Gonapodya prolifera (strain JEL478) (Monoblepharis prolifera)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Chytridiomycota; Chytridiomycota incertae sedis; Monoblepharidomycetes; Monoblepharidales; Gonapodyaceae; Gonapodya; Gonapodya prolifera

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13801 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A139A1Q6|A0A139A1Q6_GONPJ t-SNARE OS=Gonapodya prolifera (strain JEL478) OX=1344416 GN=M427DRAFT_158862 PE=3 SV=1
MM1 pKa = 7.26PRR3 pKa = 11.84SRR5 pKa = 11.84EE6 pKa = 3.57EE7 pKa = 3.77ALRR10 pKa = 11.84MEE12 pKa = 4.8DD13 pKa = 3.85EE14 pKa = 4.33IEE16 pKa = 4.05EE17 pKa = 4.35GQYY20 pKa = 10.97EE21 pKa = 4.29PGEE24 pKa = 3.96LLEE27 pKa = 5.21IEE29 pKa = 4.42GDD31 pKa = 3.67GDD33 pKa = 3.61GLEE36 pKa = 4.14NVEE39 pKa = 4.97ASVDD43 pKa = 3.78DD44 pKa = 4.7LEE46 pKa = 5.05PPSTAPYY53 pKa = 10.27LGGEE57 pKa = 4.27GHH59 pKa = 6.93CARR62 pKa = 11.84GNGTDD67 pKa = 4.12NGWCVDD73 pKa = 3.76TQAAAVDD80 pKa = 4.08GSYY83 pKa = 11.08RR84 pKa = 11.84DD85 pKa = 3.24IALAALRR92 pKa = 11.84AVGMASLPTTIPSTVLNAQPASSHH116 pKa = 5.52FRR118 pKa = 11.84SPPSVPTCDD127 pKa = 3.32TQGRR131 pKa = 11.84PVWNGPDD138 pKa = 3.15WSTLTGLDD146 pKa = 3.85PYY148 pKa = 10.54GGPWVTMSEE157 pKa = 4.21PQVPPAGDD165 pKa = 3.12VDD167 pKa = 4.45GPSLQVDD174 pKa = 4.36DD175 pKa = 6.24DD176 pKa = 4.82IVWDD180 pKa = 3.76WAGCDD185 pKa = 3.45QSGEE189 pKa = 4.12IASALCWVTPMLRR202 pKa = 11.84GCDD205 pKa = 3.48LL206 pKa = 3.88

Molecular weight: 21.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A139AQD2|A0A139AQD2_GONPJ Peptidylprolyl isomerase OS=Gonapodya prolifera (strain JEL478) OX=1344416 GN=M427DRAFT_53658 PE=4 SV=1
MM1 pKa = 6.82YY2 pKa = 9.11TSQHH6 pKa = 6.43LLPLSLSQHH15 pKa = 6.04VSTQPPTTSSPPSALMPRR33 pKa = 11.84PAPRR37 pKa = 11.84TLASAATPATLPRR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84FTRR57 pKa = 11.84PTAASFFRR65 pKa = 11.84TRR67 pKa = 11.84RR68 pKa = 11.84GLL70 pKa = 3.45

Molecular weight: 7.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13797

4

13801

5698899

49

5347

412.9

45.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.281 ± 0.023

1.231 ± 0.009

5.42 ± 0.015

5.84 ± 0.025

3.545 ± 0.014

7.458 ± 0.021

2.346 ± 0.011

4.229 ± 0.017

4.373 ± 0.019

8.976 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.042 ± 0.008

3.211 ± 0.012

6.618 ± 0.031

3.42 ± 0.015

6.488 ± 0.021

8.51 ± 0.024

6.148 ± 0.019

7.085 ± 0.016

1.433 ± 0.008

2.311 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski