Treponema brennaborense (strain DSM 12168 / CIP 105900 / DD5/3)

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Spirochaetales; Treponemataceae; Treponema; Treponema brennaborense

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2522 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F4LML1|F4LML1_TREBD Uncharacterized protein OS=Treponema brennaborense (strain DSM 12168 / CIP 105900 / DD5/3) OX=906968 GN=Trebr_1333 PE=4 SV=1
MM1 pKa = 7.19YY2 pKa = 10.82VNNDD6 pKa = 3.22PVNYY10 pKa = 9.59VDD12 pKa = 5.39LWGLSAGDD20 pKa = 4.59AKK22 pKa = 10.87SVWDD26 pKa = 4.08GFTGAGNPYY35 pKa = 10.54ANYY38 pKa = 10.06GVSTIIDD45 pKa = 3.73STLSSDD51 pKa = 3.55IAEE54 pKa = 4.22KK55 pKa = 7.46TTNYY59 pKa = 10.19VNDD62 pKa = 4.46FRR64 pKa = 11.84CDD66 pKa = 3.09MYY68 pKa = 11.29AWNSAVSDD76 pKa = 3.76GVNPKK81 pKa = 9.52GQNGEE86 pKa = 3.88DD87 pKa = 3.27WDD89 pKa = 3.73IDD91 pKa = 3.79RR92 pKa = 11.84EE93 pKa = 4.58SVSDD97 pKa = 3.86IYY99 pKa = 11.78NKK101 pKa = 10.16FPVLRR106 pKa = 11.84FVAPQNNTKK115 pKa = 10.37GFAFYY120 pKa = 10.55FNDD123 pKa = 3.57MNDD126 pKa = 3.53EE127 pKa = 4.25PVHH130 pKa = 7.51IEE132 pKa = 4.22YY133 pKa = 10.93YY134 pKa = 11.0DD135 pKa = 3.49NTNDD139 pKa = 3.19VSEE142 pKa = 4.85TYY144 pKa = 10.39VQYY147 pKa = 9.4KK148 pKa = 9.55TDD150 pKa = 3.25GKK152 pKa = 10.48NAVEE156 pKa = 4.17TVDD159 pKa = 5.15RR160 pKa = 11.84KK161 pKa = 10.77INDD164 pKa = 3.58DD165 pKa = 3.74RR166 pKa = 11.84NLSVVYY172 pKa = 9.12VVIYY176 pKa = 10.9SEE178 pKa = 4.14

Molecular weight: 20.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F4LNE6|F4LNE6_TREBD Putative signal transduction protein OS=Treponema brennaborense (strain DSM 12168 / CIP 105900 / DD5/3) OX=906968 GN=Trebr_2498 PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 10.03ISAPHH7 pKa = 6.1PVRR10 pKa = 11.84TGTSLTVRR18 pKa = 11.84FTATALFLLAAAAAGIAFGTEE39 pKa = 4.06RR40 pKa = 11.84IPFRR44 pKa = 11.84EE45 pKa = 3.73IGNALFRR52 pKa = 11.84PAAADD57 pKa = 3.3PFAAVIVRR65 pKa = 11.84EE66 pKa = 3.94LRR68 pKa = 11.84LPRR71 pKa = 11.84VLLAGLSGALLASAGAAFQGFFRR94 pKa = 11.84NPLAEE99 pKa = 4.21SGIMGISSGAALGAVLSAFIPAATGIAGAAEE130 pKa = 4.03LASRR134 pKa = 11.84FPQFMQANVTTVCAFCGAAAAAFLIYY160 pKa = 10.25AASKK164 pKa = 8.7LTARR168 pKa = 11.84SGGTIAILLTGTAAGTFFSAVTSVVLLVKK197 pKa = 9.89DD198 pKa = 4.05TEE200 pKa = 4.21LHH202 pKa = 7.06RR203 pKa = 11.84MFVWTLGSFNGKK215 pKa = 8.56GWSEE219 pKa = 3.94LAFVLPAAVLSAVLLAGCARR239 pKa = 11.84FLDD242 pKa = 3.96VLSGGEE248 pKa = 3.9RR249 pKa = 11.84TAQALGLDD257 pKa = 4.05PAHH260 pKa = 6.93ARR262 pKa = 11.84LLVLTAGSLASAAAVCAGGTIGFVGLIAPHH292 pKa = 4.99VARR295 pKa = 11.84RR296 pKa = 11.84LFSPRR301 pKa = 11.84HH302 pKa = 5.15AVLIPASMMGGAVFLITCDD321 pKa = 3.1TAARR325 pKa = 11.84TVAAPAEE332 pKa = 4.23IPVGIITALAGAPFFISILFGGRR355 pKa = 11.84NRR357 pKa = 11.84TGNFNEE363 pKa = 4.18

Molecular weight: 36.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2522

0

2522

908311

37

3722

360.2

39.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.076 ± 0.084

1.456 ± 0.018

5.441 ± 0.037

6.136 ± 0.058

4.719 ± 0.04

7.16 ± 0.048

1.48 ± 0.019

6.39 ± 0.046

5.406 ± 0.045

9.214 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.255 ± 0.025

3.807 ± 0.039

4.08 ± 0.036

2.955 ± 0.024

5.17 ± 0.046

6.677 ± 0.042

5.884 ± 0.046

7.152 ± 0.045

0.948 ± 0.014

3.593 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski