Polygala garcinii associated virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; unclassified Geminiviridae

Average proteome isoelectric point is 7.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I8B2Q1|A0A2I8B2Q1_9GEMI Movement protein OS=Polygala garcinii associated virus OX=2093274 PE=3 SV=1
MM1 pKa = 8.08WDD3 pKa = 3.75PLLHH7 pKa = 7.02DD8 pKa = 4.87FPEE11 pKa = 4.45SLHH14 pKa = 6.37GFRR17 pKa = 11.84CMLAVKK23 pKa = 10.28YY24 pKa = 8.24LLCVSSLYY32 pKa = 9.93PVQSVGFEE40 pKa = 4.19LLRR43 pKa = 11.84DD44 pKa = 4.31CISAVRR50 pKa = 11.84TRR52 pKa = 11.84SYY54 pKa = 11.77DD55 pKa = 3.46EE56 pKa = 4.79GSCKK60 pKa = 10.38YY61 pKa = 10.26SALCAKK67 pKa = 9.68VRR69 pKa = 11.84STSEE73 pKa = 3.95GEE75 pKa = 3.98LTQTRR80 pKa = 11.84TPLCCCPHH88 pKa = 6.83CPRR91 pKa = 11.84HH92 pKa = 5.72IGRR95 pKa = 11.84DD96 pKa = 3.31RR97 pKa = 11.84VEE99 pKa = 4.32TEE101 pKa = 3.99TKK103 pKa = 10.4EE104 pKa = 4.12PEE106 pKa = 3.93PP107 pKa = 4.53

Molecular weight: 12.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I8B2M1|A0A2I8B2M1_9GEMI Replication-associated protein OS=Polygala garcinii associated virus OX=2093274 PE=3 SV=1
MM1 pKa = 7.11TKK3 pKa = 10.3GRR5 pKa = 11.84VSIVPFVRR13 pKa = 11.84RR14 pKa = 11.84LGPRR18 pKa = 11.84LKK20 pKa = 10.82GNSPRR25 pKa = 11.84RR26 pKa = 11.84GPRR29 pKa = 11.84YY30 pKa = 9.24VVARR34 pKa = 11.84TAPVISAVTEE44 pKa = 4.09WKK46 pKa = 10.11RR47 pKa = 11.84RR48 pKa = 11.84PRR50 pKa = 11.84NRR52 pKa = 11.84NPKK55 pKa = 9.42SSKK58 pKa = 10.16KK59 pKa = 10.17DD60 pKa = 3.35VVPFGCEE67 pKa = 4.16GPCKK71 pKa = 10.47VEE73 pKa = 4.28SFSDD77 pKa = 3.52RR78 pKa = 11.84YY79 pKa = 8.86EE80 pKa = 4.11VKK82 pKa = 9.2HH83 pKa = 6.07TGTVCCLSQISRR95 pKa = 11.84GNGLKK100 pKa = 10.03QRR102 pKa = 11.84IGKK105 pKa = 9.95RR106 pKa = 11.84ITIKK110 pKa = 10.5SIYY113 pKa = 10.08LLGKK117 pKa = 9.56VWLDD121 pKa = 3.05GSNRR125 pKa = 11.84ISNHH129 pKa = 4.4TNVCIFFVVRR139 pKa = 11.84DD140 pKa = 3.97RR141 pKa = 11.84RR142 pKa = 11.84PSVTPINFGSLFDD155 pKa = 4.09MFDD158 pKa = 3.88GEE160 pKa = 4.39PATASVKK167 pKa = 10.18SDD169 pKa = 2.8HH170 pKa = 7.13RR171 pKa = 11.84DD172 pKa = 3.17RR173 pKa = 11.84FQVLYY178 pKa = 9.82RR179 pKa = 11.84FQVSVSGGQYY189 pKa = 9.94SSRR192 pKa = 11.84DD193 pKa = 3.07QTVFQKK199 pKa = 10.29FVNLNHH205 pKa = 6.56RR206 pKa = 11.84VVFDD210 pKa = 3.7NQDD213 pKa = 3.2DD214 pKa = 4.4GVYY217 pKa = 10.91GNHH220 pKa = 6.87DD221 pKa = 3.58QNALLVYY228 pKa = 7.32MACCHH233 pKa = 4.93STNWLYY239 pKa = 11.66SSMKK243 pKa = 10.04AKK245 pKa = 10.62VYY247 pKa = 10.2FYY249 pKa = 10.97DD250 pKa = 3.62SAMNN254 pKa = 3.79

Molecular weight: 28.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

992

107

362

198.4

22.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.435 ± 0.334

3.125 ± 0.763

5.746 ± 0.246

4.536 ± 1.155

4.839 ± 0.373

5.444 ± 0.635

4.133 ± 0.871

4.738 ± 0.497

5.645 ± 0.536

7.258 ± 1.276

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.512 ± 0.165

4.839 ± 0.782

6.048 ± 0.697

4.234 ± 0.528

7.359 ± 0.941

7.56 ± 0.712

6.754 ± 0.723

6.754 ± 1.32

1.21 ± 0.095

3.831 ± 0.256
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski