Amino acid dipepetide frequency for Arthrobacter phage BarretLemon

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.906AlaAla: 17.906 ± 1.753
0.558AlaCys: 0.558 ± 0.175
6.815AlaAsp: 6.815 ± 1.099
8.426AlaGlu: 8.426 ± 0.917
2.974AlaPhe: 2.974 ± 0.497
9.17AlaGly: 9.17 ± 0.916
2.478AlaHis: 2.478 ± 0.45
4.585AlaIle: 4.585 ± 0.512
4.771AlaLys: 4.771 ± 0.623
10.409AlaLeu: 10.409 ± 0.967
2.912AlaMet: 2.912 ± 0.359
3.346AlaAsn: 3.346 ± 0.465
5.328AlaPro: 5.328 ± 0.807
4.833AlaGln: 4.833 ± 0.547
7.621AlaArg: 7.621 ± 0.883
5.019AlaSer: 5.019 ± 0.598
7.125AlaThr: 7.125 ± 0.674
8.055AlaVal: 8.055 ± 0.862
1.115AlaTrp: 1.115 ± 0.268
2.107AlaTyr: 2.107 ± 0.296
0.0AlaXaa: 0.0 ± 0.0
Cys
0.434CysAla: 0.434 ± 0.141
0.062CysCys: 0.062 ± 0.059
0.248CysAsp: 0.248 ± 0.113
0.434CysGlu: 0.434 ± 0.15
0.186CysPhe: 0.186 ± 0.092
0.434CysGly: 0.434 ± 0.175
0.124CysHis: 0.124 ± 0.089
0.062CysIle: 0.062 ± 0.059
0.434CysLys: 0.434 ± 0.22
0.805CysLeu: 0.805 ± 0.232
0.062CysMet: 0.062 ± 0.068
0.124CysAsn: 0.124 ± 0.082
0.434CysPro: 0.434 ± 0.184
0.186CysGln: 0.186 ± 0.104
0.434CysArg: 0.434 ± 0.231
0.434CysSer: 0.434 ± 0.168
0.434CysThr: 0.434 ± 0.169
0.805CysVal: 0.805 ± 0.188
0.186CysTrp: 0.186 ± 0.106
0.186CysTyr: 0.186 ± 0.1
0.0CysXaa: 0.0 ± 0.0
Asp
8.426AspAla: 8.426 ± 0.779
0.682AspCys: 0.682 ± 0.247
4.399AspAsp: 4.399 ± 0.688
5.7AspGlu: 5.7 ± 0.735
2.045AspPhe: 2.045 ± 0.483
5.762AspGly: 5.762 ± 0.569
0.929AspHis: 0.929 ± 0.249
2.788AspIle: 2.788 ± 0.355
3.284AspLys: 3.284 ± 0.407
3.222AspLeu: 3.222 ± 0.474
1.053AspMet: 1.053 ± 0.215
1.983AspAsn: 1.983 ± 0.379
2.788AspPro: 2.788 ± 0.445
1.859AspGln: 1.859 ± 0.425
2.974AspArg: 2.974 ± 0.394
3.47AspSer: 3.47 ± 0.514
2.726AspThr: 2.726 ± 0.422
6.196AspVal: 6.196 ± 0.535
1.301AspTrp: 1.301 ± 0.257
1.301AspTyr: 1.301 ± 0.256
0.0AspXaa: 0.0 ± 0.0
Glu
6.258GluAla: 6.258 ± 0.744
0.372GluCys: 0.372 ± 0.153
3.779GluAsp: 3.779 ± 0.548
3.656GluGlu: 3.656 ± 0.457
1.673GluPhe: 1.673 ± 0.337
3.036GluGly: 3.036 ± 0.541
1.549GluHis: 1.549 ± 0.333
3.408GluIle: 3.408 ± 0.446
2.912GluLys: 2.912 ± 0.468
7.869GluLeu: 7.869 ± 0.868
1.673GluMet: 1.673 ± 0.341
1.983GluAsn: 1.983 ± 0.323
4.151GluPro: 4.151 ± 0.485
3.532GluGln: 3.532 ± 0.446
4.709GluArg: 4.709 ± 0.614
4.027GluSer: 4.027 ± 0.51
3.408GluThr: 3.408 ± 0.457
3.408GluVal: 3.408 ± 0.495
0.929GluTrp: 0.929 ± 0.208
1.611GluTyr: 1.611 ± 0.303
0.0GluXaa: 0.0 ± 0.0
Phe
3.098PheAla: 3.098 ± 0.473
0.248PheCys: 0.248 ± 0.111
2.478PheAsp: 2.478 ± 0.334
2.54PheGlu: 2.54 ± 0.406
0.434PhePhe: 0.434 ± 0.201
2.54PheGly: 2.54 ± 0.426
0.31PheHis: 0.31 ± 0.147
1.363PheIle: 1.363 ± 0.232
1.921PheLys: 1.921 ± 0.33
1.487PheLeu: 1.487 ± 0.292
1.115PheMet: 1.115 ± 0.209
1.115PheAsn: 1.115 ± 0.25
1.487PhePro: 1.487 ± 0.297
0.62PheGln: 0.62 ± 0.178
1.239PheArg: 1.239 ± 0.238
2.107PheSer: 2.107 ± 0.361
2.292PheThr: 2.292 ± 0.384
2.354PheVal: 2.354 ± 0.313
0.496PheTrp: 0.496 ± 0.18
0.496PheTyr: 0.496 ± 0.146
0.0PheXaa: 0.0 ± 0.0
Gly
7.187GlyAla: 7.187 ± 0.6
0.186GlyCys: 0.186 ± 0.103
4.957GlyAsp: 4.957 ± 0.541
4.337GlyGlu: 4.337 ± 0.611
2.045GlyPhe: 2.045 ± 0.44
6.815GlyGly: 6.815 ± 1.296
1.425GlyHis: 1.425 ± 0.26
3.532GlyIle: 3.532 ± 0.489
4.213GlyLys: 4.213 ± 0.612
5.019GlyLeu: 5.019 ± 0.6
1.549GlyMet: 1.549 ± 0.234
3.16GlyAsn: 3.16 ± 0.537
2.354GlyPro: 2.354 ± 0.535
1.921GlyGln: 1.921 ± 0.407
4.275GlyArg: 4.275 ± 0.518
4.895GlySer: 4.895 ± 0.676
5.143GlyThr: 5.143 ± 0.732
6.01GlyVal: 6.01 ± 0.645
2.107GlyTrp: 2.107 ± 0.415
2.416GlyTyr: 2.416 ± 0.329
0.0GlyXaa: 0.0 ± 0.0
His
2.169HisAla: 2.169 ± 0.393
0.124HisCys: 0.124 ± 0.094
0.991HisAsp: 0.991 ± 0.26
1.177HisGlu: 1.177 ± 0.253
0.805HisPhe: 0.805 ± 0.202
0.867HisGly: 0.867 ± 0.245
0.372HisHis: 0.372 ± 0.151
0.867HisIle: 0.867 ± 0.223
0.867HisLys: 0.867 ± 0.197
1.301HisLeu: 1.301 ± 0.283
0.496HisMet: 0.496 ± 0.185
0.805HisAsn: 0.805 ± 0.268
0.682HisPro: 0.682 ± 0.253
0.496HisGln: 0.496 ± 0.199
0.991HisArg: 0.991 ± 0.24
0.62HisSer: 0.62 ± 0.207
1.673HisThr: 1.673 ± 0.393
2.045HisVal: 2.045 ± 0.36
0.434HisTrp: 0.434 ± 0.14
0.434HisTyr: 0.434 ± 0.15
0.0HisXaa: 0.0 ± 0.0
Ile
6.134IleAla: 6.134 ± 0.59
0.372IleCys: 0.372 ± 0.145
3.47IleAsp: 3.47 ± 0.473
4.771IleGlu: 4.771 ± 0.615
1.301IlePhe: 1.301 ± 0.232
4.089IleGly: 4.089 ± 0.727
0.248IleHis: 0.248 ± 0.117
2.788IleIle: 2.788 ± 0.492
2.292IleLys: 2.292 ± 0.364
2.416IleLeu: 2.416 ± 0.413
1.363IleMet: 1.363 ± 0.269
1.797IleAsn: 1.797 ± 0.321
1.487IlePro: 1.487 ± 0.354
1.177IleGln: 1.177 ± 0.273
1.921IleArg: 1.921 ± 0.309
3.717IleSer: 3.717 ± 0.525
3.408IleThr: 3.408 ± 0.388
4.399IleVal: 4.399 ± 0.486
0.558IleTrp: 0.558 ± 0.169
1.115IleTyr: 1.115 ± 0.216
0.0IleXaa: 0.0 ± 0.0
Lys
5.452LysAla: 5.452 ± 0.505
0.434LysCys: 0.434 ± 0.187
2.23LysAsp: 2.23 ± 0.399
1.859LysGlu: 1.859 ± 0.331
1.673LysPhe: 1.673 ± 0.296
3.717LysGly: 3.717 ± 0.513
0.929LysHis: 0.929 ± 0.275
2.602LysIle: 2.602 ± 0.469
0.991LysLys: 0.991 ± 0.26
5.452LysLeu: 5.452 ± 0.611
1.363LysMet: 1.363 ± 0.301
1.177LysAsn: 1.177 ± 0.302
2.354LysPro: 2.354 ± 0.436
2.912LysGln: 2.912 ± 0.438
3.717LysArg: 3.717 ± 0.501
2.85LysSer: 2.85 ± 0.474
2.974LysThr: 2.974 ± 0.424
2.664LysVal: 2.664 ± 0.39
0.62LysTrp: 0.62 ± 0.201
1.239LysTyr: 1.239 ± 0.307
0.0LysXaa: 0.0 ± 0.0
Leu
8.055LeuAla: 8.055 ± 0.764
0.186LeuCys: 0.186 ± 0.103
4.895LeuAsp: 4.895 ± 0.448
3.965LeuGlu: 3.965 ± 0.436
1.859LeuPhe: 1.859 ± 0.278
6.072LeuGly: 6.072 ± 0.849
1.239LeuHis: 1.239 ± 0.341
3.903LeuIle: 3.903 ± 0.581
4.337LeuLys: 4.337 ± 0.435
7.001LeuLeu: 7.001 ± 0.929
1.921LeuMet: 1.921 ± 0.427
2.788LeuAsn: 2.788 ± 0.343
3.903LeuPro: 3.903 ± 0.552
3.47LeuGln: 3.47 ± 0.385
6.134LeuArg: 6.134 ± 0.614
5.576LeuSer: 5.576 ± 0.623
6.815LeuThr: 6.815 ± 0.677
6.01LeuVal: 6.01 ± 0.496
0.929LeuTrp: 0.929 ± 0.266
1.735LeuTyr: 1.735 ± 0.268
0.0LeuXaa: 0.0 ± 0.0
Met
2.602MetAla: 2.602 ± 0.451
0.186MetCys: 0.186 ± 0.116
0.743MetAsp: 0.743 ± 0.201
0.558MetGlu: 0.558 ± 0.24
0.743MetPhe: 0.743 ± 0.194
1.115MetGly: 1.115 ± 0.231
0.31MetHis: 0.31 ± 0.118
0.991MetIle: 0.991 ± 0.243
1.363MetLys: 1.363 ± 0.308
1.673MetLeu: 1.673 ± 0.334
0.558MetMet: 0.558 ± 0.224
1.115MetAsn: 1.115 ± 0.293
1.735MetPro: 1.735 ± 0.407
1.115MetGln: 1.115 ± 0.279
1.611MetArg: 1.611 ± 0.317
2.416MetSer: 2.416 ± 0.329
2.912MetThr: 2.912 ± 0.305
0.805MetVal: 0.805 ± 0.211
0.31MetTrp: 0.31 ± 0.106
0.434MetTyr: 0.434 ± 0.152
0.0MetXaa: 0.0 ± 0.0
Asn
3.779AsnAla: 3.779 ± 0.51
0.558AsnCys: 0.558 ± 0.185
2.045AsnAsp: 2.045 ± 0.429
2.478AsnGlu: 2.478 ± 0.375
0.867AsnPhe: 0.867 ± 0.202
2.85AsnGly: 2.85 ± 0.403
0.62AsnHis: 0.62 ± 0.208
1.859AsnIle: 1.859 ± 0.383
1.673AsnLys: 1.673 ± 0.354
2.974AsnLeu: 2.974 ± 0.363
0.682AsnMet: 0.682 ± 0.212
1.301AsnAsn: 1.301 ± 0.395
2.602AsnPro: 2.602 ± 0.408
0.991AsnGln: 0.991 ± 0.28
1.487AsnArg: 1.487 ± 0.295
1.363AsnSer: 1.363 ± 0.263
2.664AsnThr: 2.664 ± 0.461
2.788AsnVal: 2.788 ± 0.424
0.805AsnTrp: 0.805 ± 0.216
0.682AsnTyr: 0.682 ± 0.26
0.0AsnXaa: 0.0 ± 0.0
Pro
7.373ProAla: 7.373 ± 1.037
0.31ProCys: 0.31 ± 0.114
3.594ProAsp: 3.594 ± 0.528
3.16ProGlu: 3.16 ± 0.387
1.177ProPhe: 1.177 ± 0.232
3.532ProGly: 3.532 ± 0.541
1.363ProHis: 1.363 ± 0.317
2.788ProIle: 2.788 ± 0.531
2.23ProLys: 2.23 ± 0.338
3.47ProLeu: 3.47 ± 0.461
0.805ProMet: 0.805 ± 0.196
2.045ProAsn: 2.045 ± 0.429
2.54ProPro: 2.54 ± 0.639
1.735ProGln: 1.735 ± 0.296
2.107ProArg: 2.107 ± 0.362
2.54ProSer: 2.54 ± 0.461
2.974ProThr: 2.974 ± 0.47
2.85ProVal: 2.85 ± 0.48
0.558ProTrp: 0.558 ± 0.194
1.177ProTyr: 1.177 ± 0.293
0.0ProXaa: 0.0 ± 0.0
Gln
4.647GlnAla: 4.647 ± 0.56
0.434GlnCys: 0.434 ± 0.139
1.363GlnAsp: 1.363 ± 0.275
1.549GlnGlu: 1.549 ± 0.33
1.301GlnPhe: 1.301 ± 0.315
2.169GlnGly: 2.169 ± 0.417
1.115GlnHis: 1.115 ± 0.275
2.23GlnIle: 2.23 ± 0.399
1.549GlnLys: 1.549 ± 0.252
3.47GlnLeu: 3.47 ± 0.557
1.177GlnMet: 1.177 ± 0.26
1.239GlnAsn: 1.239 ± 0.27
2.107GlnPro: 2.107 ± 0.324
2.788GlnGln: 2.788 ± 0.563
2.912GlnArg: 2.912 ± 0.445
2.292GlnSer: 2.292 ± 0.433
2.726GlnThr: 2.726 ± 0.411
2.292GlnVal: 2.292 ± 0.351
0.496GlnTrp: 0.496 ± 0.147
0.743GlnTyr: 0.743 ± 0.222
0.0GlnXaa: 0.0 ± 0.0
Arg
6.196ArgAla: 6.196 ± 0.49
0.434ArgCys: 0.434 ± 0.176
3.656ArgAsp: 3.656 ± 0.441
4.895ArgGlu: 4.895 ± 0.729
2.045ArgPhe: 2.045 ± 0.424
4.213ArgGly: 4.213 ± 0.49
0.991ArgHis: 0.991 ± 0.226
2.54ArgIle: 2.54 ± 0.536
3.284ArgLys: 3.284 ± 0.429
4.771ArgLeu: 4.771 ± 0.601
1.115ArgMet: 1.115 ± 0.242
2.23ArgAsn: 2.23 ± 0.412
3.284ArgPro: 3.284 ± 0.538
2.23ArgGln: 2.23 ± 0.382
3.965ArgArg: 3.965 ± 0.751
3.841ArgSer: 3.841 ± 0.5
3.346ArgThr: 3.346 ± 0.555
5.019ArgVal: 5.019 ± 0.591
1.735ArgTrp: 1.735 ± 0.327
1.983ArgTyr: 1.983 ± 0.388
0.0ArgXaa: 0.0 ± 0.0
Ser
6.32SerAla: 6.32 ± 0.747
0.434SerCys: 0.434 ± 0.171
4.151SerAsp: 4.151 ± 0.424
3.717SerGlu: 3.717 ± 0.476
2.354SerPhe: 2.354 ± 0.336
3.903SerGly: 3.903 ± 0.751
0.929SerHis: 0.929 ± 0.271
3.408SerIle: 3.408 ± 0.473
2.788SerLys: 2.788 ± 0.433
4.709SerLeu: 4.709 ± 0.643
1.549SerMet: 1.549 ± 0.257
1.921SerAsn: 1.921 ± 0.335
2.664SerPro: 2.664 ± 0.406
1.797SerGln: 1.797 ± 0.366
3.532SerArg: 3.532 ± 0.4
4.523SerSer: 4.523 ± 0.571
4.647SerThr: 4.647 ± 0.727
5.081SerVal: 5.081 ± 0.52
1.301SerTrp: 1.301 ± 0.253
1.611SerTyr: 1.611 ± 0.299
0.0SerXaa: 0.0 ± 0.0
Thr
8.116ThrAla: 8.116 ± 0.769
0.558ThrCys: 0.558 ± 0.175
4.089ThrAsp: 4.089 ± 0.521
3.036ThrGlu: 3.036 ± 0.421
2.602ThrPhe: 2.602 ± 0.386
4.647ThrGly: 4.647 ± 0.498
1.611ThrHis: 1.611 ± 0.332
3.16ThrIle: 3.16 ± 0.445
2.85ThrLys: 2.85 ± 0.423
6.629ThrLeu: 6.629 ± 0.578
0.991ThrMet: 0.991 ± 0.265
2.354ThrAsn: 2.354 ± 0.386
3.036ThrPro: 3.036 ± 0.491
2.54ThrGln: 2.54 ± 0.431
4.027ThrArg: 4.027 ± 0.439
4.895ThrSer: 4.895 ± 0.767
5.7ThrThr: 5.7 ± 0.69
4.957ThrVal: 4.957 ± 0.868
0.558ThrTrp: 0.558 ± 0.259
1.735ThrTyr: 1.735 ± 0.29
0.0ThrXaa: 0.0 ± 0.0
Val
7.807ValAla: 7.807 ± 0.638
0.124ValCys: 0.124 ± 0.095
6.382ValAsp: 6.382 ± 0.607
5.452ValGlu: 5.452 ± 0.637
2.169ValPhe: 2.169 ± 0.294
5.019ValGly: 5.019 ± 0.657
1.053ValHis: 1.053 ± 0.28
4.461ValIle: 4.461 ± 0.584
3.346ValLys: 3.346 ± 0.43
5.019ValLeu: 5.019 ± 0.604
1.735ValMet: 1.735 ± 0.36
2.85ValAsn: 2.85 ± 0.472
3.594ValPro: 3.594 ± 0.416
3.098ValGln: 3.098 ± 0.424
5.452ValArg: 5.452 ± 0.587
4.027ValSer: 4.027 ± 0.569
5.204ValThr: 5.204 ± 0.546
6.877ValVal: 6.877 ± 0.777
1.301ValTrp: 1.301 ± 0.251
1.611ValTyr: 1.611 ± 0.273
0.0ValXaa: 0.0 ± 0.0
Trp
1.611TrpAla: 1.611 ± 0.33
0.124TrpCys: 0.124 ± 0.088
1.239TrpAsp: 1.239 ± 0.314
0.991TrpGlu: 0.991 ± 0.278
0.743TrpPhe: 0.743 ± 0.275
0.929TrpGly: 0.929 ± 0.25
0.434TrpHis: 0.434 ± 0.177
0.682TrpIle: 0.682 ± 0.223
1.115TrpLys: 1.115 ± 0.226
1.859TrpLeu: 1.859 ± 0.327
0.31TrpMet: 0.31 ± 0.167
0.496TrpAsn: 0.496 ± 0.133
0.743TrpPro: 0.743 ± 0.212
0.434TrpGln: 0.434 ± 0.171
1.363TrpArg: 1.363 ± 0.273
0.558TrpSer: 0.558 ± 0.181
0.805TrpThr: 0.805 ± 0.233
1.177TrpVal: 1.177 ± 0.209
0.186TrpTrp: 0.186 ± 0.109
0.434TrpTyr: 0.434 ± 0.191
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.983TyrAla: 1.983 ± 0.293
0.124TyrCys: 0.124 ± 0.095
1.363TyrAsp: 1.363 ± 0.241
1.301TyrGlu: 1.301 ± 0.291
0.743TyrPhe: 0.743 ± 0.214
2.664TyrGly: 2.664 ± 0.409
0.186TyrHis: 0.186 ± 0.102
0.805TyrIle: 0.805 ± 0.241
0.991TyrLys: 0.991 ± 0.252
1.239TyrLeu: 1.239 ± 0.275
0.805TyrMet: 0.805 ± 0.222
1.239TyrAsn: 1.239 ± 0.242
1.053TyrPro: 1.053 ± 0.29
0.867TyrGln: 0.867 ± 0.214
1.301TyrArg: 1.301 ± 0.257
2.169TyrSer: 2.169 ± 0.403
1.115TyrThr: 1.115 ± 0.267
2.788TyrVal: 2.788 ± 0.487
0.31TyrTrp: 0.31 ± 0.138
0.434TyrTyr: 0.434 ± 0.179
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 79 proteins (16141 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski