Histophilus somni (strain 2336) (Haemophilus somnus)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Histophilus; Histophilus somni

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1971 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|B0UWR3|DNAJ_HISS2 Chaperone protein DnaJ OS=Histophilus somni (strain 2336) OX=228400 GN=dnaJ PE=3 SV=1
MM1 pKa = 7.65AIVGLFYY8 pKa = 11.01GSDD11 pKa = 3.2TGNTEE16 pKa = 4.47NIAKK20 pKa = 9.24MIQKK24 pKa = 9.59QLGSEE29 pKa = 4.49LIDD32 pKa = 3.17IRR34 pKa = 11.84DD35 pKa = 3.35IAKK38 pKa = 10.31SSKK41 pKa = 10.43EE42 pKa = 4.02DD43 pKa = 3.21IEE45 pKa = 5.67SYY47 pKa = 10.87DD48 pKa = 3.5FLMIGIPTWYY58 pKa = 9.99YY59 pKa = 11.6GEE61 pKa = 4.69AQCDD65 pKa = 3.26WDD67 pKa = 5.24DD68 pKa = 4.09FFPTLEE74 pKa = 4.91EE75 pKa = 4.17IDD77 pKa = 4.0FTDD80 pKa = 3.19KK81 pKa = 10.88LVAIFGCGDD90 pKa = 3.25QEE92 pKa = 5.04DD93 pKa = 4.3YY94 pKa = 11.79AEE96 pKa = 4.32YY97 pKa = 10.5FCDD100 pKa = 4.34AMGTVRR106 pKa = 11.84NIIEE110 pKa = 4.26PKK112 pKa = 8.68GAIIVGHH119 pKa = 6.89WPTEE123 pKa = 3.95GYY125 pKa = 8.67TFEE128 pKa = 4.28VSQALVDD135 pKa = 4.36DD136 pKa = 4.01NTFVGLCIDD145 pKa = 4.19EE146 pKa = 4.82DD147 pKa = 3.92RR148 pKa = 11.84QPEE151 pKa = 4.14LTAEE155 pKa = 4.16RR156 pKa = 11.84VEE158 pKa = 4.08KK159 pKa = 9.67WVKK162 pKa = 9.55QVYY165 pKa = 10.7DD166 pKa = 4.08EE167 pKa = 4.4MCLAEE172 pKa = 4.39LAA174 pKa = 4.24

Molecular weight: 19.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B0URV6|B0URV6_HISS2 Competence protein OS=Histophilus somni (strain 2336) OX=228400 GN=HSM_0511 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.68RR12 pKa = 11.84NRR14 pKa = 11.84THH16 pKa = 7.28GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.15GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1971

0

1971

655091

32

4656

332.4

37.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.916 ± 0.064

0.98 ± 0.027

5.024 ± 0.043

6.303 ± 0.053

4.277 ± 0.049

6.822 ± 0.096

2.057 ± 0.032

7.427 ± 0.054

6.765 ± 0.082

10.203 ± 0.092

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.3 ± 0.039

5.146 ± 0.07

3.462 ± 0.045

4.815 ± 0.063

4.216 ± 0.067

6.096 ± 0.064

5.443 ± 0.076

6.466 ± 0.046

1.073 ± 0.025

3.207 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski