Amycolatopsis alkalitolerans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Amycolatopsis

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6455 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C4LZD7|A0A5C4LZD7_9PSEU PadR family transcriptional regulator OS=Amycolatopsis alkalitolerans OX=2547244 GN=FG385_22210 PE=4 SV=1
MM1 pKa = 7.16SQPIQTLHH9 pKa = 6.18EE10 pKa = 4.48FVFNLISNADD20 pKa = 3.57FGSAFLSDD28 pKa = 3.66PASALSAAGLGDD40 pKa = 3.21ITGADD45 pKa = 3.7VQEE48 pKa = 4.26VTPLVTDD55 pKa = 3.63SMPAPVADD63 pKa = 3.55AVEE66 pKa = 4.62SGLASLPTDD75 pKa = 3.82AFGTDD80 pKa = 4.18DD81 pKa = 3.74LHH83 pKa = 7.42CALTHH88 pKa = 6.48LEE90 pKa = 4.36TVASVAQSLPDD101 pKa = 3.7TALSTAGTLSSVTDD115 pKa = 3.43AMGVSGTFQGSADD128 pKa = 3.61GLATGMLASTPAGDD142 pKa = 3.57VTGALAATTGSLGAGMEE159 pKa = 4.56SPLGTYY165 pKa = 10.44GITTDD170 pKa = 4.05SLPLSVPSFGSVSDD184 pKa = 3.97LGGTLDD190 pKa = 3.99SAALTGGTPVTEE202 pKa = 4.29TATSYY207 pKa = 8.58VTSGTEE213 pKa = 3.65MATSGIANGSAMLGDD228 pKa = 5.21HH229 pKa = 7.08ISTAGTGLGGAVTAGGADD247 pKa = 3.87LANHH251 pKa = 5.14VTSAVSMAGDD261 pKa = 3.89LTSNLPTVSAPVHH274 pKa = 6.33LPADD278 pKa = 3.95LPVHH282 pKa = 6.46VVAQPPALPAADD294 pKa = 3.38AAVPQVTHH302 pKa = 5.45TVEE305 pKa = 4.3STVSHH310 pKa = 6.73ASVLPDD316 pKa = 3.6SLSHH320 pKa = 6.94GSLAGMAHH328 pKa = 6.02SAVPGVDD335 pKa = 3.41SLHH338 pKa = 6.5TDD340 pKa = 4.02LLHH343 pKa = 7.34GEE345 pKa = 4.57LPLGHH350 pKa = 7.14

Molecular weight: 33.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C4M5P3|A0A5C4M5P3_9PSEU BldC family transcriptional regulator OS=Amycolatopsis alkalitolerans OX=2547244 GN=FG385_04725 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.72HH17 pKa = 5.63RR18 pKa = 11.84KK19 pKa = 7.68LLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84MQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 9.69QGKK33 pKa = 8.53

Molecular weight: 4.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6455

0

6455

2018279

27

2075

312.7

33.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.215 ± 0.045

0.793 ± 0.01

5.663 ± 0.024

5.775 ± 0.028

2.97 ± 0.019

9.339 ± 0.03

2.237 ± 0.014

3.696 ± 0.019

2.13 ± 0.02

10.581 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.778 ± 0.012

1.912 ± 0.018

5.798 ± 0.025

2.799 ± 0.016

8.004 ± 0.035

5.083 ± 0.021

5.814 ± 0.024

8.873 ± 0.025

1.478 ± 0.013

2.064 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski