Beihai sea slater virus 4

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KJK8|A0A1L3KJK8_9VIRU RdRp OS=Beihai sea slater virus 4 OX=1922660 PE=4 SV=1
MM1 pKa = 6.89NRR3 pKa = 11.84NYY5 pKa = 10.0FRR7 pKa = 11.84LVHH10 pKa = 5.85HH11 pKa = 6.36QFNEE15 pKa = 3.86LRR17 pKa = 11.84RR18 pKa = 11.84DD19 pKa = 3.6TQVAYY24 pKa = 10.45ASLAALLLVAGLATTGIFTLQEE46 pKa = 3.39ISEE49 pKa = 4.66KK50 pKa = 10.12IEE52 pKa = 4.12HH53 pKa = 6.85DD54 pKa = 3.89PEE56 pKa = 3.87HH57 pKa = 6.53TIIDD61 pKa = 3.99GVIDD65 pKa = 4.73DD66 pKa = 4.74VIEE69 pKa = 4.19MYY71 pKa = 10.97LNQLFTQPGLASRR84 pKa = 11.84ITALGDD90 pKa = 3.78LIKK93 pKa = 10.53SIKK96 pKa = 9.89EE97 pKa = 3.58IIGPILKK104 pKa = 10.31YY105 pKa = 8.9FTKK108 pKa = 10.74DD109 pKa = 2.62SDD111 pKa = 4.05YY112 pKa = 11.75LLGNVTAILTNITYY126 pKa = 10.86SNLVSDD132 pKa = 6.37FILQEE137 pKa = 4.81LIDD140 pKa = 4.75LNCAIHH146 pKa = 6.3KK147 pKa = 10.22CEE149 pKa = 5.77SIDD152 pKa = 3.72EE153 pKa = 4.78DD154 pKa = 5.07DD155 pKa = 4.33VSDD158 pKa = 3.99MLNSILDD165 pKa = 4.23FIEE168 pKa = 4.3DD169 pKa = 3.77HH170 pKa = 6.76PALPEE175 pKa = 3.73KK176 pKa = 10.8VYY178 pKa = 10.77DD179 pKa = 3.82YY180 pKa = 11.12FKK182 pKa = 10.11TYY184 pKa = 10.22PITAEE189 pKa = 3.87CLHH192 pKa = 6.78FKK194 pKa = 10.63RR195 pKa = 11.84IALLSDD201 pKa = 4.31ADD203 pKa = 4.11TQQQLSARR211 pKa = 11.84PDD213 pKa = 3.17HH214 pKa = 6.28EE215 pKa = 5.15AYY217 pKa = 10.0EE218 pKa = 4.57VVRR221 pKa = 11.84HH222 pKa = 5.49CVSNGVNCQPVRR234 pKa = 11.84YY235 pKa = 9.02EE236 pKa = 4.47SKK238 pKa = 10.97CSDD241 pKa = 2.67GKK243 pKa = 10.77YY244 pKa = 9.91YY245 pKa = 11.04CFDD248 pKa = 4.48DD249 pKa = 4.59GFFAIPTYY257 pKa = 10.71EE258 pKa = 3.93PFSFKK263 pKa = 10.67VLQNEE268 pKa = 4.11DD269 pKa = 3.32WVMEE273 pKa = 4.31GADD276 pKa = 3.94YY277 pKa = 11.08TDD279 pKa = 3.69LVYY282 pKa = 10.45FQGKK286 pKa = 8.38IRR288 pKa = 11.84YY289 pKa = 6.34TPQAYY294 pKa = 8.11TYY296 pKa = 9.85KK297 pKa = 10.28FQYY300 pKa = 9.6LKK302 pKa = 11.02CVDD305 pKa = 4.27GEE307 pKa = 4.17LHH309 pKa = 7.32IYY311 pKa = 8.38TCEE314 pKa = 3.97DD315 pKa = 3.33TVEE318 pKa = 4.1SCEE321 pKa = 4.37LYY323 pKa = 10.9NLEE326 pKa = 4.23TTCYY330 pKa = 10.96GEE332 pKa = 4.04GKK334 pKa = 10.5YY335 pKa = 10.51LFLPMHH341 pKa = 6.59VKK343 pKa = 10.51RR344 pKa = 11.84LDD346 pKa = 3.71EE347 pKa = 4.33TWCSIMPDD355 pKa = 3.34QAEE358 pKa = 4.23SCSIGDD364 pKa = 3.4SWVDD368 pKa = 2.99SFYY371 pKa = 11.09YY372 pKa = 10.33DD373 pKa = 2.94FWYY376 pKa = 10.1FIVNGAFGTHH386 pKa = 4.62VTAEE390 pKa = 4.34FKK392 pKa = 10.82DD393 pKa = 3.64QEE395 pKa = 4.58SFTKK399 pKa = 10.53KK400 pKa = 8.31STAYY404 pKa = 8.79TYY406 pKa = 10.08CHH408 pKa = 6.72KK409 pKa = 10.27CDD411 pKa = 3.57EE412 pKa = 4.66GPYY415 pKa = 9.71PSGSEE420 pKa = 4.26LLIAMDD426 pKa = 3.81NFVNASFPTQEE437 pKa = 3.75STYY440 pKa = 11.24GGMGNGKK447 pKa = 8.56HH448 pKa = 6.19LCLNKK453 pKa = 10.49YY454 pKa = 10.38NLFKK458 pKa = 11.12

Molecular weight: 52.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KJP7|A0A1L3KJP7_9VIRU Uncharacterized protein OS=Beihai sea slater virus 4 OX=1922660 PE=4 SV=1
MM1 pKa = 7.93IMAKK5 pKa = 10.42GDD7 pKa = 3.8DD8 pKa = 4.19LYY10 pKa = 11.05IHH12 pKa = 7.18AKK14 pKa = 10.28KK15 pKa = 10.32IIQKK19 pKa = 10.21HH20 pKa = 4.09FDD22 pKa = 3.39KK23 pKa = 10.76NFKK26 pKa = 9.52ISRR29 pKa = 11.84PIVPSFVGFIIYY41 pKa = 10.07DD42 pKa = 3.74KK43 pKa = 10.99LYY45 pKa = 11.11LDD47 pKa = 3.77IPKK50 pKa = 9.17LTVRR54 pKa = 11.84VLNRR58 pKa = 11.84NYY60 pKa = 10.97ADD62 pKa = 3.5NKK64 pKa = 10.49SISDD68 pKa = 3.72YY69 pKa = 10.91IIAVKK74 pKa = 10.42DD75 pKa = 3.23WLSVIKK81 pKa = 10.32SQEE84 pKa = 3.89HH85 pKa = 5.08LTVCSILNAKK95 pKa = 10.21RR96 pKa = 11.84YY97 pKa = 8.07GLSVAEE103 pKa = 4.51CEE105 pKa = 4.28YY106 pKa = 11.57LLGFLFAFSRR116 pKa = 11.84GRR118 pKa = 11.84IINTTNSKK126 pKa = 10.06ILVRR130 pKa = 11.84EE131 pKa = 4.16RR132 pKa = 11.84KK133 pKa = 9.27QPFVLNEE140 pKa = 3.7

Molecular weight: 16.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

4210

140

2462

526.3

59.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.081 ± 0.545

2.09 ± 0.266

6.722 ± 0.635

6.01 ± 0.764

4.679 ± 0.431

4.537 ± 0.358

2.779 ± 0.272

6.318 ± 0.659

7.007 ± 1.385

8.622 ± 0.837

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.09 ± 0.27

4.751 ± 0.291

4.276 ± 0.332

3.492 ± 0.256

4.751 ± 0.497

6.675 ± 0.788

6.698 ± 0.476

7.031 ± 0.402

0.998 ± 0.163

4.394 ± 0.649
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski