Tomato yellow leaf curl virus (strain Israel) (TYLCV)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Tomato yellow leaf curl virus

Average proteome isoelectric point is 7.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P27262|TRAP_TYLCI Transcriptional activator protein OS=Tomato yellow leaf curl virus (strain Israel) OX=66366 GN=C2 PE=1 SV=1
MM1 pKa = 7.53PRR3 pKa = 11.84LFKK6 pKa = 10.34IYY8 pKa = 10.46AKK10 pKa = 10.51NYY12 pKa = 8.11FLTYY16 pKa = 9.42PNCSLSKK23 pKa = 10.98EE24 pKa = 4.17EE25 pKa = 5.68ALSQLKK31 pKa = 10.48KK32 pKa = 11.05LEE34 pKa = 4.06TPTNKK39 pKa = 10.15KK40 pKa = 9.54YY41 pKa = 10.82IKK43 pKa = 9.86VCKK46 pKa = 9.17EE47 pKa = 3.37LHH49 pKa = 6.47EE50 pKa = 4.83NGEE53 pKa = 4.16PHH55 pKa = 6.66LHH57 pKa = 6.03VLIQFEE63 pKa = 4.99GKK65 pKa = 8.83YY66 pKa = 8.65QCKK69 pKa = 8.66NQRR72 pKa = 11.84FFDD75 pKa = 3.91LVSPNRR81 pKa = 11.84SAHH84 pKa = 4.93FHH86 pKa = 6.59PNIQAAKK93 pKa = 10.07SSTDD97 pKa = 2.78VKK99 pKa = 10.53TYY101 pKa = 9.84VEE103 pKa = 4.13KK104 pKa = 10.99DD105 pKa = 3.15GNFIDD110 pKa = 5.22FGVSQIDD117 pKa = 3.59GRR119 pKa = 11.84SARR122 pKa = 11.84GGQQSANDD130 pKa = 4.01AYY132 pKa = 10.91AEE134 pKa = 4.09ALNSGSKK141 pKa = 10.29SEE143 pKa = 4.32ALNILKK149 pKa = 10.33EE150 pKa = 4.19KK151 pKa = 10.57APKK154 pKa = 10.17DD155 pKa = 3.79YY156 pKa = 10.76ILQFHH161 pKa = 6.52NLSSNLDD168 pKa = 4.01RR169 pKa = 11.84IFSPPLEE176 pKa = 4.43VYY178 pKa = 10.37VSPFLSSSFNQVPDD192 pKa = 3.83EE193 pKa = 4.21LEE195 pKa = 3.6EE196 pKa = 4.08WVAEE200 pKa = 4.03NVVYY204 pKa = 10.62SAARR208 pKa = 11.84PWRR211 pKa = 11.84PISIVIEE218 pKa = 4.23GDD220 pKa = 3.2SRR222 pKa = 11.84TGKK225 pKa = 8.52TMWARR230 pKa = 11.84SLGPHH235 pKa = 6.71NYY237 pKa = 10.18LCGHH241 pKa = 7.38LDD243 pKa = 4.18LSPKK247 pKa = 10.15VYY249 pKa = 11.07SNDD252 pKa = 2.25AWYY255 pKa = 10.92NVIDD259 pKa = 5.2DD260 pKa = 4.41VDD262 pKa = 3.61PHH264 pKa = 5.85YY265 pKa = 11.0LKK267 pKa = 10.7HH268 pKa = 6.2FKK270 pKa = 10.7EE271 pKa = 4.46FMGAQRR277 pKa = 11.84DD278 pKa = 3.81WQSNTKK284 pKa = 9.26YY285 pKa = 10.63GKK287 pKa = 9.49PIQIKK292 pKa = 10.42GGIPTIFLCNPGPTSSYY309 pKa = 10.83RR310 pKa = 11.84EE311 pKa = 3.96YY312 pKa = 11.26LDD314 pKa = 3.39EE315 pKa = 4.86EE316 pKa = 4.64KK317 pKa = 11.05NISLKK322 pKa = 10.6NWALKK327 pKa = 10.38NATFVTLYY335 pKa = 10.04EE336 pKa = 4.24PLFASINQGPTQDD349 pKa = 3.72SQEE352 pKa = 4.24EE353 pKa = 4.36TNKK356 pKa = 10.73AA357 pKa = 3.23

Molecular weight: 40.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P27271|AC4_TYLCI Protein C4 OS=Tomato yellow leaf curl virus (strain Israel) OX=66366 GN=C4 PE=3 SV=1
MM1 pKa = 7.57GNHH4 pKa = 6.64ISMCLSNSKK13 pKa = 11.01ANTNVRR19 pKa = 11.84TNGSSTWYY27 pKa = 8.75PQTGQHH33 pKa = 5.33ISIRR37 pKa = 11.84TFRR40 pKa = 11.84QLRR43 pKa = 11.84AQQMSRR49 pKa = 11.84PTWRR53 pKa = 11.84KK54 pKa = 7.56TEE56 pKa = 3.75TSLILEE62 pKa = 4.81FPKK65 pKa = 11.07SMADD69 pKa = 3.3QLEE72 pKa = 4.54EE73 pKa = 4.14VSNLPTTHH81 pKa = 6.13MPKK84 pKa = 10.37HH85 pKa = 6.65SIQAVNPRR93 pKa = 11.84PSII96 pKa = 3.76

Molecular weight: 10.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1098

96

357

183.0

21.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.645 ± 0.764

2.004 ± 0.431

4.827 ± 0.521

5.009 ± 0.843

4.736 ± 0.568

4.554 ± 0.417

4.189 ± 0.831

6.466 ± 0.75

6.466 ± 0.673

6.648 ± 1.004

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.459 ± 0.737

6.193 ± 0.449

5.92 ± 0.569

5.009 ± 0.658

6.466 ± 1.303

8.197 ± 1.013

5.1 ± 0.74

5.647 ± 0.84

1.366 ± 0.197

4.098 ± 0.59
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski