Wenzhou tombus-like virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KH30|A0A1L3KH30_9VIRU RNA-directed RNA polymerase OS=Wenzhou tombus-like virus 2 OX=1923672 PE=4 SV=1
MM1 pKa = 7.49RR2 pKa = 11.84RR3 pKa = 11.84AITEE7 pKa = 3.59RR8 pKa = 11.84VLYY11 pKa = 9.93VRR13 pKa = 11.84RR14 pKa = 11.84DD15 pKa = 3.33GRR17 pKa = 11.84LTRR20 pKa = 11.84PPRR23 pKa = 11.84PEE25 pKa = 3.89PGFYY29 pKa = 10.59GQLSGIRR36 pKa = 11.84QRR38 pKa = 11.84LVRR41 pKa = 11.84KK42 pKa = 7.57ATPTPVVPLAEE53 pKa = 4.35YY54 pKa = 9.52PSLYY58 pKa = 9.99HH59 pKa = 5.52GRR61 pKa = 11.84KK62 pKa = 8.95RR63 pKa = 11.84KK64 pKa = 9.71IYY66 pKa = 8.43EE67 pKa = 3.83NAVNSLNEE75 pKa = 4.06RR76 pKa = 11.84GLTAKK81 pKa = 10.2DD82 pKa = 2.9AWVDD86 pKa = 3.55TFLKK90 pKa = 10.59AEE92 pKa = 4.27KK93 pKa = 10.88VNFDD97 pKa = 4.05SKK99 pKa = 11.44GDD101 pKa = 3.73PAPRR105 pKa = 11.84LISPRR110 pKa = 11.84GPRR113 pKa = 11.84YY114 pKa = 8.49NVEE117 pKa = 3.52VGRR120 pKa = 11.84YY121 pKa = 8.45LKK123 pKa = 10.82LFEE126 pKa = 5.2KK127 pKa = 10.61EE128 pKa = 3.64LFRR131 pKa = 11.84AFEE134 pKa = 4.0RR135 pKa = 11.84VFGYY139 pKa = 10.39KK140 pKa = 9.96VVVKK144 pKa = 10.1GLNAQQVGEE153 pKa = 4.84LLAQHH158 pKa = 6.31WGEE161 pKa = 3.75FRR163 pKa = 11.84EE164 pKa = 4.39PVAVGLDD171 pKa = 3.21ASRR174 pKa = 11.84FDD176 pKa = 3.42QHH178 pKa = 6.28VSRR181 pKa = 11.84DD182 pKa = 3.63ALEE185 pKa = 4.6WEE187 pKa = 4.14HH188 pKa = 7.38SVYY191 pKa = 10.89NAVFRR196 pKa = 11.84SEE198 pKa = 3.98RR199 pKa = 11.84LRR201 pKa = 11.84WLLRR205 pKa = 11.84MQLRR209 pKa = 11.84NRR211 pKa = 11.84CIARR215 pKa = 11.84VEE217 pKa = 4.08GRR219 pKa = 11.84RR220 pKa = 11.84FDD222 pKa = 3.62YY223 pKa = 8.44TTDD226 pKa = 3.39GVRR229 pKa = 11.84MSGDD233 pKa = 3.65LNTSLGNCLIMSSLVLRR250 pKa = 11.84YY251 pKa = 9.99LEE253 pKa = 4.02EE254 pKa = 5.4RR255 pKa = 11.84GVKK258 pKa = 9.9ARR260 pKa = 11.84LANNGDD266 pKa = 3.77DD267 pKa = 3.72CVVFCEE273 pKa = 4.82AADD276 pKa = 3.99LSKK279 pKa = 11.19LDD281 pKa = 5.28GLDD284 pKa = 3.13HH285 pKa = 6.48WFLRR289 pKa = 11.84AGFTLTRR296 pKa = 11.84EE297 pKa = 4.33DD298 pKa = 3.11PCYY301 pKa = 10.3EE302 pKa = 3.92LEE304 pKa = 4.4QVEE307 pKa = 5.11FCQFHH312 pKa = 6.34PVEE315 pKa = 4.82CANGWRR321 pKa = 11.84MVRR324 pKa = 11.84DD325 pKa = 3.42PRR327 pKa = 11.84VAMSKK332 pKa = 10.47DD333 pKa = 3.49CVSVVGWDD341 pKa = 3.34QEE343 pKa = 4.8SEE345 pKa = 4.55VKK347 pKa = 10.19VWAAAVGQCGLSLTAGVPVWEE368 pKa = 4.19SWYY371 pKa = 10.42KK372 pKa = 9.52RR373 pKa = 11.84LVRR376 pKa = 11.84VGVEE380 pKa = 3.63RR381 pKa = 11.84DD382 pKa = 3.18SGVGEE387 pKa = 4.07RR388 pKa = 11.84VKK390 pKa = 10.85EE391 pKa = 3.97CGMAYY396 pKa = 10.2AAAGVQACPISDD408 pKa = 3.4ACRR411 pKa = 11.84YY412 pKa = 9.3SFYY415 pKa = 10.45RR416 pKa = 11.84AFGILPDD423 pKa = 3.73LQEE426 pKa = 4.17ALEE429 pKa = 4.34RR430 pKa = 11.84EE431 pKa = 4.26YY432 pKa = 11.44SADD435 pKa = 3.49YY436 pKa = 10.02TLAPIDD442 pKa = 4.18PMMSSHH448 pKa = 7.14IKK450 pKa = 9.89TIDD453 pKa = 3.24QLNPLTLHH461 pKa = 6.39NGSS464 pKa = 3.9

Molecular weight: 52.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KH30|A0A1L3KH30_9VIRU RNA-directed RNA polymerase OS=Wenzhou tombus-like virus 2 OX=1923672 PE=4 SV=1
MM1 pKa = 7.12ARR3 pKa = 11.84KK4 pKa = 9.04RR5 pKa = 11.84AKK7 pKa = 8.87QAQMPVSGGTRR18 pKa = 11.84RR19 pKa = 11.84NKK21 pKa = 10.61GYY23 pKa = 8.69TPTIKK28 pKa = 10.04GTGDD32 pKa = 3.3STIIGYY38 pKa = 9.41NGQLQPLIVNTSGRR52 pKa = 11.84GLNFRR57 pKa = 11.84QYY59 pKa = 10.69IGGFQEE65 pKa = 4.69AFAPGAIGPTIVARR79 pKa = 11.84YY80 pKa = 8.63ASCKK84 pKa = 9.63FLPGTTLRR92 pKa = 11.84WEE94 pKa = 4.23PSVSFSTSGRR104 pKa = 11.84VYY106 pKa = 11.03VGFTDD111 pKa = 3.62NPEE114 pKa = 3.61VTAYY118 pKa = 9.2MINLAVLTAADD129 pKa = 3.88PTNTGKK135 pKa = 9.81WNNYY139 pKa = 6.82EE140 pKa = 4.09SAVRR144 pKa = 11.84ALGSCISFPVWQEE157 pKa = 3.13TDD159 pKa = 2.83IPFPTKK165 pKa = 10.31LRR167 pKa = 11.84RR168 pKa = 11.84KK169 pKa = 10.07RR170 pKa = 11.84FDD172 pKa = 3.4TNQVFPNIAPAIDD185 pKa = 3.55TLDD188 pKa = 3.7RR189 pKa = 11.84CCQTTMFAAVVGVNTAVALTVGNFHH214 pKa = 6.39VHH216 pKa = 6.96DD217 pKa = 4.34KK218 pKa = 11.16VDD220 pKa = 3.6VEE222 pKa = 4.86GITPFITDD230 pKa = 3.11IDD232 pKa = 4.07TSLVASATPGSSSVQNLGHH251 pKa = 6.27SGGDD255 pKa = 3.11AVVGLGRR262 pKa = 11.84PHH264 pKa = 7.12PSAIAGYY271 pKa = 9.55NRR273 pKa = 11.84PLL275 pKa = 4.0

Molecular weight: 29.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

739

275

464

369.5

41.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.39 ± 0.408

2.165 ± 0.413

5.142 ± 0.453

5.413 ± 1.878

4.195 ± 0.31

8.39 ± 1.042

1.624 ± 0.098

3.654 ± 1.258

3.654 ± 0.221

8.119 ± 1.549

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.759 ± 0.177

4.06 ± 0.811

5.548 ± 0.791

3.248 ± 0.226

8.66 ± 1.652

5.954 ± 0.344

5.683 ± 2.404

9.066 ± 0.409

1.759 ± 0.388

3.518 ± 0.354
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski