Amino acid dipepetide frequency for Mycobacterium phage RedRock

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.117AlaAla: 11.117 ± 1.172
0.672AlaCys: 0.672 ± 0.181
5.436AlaAsp: 5.436 ± 0.609
7.758AlaGlu: 7.758 ± 0.773
4.032AlaPhe: 4.032 ± 0.546
7.758AlaGly: 7.758 ± 0.755
1.466AlaHis: 1.466 ± 0.254
4.337AlaIle: 4.337 ± 0.507
5.07AlaLys: 5.07 ± 0.589
8.796AlaLeu: 8.796 ± 0.934
3.543AlaMet: 3.543 ± 0.543
3.36AlaAsn: 3.36 ± 0.477
4.093AlaPro: 4.093 ± 0.622
4.032AlaGln: 4.032 ± 0.552
6.78AlaArg: 6.78 ± 0.694
4.948AlaSer: 4.948 ± 0.61
5.864AlaThr: 5.864 ± 0.618
7.88AlaVal: 7.88 ± 0.682
1.71AlaTrp: 1.71 ± 0.357
2.627AlaTyr: 2.627 ± 0.455
0.0AlaXaa: 0.0 ± 0.0
Cys
0.55CysAla: 0.55 ± 0.216
0.0CysCys: 0.0 ± 0.0
0.367CysAsp: 0.367 ± 0.167
0.55CysGlu: 0.55 ± 0.179
0.305CysPhe: 0.305 ± 0.162
0.672CysGly: 0.672 ± 0.207
0.244CysHis: 0.244 ± 0.158
0.428CysIle: 0.428 ± 0.218
0.367CysLys: 0.367 ± 0.135
0.794CysLeu: 0.794 ± 0.233
0.122CysMet: 0.122 ± 0.082
0.428CysAsn: 0.428 ± 0.148
0.367CysPro: 0.367 ± 0.212
0.061CysGln: 0.061 ± 0.059
0.55CysArg: 0.55 ± 0.187
0.428CysSer: 0.428 ± 0.144
0.733CysThr: 0.733 ± 0.226
0.489CysVal: 0.489 ± 0.152
0.305CysTrp: 0.305 ± 0.145
0.305CysTyr: 0.305 ± 0.133
0.0CysXaa: 0.0 ± 0.0
Asp
6.658AspAla: 6.658 ± 0.665
0.672AspCys: 0.672 ± 0.254
4.093AspAsp: 4.093 ± 0.589
4.093AspGlu: 4.093 ± 0.552
2.688AspPhe: 2.688 ± 0.461
5.559AspGly: 5.559 ± 0.686
1.649AspHis: 1.649 ± 0.358
3.726AspIle: 3.726 ± 0.463
2.016AspLys: 2.016 ± 0.37
5.864AspLeu: 5.864 ± 0.786
1.71AspMet: 1.71 ± 0.309
1.588AspAsn: 1.588 ± 0.283
4.703AspPro: 4.703 ± 0.649
2.138AspGln: 2.138 ± 0.31
3.115AspArg: 3.115 ± 0.471
2.81AspSer: 2.81 ± 0.505
3.787AspThr: 3.787 ± 0.56
4.032AspVal: 4.032 ± 0.473
1.283AspTrp: 1.283 ± 0.283
2.321AspTyr: 2.321 ± 0.324
0.0AspXaa: 0.0 ± 0.0
Glu
8.002GluAla: 8.002 ± 0.736
0.183GluCys: 0.183 ± 0.102
5.253GluAsp: 5.253 ± 0.705
4.948GluGlu: 4.948 ± 0.737
3.237GluPhe: 3.237 ± 0.475
4.642GluGly: 4.642 ± 0.542
1.283GluHis: 1.283 ± 0.307
3.909GluIle: 3.909 ± 0.409
2.443GluLys: 2.443 ± 0.34
6.902GluLeu: 6.902 ± 0.795
2.321GluMet: 2.321 ± 0.322
2.016GluAsn: 2.016 ± 0.343
2.81GluPro: 2.81 ± 0.397
2.321GluGln: 2.321 ± 0.366
4.642GluArg: 4.642 ± 0.622
2.81GluSer: 2.81 ± 0.468
3.604GluThr: 3.604 ± 0.519
5.314GluVal: 5.314 ± 0.687
1.405GluTrp: 1.405 ± 0.244
1.71GluTyr: 1.71 ± 0.332
0.0GluXaa: 0.0 ± 0.0
Phe
2.993PheAla: 2.993 ± 0.478
0.428PheCys: 0.428 ± 0.18
2.504PheAsp: 2.504 ± 0.363
3.299PheGlu: 3.299 ± 0.405
0.794PhePhe: 0.794 ± 0.252
3.299PheGly: 3.299 ± 0.461
0.916PheHis: 0.916 ± 0.297
1.71PheIle: 1.71 ± 0.37
1.527PheLys: 1.527 ± 0.337
2.688PheLeu: 2.688 ± 0.54
0.55PheMet: 0.55 ± 0.156
1.649PheAsn: 1.649 ± 0.336
2.26PhePro: 2.26 ± 0.417
0.916PheGln: 0.916 ± 0.213
2.138PheArg: 2.138 ± 0.354
2.016PheSer: 2.016 ± 0.324
2.26PheThr: 2.26 ± 0.372
2.321PheVal: 2.321 ± 0.325
0.428PheTrp: 0.428 ± 0.143
0.733PheTyr: 0.733 ± 0.201
0.0PheXaa: 0.0 ± 0.0
Gly
6.841GlyAla: 6.841 ± 0.886
0.611GlyCys: 0.611 ± 0.191
6.475GlyAsp: 6.475 ± 1.04
5.192GlyGlu: 5.192 ± 0.722
3.176GlyPhe: 3.176 ± 0.499
7.391GlyGly: 7.391 ± 1.287
1.222GlyHis: 1.222 ± 0.3
4.276GlyIle: 4.276 ± 0.588
3.848GlyLys: 3.848 ± 0.462
6.292GlyLeu: 6.292 ± 0.706
2.016GlyMet: 2.016 ± 0.325
3.665GlyAsn: 3.665 ± 0.799
5.498GlyPro: 5.498 ± 2.036
3.299GlyGln: 3.299 ± 0.409
4.093GlyArg: 4.093 ± 0.483
3.604GlySer: 3.604 ± 0.593
5.436GlyThr: 5.436 ± 0.685
6.597GlyVal: 6.597 ± 0.639
0.977GlyTrp: 0.977 ± 0.259
2.382GlyTyr: 2.382 ± 0.326
0.0GlyXaa: 0.0 ± 0.0
His
1.405HisAla: 1.405 ± 0.289
0.305HisCys: 0.305 ± 0.129
1.344HisAsp: 1.344 ± 0.322
1.588HisGlu: 1.588 ± 0.36
0.55HisPhe: 0.55 ± 0.179
1.649HisGly: 1.649 ± 0.403
0.428HisHis: 0.428 ± 0.174
0.916HisIle: 0.916 ± 0.22
1.038HisLys: 1.038 ± 0.255
1.405HisLeu: 1.405 ± 0.281
0.367HisMet: 0.367 ± 0.193
0.672HisAsn: 0.672 ± 0.214
1.1HisPro: 1.1 ± 0.257
0.733HisGln: 0.733 ± 0.224
1.527HisArg: 1.527 ± 0.368
0.977HisSer: 0.977 ± 0.25
0.855HisThr: 0.855 ± 0.199
1.1HisVal: 1.1 ± 0.292
0.428HisTrp: 0.428 ± 0.172
0.611HisTyr: 0.611 ± 0.287
0.0HisXaa: 0.0 ± 0.0
Ile
5.986IleAla: 5.986 ± 0.597
0.55IleCys: 0.55 ± 0.207
3.97IleAsp: 3.97 ± 0.445
4.581IleGlu: 4.581 ± 0.492
1.283IlePhe: 1.283 ± 0.244
4.765IleGly: 4.765 ± 0.569
1.038IleHis: 1.038 ± 0.251
1.771IleIle: 1.771 ± 0.331
2.199IleLys: 2.199 ± 0.38
3.787IleLeu: 3.787 ± 0.432
0.611IleMet: 0.611 ± 0.196
2.321IleAsn: 2.321 ± 0.322
3.054IlePro: 3.054 ± 0.352
1.283IleGln: 1.283 ± 0.289
2.932IleArg: 2.932 ± 0.427
2.26IleSer: 2.26 ± 0.355
2.932IleThr: 2.932 ± 0.354
2.993IleVal: 2.993 ± 0.528
0.428IleTrp: 0.428 ± 0.151
0.916IleTyr: 0.916 ± 0.222
0.0IleXaa: 0.0 ± 0.0
Lys
5.253LysAla: 5.253 ± 0.63
0.244LysCys: 0.244 ± 0.166
2.077LysAsp: 2.077 ± 0.352
1.71LysGlu: 1.71 ± 0.354
1.222LysPhe: 1.222 ± 0.256
4.154LysGly: 4.154 ± 0.68
0.367LysHis: 0.367 ± 0.139
1.894LysIle: 1.894 ± 0.312
2.993LysLys: 2.993 ± 0.537
3.604LysLeu: 3.604 ± 0.502
1.038LysMet: 1.038 ± 0.255
1.466LysAsn: 1.466 ± 0.307
2.81LysPro: 2.81 ± 0.495
1.1LysGln: 1.1 ± 0.22
3.787LysArg: 3.787 ± 0.603
1.955LysSer: 1.955 ± 0.455
2.871LysThr: 2.871 ± 0.488
3.604LysVal: 3.604 ± 0.455
0.733LysTrp: 0.733 ± 0.222
1.466LysTyr: 1.466 ± 0.3
0.0LysXaa: 0.0 ± 0.0
Leu
9.163LeuAla: 9.163 ± 0.824
0.611LeuCys: 0.611 ± 0.199
4.887LeuAsp: 4.887 ± 0.537
6.353LeuGlu: 6.353 ± 0.58
2.199LeuPhe: 2.199 ± 0.335
5.498LeuGly: 5.498 ± 0.622
1.894LeuHis: 1.894 ± 0.368
4.276LeuIle: 4.276 ± 0.601
3.115LeuLys: 3.115 ± 0.489
5.375LeuLeu: 5.375 ± 0.658
2.443LeuMet: 2.443 ± 0.488
2.138LeuAsn: 2.138 ± 0.405
5.07LeuPro: 5.07 ± 0.543
3.36LeuGln: 3.36 ± 0.512
5.986LeuArg: 5.986 ± 0.737
5.375LeuSer: 5.375 ± 0.57
5.436LeuThr: 5.436 ± 0.74
4.093LeuVal: 4.093 ± 0.459
1.71LeuTrp: 1.71 ± 0.325
2.382LeuTyr: 2.382 ± 0.379
0.0LeuXaa: 0.0 ± 0.0
Met
2.627MetAla: 2.627 ± 0.4
0.061MetCys: 0.061 ± 0.059
1.283MetAsp: 1.283 ± 0.35
1.405MetGlu: 1.405 ± 0.315
0.794MetPhe: 0.794 ± 0.201
1.527MetGly: 1.527 ± 0.264
0.489MetHis: 0.489 ± 0.191
1.405MetIle: 1.405 ± 0.298
1.527MetLys: 1.527 ± 0.304
1.649MetLeu: 1.649 ± 0.342
0.367MetMet: 0.367 ± 0.138
0.55MetAsn: 0.55 ± 0.196
1.344MetPro: 1.344 ± 0.339
1.038MetGln: 1.038 ± 0.316
1.466MetArg: 1.466 ± 0.246
2.749MetSer: 2.749 ± 0.315
2.81MetThr: 2.81 ± 0.381
1.405MetVal: 1.405 ± 0.305
0.305MetTrp: 0.305 ± 0.147
0.855MetTyr: 0.855 ± 0.202
0.0MetXaa: 0.0 ± 0.0
Asn
3.237AsnAla: 3.237 ± 0.453
0.489AsnCys: 0.489 ± 0.152
2.016AsnAsp: 2.016 ± 0.288
2.382AsnGlu: 2.382 ± 0.394
0.794AsnPhe: 0.794 ± 0.287
3.787AsnGly: 3.787 ± 0.672
0.977AsnHis: 0.977 ± 0.234
1.344AsnIle: 1.344 ± 0.3
0.916AsnLys: 0.916 ± 0.205
2.932AsnLeu: 2.932 ± 0.465
0.855AsnMet: 0.855 ± 0.259
0.55AsnAsn: 0.55 ± 0.204
2.688AsnPro: 2.688 ± 0.388
0.611AsnGln: 0.611 ± 0.192
2.016AsnArg: 2.016 ± 0.399
1.405AsnSer: 1.405 ± 0.3
1.833AsnThr: 1.833 ± 0.361
2.504AsnVal: 2.504 ± 0.361
0.977AsnTrp: 0.977 ± 0.209
0.672AsnTyr: 0.672 ± 0.182
0.0AsnXaa: 0.0 ± 0.0
Pro
5.681ProAla: 5.681 ± 0.608
0.367ProCys: 0.367 ± 0.146
4.215ProAsp: 4.215 ± 0.642
5.009ProGlu: 5.009 ± 0.6
1.649ProPhe: 1.649 ± 0.341
4.52ProGly: 4.52 ± 0.685
1.161ProHis: 1.161 ± 0.261
2.627ProIle: 2.627 ± 0.427
2.627ProLys: 2.627 ± 0.606
3.054ProLeu: 3.054 ± 0.542
1.161ProMet: 1.161 ± 0.307
2.321ProAsn: 2.321 ± 0.36
2.993ProPro: 2.993 ± 0.546
2.566ProGln: 2.566 ± 0.767
3.787ProArg: 3.787 ± 0.603
2.627ProSer: 2.627 ± 0.383
3.115ProThr: 3.115 ± 0.427
3.848ProVal: 3.848 ± 0.33
1.038ProTrp: 1.038 ± 0.346
1.405ProTyr: 1.405 ± 0.312
0.0ProXaa: 0.0 ± 0.0
Gln
4.093GlnAla: 4.093 ± 0.608
0.244GlnCys: 0.244 ± 0.139
1.161GlnAsp: 1.161 ± 0.252
2.016GlnGlu: 2.016 ± 0.393
1.771GlnPhe: 1.771 ± 0.426
4.581GlnGly: 4.581 ± 1.505
0.672GlnHis: 0.672 ± 0.188
2.81GlnIle: 2.81 ± 0.374
1.466GlnLys: 1.466 ± 0.325
2.993GlnLeu: 2.993 ± 0.633
0.977GlnMet: 0.977 ± 0.217
0.794GlnAsn: 0.794 ± 0.228
1.405GlnPro: 1.405 ± 0.278
1.955GlnGln: 1.955 ± 0.363
1.955GlnArg: 1.955 ± 0.483
1.588GlnSer: 1.588 ± 0.273
2.077GlnThr: 2.077 ± 0.347
2.382GlnVal: 2.382 ± 0.422
0.916GlnTrp: 0.916 ± 0.246
0.916GlnTyr: 0.916 ± 0.203
0.0GlnXaa: 0.0 ± 0.0
Arg
5.62ArgAla: 5.62 ± 0.74
0.916ArgCys: 0.916 ± 0.263
4.276ArgAsp: 4.276 ± 0.472
5.314ArgGlu: 5.314 ± 0.882
2.566ArgPhe: 2.566 ± 0.445
4.276ArgGly: 4.276 ± 0.686
1.344ArgHis: 1.344 ± 0.296
3.604ArgIle: 3.604 ± 0.453
2.871ArgLys: 2.871 ± 0.541
5.864ArgLeu: 5.864 ± 0.676
1.71ArgMet: 1.71 ± 0.287
1.833ArgAsn: 1.833 ± 0.294
2.81ArgPro: 2.81 ± 0.46
2.321ArgGln: 2.321 ± 0.411
4.948ArgArg: 4.948 ± 0.606
2.688ArgSer: 2.688 ± 0.421
2.932ArgThr: 2.932 ± 0.47
4.581ArgVal: 4.581 ± 0.552
1.222ArgTrp: 1.222 ± 0.323
1.894ArgTyr: 1.894 ± 0.354
0.0ArgXaa: 0.0 ± 0.0
Ser
4.459SerAla: 4.459 ± 0.525
0.367SerCys: 0.367 ± 0.158
2.566SerAsp: 2.566 ± 0.33
3.115SerGlu: 3.115 ± 0.511
2.321SerPhe: 2.321 ± 0.351
4.398SerGly: 4.398 ± 0.658
0.733SerHis: 0.733 ± 0.207
2.504SerIle: 2.504 ± 0.306
2.627SerLys: 2.627 ± 0.47
4.337SerLeu: 4.337 ± 0.586
1.344SerMet: 1.344 ± 0.262
1.283SerAsn: 1.283 ± 0.253
2.81SerPro: 2.81 ± 0.336
2.26SerGln: 2.26 ± 0.361
3.421SerArg: 3.421 ± 0.508
2.688SerSer: 2.688 ± 0.438
3.115SerThr: 3.115 ± 0.408
3.237SerVal: 3.237 ± 0.505
1.038SerTrp: 1.038 ± 0.259
1.771SerTyr: 1.771 ± 0.349
0.0SerXaa: 0.0 ± 0.0
Thr
7.025ThrAla: 7.025 ± 0.702
0.367ThrCys: 0.367 ± 0.15
3.604ThrAsp: 3.604 ± 0.477
3.36ThrGlu: 3.36 ± 0.46
2.26ThrPhe: 2.26 ± 0.346
5.864ThrGly: 5.864 ± 0.906
0.916ThrHis: 0.916 ± 0.226
2.443ThrIle: 2.443 ± 0.435
2.993ThrLys: 2.993 ± 0.456
5.131ThrLeu: 5.131 ± 0.65
1.649ThrMet: 1.649 ± 0.344
2.016ThrAsn: 2.016 ± 0.322
4.642ThrPro: 4.642 ± 0.553
2.26ThrGln: 2.26 ± 0.37
2.871ThrArg: 2.871 ± 0.432
2.749ThrSer: 2.749 ± 0.479
3.726ThrThr: 3.726 ± 0.547
3.909ThrVal: 3.909 ± 0.508
1.405ThrTrp: 1.405 ± 0.338
2.077ThrTyr: 2.077 ± 0.335
0.0ThrXaa: 0.0 ± 0.0
Val
6.047ValAla: 6.047 ± 0.608
0.55ValCys: 0.55 ± 0.196
5.498ValAsp: 5.498 ± 0.586
3.787ValGlu: 3.787 ± 0.5
2.443ValPhe: 2.443 ± 0.45
5.192ValGly: 5.192 ± 0.597
1.222ValHis: 1.222 ± 0.29
3.176ValIle: 3.176 ± 0.575
3.421ValLys: 3.421 ± 0.4
6.414ValLeu: 6.414 ± 0.723
1.649ValMet: 1.649 ± 0.366
2.26ValAsn: 2.26 ± 0.373
3.054ValPro: 3.054 ± 0.431
2.443ValGln: 2.443 ± 0.383
4.215ValArg: 4.215 ± 0.514
4.032ValSer: 4.032 ± 0.514
4.948ValThr: 4.948 ± 0.577
5.742ValVal: 5.742 ± 0.662
1.649ValTrp: 1.649 ± 0.36
2.138ValTyr: 2.138 ± 0.434
0.0ValXaa: 0.0 ± 0.0
Trp
1.649TrpAla: 1.649 ± 0.364
0.244TrpCys: 0.244 ± 0.14
1.1TrpAsp: 1.1 ± 0.246
1.283TrpGlu: 1.283 ± 0.307
0.55TrpPhe: 0.55 ± 0.186
1.344TrpGly: 1.344 ± 0.282
0.611TrpHis: 0.611 ± 0.236
1.038TrpIle: 1.038 ± 0.281
0.55TrpLys: 0.55 ± 0.201
1.038TrpLeu: 1.038 ± 0.252
0.489TrpMet: 0.489 ± 0.169
0.977TrpAsn: 0.977 ± 0.32
0.916TrpPro: 0.916 ± 0.22
1.222TrpGln: 1.222 ± 0.258
1.283TrpArg: 1.283 ± 0.271
1.038TrpSer: 1.038 ± 0.287
1.038TrpThr: 1.038 ± 0.194
1.466TrpVal: 1.466 ± 0.294
0.55TrpTrp: 0.55 ± 0.212
0.672TrpTyr: 0.672 ± 0.212
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.932TyrAla: 2.932 ± 0.363
0.183TyrCys: 0.183 ± 0.101
2.382TyrAsp: 2.382 ± 0.328
2.016TyrGlu: 2.016 ± 0.35
0.794TyrPhe: 0.794 ± 0.207
2.016TyrGly: 2.016 ± 0.364
0.367TyrHis: 0.367 ± 0.163
1.466TyrIle: 1.466 ± 0.254
0.733TyrLys: 0.733 ± 0.196
2.443TyrLeu: 2.443 ± 0.334
0.672TyrMet: 0.672 ± 0.207
1.1TyrAsn: 1.1 ± 0.245
1.344TyrPro: 1.344 ± 0.278
0.855TyrGln: 0.855 ± 0.2
2.077TyrArg: 2.077 ± 0.38
1.649TyrSer: 1.649 ± 0.313
1.894TyrThr: 1.894 ± 0.359
2.382TyrVal: 2.382 ± 0.41
0.55TyrTrp: 0.55 ± 0.228
0.489TyrTyr: 0.489 ± 0.174
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 95 proteins (16372 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski