: 4.797 ± 0.105
4.61LeuPhe: 4.61 ± 0.095
6.533LeuGly: 6.533 ± 0.107
1.93LeuHis: 1.93 ± 0.056
7.994LeuIle: 7.994 ± 0.148
5.629LeuLys: 5.629 ± 0.105
11.076LeuLeu: 11.076 ± 0.202
2.514LeuMet: 2.514 ± 0.07
5.2LeuAsn: 5.2 ± 0.117
4.852LeuPro: 4.852 ± 0.083
4.002LeuGln: 4.002 ± 0.086
3.962LeuArg: 3.962 ± 0.084
8.349LeuSer: 8.349 ± 0.137
6.68LeuThr: 6.68 ± 0.102
6.785LeuVal: 6.785 ± 0.116
1.049LeuTrp: 1.049 ± 0.045
3.129LeuTyr: 3.129 ± 0.068
0.0LeuXaa: 0.0 ± 0.0
Met
2.124MetAla: 2.124 ± 0.054
0.229MetCys: 0.229 ± 0.019
1.079MetAsp: 1.079 ± 0.037
0.926MetGlu: 0.926 ± 0.035
0.885MetPhe: 0.885 ± 0.036
1.534MetGly: 1.534 ± 0.05
0.468MetHis: 0.468 ± 0.026
2.046MetIle: 2.046 ± 0.058
1.517MetLys: 1.517 ± 0.045
2.811MetLeu: 2.811 ± 0.068
0.758MetMet: 0.758 ± 0.042
1.231MetAsn: 1.231 ± 0.042
1.16MetPro: 1.16 ± 0.038
1.256MetGln: 1.256 ± 0.042
1.068MetArg: 1.068 ± 0.038
1.814MetSer: 1.814 ± 0.048
1.668MetThr: 1.668 ± 0.049
1.659MetVal: 1.659 ± 0.051
0.217MetTrp: 0.217 ± 0.019
0.51MetTyr: 0.51 ± 0.035
0.0MetXaa: 0.0 ± 0.0
Asn
3.68AsnAla: 3.68 ± 0.083
0.481AsnCys: 0.481 ± 0.029
2.787AsnAsp: 2.787 ± 0.068
2.718AsnGlu: 2.718 ± 0.068
1.62AsnPhe: 1.62 ± 0.053
3.18AsnGly: 3.18 ± 0.079
1.259AsnHis: 1.259 ± 0.043
4.112AsnIle: 4.112 ± 0.072
3.015AsnLys: 3.015 ± 0.072
4.397AsnLeu: 4.397 ± 0.074
1.174AsnMet: 1.174 ± 0.04
3.006AsnAsn: 3.006 ± 0.083
2.156AsnPro: 2.156 ± 0.059
2.819AsnGln: 2.819 ± 0.076
2.17AsnArg: 2.17 ± 0.048
2.966AsnSer: 2.966 ± 0.071
2.596AsnThr: 2.596 ± 0.071
2.885AsnVal: 2.885 ± 0.068
0.69AsnTrp: 0.69 ± 0.028
1.995AsnTyr: 1.995 ± 0.064
0.0AsnXaa: 0.0 ± 0.0
Pro
2.905ProAla: 2.905 ± 0.072
0.359ProCys: 0.359 ± 0.023
2.172ProAsp: 2.172 ± 0.06
2.337ProGlu: 2.337 ± 0.065
1.753ProPhe: 1.753 ± 0.049
1.799ProGly: 1.799 ± 0.061
0.856ProHis: 0.856 ± 0.033
3.071ProIle: 3.071 ± 0.074
2.088ProLys: 2.088 ± 0.059
4.045ProLeu: 4.045 ± 0.074
0.939ProMet: 0.939 ± 0.037
2.072ProAsn: 2.072 ± 0.065
1.037ProPro: 1.037 ± 0.041
1.534ProGln: 1.534 ± 0.044
1.178ProArg: 1.178 ± 0.04
2.341ProSer: 2.341 ± 0.055
2.306ProThr: 2.306 ± 0.06
2.672ProVal: 2.672 ± 0.069
0.393ProTrp: 0.393 ± 0.026
1.507ProTyr: 1.507 ± 0.047
0.0ProXaa: 0.0 ± 0.0
Gln
4.207GlnAla: 4.207 ± 0.081
0.406GlnCys: 0.406 ± 0.024
2.312GlnAsp: 2.312 ± 0.05
2.179GlnGlu: 2.179 ± 0.058
2.015GlnPhe: 2.015 ± 0.052
2.871GlnGly: 2.871 ± 0.069
1.246GlnHis: 1.246 ± 0.048
3.367GlnIle: 3.367 ± 0.07
2.527GlnLys: 2.527 ± 0.065
5.757GlnLeu: 5.757 ± 0.11
1.114GlnMet: 1.114 ± 0.036
2.078GlnAsn: 2.078 ± 0.054
1.611GlnPro: 1.611 ± 0.054
3.312GlnGln: 3.312 ± 0.106
2.088GlnArg: 2.088 ± 0.059
2.866GlnSer: 2.866 ± 0.068
2.554GlnThr: 2.554 ± 0.077
2.841GlnVal: 2.841 ± 0.067
0.565GlnTrp: 0.565 ± 0.026
2.002GlnTyr: 2.002 ± 0.06
0.0GlnXaa: 0.0 ± 0.0
Arg
2.845ArgAla: 2.845 ± 0.067
0.442ArgCys: 0.442 ± 0.026
2.149ArgAsp: 2.149 ± 0.057
2.259ArgGlu: 2.259 ± 0.071
2.078ArgPhe: 2.078 ± 0.052
2.257ArgGly: 2.257 ± 0.052
1.108ArgHis: 1.108 ± 0.04
3.312ArgIle: 3.312 ± 0.077
2.122ArgLys: 2.122 ± 0.059
4.603ArgLeu: 4.603 ± 0.102
1.062ArgMet: 1.062 ± 0.036
1.82ArgAsn: 1.82 ± 0.053
1.373ArgPro: 1.373 ± 0.044
2.233ArgGln: 2.233 ± 0.067
1.967ArgArg: 1.967 ± 0.059
2.262ArgSer: 2.262 ± 0.056
1.944ArgThr: 1.944 ± 0.061
2.782ArgVal: 2.782 ± 0.08
0.488ArgTrp: 0.488 ± 0.028
1.972ArgTyr: 1.972 ± 0.053
0.0ArgXaa: 0.0 ± 0.0
Ser
5.294SerAla: 5.294 ± 0.091
0.637SerCys: 0.637 ± 0.03
3.409SerAsp: 3.409 ± 0.06
3.037SerGlu: 3.037 ± 0.069
2.708SerPhe: 2.708 ± 0.072
4.714SerGly: 4.714 ± 0.106
1.489SerHis: 1.489 ± 0.044
4.837SerIle: 4.837 ± 0.092
3.128SerLys: 3.128 ± 0.069
7.082SerLeu: 7.082 ± 0.119
1.553SerMet: 1.553 ± 0.056
2.75SerAsn: 2.75 ± 0.071
2.309SerPro: 2.309 ± 0.059
2.966SerGln: 2.966 ± 0.068
2.554SerArg: 2.554 ± 0.062
4.193SerSer: 4.193 ± 0.095
3.423SerThr: 3.423 ± 0.076
4.197SerVal: 4.197 ± 0.078
0.684SerTrp: 0.684 ± 0.033
2.347SerTyr: 2.347 ± 0.064
0.0SerXaa: 0.0 ± 0.0
Thr
4.438ThrAla: 4.438 ± 0.089
0.519ThrCys: 0.519 ± 0.033
2.974ThrAsp: 2.974 ± 0.068
2.758ThrGlu: 2.758 ± 0.062
2.154ThrPhe: 2.154 ± 0.057
3.823ThrGly: 3.823 ± 0.085
1.279ThrHis: 1.279 ± 0.042
4.622ThrIle: 4.622 ± 0.094
2.75ThrLys: 2.75 ± 0.06
6.362ThrLeu: 6.362 ± 0.102
1.169ThrMet: 1.169 ± 0.044
2.324ThrAsn: 2.324 ± 0.063
2.659ThrPro: 2.659 ± 0.06
2.596ThrGln: 2.596 ± 0.071
1.995ThrArg: 1.995 ± 0.052
3.179ThrSer: 3.179 ± 0.079
3.199ThrThr: 3.199 ± 0.081
3.691ThrVal: 3.691 ± 0.071
0.59ThrTrp: 0.59 ± 0.029
1.666ThrTyr: 1.666 ± 0.052
0.0ThrXaa: 0.0 ± 0.0
Val
5.297ValAla: 5.297 ± 0.101
0.752ValCys: 0.752 ± 0.035
3.657ValAsp: 3.657 ± 0.084
3.619ValGlu: 3.619 ± 0.077
2.631ValPhe: 2.631 ± 0.068
4.522ValGly: 4.522 ± 0.094
1.104ValHis: 1.104 ± 0.037
5.704ValIle: 5.704 ± 0.099
4.057ValLys: 4.057 ± 0.073
6.392ValLeu: 6.392 ± 0.098
1.705ValMet: 1.705 ± 0.046
3.17ValAsn: 3.17 ± 0.078
2.434ValPro: 2.434 ± 0.072
2.059ValGln: 2.059 ± 0.053
2.38ValArg: 2.38 ± 0.064
4.348ValSer: 4.348 ± 0.075
3.76ValThr: 3.76 ± 0.084
4.53ValVal: 4.53 ± 0.098
0.635ValTrp: 0.635 ± 0.029
1.915ValTyr: 1.915 ± 0.053
0.0ValXaa: 0.0 ± 0.0
Trp
0.704TrpAla: 0.704 ± 0.03
0.148TrpCys: 0.148 ± 0.017
0.484TrpAsp: 0.484 ± 0.03
0.368TrpGlu: 0.368 ± 0.024
0.604TrpPhe: 0.604 ± 0.027
0.716TrpGly: 0.716 ± 0.031
0.306TrpHis: 0.306 ± 0.022
0.79TrpIle: 0.79 ± 0.036
0.409TrpLys: 0.409 ± 0.029
1.589TrpLeu: 1.589 ± 0.047
0.277TrpMet: 0.277 ± 0.02
0.491TrpAsn: 0.491 ± 0.026
0.401TrpPro: 0.401 ± 0.024
1.063TrpGln: 1.063 ± 0.045
0.568TrpArg: 0.568 ± 0.026
0.648TrpSer: 0.648 ± 0.032
0.432TrpThr: 0.432 ± 0.021
0.656TrpVal: 0.656 ± 0.029
0.114TrpTrp: 0.114 ± 0.014
0.4TrpTyr: 0.4 ± 0.029
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.557TyrAla: 2.557 ± 0.067
0.543TyrCys: 0.543 ± 0.026
1.957TyrAsp: 1.957 ± 0.057
1.417TyrGlu: 1.417 ± 0.049
1.734TyrPhe: 1.734 ± 0.06
2.309TyrGly: 2.309 ± 0.061
1.04TyrHis: 1.04 ± 0.042
2.411TyrIle: 2.411 ± 0.068
1.486TyrLys: 1.486 ± 0.052
3.997TyrLeu: 3.997 ± 0.094
0.664TyrMet: 0.664 ± 0.033
1.531TyrAsn: 1.531 ± 0.051
1.569TyrPro: 1.569 ± 0.051
2.643TyrGln: 2.643 ± 0.076
1.769TyrArg: 1.769 ± 0.059
2.211TyrSer: 2.211 ± 0.056
1.689TyrThr: 1.689 ± 0.047
2.034TyrVal: 2.034 ± 0.063
0.481TyrTrp: 0.481 ± 0.027
1.394TyrTyr: 1.394 ± 0.045
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2085 proteins (690237 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski