Lachnospiraceae bacterium XPB1003

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 5.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2824 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G5AW09|A0A1G5AW09_9FIRM Isoleucine--tRNA ligase OS=Lachnospiraceae bacterium XPB1003 OX=1520825 GN=ileS PE=3 SV=1
MM1 pKa = 7.71KK2 pKa = 10.06KK3 pKa = 9.69RR4 pKa = 11.84NRR6 pKa = 11.84MISAALTMMAALSLTACAGTADD28 pKa = 3.87QGSVQTPDD36 pKa = 2.94TAAQAGDD43 pKa = 3.52VTEE46 pKa = 4.25QTTGATDD53 pKa = 3.43EE54 pKa = 4.42VEE56 pKa = 3.91EE57 pKa = 4.43GTVYY61 pKa = 10.85KK62 pKa = 10.42IGVCNYY68 pKa = 10.03VDD70 pKa = 4.23DD71 pKa = 5.64ASLNQIYY78 pKa = 10.58EE79 pKa = 4.24NIEE82 pKa = 3.79AQLDD86 pKa = 3.91VLGSEE91 pKa = 3.94QGVTYY96 pKa = 10.02EE97 pKa = 4.98IVYY100 pKa = 10.33DD101 pKa = 3.88NCNADD106 pKa = 3.88ANVMNQIIEE115 pKa = 4.13NFISGEE121 pKa = 4.13KK122 pKa = 9.15VDD124 pKa = 4.46LMVGIATPVAMTMQSATEE142 pKa = 3.96DD143 pKa = 3.48TQIPVIFSAVSDD155 pKa = 4.1PLSAGLVDD163 pKa = 5.08SLDD166 pKa = 3.94APGHH170 pKa = 6.27NITGTSDD177 pKa = 3.47YY178 pKa = 10.8LNTDD182 pKa = 3.86AIMDD186 pKa = 4.25LMFEE190 pKa = 4.86ADD192 pKa = 4.0PDD194 pKa = 3.79IQTVGLLYY202 pKa = 11.1DD203 pKa = 3.99MGQDD207 pKa = 3.35ASTSAINDD215 pKa = 3.09AKK217 pKa = 11.01AYY219 pKa = 10.72LDD221 pKa = 3.58AKK223 pKa = 9.14GIKK226 pKa = 9.58YY227 pKa = 9.88IEE229 pKa = 4.18RR230 pKa = 11.84TGTTVDD236 pKa = 3.65EE237 pKa = 4.49VMLAADD243 pKa = 4.55TLAKK247 pKa = 9.83SDD249 pKa = 3.68KK250 pKa = 9.98VDD252 pKa = 3.71AVFTPTDD259 pKa = 3.64NTIMTAEE266 pKa = 4.09LSIYY270 pKa = 7.91EE271 pKa = 4.31TFAEE275 pKa = 4.33AGIPHH280 pKa = 6.55YY281 pKa = 10.91CGADD285 pKa = 3.14SFALNGAFLGFGVDD299 pKa = 3.59YY300 pKa = 11.37ANIGVEE306 pKa = 4.17TANMVSEE313 pKa = 4.08VLYY316 pKa = 10.32EE317 pKa = 4.5GKK319 pKa = 10.27DD320 pKa = 3.39PATLGVKK327 pKa = 9.3TFDD330 pKa = 4.28NGIATINTEE339 pKa = 3.82TCDD342 pKa = 4.18AIGFDD347 pKa = 4.39LEE349 pKa = 4.61TVKK352 pKa = 10.87KK353 pKa = 10.5AFEE356 pKa = 3.99PHH358 pKa = 6.01CTDD361 pKa = 2.53IVEE364 pKa = 4.15ITTAEE369 pKa = 4.09EE370 pKa = 4.01FF371 pKa = 3.66

Molecular weight: 39.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G5AV13|A0A1G5AV13_9FIRM DHH family protein OS=Lachnospiraceae bacterium XPB1003 OX=1520825 GN=SAMN02910370_00310 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.73MTFQPKK8 pKa = 8.63NRR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 8.89VHH16 pKa = 5.88GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.71VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2824

0

2824

956784

39

2420

338.8

37.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.549 ± 0.05

1.438 ± 0.017

6.709 ± 0.045

7.524 ± 0.05

4.314 ± 0.034

7.151 ± 0.039

1.605 ± 0.018

7.67 ± 0.043

6.992 ± 0.042

8.375 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.066 ± 0.023

4.36 ± 0.03

3.263 ± 0.025

2.346 ± 0.024

4.42 ± 0.036

6.242 ± 0.041

5.132 ± 0.031

6.758 ± 0.037

0.853 ± 0.015

4.232 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski