Amino acid dipepetide frequency for Mycobacterium phage Phipps

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.965AlaAla: 17.965 ± 1.36
1.313AlaCys: 1.313 ± 0.272
7.083AlaAsp: 7.083 ± 0.557
7.693AlaGlu: 7.693 ± 0.771
2.345AlaPhe: 2.345 ± 0.355
10.46AlaGly: 10.46 ± 1.24
2.439AlaHis: 2.439 ± 0.415
4.503AlaIle: 4.503 ± 0.605
3.471AlaLys: 3.471 ± 0.455
10.695AlaLeu: 10.695 ± 0.964
3.33AlaMet: 3.33 ± 0.351
3.002AlaAsn: 3.002 ± 0.366
6.801AlaPro: 6.801 ± 0.478
4.081AlaGln: 4.081 ± 0.467
8.959AlaArg: 8.959 ± 0.797
5.676AlaSer: 5.676 ± 0.555
7.88AlaThr: 7.88 ± 0.629
9.147AlaVal: 9.147 ± 0.921
2.017AlaTrp: 2.017 ± 0.323
2.955AlaTyr: 2.955 ± 0.318
0.0AlaXaa: 0.0 ± 0.0
Cys
0.751CysAla: 0.751 ± 0.19
0.047CysCys: 0.047 ± 0.044
0.985CysAsp: 0.985 ± 0.22
0.844CysGlu: 0.844 ± 0.246
0.328CysPhe: 0.328 ± 0.111
1.454CysGly: 1.454 ± 0.301
0.328CysHis: 0.328 ± 0.119
0.328CysIle: 0.328 ± 0.134
0.281CysLys: 0.281 ± 0.104
0.704CysLeu: 0.704 ± 0.198
0.188CysMet: 0.188 ± 0.1
0.281CysAsn: 0.281 ± 0.1
0.891CysPro: 0.891 ± 0.237
0.281CysGln: 0.281 ± 0.111
0.985CysArg: 0.985 ± 0.272
0.563CysSer: 0.563 ± 0.157
0.563CysThr: 0.563 ± 0.172
0.844CysVal: 0.844 ± 0.208
0.235CysTrp: 0.235 ± 0.13
0.328CysTyr: 0.328 ± 0.107
0.0CysXaa: 0.0 ± 0.0
Asp
7.224AspAla: 7.224 ± 0.571
0.985AspCys: 0.985 ± 0.266
4.691AspAsp: 4.691 ± 0.647
5.019AspGlu: 5.019 ± 0.602
1.689AspPhe: 1.689 ± 0.248
6.145AspGly: 6.145 ± 0.695
1.36AspHis: 1.36 ± 0.263
3.143AspIle: 3.143 ± 0.364
1.923AspLys: 1.923 ± 0.365
5.629AspLeu: 5.629 ± 0.468
1.079AspMet: 1.079 ± 0.286
2.111AspAsn: 2.111 ± 0.316
5.207AspPro: 5.207 ± 0.498
1.501AspGln: 1.501 ± 0.276
3.846AspArg: 3.846 ± 0.487
2.674AspSer: 2.674 ± 0.347
3.659AspThr: 3.659 ± 0.423
3.799AspVal: 3.799 ± 0.352
1.173AspTrp: 1.173 ± 0.226
1.736AspTyr: 1.736 ± 0.285
0.0AspXaa: 0.0 ± 0.0
Glu
7.693GluAla: 7.693 ± 0.824
0.751GluCys: 0.751 ± 0.228
3.659GluAsp: 3.659 ± 0.643
2.158GluGlu: 2.158 ± 0.301
2.205GluPhe: 2.205 ± 0.378
4.456GluGly: 4.456 ± 0.524
1.22GluHis: 1.22 ± 0.228
3.846GluIle: 3.846 ± 0.535
1.736GluLys: 1.736 ± 0.308
6.332GluLeu: 6.332 ± 0.584
0.844GluMet: 0.844 ± 0.239
1.032GluAsn: 1.032 ± 0.214
3.471GluPro: 3.471 ± 0.527
2.58GluGln: 2.58 ± 0.327
4.268GluArg: 4.268 ± 0.482
1.642GluSer: 1.642 ± 0.325
3.565GluThr: 3.565 ± 0.437
6.051GluVal: 6.051 ± 0.579
1.032GluTrp: 1.032 ± 0.23
1.22GluTyr: 1.22 ± 0.226
0.0GluXaa: 0.0 ± 0.0
Phe
2.955PheAla: 2.955 ± 0.32
0.281PheCys: 0.281 ± 0.104
1.454PheAsp: 1.454 ± 0.257
1.36PheGlu: 1.36 ± 0.224
0.422PhePhe: 0.422 ± 0.13
3.002PheGly: 3.002 ± 0.445
0.516PheHis: 0.516 ± 0.156
0.657PheIle: 0.657 ± 0.179
1.032PheLys: 1.032 ± 0.217
1.548PheLeu: 1.548 ± 0.302
0.281PheMet: 0.281 ± 0.117
0.891PheAsn: 0.891 ± 0.198
1.313PhePro: 1.313 ± 0.238
0.563PheGln: 0.563 ± 0.158
1.548PheArg: 1.548 ± 0.257
1.173PheSer: 1.173 ± 0.246
1.548PheThr: 1.548 ± 0.225
1.36PheVal: 1.36 ± 0.192
0.375PheTrp: 0.375 ± 0.139
0.704PheTyr: 0.704 ± 0.196
0.0PheXaa: 0.0 ± 0.0
Gly
9.381GlyAla: 9.381 ± 1.537
1.126GlyCys: 1.126 ± 0.267
5.394GlyAsp: 5.394 ± 0.618
5.347GlyGlu: 5.347 ± 0.551
2.064GlyPhe: 2.064 ± 0.333
13.087GlyGly: 13.087 ± 2.79
1.829GlyHis: 1.829 ± 0.341
4.878GlyIle: 4.878 ± 0.37
3.518GlyLys: 3.518 ± 0.464
7.927GlyLeu: 7.927 ± 0.902
2.158GlyMet: 2.158 ± 0.329
2.486GlyAsn: 2.486 ± 0.513
4.644GlyPro: 4.644 ± 0.496
3.706GlyGln: 3.706 ± 0.497
5.77GlyArg: 5.77 ± 0.588
5.254GlySer: 5.254 ± 0.549
6.801GlyThr: 6.801 ± 0.625
6.895GlyVal: 6.895 ± 0.533
1.782GlyTrp: 1.782 ± 0.281
2.767GlyTyr: 2.767 ± 0.335
0.0GlyXaa: 0.0 ± 0.0
His
1.876HisAla: 1.876 ± 0.316
0.328HisCys: 0.328 ± 0.139
1.079HisAsp: 1.079 ± 0.245
1.501HisGlu: 1.501 ± 0.313
0.422HisPhe: 0.422 ± 0.118
1.501HisGly: 1.501 ± 0.227
0.891HisHis: 0.891 ± 0.351
1.407HisIle: 1.407 ± 0.292
0.422HisLys: 0.422 ± 0.165
1.36HisLeu: 1.36 ± 0.268
0.563HisMet: 0.563 ± 0.148
0.375HisAsn: 0.375 ± 0.115
1.173HisPro: 1.173 ± 0.288
0.281HisGln: 0.281 ± 0.101
2.158HisArg: 2.158 ± 0.351
0.891HisSer: 0.891 ± 0.193
2.064HisThr: 2.064 ± 0.362
1.079HisVal: 1.079 ± 0.243
0.188HisTrp: 0.188 ± 0.096
0.563HisTyr: 0.563 ± 0.157
0.0HisXaa: 0.0 ± 0.0
Ile
5.676IleAla: 5.676 ± 0.498
0.61IleCys: 0.61 ± 0.2
3.846IleAsp: 3.846 ± 0.4
4.831IleGlu: 4.831 ± 0.484
0.938IlePhe: 0.938 ± 0.208
5.441IleGly: 5.441 ± 0.554
0.61IleHis: 0.61 ± 0.198
1.642IleIle: 1.642 ± 0.302
1.22IleLys: 1.22 ± 0.333
2.721IleLeu: 2.721 ± 0.359
0.797IleMet: 0.797 ± 0.188
1.501IleAsn: 1.501 ± 0.253
2.721IlePro: 2.721 ± 0.333
0.985IleGln: 0.985 ± 0.235
2.392IleArg: 2.392 ± 0.371
2.298IleSer: 2.298 ± 0.368
3.471IleThr: 3.471 ± 0.309
3.19IleVal: 3.19 ± 0.392
0.375IleTrp: 0.375 ± 0.134
0.938IleTyr: 0.938 ± 0.196
0.0IleXaa: 0.0 ± 0.0
Lys
3.987LysAla: 3.987 ± 0.525
0.094LysCys: 0.094 ± 0.07
1.501LysAsp: 1.501 ± 0.216
0.797LysGlu: 0.797 ± 0.262
0.563LysPhe: 0.563 ± 0.156
2.627LysGly: 2.627 ± 0.302
0.516LysHis: 0.516 ± 0.177
1.782LysIle: 1.782 ± 0.296
0.891LysLys: 0.891 ± 0.227
2.861LysLeu: 2.861 ± 0.379
0.422LysMet: 0.422 ± 0.149
0.797LysAsn: 0.797 ± 0.194
1.454LysPro: 1.454 ± 0.287
1.407LysGln: 1.407 ± 0.238
2.158LysArg: 2.158 ± 0.339
1.36LysSer: 1.36 ± 0.252
2.345LysThr: 2.345 ± 0.322
2.627LysVal: 2.627 ± 0.351
0.328LysTrp: 0.328 ± 0.113
0.704LysTyr: 0.704 ± 0.159
0.0LysXaa: 0.0 ± 0.0
Leu
11.304LeuAla: 11.304 ± 0.868
1.126LeuCys: 1.126 ± 0.255
6.285LeuAsp: 6.285 ± 0.565
3.237LeuGlu: 3.237 ± 0.328
1.876LeuPhe: 1.876 ± 0.361
7.364LeuGly: 7.364 ± 1.014
1.032LeuHis: 1.032 ± 0.219
4.034LeuIle: 4.034 ± 0.491
2.392LeuLys: 2.392 ± 0.39
5.207LeuLeu: 5.207 ± 0.526
1.548LeuMet: 1.548 ± 0.256
3.237LeuAsn: 3.237 ± 0.598
5.957LeuPro: 5.957 ± 0.512
1.595LeuGln: 1.595 ± 0.27
4.128LeuArg: 4.128 ± 0.552
3.987LeuSer: 3.987 ± 0.347
7.13LeuThr: 7.13 ± 0.553
4.784LeuVal: 4.784 ± 0.486
1.266LeuTrp: 1.266 ± 0.228
1.642LeuTyr: 1.642 ± 0.312
0.0LeuXaa: 0.0 ± 0.0
Met
2.392MetAla: 2.392 ± 0.319
0.235MetCys: 0.235 ± 0.103
0.657MetAsp: 0.657 ± 0.17
0.704MetGlu: 0.704 ± 0.165
0.751MetPhe: 0.751 ± 0.188
1.501MetGly: 1.501 ± 0.226
0.422MetHis: 0.422 ± 0.179
0.891MetIle: 0.891 ± 0.171
0.704MetLys: 0.704 ± 0.186
1.407MetLeu: 1.407 ± 0.229
0.328MetMet: 0.328 ± 0.111
0.469MetAsn: 0.469 ± 0.157
1.548MetPro: 1.548 ± 0.268
0.375MetGln: 0.375 ± 0.143
1.266MetArg: 1.266 ± 0.184
1.923MetSer: 1.923 ± 0.298
2.767MetThr: 2.767 ± 0.349
1.407MetVal: 1.407 ± 0.257
0.516MetTrp: 0.516 ± 0.157
0.516MetTyr: 0.516 ± 0.186
0.0MetXaa: 0.0 ± 0.0
Asn
3.612AsnAla: 3.612 ± 0.382
0.235AsnCys: 0.235 ± 0.091
2.017AsnAsp: 2.017 ± 0.35
1.266AsnGlu: 1.266 ± 0.251
0.328AsnPhe: 0.328 ± 0.113
3.471AsnGly: 3.471 ± 0.474
0.563AsnHis: 0.563 ± 0.164
1.36AsnIle: 1.36 ± 0.296
1.032AsnLys: 1.032 ± 0.239
1.782AsnLeu: 1.782 ± 0.287
0.469AsnMet: 0.469 ± 0.102
1.266AsnAsn: 1.266 ± 0.296
1.782AsnPro: 1.782 ± 0.286
0.375AsnGln: 0.375 ± 0.117
2.017AsnArg: 2.017 ± 0.356
1.642AsnSer: 1.642 ± 0.319
2.627AsnThr: 2.627 ± 0.388
1.876AsnVal: 1.876 ± 0.282
0.422AsnTrp: 0.422 ± 0.155
0.891AsnTyr: 0.891 ± 0.209
0.0AsnXaa: 0.0 ± 0.0
Pro
7.505ProAla: 7.505 ± 0.595
0.422ProCys: 0.422 ± 0.157
4.222ProAsp: 4.222 ± 0.534
5.441ProGlu: 5.441 ± 0.791
1.22ProPhe: 1.22 ± 0.22
6.098ProGly: 6.098 ± 0.642
1.266ProHis: 1.266 ± 0.291
2.721ProIle: 2.721 ± 0.398
1.266ProLys: 1.266 ± 0.258
3.377ProLeu: 3.377 ± 0.396
1.266ProMet: 1.266 ± 0.214
2.111ProAsn: 2.111 ± 0.323
4.691ProPro: 4.691 ± 0.705
1.782ProGln: 1.782 ± 0.242
3.893ProArg: 3.893 ± 0.55
2.627ProSer: 2.627 ± 0.411
3.846ProThr: 3.846 ± 0.377
5.16ProVal: 5.16 ± 0.525
1.313ProTrp: 1.313 ± 0.239
1.032ProTyr: 1.032 ± 0.234
0.0ProXaa: 0.0 ± 0.0
Gln
4.081GlnAla: 4.081 ± 0.727
0.235GlnCys: 0.235 ± 0.127
1.126GlnAsp: 1.126 ± 0.25
0.891GlnGlu: 0.891 ± 0.194
1.126GlnPhe: 1.126 ± 0.198
2.205GlnGly: 2.205 ± 0.323
0.938GlnHis: 0.938 ± 0.196
2.064GlnIle: 2.064 ± 0.262
0.844GlnLys: 0.844 ± 0.219
2.439GlnLeu: 2.439 ± 0.29
0.657GlnMet: 0.657 ± 0.159
0.751GlnAsn: 0.751 ± 0.207
1.782GlnPro: 1.782 ± 0.269
1.642GlnGln: 1.642 ± 0.269
3.049GlnArg: 3.049 ± 0.402
1.642GlnSer: 1.642 ± 0.296
2.158GlnThr: 2.158 ± 0.316
1.97GlnVal: 1.97 ± 0.273
0.751GlnTrp: 0.751 ± 0.2
0.657GlnTyr: 0.657 ± 0.213
0.0GlnXaa: 0.0 ± 0.0
Arg
7.13ArgAla: 7.13 ± 0.605
0.657ArgCys: 0.657 ± 0.193
4.456ArgAsp: 4.456 ± 0.58
4.784ArgGlu: 4.784 ± 0.594
1.501ArgPhe: 1.501 ± 0.273
4.362ArgGly: 4.362 ± 0.468
1.736ArgHis: 1.736 ± 0.335
2.252ArgIle: 2.252 ± 0.31
1.97ArgLys: 1.97 ± 0.296
6.004ArgLeu: 6.004 ± 0.485
1.595ArgMet: 1.595 ± 0.382
2.486ArgAsn: 2.486 ± 0.4
3.94ArgPro: 3.94 ± 0.528
2.533ArgGln: 2.533 ± 0.373
6.379ArgArg: 6.379 ± 0.672
2.767ArgSer: 2.767 ± 0.307
3.987ArgThr: 3.987 ± 0.431
5.582ArgVal: 5.582 ± 0.612
1.736ArgTrp: 1.736 ± 0.287
2.205ArgTyr: 2.205 ± 0.395
0.0ArgXaa: 0.0 ± 0.0
Ser
5.019SerAla: 5.019 ± 0.497
0.657SerCys: 0.657 ± 0.201
3.283SerAsp: 3.283 ± 0.288
2.252SerGlu: 2.252 ± 0.351
0.797SerPhe: 0.797 ± 0.207
5.816SerGly: 5.816 ± 0.711
0.751SerHis: 0.751 ± 0.205
1.736SerIle: 1.736 ± 0.337
1.126SerLys: 1.126 ± 0.186
4.081SerLeu: 4.081 ± 0.677
1.266SerMet: 1.266 ± 0.276
1.079SerAsn: 1.079 ± 0.229
2.908SerPro: 2.908 ± 0.38
2.017SerGln: 2.017 ± 0.259
3.471SerArg: 3.471 ± 0.308
2.439SerSer: 2.439 ± 0.398
4.268SerThr: 4.268 ± 0.593
3.706SerVal: 3.706 ± 0.44
0.985SerTrp: 0.985 ± 0.208
1.501SerTyr: 1.501 ± 0.224
0.0SerXaa: 0.0 ± 0.0
Thr
9.194ThrAla: 9.194 ± 0.565
0.469ThrCys: 0.469 ± 0.151
5.254ThrAsp: 5.254 ± 0.425
3.659ThrGlu: 3.659 ± 0.39
1.642ThrPhe: 1.642 ± 0.243
7.224ThrGly: 7.224 ± 0.518
1.032ThrHis: 1.032 ± 0.257
3.518ThrIle: 3.518 ± 0.469
2.58ThrLys: 2.58 ± 0.299
5.16ThrLeu: 5.16 ± 0.49
1.689ThrMet: 1.689 ± 0.261
2.111ThrAsn: 2.111 ± 0.381
5.113ThrPro: 5.113 ± 0.626
1.97ThrGln: 1.97 ± 0.311
3.706ThrArg: 3.706 ± 0.445
4.222ThrSer: 4.222 ± 0.38
3.94ThrThr: 3.94 ± 0.538
5.77ThrVal: 5.77 ± 0.53
1.22ThrTrp: 1.22 ± 0.268
1.501ThrTyr: 1.501 ± 0.274
0.0ThrXaa: 0.0 ± 0.0
Val
8.443ValAla: 8.443 ± 0.483
0.938ValCys: 0.938 ± 0.243
4.362ValAsp: 4.362 ± 0.503
5.957ValGlu: 5.957 ± 0.576
1.501ValPhe: 1.501 ± 0.22
7.458ValGly: 7.458 ± 0.56
1.689ValHis: 1.689 ± 0.283
3.377ValIle: 3.377 ± 0.346
1.97ValLys: 1.97 ± 0.314
6.473ValLeu: 6.473 ± 0.465
1.407ValMet: 1.407 ± 0.309
2.064ValAsn: 2.064 ± 0.28
3.893ValPro: 3.893 ± 0.354
1.829ValGln: 1.829 ± 0.278
4.644ValArg: 4.644 ± 0.547
4.034ValSer: 4.034 ± 0.401
5.207ValThr: 5.207 ± 0.579
6.004ValVal: 6.004 ± 0.717
1.829ValTrp: 1.829 ± 0.332
1.923ValTyr: 1.923 ± 0.407
0.0ValXaa: 0.0 ± 0.0
Trp
2.298TrpAla: 2.298 ± 0.357
0.422TrpCys: 0.422 ± 0.15
1.22TrpAsp: 1.22 ± 0.247
0.516TrpGlu: 0.516 ± 0.165
0.657TrpPhe: 0.657 ± 0.204
0.938TrpGly: 0.938 ± 0.202
0.516TrpHis: 0.516 ± 0.176
0.938TrpIle: 0.938 ± 0.199
0.281TrpLys: 0.281 ± 0.104
1.501TrpLeu: 1.501 ± 0.261
0.516TrpMet: 0.516 ± 0.163
0.281TrpAsn: 0.281 ± 0.093
0.891TrpPro: 0.891 ± 0.247
0.657TrpGln: 0.657 ± 0.183
1.36TrpArg: 1.36 ± 0.237
1.313TrpSer: 1.313 ± 0.227
1.548TrpThr: 1.548 ± 0.334
1.501TrpVal: 1.501 ± 0.336
0.422TrpTrp: 0.422 ± 0.153
0.422TrpTyr: 0.422 ± 0.163
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.33TyrAla: 3.33 ± 0.361
0.281TyrCys: 0.281 ± 0.117
2.017TyrAsp: 2.017 ± 0.265
1.501TyrGlu: 1.501 ± 0.28
0.61TyrPhe: 0.61 ± 0.169
2.205TyrGly: 2.205 ± 0.39
0.469TyrHis: 0.469 ± 0.133
1.126TyrIle: 1.126 ± 0.213
0.563TyrLys: 0.563 ± 0.175
1.923TyrLeu: 1.923 ± 0.371
0.328TyrMet: 0.328 ± 0.128
0.704TyrAsn: 0.704 ± 0.163
1.079TyrPro: 1.079 ± 0.259
0.844TyrGln: 0.844 ± 0.228
2.111TyrArg: 2.111 ± 0.338
1.032TyrSer: 1.032 ± 0.175
1.595TyrThr: 1.595 ± 0.25
2.252TyrVal: 2.252 ± 0.262
0.235TyrTrp: 0.235 ± 0.113
0.469TyrTyr: 0.469 ± 0.165
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 99 proteins (21320 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski