Amino acid dipepetide frequency for Streptococcus phage SW30

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.841AlaAla: 2.841 ± 0.908
0.098AlaCys: 0.098 ± 0.097
3.821AlaAsp: 3.821 ± 0.784
3.919AlaGlu: 3.919 ± 0.527
2.548AlaPhe: 2.548 ± 0.676
3.429AlaGly: 3.429 ± 0.738
0.686AlaHis: 0.686 ± 0.299
4.899AlaIle: 4.899 ± 0.94
6.565AlaLys: 6.565 ± 1.175
5.977AlaLeu: 5.977 ± 0.688
1.176AlaMet: 1.176 ± 0.347
4.997AlaAsn: 4.997 ± 0.849
1.372AlaPro: 1.372 ± 0.276
2.45AlaGln: 2.45 ± 0.502
2.156AlaArg: 2.156 ± 0.458
4.605AlaSer: 4.605 ± 0.795
4.605AlaThr: 4.605 ± 0.864
4.703AlaVal: 4.703 ± 0.642
0.98AlaTrp: 0.98 ± 0.232
2.156AlaTyr: 2.156 ± 0.45
0.0AlaXaa: 0.0 ± 0.0
Cys
0.49CysAla: 0.49 ± 0.198
0.098CysCys: 0.098 ± 0.1
0.882CysAsp: 0.882 ± 0.274
0.294CysGlu: 0.294 ± 0.147
0.392CysPhe: 0.392 ± 0.242
0.098CysGly: 0.098 ± 0.1
0.098CysHis: 0.098 ± 0.1
0.0CysIle: 0.0 ± 0.0
0.294CysLys: 0.294 ± 0.162
0.392CysLeu: 0.392 ± 0.224
0.098CysMet: 0.098 ± 0.097
0.294CysAsn: 0.294 ± 0.157
0.196CysPro: 0.196 ± 0.141
0.098CysGln: 0.098 ± 0.104
0.49CysArg: 0.49 ± 0.292
0.588CysSer: 0.588 ± 0.33
0.294CysThr: 0.294 ± 0.196
0.294CysVal: 0.294 ± 0.121
0.196CysTrp: 0.196 ± 0.151
0.098CysTyr: 0.098 ± 0.085
0.0CysXaa: 0.0 ± 0.0
Asp
3.625AspAla: 3.625 ± 0.629
0.49AspCys: 0.49 ± 0.208
4.997AspAsp: 4.997 ± 0.67
4.115AspGlu: 4.115 ± 0.596
3.331AspPhe: 3.331 ± 0.349
5.683AspGly: 5.683 ± 0.808
0.98AspHis: 0.98 ± 0.338
5.683AspIle: 5.683 ± 0.82
6.173AspLys: 6.173 ± 0.723
4.017AspLeu: 4.017 ± 0.616
2.156AspMet: 2.156 ± 0.496
4.115AspAsn: 4.115 ± 0.955
2.352AspPro: 2.352 ± 0.427
1.568AspGln: 1.568 ± 0.318
2.841AspArg: 2.841 ± 0.485
2.743AspSer: 2.743 ± 0.477
4.507AspThr: 4.507 ± 0.6
3.625AspVal: 3.625 ± 0.469
0.784AspTrp: 0.784 ± 0.28
2.841AspTyr: 2.841 ± 0.397
0.0AspXaa: 0.0 ± 0.0
Glu
3.625GluAla: 3.625 ± 0.479
0.294GluCys: 0.294 ± 0.143
3.625GluAsp: 3.625 ± 0.767
5.095GluGlu: 5.095 ± 0.966
2.254GluPhe: 2.254 ± 0.566
2.841GluGly: 2.841 ± 0.566
1.078GluHis: 1.078 ± 0.36
6.173GluIle: 6.173 ± 0.759
4.213GluLys: 4.213 ± 0.875
6.271GluLeu: 6.271 ± 0.722
2.156GluMet: 2.156 ± 0.518
4.115GluAsn: 4.115 ± 0.669
1.47GluPro: 1.47 ± 0.401
2.646GluGln: 2.646 ± 0.52
3.233GluArg: 3.233 ± 0.675
3.331GluSer: 3.331 ± 0.435
3.625GluThr: 3.625 ± 0.578
3.527GluVal: 3.527 ± 0.545
1.568GluTrp: 1.568 ± 0.298
3.429GluTyr: 3.429 ± 0.556
0.0GluXaa: 0.0 ± 0.0
Phe
2.841PheAla: 2.841 ± 0.537
0.392PheCys: 0.392 ± 0.236
4.115PheAsp: 4.115 ± 0.625
1.96PheGlu: 1.96 ± 0.489
1.96PhePhe: 1.96 ± 0.437
3.233PheGly: 3.233 ± 0.74
0.49PheHis: 0.49 ± 0.173
2.646PheIle: 2.646 ± 0.542
3.723PheLys: 3.723 ± 0.606
2.939PheLeu: 2.939 ± 0.487
0.686PheMet: 0.686 ± 0.273
2.939PheAsn: 2.939 ± 0.689
0.98PhePro: 0.98 ± 0.254
0.98PheGln: 0.98 ± 0.265
1.568PheArg: 1.568 ± 0.344
2.939PheSer: 2.939 ± 0.532
2.352PheThr: 2.352 ± 0.4
2.646PheVal: 2.646 ± 0.406
0.686PheTrp: 0.686 ± 0.201
2.058PheTyr: 2.058 ± 0.473
0.0PheXaa: 0.0 ± 0.0
Gly
2.841GlyAla: 2.841 ± 0.551
0.392GlyCys: 0.392 ± 0.203
3.919GlyAsp: 3.919 ± 0.549
3.723GlyGlu: 3.723 ± 0.727
4.115GlyPhe: 4.115 ± 0.844
4.409GlyGly: 4.409 ± 0.849
0.784GlyHis: 0.784 ± 0.308
4.213GlyIle: 4.213 ± 0.731
6.369GlyLys: 6.369 ± 0.85
6.271GlyLeu: 6.271 ± 0.882
1.274GlyMet: 1.274 ± 0.353
4.409GlyAsn: 4.409 ± 0.713
0.882GlyPro: 0.882 ± 0.329
3.135GlyGln: 3.135 ± 0.631
2.939GlyArg: 2.939 ± 0.444
4.409GlySer: 4.409 ± 0.748
4.213GlyThr: 4.213 ± 0.793
4.213GlyVal: 4.213 ± 0.669
1.176GlyTrp: 1.176 ± 0.376
3.037GlyTyr: 3.037 ± 0.549
0.0GlyXaa: 0.0 ± 0.0
His
0.196HisAla: 0.196 ± 0.122
0.098HisCys: 0.098 ± 0.096
1.078HisAsp: 1.078 ± 0.294
0.588HisGlu: 0.588 ± 0.267
0.686HisPhe: 0.686 ± 0.265
0.686HisGly: 0.686 ± 0.24
0.588HisHis: 0.588 ± 0.255
1.078HisIle: 1.078 ± 0.321
0.98HisLys: 0.98 ± 0.368
1.372HisLeu: 1.372 ± 0.289
0.588HisMet: 0.588 ± 0.237
0.686HisAsn: 0.686 ± 0.233
0.686HisPro: 0.686 ± 0.229
0.98HisGln: 0.98 ± 0.358
0.784HisArg: 0.784 ± 0.249
0.882HisSer: 0.882 ± 0.257
0.882HisThr: 0.882 ± 0.263
1.274HisVal: 1.274 ± 0.401
0.098HisTrp: 0.098 ± 0.093
0.686HisTyr: 0.686 ± 0.255
0.0HisXaa: 0.0 ± 0.0
Ile
4.703IleAla: 4.703 ± 0.921
0.49IleCys: 0.49 ± 0.234
5.487IleAsp: 5.487 ± 0.742
4.213IleGlu: 4.213 ± 0.732
1.274IlePhe: 1.274 ± 0.383
4.997IleGly: 4.997 ± 0.633
0.882IleHis: 0.882 ± 0.248
2.841IleIle: 2.841 ± 0.437
7.545IleLys: 7.545 ± 0.757
4.605IleLeu: 4.605 ± 0.853
1.764IleMet: 1.764 ± 0.509
3.527IleAsn: 3.527 ± 0.454
3.037IlePro: 3.037 ± 0.539
3.135IleGln: 3.135 ± 0.486
2.939IleArg: 2.939 ± 0.479
4.605IleSer: 4.605 ± 0.592
4.409IleThr: 4.409 ± 0.709
3.527IleVal: 3.527 ± 0.62
0.784IleTrp: 0.784 ± 0.214
2.254IleTyr: 2.254 ± 0.508
0.0IleXaa: 0.0 ± 0.0
Lys
5.781LysAla: 5.781 ± 0.681
0.392LysCys: 0.392 ± 0.216
5.193LysAsp: 5.193 ± 0.959
6.173LysGlu: 6.173 ± 0.919
2.841LysPhe: 2.841 ± 0.779
6.467LysGly: 6.467 ± 0.649
1.764LysHis: 1.764 ± 0.465
6.369LysIle: 6.369 ± 0.78
5.879LysLys: 5.879 ± 1.105
6.663LysLeu: 6.663 ± 0.837
2.254LysMet: 2.254 ± 0.511
4.605LysAsn: 4.605 ± 0.663
3.037LysPro: 3.037 ± 0.414
3.919LysGln: 3.919 ± 0.492
4.115LysArg: 4.115 ± 0.529
4.409LysSer: 4.409 ± 0.669
5.291LysThr: 5.291 ± 0.673
4.017LysVal: 4.017 ± 0.69
1.078LysTrp: 1.078 ± 0.283
4.115LysTyr: 4.115 ± 1.055
0.0LysXaa: 0.0 ± 0.0
Leu
6.565LeuAla: 6.565 ± 0.867
0.588LeuCys: 0.588 ± 0.256
5.487LeuAsp: 5.487 ± 0.764
6.075LeuGlu: 6.075 ± 0.896
2.939LeuPhe: 2.939 ± 0.421
5.879LeuGly: 5.879 ± 0.985
0.882LeuHis: 0.882 ± 0.275
3.527LeuIle: 3.527 ± 0.647
7.447LeuLys: 7.447 ± 0.83
4.801LeuLeu: 4.801 ± 0.671
2.156LeuMet: 2.156 ± 0.494
5.291LeuAsn: 5.291 ± 0.739
3.037LeuPro: 3.037 ± 0.397
3.037LeuGln: 3.037 ± 0.453
3.037LeuArg: 3.037 ± 0.667
4.899LeuSer: 4.899 ± 0.663
6.173LeuThr: 6.173 ± 0.858
3.527LeuVal: 3.527 ± 0.566
0.882LeuTrp: 0.882 ± 0.241
1.764LeuTyr: 1.764 ± 0.481
0.0LeuXaa: 0.0 ± 0.0
Met
2.058MetAla: 2.058 ± 0.423
0.0MetCys: 0.0 ± 0.0
0.588MetAsp: 0.588 ± 0.219
1.568MetGlu: 1.568 ± 0.473
1.176MetPhe: 1.176 ± 0.288
0.98MetGly: 0.98 ± 0.309
0.294MetHis: 0.294 ± 0.169
2.058MetIle: 2.058 ± 0.483
2.352MetLys: 2.352 ± 0.424
1.568MetLeu: 1.568 ± 0.347
0.49MetMet: 0.49 ± 0.182
1.176MetAsn: 1.176 ± 0.281
0.98MetPro: 0.98 ± 0.285
0.882MetGln: 0.882 ± 0.265
0.784MetArg: 0.784 ± 0.227
2.254MetSer: 2.254 ± 0.418
1.372MetThr: 1.372 ± 0.301
1.96MetVal: 1.96 ± 0.43
0.196MetTrp: 0.196 ± 0.107
0.784MetTyr: 0.784 ± 0.289
0.0MetXaa: 0.0 ± 0.0
Asn
5.193AsnAla: 5.193 ± 1.117
0.196AsnCys: 0.196 ± 0.14
3.625AsnAsp: 3.625 ± 0.492
3.821AsnGlu: 3.821 ± 0.717
2.841AsnPhe: 2.841 ± 0.536
7.447AsnGly: 7.447 ± 1.257
0.882AsnHis: 0.882 ± 0.254
3.625AsnIle: 3.625 ± 0.619
4.507AsnLys: 4.507 ± 0.517
4.409AsnLeu: 4.409 ± 0.534
1.47AsnMet: 1.47 ± 0.328
4.409AsnAsn: 4.409 ± 0.888
3.331AsnPro: 3.331 ± 0.586
2.841AsnGln: 2.841 ± 0.528
2.548AsnArg: 2.548 ± 0.432
4.311AsnSer: 4.311 ± 0.796
3.821AsnThr: 3.821 ± 0.465
3.527AsnVal: 3.527 ± 0.462
1.176AsnTrp: 1.176 ± 0.316
1.764AsnTyr: 1.764 ± 0.376
0.0AsnXaa: 0.0 ± 0.0
Pro
1.568ProAla: 1.568 ± 0.32
0.392ProCys: 0.392 ± 0.277
1.47ProAsp: 1.47 ± 0.397
2.156ProGlu: 2.156 ± 0.446
1.176ProPhe: 1.176 ± 0.35
0.882ProGly: 0.882 ± 0.299
0.49ProHis: 0.49 ± 0.176
2.156ProIle: 2.156 ± 0.355
3.527ProLys: 3.527 ± 0.544
2.548ProLeu: 2.548 ± 0.422
0.294ProMet: 0.294 ± 0.16
2.743ProAsn: 2.743 ± 0.402
0.49ProPro: 0.49 ± 0.265
0.882ProGln: 0.882 ± 0.268
1.176ProArg: 1.176 ± 0.486
2.841ProSer: 2.841 ± 0.587
1.96ProThr: 1.96 ± 0.342
1.568ProVal: 1.568 ± 0.37
0.392ProTrp: 0.392 ± 0.137
1.372ProTyr: 1.372 ± 0.446
0.0ProXaa: 0.0 ± 0.0
Gln
4.017GlnAla: 4.017 ± 0.628
0.098GlnCys: 0.098 ± 0.093
1.96GlnAsp: 1.96 ± 0.325
2.254GlnGlu: 2.254 ± 0.452
1.176GlnPhe: 1.176 ± 0.247
3.037GlnGly: 3.037 ± 0.603
0.784GlnHis: 0.784 ± 0.338
2.45GlnIle: 2.45 ± 0.599
3.135GlnLys: 3.135 ± 0.548
3.331GlnLeu: 3.331 ± 0.483
1.372GlnMet: 1.372 ± 0.366
2.45GlnAsn: 2.45 ± 0.496
0.294GlnPro: 0.294 ± 0.144
2.743GlnGln: 2.743 ± 0.515
2.058GlnArg: 2.058 ± 0.401
2.254GlnSer: 2.254 ± 0.446
3.331GlnThr: 3.331 ± 0.483
2.548GlnVal: 2.548 ± 0.59
0.392GlnTrp: 0.392 ± 0.206
1.764GlnTyr: 1.764 ± 0.353
0.0GlnXaa: 0.0 ± 0.0
Arg
2.156ArgAla: 2.156 ± 0.38
0.196ArgCys: 0.196 ± 0.145
2.45ArgAsp: 2.45 ± 0.47
2.646ArgGlu: 2.646 ± 0.686
2.352ArgPhe: 2.352 ± 0.474
2.548ArgGly: 2.548 ± 0.546
0.686ArgHis: 0.686 ± 0.245
3.037ArgIle: 3.037 ± 0.553
3.527ArgLys: 3.527 ± 0.787
3.135ArgLeu: 3.135 ± 0.581
0.784ArgMet: 0.784 ± 0.252
3.233ArgAsn: 3.233 ± 0.388
1.176ArgPro: 1.176 ± 0.276
1.96ArgGln: 1.96 ± 0.478
1.47ArgArg: 1.47 ± 0.298
1.666ArgSer: 1.666 ± 0.346
3.037ArgThr: 3.037 ± 0.8
2.548ArgVal: 2.548 ± 0.476
1.274ArgTrp: 1.274 ± 0.304
2.45ArgTyr: 2.45 ± 0.536
0.0ArgXaa: 0.0 ± 0.0
Ser
3.821SerAla: 3.821 ± 0.517
0.392SerCys: 0.392 ± 0.21
4.311SerAsp: 4.311 ± 0.482
3.723SerGlu: 3.723 ± 0.55
2.841SerPhe: 2.841 ± 0.416
4.213SerGly: 4.213 ± 0.636
0.49SerHis: 0.49 ± 0.185
4.605SerIle: 4.605 ± 0.614
4.997SerLys: 4.997 ± 0.948
4.017SerLeu: 4.017 ± 0.645
1.666SerMet: 1.666 ± 0.4
5.291SerAsn: 5.291 ± 0.679
1.666SerPro: 1.666 ± 0.363
2.841SerGln: 2.841 ± 0.533
3.037SerArg: 3.037 ± 0.562
3.331SerSer: 3.331 ± 0.528
4.017SerThr: 4.017 ± 0.594
5.193SerVal: 5.193 ± 0.827
0.686SerTrp: 0.686 ± 0.245
1.764SerTyr: 1.764 ± 0.492
0.0SerXaa: 0.0 ± 0.0
Thr
4.409ThrAla: 4.409 ± 0.721
0.294ThrCys: 0.294 ± 0.162
4.801ThrAsp: 4.801 ± 0.612
3.919ThrGlu: 3.919 ± 0.608
3.037ThrPhe: 3.037 ± 0.576
3.429ThrGly: 3.429 ± 0.517
1.176ThrHis: 1.176 ± 0.358
4.213ThrIle: 4.213 ± 0.638
5.193ThrLys: 5.193 ± 0.781
7.251ThrLeu: 7.251 ± 0.783
1.078ThrMet: 1.078 ± 0.28
4.311ThrAsn: 4.311 ± 0.596
1.96ThrPro: 1.96 ± 0.466
2.352ThrGln: 2.352 ± 0.584
1.96ThrArg: 1.96 ± 0.426
3.919ThrSer: 3.919 ± 0.521
4.017ThrThr: 4.017 ± 0.752
3.331ThrVal: 3.331 ± 0.541
0.784ThrTrp: 0.784 ± 0.285
3.429ThrTyr: 3.429 ± 0.723
0.0ThrXaa: 0.0 ± 0.0
Val
3.919ValAla: 3.919 ± 0.635
0.294ValCys: 0.294 ± 0.14
4.997ValAsp: 4.997 ± 0.537
4.213ValGlu: 4.213 ± 0.65
2.548ValPhe: 2.548 ± 0.686
3.919ValGly: 3.919 ± 0.603
0.49ValHis: 0.49 ± 0.173
4.703ValIle: 4.703 ± 0.7
4.311ValLys: 4.311 ± 0.632
3.625ValLeu: 3.625 ± 0.674
1.078ValMet: 1.078 ± 0.297
4.213ValAsn: 4.213 ± 0.817
1.372ValPro: 1.372 ± 0.322
1.862ValGln: 1.862 ± 0.426
2.058ValArg: 2.058 ± 0.624
5.291ValSer: 5.291 ± 0.687
4.703ValThr: 4.703 ± 1.011
4.017ValVal: 4.017 ± 0.814
0.98ValTrp: 0.98 ± 0.25
1.764ValTyr: 1.764 ± 0.427
0.0ValXaa: 0.0 ± 0.0
Trp
0.686TrpAla: 0.686 ± 0.215
0.0TrpCys: 0.0 ± 0.0
1.078TrpAsp: 1.078 ± 0.29
1.372TrpGlu: 1.372 ± 0.343
0.588TrpPhe: 0.588 ± 0.228
0.784TrpGly: 0.784 ± 0.262
0.392TrpHis: 0.392 ± 0.221
0.49TrpIle: 0.49 ± 0.152
0.784TrpLys: 0.784 ± 0.325
0.98TrpLeu: 0.98 ± 0.319
0.098TrpMet: 0.098 ± 0.092
0.98TrpAsn: 0.98 ± 0.344
0.196TrpPro: 0.196 ± 0.146
0.784TrpGln: 0.784 ± 0.234
0.686TrpArg: 0.686 ± 0.232
1.568TrpSer: 1.568 ± 0.599
0.686TrpThr: 0.686 ± 0.193
1.568TrpVal: 1.568 ± 0.316
0.294TrpTrp: 0.294 ± 0.211
0.588TrpTyr: 0.588 ± 0.248
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.646TyrAla: 2.646 ± 0.385
0.49TyrCys: 0.49 ± 0.261
2.841TyrAsp: 2.841 ± 0.442
2.743TyrGlu: 2.743 ± 0.569
2.058TyrPhe: 2.058 ± 0.393
1.764TyrGly: 1.764 ± 0.425
0.98TyrHis: 0.98 ± 0.301
2.254TyrIle: 2.254 ± 0.386
2.841TyrLys: 2.841 ± 0.524
3.821TyrLeu: 3.821 ± 0.53
0.784TyrMet: 0.784 ± 0.307
1.96TyrAsn: 1.96 ± 0.409
1.568TyrPro: 1.568 ± 0.389
2.352TyrGln: 2.352 ± 0.395
2.45TyrArg: 2.45 ± 0.385
2.058TyrSer: 2.058 ± 0.591
1.568TyrThr: 1.568 ± 0.359
2.743TyrVal: 2.743 ± 0.578
0.196TyrTrp: 0.196 ± 0.129
2.058TyrTyr: 2.058 ± 0.64
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 41 proteins (10207 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski