Pontibacter fetidus

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Hymenobacteraceae; Pontibacter

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3463 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B2H9T1|A0A6B2H9T1_9BACT Sigma-54-dependent Fis family transcriptional regulator OS=Pontibacter fetidus OX=2700082 GN=GWO68_13720 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 10.46NFNNYY7 pKa = 6.9TSRR10 pKa = 11.84NSSILSKK17 pKa = 10.85QLLCSLLFGLLVFMPAVLHH36 pKa = 6.31ANYY39 pKa = 10.26VSLNKK44 pKa = 8.32TTEE47 pKa = 3.48IRR49 pKa = 11.84NTLLEE54 pKa = 4.0FDD56 pKa = 3.95NNKK59 pKa = 9.78VKK61 pKa = 10.79DD62 pKa = 4.07ILWSAIGITNDD73 pKa = 2.71VDD75 pKa = 3.64YY76 pKa = 11.47HH77 pKa = 6.55KK78 pKa = 10.65VTEE81 pKa = 4.14AKK83 pKa = 10.28FDD85 pKa = 3.83GGDD88 pKa = 3.38FSLDD92 pKa = 3.5FVASSPNTYY101 pKa = 10.57DD102 pKa = 3.02HH103 pKa = 6.36STGGGAFDD111 pKa = 4.11TRR113 pKa = 11.84DD114 pKa = 3.22VGQDD118 pKa = 2.91TGDD121 pKa = 4.76DD122 pKa = 3.25IVEE125 pKa = 4.25SLEE128 pKa = 4.89GGDD131 pKa = 4.03FTCGDD136 pKa = 3.18IVTFLTQIVVDD147 pKa = 4.14KK148 pKa = 10.86DD149 pKa = 3.57AVGSQTIDD157 pKa = 3.13LDD159 pKa = 4.16YY160 pKa = 11.49EE161 pKa = 4.22FLADD165 pKa = 3.49ATGQSGVALADD176 pKa = 3.29IVNVQINYY184 pKa = 9.49GAVSGGDD191 pKa = 3.66GVGSTDD197 pKa = 5.02SGIKK201 pKa = 10.16DD202 pKa = 3.68DD203 pKa = 4.62GGSVATLSNEE213 pKa = 4.2HH214 pKa = 6.02ITGDD218 pKa = 3.84GEE220 pKa = 4.64LFNDD224 pKa = 3.58NKK226 pKa = 10.95LVGTVTITDD235 pKa = 4.26LEE237 pKa = 4.21ASEE240 pKa = 4.18EE241 pKa = 4.41VIVRR245 pKa = 11.84VDD247 pKa = 3.07VRR249 pKa = 11.84IACDD253 pKa = 3.59PTSSPTGNLQAAITAALVIDD273 pKa = 4.84PEE275 pKa = 5.39DD276 pKa = 3.83DD277 pKa = 3.96VISVGNQTVPFKK289 pKa = 10.91NVNQIIFPTCAITPATAVCEE309 pKa = 4.07LATTSYY315 pKa = 9.61TASSDD320 pKa = 3.57VADD323 pKa = 3.92ATFVWSITGDD333 pKa = 3.58GTIVDD338 pKa = 4.11AASDD342 pKa = 3.77GTKK345 pKa = 10.14TIVASGGATSTSVSVLAGSAGSYY368 pKa = 8.33TLSVSISKK376 pKa = 8.97TGFGTQTCSQEE387 pKa = 3.56ITVNDD392 pKa = 4.01VPTVTAGADD401 pKa = 3.4QTVCAASPIQTLTATATVSTGATLVWYY428 pKa = 9.33DD429 pKa = 3.61AASGGNTVANPTLSSIGTATYY450 pKa = 9.77YY451 pKa = 10.99AQANLGDD458 pKa = 4.28CSSGRR463 pKa = 11.84VPVVLTINEE472 pKa = 4.54TPTVTAGADD481 pKa = 3.4QTVCAASPIQTLTATATVSTGATLVWYY508 pKa = 9.33DD509 pKa = 3.65AASGGNN515 pKa = 3.39

Molecular weight: 52.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B2H6F0|A0A6B2H6F0_9BACT Metal ABC transporter ATP-binding protein OS=Pontibacter fetidus OX=2700082 GN=GWO68_05890 PE=4 SV=1
MM1 pKa = 7.29CTFATRR7 pKa = 11.84SAGRR11 pKa = 11.84EE12 pKa = 3.54EE13 pKa = 4.32RR14 pKa = 11.84KK15 pKa = 9.7RR16 pKa = 11.84GRR18 pKa = 11.84SGAQEE23 pKa = 4.05TCLHH27 pKa = 6.8FIKK30 pKa = 10.71LVLRR34 pKa = 11.84LVSTAKK40 pKa = 10.14RR41 pKa = 11.84SKK43 pKa = 10.89NYY45 pKa = 10.22FLTRR49 pKa = 11.84LQKK52 pKa = 10.22QKK54 pKa = 11.02QKK56 pKa = 11.2VYY58 pKa = 10.95LCTPNAAAGRR68 pKa = 11.84AGKK71 pKa = 9.41EE72 pKa = 3.79AEE74 pKa = 4.2TQGFFSGFLSGLTPEE89 pKa = 4.47KK90 pKa = 10.59RR91 pKa = 11.84GG92 pKa = 3.47

Molecular weight: 10.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3463

0

3463

1154467

38

3618

333.4

37.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.101 ± 0.048

0.741 ± 0.014

5.129 ± 0.034

6.269 ± 0.052

4.45 ± 0.032

6.793 ± 0.041

1.96 ± 0.02

6.469 ± 0.038

6.38 ± 0.051

9.927 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.349 ± 0.021

5.025 ± 0.032

4.034 ± 0.027

4.134 ± 0.027

4.252 ± 0.031

5.77 ± 0.035

6.263 ± 0.063

6.79 ± 0.038

1.086 ± 0.014

4.077 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski