Helicobacter phage UKEN32U

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Schmidvirus; unclassified Schmidvirus

Average proteome isoelectric point is 7.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 35 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5RH61|A0A1S5RH61_9CAUD Mitogen-activated protein kinase 1 OS=Helicobacter phage UKEN32U OX=1852688 PE=4 SV=1
MM1 pKa = 7.66GIKK4 pKa = 9.82EE5 pKa = 4.23KK6 pKa = 10.55EE7 pKa = 3.99IEE9 pKa = 4.18LEE11 pKa = 3.99TLKK14 pKa = 11.01RR15 pKa = 11.84EE16 pKa = 3.99IAQAEE21 pKa = 4.06ASLEE25 pKa = 3.89QDD27 pKa = 4.59FIKK30 pKa = 10.93HH31 pKa = 5.17MVDD34 pKa = 2.85KK35 pKa = 10.51TSEE38 pKa = 4.11KK39 pKa = 10.83VEE41 pKa = 4.46DD42 pKa = 3.98LFFSNKK48 pKa = 8.85PEE50 pKa = 4.29FYY52 pKa = 10.01RR53 pKa = 11.84FVFNEE58 pKa = 3.46QNKK61 pKa = 8.73YY62 pKa = 10.52LRR64 pKa = 11.84EE65 pKa = 3.98KK66 pKa = 10.29LTDD69 pKa = 3.41KK70 pKa = 10.83VGRR73 pKa = 11.84AMDD76 pKa = 4.55LSDD79 pKa = 5.66EE80 pKa = 4.18IQNDD84 pKa = 2.98KK85 pKa = 10.97DD86 pKa = 3.56AEE88 pKa = 4.21EE89 pKa = 4.15IEE91 pKa = 4.13KK92 pKa = 11.03DD93 pKa = 3.3KK94 pKa = 11.44EE95 pKa = 4.14AFLKK99 pKa = 10.14KK100 pKa = 10.06HH101 pKa = 6.08PEE103 pKa = 3.38IDD105 pKa = 3.74FNEE108 pKa = 3.95LLEE111 pKa = 4.62FYY113 pKa = 10.85NEE115 pKa = 4.02EE116 pKa = 3.77VPNRR120 pKa = 11.84IKK122 pKa = 10.86KK123 pKa = 10.42QIDD126 pKa = 3.39KK127 pKa = 11.12LEE129 pKa = 4.21GVAFFEE135 pKa = 5.24AVLDD139 pKa = 3.87YY140 pKa = 11.19FNALNAKK147 pKa = 9.33EE148 pKa = 4.37EE149 pKa = 4.21EE150 pKa = 4.46PKK152 pKa = 10.95NEE154 pKa = 4.05AKK156 pKa = 10.59EE157 pKa = 4.24EE158 pKa = 3.97EE159 pKa = 4.33SQLPKK164 pKa = 10.37EE165 pKa = 4.03ALGNGVSGVGNASNEE180 pKa = 4.31NIMTRR185 pKa = 11.84YY186 pKa = 9.53

Molecular weight: 21.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5RH52|A0A1S5RH52_9CAUD Putative terminase OS=Helicobacter phage UKEN32U OX=1852688 PE=4 SV=1
MM1 pKa = 7.44LNAIKK6 pKa = 10.31FRR8 pKa = 11.84IYY10 pKa = 10.58PNAQQKK16 pKa = 9.41EE17 pKa = 4.62LISKK21 pKa = 10.01HH22 pKa = 5.57FGCSRR27 pKa = 11.84VVYY30 pKa = 10.47NYY32 pKa = 10.55FLDD35 pKa = 3.97YY36 pKa = 10.43RR37 pKa = 11.84QKK39 pKa = 10.83QYY41 pKa = 11.81AKK43 pKa = 10.14GIKK46 pKa = 8.01EE47 pKa = 4.53TYY49 pKa = 8.68FTMQKK54 pKa = 10.2VLTQIKK60 pKa = 9.44RR61 pKa = 11.84QEE63 pKa = 3.97KK64 pKa = 8.17YY65 pKa = 10.47HH66 pKa = 6.16YY67 pKa = 10.84LNEE70 pKa = 4.56CNSQSLQMALRR81 pKa = 11.84QLVSAYY87 pKa = 10.93DD88 pKa = 3.42NFFSKK93 pKa = 10.5RR94 pKa = 11.84ARR96 pKa = 11.84YY97 pKa = 8.55PKK99 pKa = 10.37FKK101 pKa = 10.41SKK103 pKa = 10.76KK104 pKa = 7.81NAKK107 pKa = 9.36QSFAIPQNIEE117 pKa = 3.39IKK119 pKa = 11.0AEE121 pKa = 4.12TKK123 pKa = 10.19TIALPKK129 pKa = 9.84FKK131 pKa = 10.72EE132 pKa = 4.62GIKK135 pKa = 10.62AKK137 pKa = 9.61LHH139 pKa = 6.41RR140 pKa = 11.84DD141 pKa = 3.43LPKK144 pKa = 11.01DD145 pKa = 3.71SVIKK149 pKa = 10.32QAFISCIADD158 pKa = 3.12QYY160 pKa = 10.88FCSLSYY166 pKa = 8.8EE167 pKa = 4.33TKK169 pKa = 10.58EE170 pKa = 4.6PIPKK174 pKa = 8.77PTIIKK179 pKa = 10.01KK180 pKa = 10.46AVGLDD185 pKa = 3.03MGLRR189 pKa = 11.84TLIVTSDD196 pKa = 3.47KK197 pKa = 10.91IEE199 pKa = 4.06YY200 pKa = 8.68PHH202 pKa = 7.5IRR204 pKa = 11.84FYY206 pKa = 11.37QKK208 pKa = 10.84LEE210 pKa = 4.0KK211 pKa = 10.51KK212 pKa = 8.72LTKK215 pKa = 9.84AQRR218 pKa = 11.84RR219 pKa = 11.84LSKK222 pKa = 10.28KK223 pKa = 10.36VKK225 pKa = 10.08GSNNRR230 pKa = 11.84KK231 pKa = 8.63KK232 pKa = 9.9QAKK235 pKa = 9.04KK236 pKa = 10.2VSRR239 pKa = 11.84LHH241 pKa = 6.25LACSNTRR248 pKa = 11.84DD249 pKa = 4.0DD250 pKa = 4.33YY251 pKa = 11.62LHH253 pKa = 6.99KK254 pKa = 10.44ISNEE258 pKa = 3.23ITNQYY263 pKa = 10.97DD264 pKa = 4.17LIGVEE269 pKa = 4.16TLNIKK274 pKa = 10.64ALMKK278 pKa = 9.63TYY280 pKa = 10.22HH281 pKa = 6.41SKK283 pKa = 10.96SLANASWGKK292 pKa = 9.22FLAMLEE298 pKa = 4.21YY299 pKa = 10.38KK300 pKa = 10.32AQRR303 pKa = 11.84KK304 pKa = 9.03GKK306 pKa = 7.34TLLRR310 pKa = 11.84IDD312 pKa = 3.95RR313 pKa = 11.84FFPSSQLCSYY323 pKa = 10.69CGVNTGKK330 pKa = 9.97KK331 pKa = 9.39HH332 pKa = 5.53EE333 pKa = 5.31SITKK337 pKa = 7.35FTCPHH342 pKa = 6.91CKK344 pKa = 7.53TTHH347 pKa = 5.66HH348 pKa = 7.02RR349 pKa = 11.84DD350 pKa = 3.21YY351 pKa = 10.78NASVNIRR358 pKa = 11.84NYY360 pKa = 10.82ALGMLDD366 pKa = 4.8DD367 pKa = 3.81RR368 pKa = 11.84HH369 pKa = 6.14KK370 pKa = 11.43VKK372 pKa = 10.0TDD374 pKa = 3.04KK375 pKa = 11.22ARR377 pKa = 11.84VGIIRR382 pKa = 11.84SDD384 pKa = 3.75YY385 pKa = 7.91THH387 pKa = 6.78HH388 pKa = 6.81TNEE391 pKa = 4.28CVKK394 pKa = 11.0ACGASSNGVSSKK406 pKa = 10.83YY407 pKa = 11.2GNILDD412 pKa = 4.17LASYY416 pKa = 10.91GAMKK420 pKa = 10.18QEE422 pKa = 4.59KK423 pKa = 9.54AQSLL427 pKa = 3.89

Molecular weight: 49.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

35

0

35

9506

48

1724

271.6

31.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.627 ± 0.347

0.799 ± 0.213

4.881 ± 0.49

8.405 ± 0.405

4.892 ± 0.295

4.176 ± 0.254

1.42 ± 0.186

7.122 ± 0.273

10.814 ± 0.484

10.793 ± 0.321

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.704 ± 0.191

8.121 ± 0.479

2.356 ± 0.188

4.66 ± 0.392

3.766 ± 0.251

6.47 ± 0.271

4.86 ± 0.397

4.166 ± 0.36

0.442 ± 0.077

3.524 ± 0.256
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski