Synechococcus phage S-CRM01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 297 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F5B3Z5|F5B3Z5_9CAUD Uncharacterized protein OS=Synechococcus phage S-CRM01 OX=1026955 GN=SCRM01_125c PE=4 SV=1
MM1 pKa = 8.08AYY3 pKa = 9.73PIDD6 pKa = 4.4PNSAAFAPYY15 pKa = 9.95VGSLSTTNPVADD27 pKa = 3.94SDD29 pKa = 4.14DD30 pKa = 5.02LYY32 pKa = 11.37VSSVGIAIRR41 pKa = 11.84RR42 pKa = 11.84WKK44 pKa = 10.59DD45 pKa = 3.06KK46 pKa = 11.08AGTRR50 pKa = 11.84FWDD53 pKa = 3.48EE54 pKa = 3.77LLVPFEE60 pKa = 4.33VQTPPSVYY68 pKa = 10.1TDD70 pKa = 3.14ATAPLGGTGPQYY82 pKa = 10.62PVYY85 pKa = 10.55NPVLGGQVTVAVAPGSVAEE104 pKa = 5.0DD105 pKa = 3.63GAPNLVFTFSRR116 pKa = 11.84NTSSRR121 pKa = 11.84NVLTVNYY128 pKa = 9.32NITGTATNGVDD139 pKa = 3.51YY140 pKa = 8.46EE141 pKa = 4.64TIGNSVTFAEE151 pKa = 5.3GAATATVVVNPIADD165 pKa = 3.39AGVEE169 pKa = 4.01PNEE172 pKa = 4.24TVILTIVPNGAYY184 pKa = 9.58TIGTPNSATGTITNDD199 pKa = 3.41DD200 pKa = 3.52AA201 pKa = 7.25

Molecular weight: 20.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F5B3Y3|F5B3Y3_9CAUD Uncharacterized protein OS=Synechococcus phage S-CRM01 OX=1026955 GN=SCRM01_113c PE=4 SV=1
MM1 pKa = 7.35LTNLLLTSSLFGVFGVSPKK20 pKa = 10.06VEE22 pKa = 4.02QPKK25 pKa = 9.74PVHH28 pKa = 5.15TVKK31 pKa = 10.45EE32 pKa = 4.38KK33 pKa = 10.74CSSASWYY40 pKa = 10.8GPGFHH45 pKa = 7.19GRR47 pKa = 11.84RR48 pKa = 11.84TANGEE53 pKa = 4.07VFHH56 pKa = 6.61QDD58 pKa = 2.99SLTAAHH64 pKa = 6.67KK65 pKa = 9.8HH66 pKa = 5.59LPFGTRR72 pKa = 11.84VRR74 pKa = 11.84VTNRR78 pKa = 11.84HH79 pKa = 5.03NGKK82 pKa = 10.36SIIVRR87 pKa = 11.84INDD90 pKa = 3.58RR91 pKa = 11.84GPFIGGRR98 pKa = 11.84ALDD101 pKa = 4.1LSRR104 pKa = 11.84EE105 pKa = 3.86AAARR109 pKa = 11.84IGMIRR114 pKa = 11.84SGHH117 pKa = 6.18APVCYY122 pKa = 10.38SEE124 pKa = 4.24VV125 pKa = 3.19

Molecular weight: 13.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

297

0

297

53448

29

2484

180.0

20.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.671 ± 0.165

1.05 ± 0.071

6.446 ± 0.144

6.87 ± 0.208

4.3 ± 0.104

6.968 ± 0.3

1.667 ± 0.097

6.593 ± 0.169

6.185 ± 0.272

7.899 ± 0.141

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.281 ± 0.113

5.886 ± 0.12

4.61 ± 0.142

3.955 ± 0.163

4.363 ± 0.097

6.767 ± 0.178

6.447 ± 0.188

6.318 ± 0.167

1.465 ± 0.071

4.253 ± 0.115
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski