Bacillus phage Wip1

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes; Kalamavirales; Tectiviridae; Betatectivirus; Bacillus virus Wip1

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 27 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5Y6J0|S5Y6J0_9VIRU Uncharacterized protein OS=Bacillus phage Wip1 OX=663237 PE=4 SV=1
MM1 pKa = 7.94LDD3 pKa = 3.39QIKK6 pKa = 10.18VPNEE10 pKa = 3.8NKK12 pKa = 10.06GSKK15 pKa = 7.64QEE17 pKa = 3.94RR18 pKa = 11.84LQLVVDD24 pKa = 3.79QVVDD28 pKa = 3.74SLPTTVRR35 pKa = 11.84LLAGSYY41 pKa = 10.45LNSFRR46 pKa = 11.84QVLEE50 pKa = 4.45SEE52 pKa = 4.36QQDD55 pKa = 2.74IDD57 pKa = 4.26GNIDD61 pKa = 3.3NALSRR66 pKa = 11.84LRR68 pKa = 11.84EE69 pKa = 4.13YY70 pKa = 10.41IDD72 pKa = 3.81YY73 pKa = 10.24IQYY76 pKa = 10.89GHH78 pKa = 6.39DD79 pKa = 3.6QEE81 pKa = 5.88NEE83 pKa = 3.7

Molecular weight: 9.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5Z7E7|S5Z7E7_9VIRU Putative trimeric spike protein OS=Bacillus phage Wip1 OX=663237 PE=4 SV=1
MM1 pKa = 7.23GRR3 pKa = 11.84ILGIISGIGLLIALYY18 pKa = 10.59LFLSNARR25 pKa = 11.84QTTQIIDD32 pKa = 3.65SMAGNAVSGIKK43 pKa = 9.92VLQGRR48 pKa = 4.11

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

27

0

27

4405

47

898

163.1

18.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.607 ± 0.676

0.613 ± 0.218

5.766 ± 0.484

7.151 ± 0.813

4.858 ± 0.436

7.242 ± 0.778

1.362 ± 0.211

6.652 ± 0.691

8.876 ± 0.768

7.9 ± 0.492

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.951 ± 0.331

5.789 ± 0.53

3.814 ± 0.354

3.768 ± 0.461

4.336 ± 0.407

6.22 ± 0.421

5.766 ± 0.626

6.674 ± 0.624

1.112 ± 0.257

3.36 ± 0.386
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski