Clostridium phage phiCP26F

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F2VHU2|F2VHU2_9CAUD Uncharacterized protein phi26F_gp34 OS=Clostridium phage phiCP26F OX=673376 GN=phi26F_gp34 PE=4 SV=1
MM1 pKa = 7.39SVNEE5 pKa = 3.97LLEE8 pKa = 4.31VVSQGQRR15 pKa = 11.84IAFCYY20 pKa = 9.44DD21 pKa = 3.06CCYY24 pKa = 10.6KK25 pKa = 10.64PLVWEE30 pKa = 4.4EE31 pKa = 3.87DD32 pKa = 3.04MSYY35 pKa = 11.67DD36 pKa = 3.59DD37 pKa = 5.44FIYY40 pKa = 10.38GVVPFKK46 pKa = 10.97EE47 pKa = 4.26KK48 pKa = 10.99AFLDD52 pKa = 3.97SEE54 pKa = 4.66VLFIYY59 pKa = 9.67TNNDD63 pKa = 2.4NTLWVDD69 pKa = 3.39VEE71 pKa = 4.51VFF73 pKa = 3.2

Molecular weight: 8.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F2VHW5|F2VHW5_9CAUD Uncharacterized protein phi26F_gp8 OS=Clostridium phage phiCP26F OX=673376 GN=phi26F_gp8 PE=4 SV=1
MM1 pKa = 7.55GRR3 pKa = 11.84FICEE7 pKa = 4.71AIPYY11 pKa = 9.62NGFKK15 pKa = 10.56EE16 pKa = 4.04RR17 pKa = 11.84IKK19 pKa = 10.31ICKK22 pKa = 8.44EE23 pKa = 3.69LQRR26 pKa = 11.84KK27 pKa = 8.45RR28 pKa = 11.84MRR30 pKa = 11.84FSIEE34 pKa = 3.49GNIIFVYY41 pKa = 10.65

Molecular weight: 5.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

8043

41

780

164.1

18.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.122 ± 0.535

1.318 ± 0.192

5.806 ± 0.325

8.939 ± 0.559

4.538 ± 0.239

5.682 ± 0.487

1.218 ± 0.183

8.479 ± 0.311

9.598 ± 0.487

8.554 ± 0.288

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.86 ± 0.138

6.59 ± 0.335

2.275 ± 0.181

3.245 ± 0.201

4.003 ± 0.292

5.545 ± 0.345

5.545 ± 0.255

5.023 ± 0.269

1.231 ± 0.114

4.426 ± 0.303
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski