Pseudallescheria apiosperma (Scedosporium apiospermum)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomyc

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8375 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A084G3H2|A0A084G3H2_PSEDA Fumarylacetoacetase OS=Pseudallescheria apiosperma OX=563466 GN=SAPIO_CDS6573 PE=3 SV=1
MM1 pKa = 7.71KK2 pKa = 10.48SQFLSLYY9 pKa = 9.83GLAALASLGVGAAARR24 pKa = 11.84TAEE27 pKa = 4.13PTVVLSARR35 pKa = 11.84SFKK38 pKa = 11.03HH39 pKa = 4.38EE40 pKa = 4.2ACWTDD45 pKa = 3.24EE46 pKa = 4.41DD47 pKa = 4.63ALSGPGFSSPSMTVRR62 pKa = 11.84WCARR66 pKa = 11.84FCRR69 pKa = 11.84GYY71 pKa = 11.02EE72 pKa = 3.92YY73 pKa = 10.77FGLANGDD80 pKa = 3.41GRR82 pKa = 11.84GSTRR86 pKa = 11.84LSLYY90 pKa = 9.47KK91 pKa = 10.45AKK93 pKa = 10.72EE94 pKa = 3.92GGTCHH99 pKa = 5.95VRR101 pKa = 11.84PSSTRR106 pKa = 11.84AHH108 pKa = 6.15SSEE111 pKa = 4.29SEE113 pKa = 4.06IMTEE117 pKa = 4.07PATSTSASEE126 pKa = 4.1LTSATIEE133 pKa = 4.25SAQTTEE139 pKa = 4.77SITEE143 pKa = 4.09SASATEE149 pKa = 4.53SEE151 pKa = 4.94SPTSTEE157 pKa = 3.86EE158 pKa = 3.97ASEE161 pKa = 4.33STSEE165 pKa = 4.26SAIEE169 pKa = 4.2SDD171 pKa = 3.48TAPVTEE177 pKa = 5.07SEE179 pKa = 4.47TSSATLTQSQPEE191 pKa = 4.3TTFDD195 pKa = 3.5VTTSTSEE202 pKa = 4.12SATEE206 pKa = 3.57TDD208 pKa = 3.17AASTSEE214 pKa = 4.15STVEE218 pKa = 4.1SSSEE222 pKa = 4.06SATEE226 pKa = 4.04LSTEE230 pKa = 4.28SEE232 pKa = 4.4PEE234 pKa = 3.82PTTTAEE240 pKa = 4.33LEE242 pKa = 4.37STTTEE247 pKa = 3.98DD248 pKa = 3.35AAPATTSDD256 pKa = 4.15SEE258 pKa = 4.5SSTTEE263 pKa = 3.79EE264 pKa = 4.54PEE266 pKa = 4.17TTVTSDD272 pKa = 4.45AEE274 pKa = 4.35TTTTKK279 pKa = 10.56EE280 pKa = 4.15PEE282 pKa = 4.44TTTVTPDD289 pKa = 3.02VEE291 pKa = 4.54TTTAEE296 pKa = 4.14EE297 pKa = 4.22PVATTTEE304 pKa = 4.2EE305 pKa = 4.52PEE307 pKa = 4.16TTSAAEE313 pKa = 4.0TTTTEE318 pKa = 3.97EE319 pKa = 4.45SEE321 pKa = 4.36TTSASDD327 pKa = 3.06VDD329 pKa = 3.95TTTTEE334 pKa = 4.1EE335 pKa = 4.18ASTTTTEE342 pKa = 4.15DD343 pKa = 3.9PEE345 pKa = 4.61TKK347 pKa = 9.98TIEE350 pKa = 4.25DD351 pKa = 3.91TSTSTTEE358 pKa = 4.35TPTSTPTPCIDD369 pKa = 4.05CQNLPTGNWLLASTSTGSGILEE391 pKa = 4.15LRR393 pKa = 11.84YY394 pKa = 10.47DD395 pKa = 3.72SAGGYY400 pKa = 8.67YY401 pKa = 9.7YY402 pKa = 10.99VYY404 pKa = 10.37NEE406 pKa = 4.84CPASHH411 pKa = 6.78GGYY414 pKa = 7.54CTVYY418 pKa = 8.03IAQVISVEE426 pKa = 4.07AGITYY431 pKa = 10.38DD432 pKa = 4.42FAIQYY437 pKa = 10.42LMSNVRR443 pKa = 11.84NQANFLQISISTLPSRR459 pKa = 11.84TRR461 pKa = 11.84IFTEE465 pKa = 4.26YY466 pKa = 10.28FSSGSTNGWTDD477 pKa = 3.62FRR479 pKa = 11.84TSPFTPTVSGDD490 pKa = 3.41VLITVNWLNDD500 pKa = 3.72PNNALVLIRR509 pKa = 11.84NIVMQPTEE517 pKa = 4.07CRR519 pKa = 11.84NPLSEE524 pKa = 4.4LTCNTDD530 pKa = 2.96VEE532 pKa = 4.72VQVVV536 pKa = 3.14

Molecular weight: 56.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A084GDE5|A0A084GDE5_PSEDA Ribosome assembly protein 1 OS=Pseudallescheria apiosperma OX=563466 GN=SAPIO_CDS2175 PE=4 SV=1
MM1 pKa = 7.36SALATSQDD9 pKa = 3.58TRR11 pKa = 11.84CTPNISNDD19 pKa = 3.56AFCMTTAKK27 pKa = 10.06RR28 pKa = 11.84HH29 pKa = 5.62RR30 pKa = 11.84PSNTIRR36 pKa = 11.84PRR38 pKa = 11.84IAWADD43 pKa = 3.49TTMTTNPLMVIHH55 pKa = 6.81PPPYY59 pKa = 10.0LNNSAEE65 pKa = 4.15ICRR68 pKa = 11.84SSRR71 pKa = 11.84NDD73 pKa = 2.92IAGIRR78 pKa = 11.84PPRR81 pKa = 11.84RR82 pKa = 11.84GSMVMALVFTPRR94 pKa = 11.84DD95 pKa = 3.29KK96 pKa = 10.96EE97 pKa = 4.07RR98 pKa = 11.84RR99 pKa = 11.84DD100 pKa = 3.43EE101 pKa = 4.12KK102 pKa = 11.41

Molecular weight: 11.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8375

0

8375

4573252

66

8042

546.1

60.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.814 ± 0.027

1.211 ± 0.009

5.854 ± 0.02

6.395 ± 0.034

3.739 ± 0.018

7.095 ± 0.022

2.276 ± 0.011

4.951 ± 0.016

4.78 ± 0.022

8.964 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.082 ± 0.009

3.533 ± 0.015

6.19 ± 0.033

3.763 ± 0.02

6.166 ± 0.02

7.866 ± 0.027

5.842 ± 0.016

6.292 ± 0.019

1.47 ± 0.01

2.718 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski