Porphyromonas asaccharolytica (strain ATCC 25260 / DSM 20707 / BCRC 10618 / JCM 6326 / LMG 13178 / VPI 4198) (Bacteroides asaccharolyticus)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Porphyromonadaceae; Porphyromonas; Porphyromonas asaccharolytica

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1672 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F4KKC6|F4KKC6_PORAD Uncharacterized protein OS=Porphyromonas asaccharolytica (strain ATCC 25260 / DSM 20707 / BCRC 10618 / JCM 6326 / LMG 13178 / VPI 4198) OX=879243 GN=Poras_0908 PE=4 SV=1
MM1 pKa = 7.72KK2 pKa = 9.62NTGLYY7 pKa = 9.72FGSSTGHH14 pKa = 5.65VEE16 pKa = 4.83GIADD20 pKa = 4.58KK21 pKa = 10.81VAQLLGIDD29 pKa = 3.73AANVHH34 pKa = 5.87NVADD38 pKa = 4.16ATPEE42 pKa = 3.88SALAYY47 pKa = 10.07DD48 pKa = 4.23LLIFGSSTWGYY59 pKa = 11.66GDD61 pKa = 5.61LQDD64 pKa = 4.25DD65 pKa = 4.12WEE67 pKa = 4.79GFLPKK72 pKa = 10.14LVKK75 pKa = 9.92QDD77 pKa = 4.06LSGKK81 pKa = 8.56QFAVFGCGDD90 pKa = 3.45SSSYY94 pKa = 11.05PDD96 pKa = 3.71TFCDD100 pKa = 3.4AMAEE104 pKa = 4.0IAEE107 pKa = 4.33QVTAAGATLVGQIPAEE123 pKa = 4.61GYY125 pKa = 9.35SYY127 pKa = 10.86EE128 pKa = 4.05ATRR131 pKa = 11.84CEE133 pKa = 4.32VDD135 pKa = 3.35GQLIGCCLDD144 pKa = 3.73EE145 pKa = 6.29DD146 pKa = 4.34NEE148 pKa = 4.55SDD150 pKa = 3.73EE151 pKa = 4.41TDD153 pKa = 3.51DD154 pKa = 6.43RR155 pKa = 11.84LDD157 pKa = 3.22TWVQAIQKK165 pKa = 9.76AA166 pKa = 3.58

Molecular weight: 17.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F4KMV5|F4KMV5_PORAD Uncharacterized protein OS=Porphyromonas asaccharolytica (strain ATCC 25260 / DSM 20707 / BCRC 10618 / JCM 6326 / LMG 13178 / VPI 4198) OX=879243 GN=Poras_1399 PE=4 SV=1
MM1 pKa = 6.94GQKK4 pKa = 9.78INPIANRR11 pKa = 11.84LGYY14 pKa = 9.7IRR16 pKa = 11.84GWDD19 pKa = 3.72SNWYY23 pKa = 9.83GGRR26 pKa = 11.84NYY28 pKa = 11.08GDD30 pKa = 3.52TLVEE34 pKa = 4.17DD35 pKa = 3.87DD36 pKa = 5.19RR37 pKa = 11.84IRR39 pKa = 11.84KK40 pKa = 8.65YY41 pKa = 11.04LHH43 pKa = 6.04TRR45 pKa = 11.84LSGALVSRR53 pKa = 11.84IIIEE57 pKa = 4.06RR58 pKa = 11.84ALKK61 pKa = 10.65VITITICTARR71 pKa = 11.84PGMIVGKK78 pKa = 10.44GGQNVEE84 pKa = 3.99NLKK87 pKa = 11.07AEE89 pKa = 4.3LKK91 pKa = 10.68KK92 pKa = 9.74VTKK95 pKa = 10.43ADD97 pKa = 3.85NIQINIYY104 pKa = 7.16EE105 pKa = 4.08VRR107 pKa = 11.84KK108 pKa = 9.77PEE110 pKa = 3.61VDD112 pKa = 3.31AVIVANDD119 pKa = 3.45IARR122 pKa = 11.84KK123 pKa = 9.91LEE125 pKa = 3.41GRR127 pKa = 11.84MSYY130 pKa = 10.47RR131 pKa = 11.84RR132 pKa = 11.84TVKK135 pKa = 9.89MAIANAMRR143 pKa = 11.84AGAEE147 pKa = 4.36GIKK150 pKa = 9.94IQLSGRR156 pKa = 11.84LNNAEE161 pKa = 4.13MARR164 pKa = 11.84SEE166 pKa = 4.25LYY168 pKa = 10.6KK169 pKa = 10.53EE170 pKa = 4.32GRR172 pKa = 11.84TPLHH176 pKa = 5.9TLRR179 pKa = 11.84ADD181 pKa = 2.9IDD183 pKa = 4.02YY184 pKa = 10.29AQTGALTKK192 pKa = 10.73VGMIGVKK199 pKa = 8.65VWIMNGEE206 pKa = 4.09VYY208 pKa = 9.88EE209 pKa = 4.43RR210 pKa = 11.84RR211 pKa = 11.84DD212 pKa = 3.53LAPDD216 pKa = 3.84LSQPRR221 pKa = 11.84RR222 pKa = 11.84QGGRR226 pKa = 11.84RR227 pKa = 11.84GGDD230 pKa = 2.96SRR232 pKa = 11.84GEE234 pKa = 3.56RR235 pKa = 11.84RR236 pKa = 11.84GFRR239 pKa = 11.84RR240 pKa = 11.84GGRR243 pKa = 11.84NRR245 pKa = 11.84SNRR248 pKa = 3.39

Molecular weight: 27.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1672

0

1672

625607

32

2517

374.2

41.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.093 ± 0.052

1.131 ± 0.025

5.439 ± 0.031

6.523 ± 0.055

3.429 ± 0.033

6.805 ± 0.048

2.213 ± 0.028

6.274 ± 0.043

4.698 ± 0.047

10.618 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.451 ± 0.027

3.523 ± 0.046

4.436 ± 0.033

4.38 ± 0.037

5.515 ± 0.056

6.421 ± 0.054

6.274 ± 0.056

6.498 ± 0.043

1.09 ± 0.02

4.187 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski