Digitaria ciliaris striate mosaic virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Mastrevirus

Average proteome isoelectric point is 7.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J7FHK0|J7FHK0_9GEMI Replication-associated protein OS=Digitaria ciliaris striate mosaic virus OX=1196237 PE=3 SV=1
MM1 pKa = 7.75APPVSDD7 pKa = 3.61TEE9 pKa = 4.46SANSANCRR17 pKa = 11.84LAEE20 pKa = 4.19RR21 pKa = 11.84APGAAEE27 pKa = 3.59ASFEE31 pKa = 4.05VRR33 pKa = 11.84AKK35 pKa = 10.93NIFLTYY41 pKa = 10.09SKK43 pKa = 10.79CLLDD47 pKa = 3.8PQEE50 pKa = 4.19ALRR53 pKa = 11.84DD54 pKa = 3.65ITHH57 pKa = 7.74KK58 pKa = 10.51LRR60 pKa = 11.84KK61 pKa = 9.4FEE63 pKa = 3.88PTYY66 pKa = 10.88VYY68 pKa = 10.34VARR71 pKa = 11.84EE72 pKa = 3.88LHH74 pKa = 5.95QDD76 pKa = 3.28GTFHH80 pKa = 6.66LHH82 pKa = 7.11CFVQCKK88 pKa = 8.88KK89 pKa = 10.12HH90 pKa = 5.51VRR92 pKa = 11.84TTRR95 pKa = 11.84ARR97 pKa = 11.84FFDD100 pKa = 3.72LEE102 pKa = 4.37EE103 pKa = 3.9YY104 pKa = 10.2HH105 pKa = 7.46PNVQNARR112 pKa = 11.84MPHH115 pKa = 6.32KK116 pKa = 10.75VLAYY120 pKa = 9.6CKK122 pKa = 9.77KK123 pKa = 10.5SPVSYY128 pKa = 10.65AEE130 pKa = 3.76EE131 pKa = 4.14GAYY134 pKa = 9.31TEE136 pKa = 3.92SDD138 pKa = 3.27VRR140 pKa = 11.84KK141 pKa = 9.91RR142 pKa = 11.84KK143 pKa = 9.36IDD145 pKa = 3.33ASTTKK150 pKa = 9.81DD151 pKa = 2.98AKK153 pKa = 9.69MADD156 pKa = 3.92IIRR159 pKa = 11.84SSKK162 pKa = 10.8SKK164 pKa = 10.91EE165 pKa = 4.03EE166 pKa = 3.9YY167 pKa = 10.35LSMVRR172 pKa = 11.84KK173 pKa = 7.79TFPFDD178 pKa = 2.69WATRR182 pKa = 11.84LQNFEE187 pKa = 4.21YY188 pKa = 10.33SAEE191 pKa = 4.05RR192 pKa = 11.84LFPSTPPPYY201 pKa = 10.17VSPFNMPSQEE211 pKa = 3.93EE212 pKa = 4.54HH213 pKa = 6.56PVLGAWLRR221 pKa = 11.84AEE223 pKa = 5.16LYY225 pKa = 8.99TVSPYY230 pKa = 10.83AYY232 pKa = 8.99SIHH235 pKa = 6.72SGLSEE240 pKa = 3.87EE241 pKa = 4.42QARR244 pKa = 11.84ADD246 pKa = 4.19LQWMHH251 pKa = 6.47DD252 pKa = 3.53TSRR255 pKa = 11.84AEE257 pKa = 3.83TLLNEE262 pKa = 4.49EE263 pKa = 4.64NPSTSADD270 pKa = 3.31QLVPEE275 pKa = 5.2RR276 pKa = 11.84PPGPEE281 pKa = 3.13VWASITTGNTASTSSTSFPTLNTMM305 pKa = 4.9

Molecular weight: 34.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J7FGV8|J7FGV8_9GEMI Replication-associated protein OS=Digitaria ciliaris striate mosaic virus OX=1196237 PE=3 SV=1
MM1 pKa = 7.43PASSKK6 pKa = 10.36RR7 pKa = 11.84KK8 pKa = 9.34RR9 pKa = 11.84GSSSAGKK16 pKa = 9.46RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 9.5KK20 pKa = 10.11PRR22 pKa = 11.84YY23 pKa = 7.05TKK25 pKa = 8.25WTGSRR30 pKa = 11.84SSASQDD36 pKa = 2.87ALQVQTFQYY45 pKa = 11.29AEE47 pKa = 4.01DD48 pKa = 3.55QAFNAGGRR56 pKa = 11.84ALLLTAFTRR65 pKa = 11.84GSAEE69 pKa = 3.99NQRR72 pKa = 11.84KK73 pKa = 7.88SQEE76 pKa = 4.03TITYY80 pKa = 8.49KK81 pKa = 10.63VSVSLGVSASSTVQKK96 pKa = 10.85YY97 pKa = 8.74CVKK100 pKa = 10.27SQPICWLVYY109 pKa = 10.45DD110 pKa = 4.47KK111 pKa = 10.93TPTGIADD118 pKa = 4.75LVPSDD123 pKa = 3.92IFDD126 pKa = 3.78VPSGLTNWPSTWKK139 pKa = 10.14VKK141 pKa = 10.67RR142 pKa = 11.84EE143 pKa = 3.68VSHH146 pKa = 6.58RR147 pKa = 11.84FVVKK151 pKa = 10.34RR152 pKa = 11.84RR153 pKa = 11.84WPFTLSCNGSTFTADD168 pKa = 3.43YY169 pKa = 9.01TKK171 pKa = 11.02LPVPNTDD178 pKa = 2.95NLVSVNRR185 pKa = 11.84FAKK188 pKa = 9.97GLGVRR193 pKa = 11.84TEE195 pKa = 3.9WKK197 pKa = 9.03DD198 pKa = 3.58TVSAEE203 pKa = 3.9ASDD206 pKa = 3.63IKK208 pKa = 11.09GGALYY213 pKa = 10.13IVLAPANGVVFTARR227 pKa = 11.84GVIKK231 pKa = 10.61VYY233 pKa = 10.27FKK235 pKa = 11.22SVGNQQ240 pKa = 2.8

Molecular weight: 26.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1022

113

364

255.5

28.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.295 ± 0.359

1.859 ± 0.261

4.403 ± 0.229

5.773 ± 1.176

4.599 ± 0.203

5.284 ± 1.33

2.153 ± 0.614

2.935 ± 0.292

6.067 ± 1.007

6.947 ± 0.397

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.566 ± 0.354

3.718 ± 0.353

6.262 ± 0.536

3.718 ± 0.246

6.458 ± 0.082

9.002 ± 1.107

6.947 ± 0.615

7.241 ± 1.15

1.761 ± 0.269

4.012 ± 0.32
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski