Vibrio phage Va_PF430-3_p42

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A513SPJ1|A0A513SPJ1_9CAUD Threonine--tRNA ligase OS=Vibrio phage Va_PF430-3_p42 OX=2565524 PE=3 SV=1
MM1 pKa = 7.79PLHH4 pKa = 5.91TNEE7 pKa = 4.62MPFDD11 pKa = 3.81HH12 pKa = 7.09LGGYY16 pKa = 9.03LQVEE20 pKa = 4.86VVGSGTAQLQVNSGEE35 pKa = 3.91NWVNCGEE42 pKa = 4.1PLSEE46 pKa = 5.46GVYY49 pKa = 10.76CDD51 pKa = 3.51LFKK54 pKa = 10.56PAVYY58 pKa = 10.19RR59 pKa = 11.84FSLTDD64 pKa = 2.99ATAFTRR70 pKa = 4.06

Molecular weight: 7.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A513SPI1|A0A513SPI1_9CAUD 50S ribosomal protein L20 OS=Vibrio phage Va_PF430-3_p42 OX=2565524 PE=3 SV=1
MM1 pKa = 7.82KK2 pKa = 10.46DD3 pKa = 3.15GGVLFALITCYY14 pKa = 10.75LNYY17 pKa = 10.73YY18 pKa = 8.89NAEE21 pKa = 4.09LFIMPKK27 pKa = 9.48MKK29 pKa = 9.9TNKK32 pKa = 9.6GAAKK36 pKa = 10.06RR37 pKa = 11.84FKK39 pKa = 9.65KK40 pKa = 8.71TAGGIKK46 pKa = 9.87FKK48 pKa = 10.64HH49 pKa = 4.52ATKK52 pKa = 10.47RR53 pKa = 11.84HH54 pKa = 5.38ILTKK58 pKa = 9.93RR59 pKa = 11.84TTKK62 pKa = 10.35NKK64 pKa = 9.9RR65 pKa = 11.84QLRR68 pKa = 11.84PNSILPKK75 pKa = 10.17CEE77 pKa = 3.6VAGVMRR83 pKa = 11.84MLPYY87 pKa = 10.82AA88 pKa = 4.17

Molecular weight: 10.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

16145

67

916

252.3

28.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.523 ± 0.359

1.239 ± 0.127

5.68 ± 0.22

6.894 ± 0.241

3.908 ± 0.192

6.72 ± 0.339

2.094 ± 0.162

5.909 ± 0.186

6.658 ± 0.244

8.684 ± 0.261

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.663 ± 0.11

4.652 ± 0.218

3.976 ± 0.223

4.076 ± 0.186

5.81 ± 0.303

6.219 ± 0.288

5.55 ± 0.167

6.454 ± 0.224

1.35 ± 0.118

2.942 ± 0.213
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski