Brevundimonas sp. SH203

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales; Caulobacteraceae; Brevundimonas; unclassified Brevundimonas

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2958 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V1V094|A0A1V1V094_9CAUL Uncharacterized protein OS=Brevundimonas sp. SH203 OX=345167 GN=SH203_01961 PE=4 SV=1
MM1 pKa = 7.19TADD4 pKa = 3.38DD5 pKa = 4.71TIYY8 pKa = 10.43QAEE11 pKa = 5.08GYY13 pKa = 9.32MDD15 pKa = 6.31DD16 pKa = 5.17DD17 pKa = 3.79LHH19 pKa = 6.63EE20 pKa = 5.7ADD22 pKa = 3.01IVEE25 pKa = 4.49WYY27 pKa = 7.65EE28 pKa = 3.72ARR30 pKa = 11.84PVTVSTAVAAGAVVTAFTLGALAAVGALFLIGRR63 pKa = 11.84LDD65 pKa = 3.59DD66 pKa = 3.75

Molecular weight: 6.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V1V124|A0A1V1V124_9CAUL Competence protein ComM OS=Brevundimonas sp. SH203 OX=345167 GN=comM PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.2QPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 8.99RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.7GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.2NGQKK29 pKa = 9.53IVARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.77GRR39 pKa = 11.84KK40 pKa = 9.04RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2958

0

2958

942333

30

1827

318.6

34.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.879 ± 0.074

0.682 ± 0.012

6.169 ± 0.035

5.313 ± 0.046

3.477 ± 0.028

8.981 ± 0.04

1.756 ± 0.021

4.391 ± 0.027

2.858 ± 0.036

9.813 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.306 ± 0.022

2.429 ± 0.034

5.417 ± 0.035

3.28 ± 0.025

7.544 ± 0.049

5.004 ± 0.032

5.397 ± 0.037

7.708 ± 0.039

1.475 ± 0.02

2.12 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski