Amino acid dipepetide frequency for Gordonia phage Octobien14

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.597AlaAla: 12.597 ± 1.348
0.963AlaCys: 0.963 ± 0.226
7.366AlaAsp: 7.366 ± 0.567
6.989AlaGlu: 6.989 ± 0.656
3.181AlaPhe: 3.181 ± 0.378
8.328AlaGly: 8.328 ± 0.726
1.967AlaHis: 1.967 ± 0.289
4.018AlaIle: 4.018 ± 0.508
6.947AlaLys: 6.947 ± 0.544
10.462AlaLeu: 10.462 ± 0.694
3.432AlaMet: 3.432 ± 0.379
4.185AlaAsn: 4.185 ± 0.455
4.394AlaPro: 4.394 ± 0.528
3.85AlaGln: 3.85 ± 0.384
6.152AlaArg: 6.152 ± 0.556
5.106AlaSer: 5.106 ± 0.476
6.068AlaThr: 6.068 ± 0.631
7.073AlaVal: 7.073 ± 0.54
1.967AlaTrp: 1.967 ± 0.296
2.929AlaTyr: 2.929 ± 0.38
0.0AlaXaa: 0.0 ± 0.0
Cys
1.172CysAla: 1.172 ± 0.262
0.377CysCys: 0.377 ± 0.179
0.879CysAsp: 0.879 ± 0.215
0.46CysGlu: 0.46 ± 0.174
0.251CysPhe: 0.251 ± 0.099
1.172CysGly: 1.172 ± 0.247
0.251CysHis: 0.251 ± 0.11
0.46CysIle: 0.46 ± 0.121
0.293CysLys: 0.293 ± 0.105
0.46CysLeu: 0.46 ± 0.14
0.167CysMet: 0.167 ± 0.081
0.418CysAsn: 0.418 ± 0.141
0.418CysPro: 0.418 ± 0.151
0.335CysGln: 0.335 ± 0.128
0.879CysArg: 0.879 ± 0.216
0.544CysSer: 0.544 ± 0.138
0.544CysThr: 0.544 ± 0.172
0.628CysVal: 0.628 ± 0.189
0.335CysTrp: 0.335 ± 0.143
0.377CysTyr: 0.377 ± 0.137
0.0CysXaa: 0.0 ± 0.0
Asp
6.654AspAla: 6.654 ± 0.51
0.544AspCys: 0.544 ± 0.161
5.315AspAsp: 5.315 ± 0.571
5.357AspGlu: 5.357 ± 0.452
2.092AspPhe: 2.092 ± 0.287
5.44AspGly: 5.44 ± 0.442
1.339AspHis: 1.339 ± 0.254
2.344AspIle: 2.344 ± 0.265
2.302AspLys: 2.302 ± 0.372
5.189AspLeu: 5.189 ± 0.515
1.339AspMet: 1.339 ± 0.23
1.967AspAsn: 1.967 ± 0.287
4.813AspPro: 4.813 ± 0.382
2.678AspGln: 2.678 ± 0.321
3.39AspArg: 3.39 ± 0.442
3.557AspSer: 3.557 ± 0.337
3.222AspThr: 3.222 ± 0.418
4.269AspVal: 4.269 ± 0.408
1.8AspTrp: 1.8 ± 0.277
2.511AspTyr: 2.511 ± 0.392
0.0AspXaa: 0.0 ± 0.0
Glu
7.784GluAla: 7.784 ± 0.646
0.586GluCys: 0.586 ± 0.152
4.143GluAsp: 4.143 ± 0.475
3.85GluGlu: 3.85 ± 0.506
2.134GluPhe: 2.134 ± 0.251
3.641GluGly: 3.641 ± 0.417
1.632GluHis: 1.632 ± 0.284
2.595GluIle: 2.595 ± 0.282
2.092GluLys: 2.092 ± 0.313
7.366GluLeu: 7.366 ± 0.598
1.465GluMet: 1.465 ± 0.248
1.883GluAsn: 1.883 ± 0.329
3.222GluPro: 3.222 ± 0.47
2.385GluGln: 2.385 ± 0.348
3.892GluArg: 3.892 ± 0.396
3.892GluSer: 3.892 ± 0.438
2.804GluThr: 2.804 ± 0.324
5.106GluVal: 5.106 ± 0.495
1.548GluTrp: 1.548 ± 0.261
1.925GluTyr: 1.925 ± 0.335
0.0GluXaa: 0.0 ± 0.0
Phe
2.637PheAla: 2.637 ± 0.321
0.377PheCys: 0.377 ± 0.117
2.134PheAsp: 2.134 ± 0.251
2.344PheGlu: 2.344 ± 0.281
0.753PhePhe: 0.753 ± 0.183
3.055PheGly: 3.055 ± 0.369
0.963PheHis: 0.963 ± 0.215
1.214PheIle: 1.214 ± 0.216
1.423PheLys: 1.423 ± 0.296
2.302PheLeu: 2.302 ± 0.3
1.381PheMet: 1.381 ± 0.249
1.297PheAsn: 1.297 ± 0.261
1.381PhePro: 1.381 ± 0.222
0.963PheGln: 0.963 ± 0.189
1.841PheArg: 1.841 ± 0.244
1.883PheSer: 1.883 ± 0.328
2.344PheThr: 2.344 ± 0.379
2.302PheVal: 2.302 ± 0.285
0.753PheTrp: 0.753 ± 0.196
0.879PheTyr: 0.879 ± 0.178
0.0PheXaa: 0.0 ± 0.0
Gly
7.533GlyAla: 7.533 ± 0.826
0.753GlyCys: 0.753 ± 0.247
5.357GlyAsp: 5.357 ± 0.557
4.771GlyGlu: 4.771 ± 0.506
3.181GlyPhe: 3.181 ± 0.383
7.533GlyGly: 7.533 ± 1.448
1.8GlyHis: 1.8 ± 0.291
3.766GlyIle: 3.766 ± 0.395
3.725GlyLys: 3.725 ± 0.349
6.236GlyLeu: 6.236 ± 0.532
2.26GlyMet: 2.26 ± 0.301
2.344GlyAsn: 2.344 ± 0.306
3.306GlyPro: 3.306 ± 0.443
2.762GlyGln: 2.762 ± 0.312
4.729GlyArg: 4.729 ± 0.497
5.733GlySer: 5.733 ± 0.567
5.022GlyThr: 5.022 ± 0.421
7.617GlyVal: 7.617 ± 0.652
1.297GlyTrp: 1.297 ± 0.236
3.055GlyTyr: 3.055 ± 0.399
0.0GlyXaa: 0.0 ± 0.0
His
1.381HisAla: 1.381 ± 0.203
0.126HisCys: 0.126 ± 0.067
0.837HisAsp: 0.837 ± 0.183
1.172HisGlu: 1.172 ± 0.213
0.544HisPhe: 0.544 ± 0.154
1.548HisGly: 1.548 ± 0.266
0.544HisHis: 0.544 ± 0.141
0.879HisIle: 0.879 ± 0.204
0.837HisLys: 0.837 ± 0.185
1.925HisLeu: 1.925 ± 0.331
0.67HisMet: 0.67 ± 0.149
0.544HisAsn: 0.544 ± 0.152
1.297HisPro: 1.297 ± 0.263
0.293HisGln: 0.293 ± 0.123
1.172HisArg: 1.172 ± 0.231
1.046HisSer: 1.046 ± 0.25
1.088HisThr: 1.088 ± 0.169
1.925HisVal: 1.925 ± 0.304
0.418HisTrp: 0.418 ± 0.139
1.172HisTyr: 1.172 ± 0.217
0.0HisXaa: 0.0 ± 0.0
Ile
5.608IleAla: 5.608 ± 0.457
0.418IleCys: 0.418 ± 0.144
2.72IleAsp: 2.72 ± 0.376
3.348IleGlu: 3.348 ± 0.359
0.837IlePhe: 0.837 ± 0.183
3.432IleGly: 3.432 ± 0.369
0.544IleHis: 0.544 ± 0.18
1.214IleIle: 1.214 ± 0.191
1.507IleLys: 1.507 ± 0.248
2.846IleLeu: 2.846 ± 0.427
1.004IleMet: 1.004 ± 0.208
1.297IleAsn: 1.297 ± 0.246
2.218IlePro: 2.218 ± 0.269
1.507IleGln: 1.507 ± 0.245
3.139IleArg: 3.139 ± 0.323
1.674IleSer: 1.674 ± 0.266
3.515IleThr: 3.515 ± 0.337
2.929IleVal: 2.929 ± 0.359
0.795IleTrp: 0.795 ± 0.228
1.214IleTyr: 1.214 ± 0.186
0.0IleXaa: 0.0 ± 0.0
Lys
6.068LysAla: 6.068 ± 0.654
0.544LysCys: 0.544 ± 0.153
2.218LysAsp: 2.218 ± 0.34
2.511LysGlu: 2.511 ± 0.37
1.423LysPhe: 1.423 ± 0.225
4.352LysGly: 4.352 ± 0.463
1.465LysHis: 1.465 ± 0.215
1.967LysIle: 1.967 ± 0.327
2.511LysLys: 2.511 ± 0.381
3.725LysLeu: 3.725 ± 0.345
1.255LysMet: 1.255 ± 0.238
1.214LysAsn: 1.214 ± 0.259
3.348LysPro: 3.348 ± 0.529
1.841LysGln: 1.841 ± 0.288
2.804LysArg: 2.804 ± 0.289
3.181LysSer: 3.181 ± 0.448
1.8LysThr: 1.8 ± 0.308
3.264LysVal: 3.264 ± 0.354
0.753LysTrp: 0.753 ± 0.185
0.963LysTyr: 0.963 ± 0.209
0.0LysXaa: 0.0 ± 0.0
Leu
10.128LeuAla: 10.128 ± 0.752
0.963LeuCys: 0.963 ± 0.231
5.692LeuAsp: 5.692 ± 0.517
4.645LeuGlu: 4.645 ± 0.418
2.092LeuPhe: 2.092 ± 0.27
6.822LeuGly: 6.822 ± 0.542
1.59LeuHis: 1.59 ± 0.254
3.348LeuIle: 3.348 ± 0.404
4.394LeuLys: 4.394 ± 0.454
7.742LeuLeu: 7.742 ± 0.566
1.674LeuMet: 1.674 ± 0.268
2.218LeuAsn: 2.218 ± 0.262
4.018LeuPro: 4.018 ± 0.454
2.009LeuGln: 2.009 ± 0.298
5.608LeuArg: 5.608 ± 0.464
5.273LeuSer: 5.273 ± 0.459
5.064LeuThr: 5.064 ± 0.404
6.696LeuVal: 6.696 ± 0.534
1.674LeuTrp: 1.674 ± 0.266
2.218LeuTyr: 2.218 ± 0.353
0.0LeuXaa: 0.0 ± 0.0
Met
2.344MetAla: 2.344 ± 0.304
0.251MetCys: 0.251 ± 0.152
1.214MetAsp: 1.214 ± 0.188
1.046MetGlu: 1.046 ± 0.2
1.088MetPhe: 1.088 ± 0.178
1.841MetGly: 1.841 ± 0.263
0.377MetHis: 0.377 ± 0.125
0.879MetIle: 0.879 ± 0.169
1.046MetLys: 1.046 ± 0.228
1.925MetLeu: 1.925 ± 0.289
0.377MetMet: 0.377 ± 0.133
0.67MetAsn: 0.67 ± 0.164
1.507MetPro: 1.507 ± 0.262
0.837MetGln: 0.837 ± 0.161
2.092MetArg: 2.092 ± 0.282
2.804MetSer: 2.804 ± 0.301
1.967MetThr: 1.967 ± 0.279
0.963MetVal: 0.963 ± 0.165
0.418MetTrp: 0.418 ± 0.126
0.46MetTyr: 0.46 ± 0.191
0.0MetXaa: 0.0 ± 0.0
Asn
3.222AsnAla: 3.222 ± 0.414
0.209AsnCys: 0.209 ± 0.093
1.841AsnAsp: 1.841 ± 0.291
2.344AsnGlu: 2.344 ± 0.292
0.795AsnPhe: 0.795 ± 0.217
3.683AsnGly: 3.683 ± 0.298
0.544AsnHis: 0.544 ± 0.118
1.507AsnIle: 1.507 ± 0.217
1.172AsnLys: 1.172 ± 0.206
3.013AsnLeu: 3.013 ± 0.286
0.502AsnMet: 0.502 ± 0.131
1.214AsnAsn: 1.214 ± 0.24
2.469AsnPro: 2.469 ± 0.334
0.879AsnGln: 0.879 ± 0.204
2.511AsnArg: 2.511 ± 0.323
1.632AsnSer: 1.632 ± 0.298
2.051AsnThr: 2.051 ± 0.269
1.674AsnVal: 1.674 ± 0.255
0.46AsnTrp: 0.46 ± 0.157
0.963AsnTyr: 0.963 ± 0.205
0.0AsnXaa: 0.0 ± 0.0
Pro
6.194ProAla: 6.194 ± 0.506
0.46ProCys: 0.46 ± 0.145
4.436ProAsp: 4.436 ± 0.509
3.264ProGlu: 3.264 ± 0.424
1.8ProPhe: 1.8 ± 0.256
3.976ProGly: 3.976 ± 0.415
0.544ProHis: 0.544 ± 0.147
1.8ProIle: 1.8 ± 0.273
3.306ProLys: 3.306 ± 0.477
3.39ProLeu: 3.39 ± 0.366
1.172ProMet: 1.172 ± 0.223
1.841ProAsn: 1.841 ± 0.235
1.883ProPro: 1.883 ± 0.27
1.172ProGln: 1.172 ± 0.201
2.176ProArg: 2.176 ± 0.372
2.427ProSer: 2.427 ± 0.313
3.222ProThr: 3.222 ± 0.439
4.436ProVal: 4.436 ± 0.449
1.507ProTrp: 1.507 ± 0.3
1.339ProTyr: 1.339 ± 0.243
0.0ProXaa: 0.0 ± 0.0
Gln
4.143GlnAla: 4.143 ± 0.458
0.167GlnCys: 0.167 ± 0.076
1.841GlnAsp: 1.841 ± 0.286
2.678GlnGlu: 2.678 ± 0.393
1.632GlnPhe: 1.632 ± 0.238
2.344GlnGly: 2.344 ± 0.405
0.795GlnHis: 0.795 ± 0.177
1.548GlnIle: 1.548 ± 0.262
1.423GlnLys: 1.423 ± 0.191
2.971GlnLeu: 2.971 ± 0.305
1.172GlnMet: 1.172 ± 0.232
1.088GlnAsn: 1.088 ± 0.229
1.172GlnPro: 1.172 ± 0.228
1.214GlnGln: 1.214 ± 0.217
1.59GlnArg: 1.59 ± 0.208
1.758GlnSer: 1.758 ± 0.202
1.214GlnThr: 1.214 ± 0.276
2.511GlnVal: 2.511 ± 0.315
1.088GlnTrp: 1.088 ± 0.178
1.297GlnTyr: 1.297 ± 0.233
0.0GlnXaa: 0.0 ± 0.0
Arg
5.817ArgAla: 5.817 ± 0.53
0.586ArgCys: 0.586 ± 0.161
3.599ArgAsp: 3.599 ± 0.4
4.687ArgGlu: 4.687 ± 0.471
2.176ArgPhe: 2.176 ± 0.322
3.976ArgGly: 3.976 ± 0.454
1.13ArgHis: 1.13 ± 0.248
3.306ArgIle: 3.306 ± 0.406
3.976ArgLys: 3.976 ± 0.482
4.771ArgLeu: 4.771 ± 0.446
1.632ArgMet: 1.632 ± 0.237
1.883ArgAsn: 1.883 ± 0.228
2.469ArgPro: 2.469 ± 0.388
2.092ArgGln: 2.092 ± 0.354
3.766ArgArg: 3.766 ± 0.432
2.888ArgSer: 2.888 ± 0.389
3.892ArgThr: 3.892 ± 0.43
4.896ArgVal: 4.896 ± 0.475
1.172ArgTrp: 1.172 ± 0.207
1.59ArgTyr: 1.59 ± 0.241
0.0ArgXaa: 0.0 ± 0.0
Ser
5.692SerAla: 5.692 ± 0.504
0.544SerCys: 0.544 ± 0.158
3.474SerAsp: 3.474 ± 0.361
3.222SerGlu: 3.222 ± 0.399
2.302SerPhe: 2.302 ± 0.39
4.603SerGly: 4.603 ± 0.503
0.837SerHis: 0.837 ± 0.228
3.181SerIle: 3.181 ± 0.348
2.762SerLys: 2.762 ± 0.402
5.357SerLeu: 5.357 ± 0.479
0.963SerMet: 0.963 ± 0.203
1.758SerAsn: 1.758 ± 0.248
3.432SerPro: 3.432 ± 0.458
1.758SerGln: 1.758 ± 0.294
3.432SerArg: 3.432 ± 0.349
4.311SerSer: 4.311 ± 0.709
3.474SerThr: 3.474 ± 0.42
4.436SerVal: 4.436 ± 0.517
1.507SerTrp: 1.507 ± 0.272
1.632SerTyr: 1.632 ± 0.334
0.0SerXaa: 0.0 ± 0.0
Thr
5.608ThrAla: 5.608 ± 0.433
0.544ThrCys: 0.544 ± 0.183
3.599ThrAsp: 3.599 ± 0.438
3.348ThrGlu: 3.348 ± 0.344
2.009ThrPhe: 2.009 ± 0.275
5.901ThrGly: 5.901 ± 0.542
0.753ThrHis: 0.753 ± 0.197
3.055ThrIle: 3.055 ± 0.421
2.218ThrLys: 2.218 ± 0.319
4.394ThrLeu: 4.394 ± 0.423
1.214ThrMet: 1.214 ± 0.266
1.883ThrAsn: 1.883 ± 0.252
3.85ThrPro: 3.85 ± 0.381
2.762ThrGln: 2.762 ± 0.295
3.264ThrArg: 3.264 ± 0.299
2.971ThrSer: 2.971 ± 0.39
3.641ThrThr: 3.641 ± 0.395
5.106ThrVal: 5.106 ± 0.491
0.753ThrTrp: 0.753 ± 0.164
1.716ThrTyr: 1.716 ± 0.249
0.0ThrXaa: 0.0 ± 0.0
Val
8.496ValAla: 8.496 ± 0.675
1.381ValCys: 1.381 ± 0.282
5.901ValAsp: 5.901 ± 0.513
4.645ValGlu: 4.645 ± 0.458
2.26ValPhe: 2.26 ± 0.259
6.319ValGly: 6.319 ± 0.483
1.507ValHis: 1.507 ± 0.192
3.348ValIle: 3.348 ± 0.358
3.641ValLys: 3.641 ± 0.396
5.566ValLeu: 5.566 ± 0.372
1.13ValMet: 1.13 ± 0.224
3.055ValAsn: 3.055 ± 0.31
3.474ValPro: 3.474 ± 0.477
2.009ValGln: 2.009 ± 0.252
4.311ValArg: 4.311 ± 0.471
4.352ValSer: 4.352 ± 0.597
4.771ValThr: 4.771 ± 0.53
5.943ValVal: 5.943 ± 0.583
1.339ValTrp: 1.339 ± 0.283
2.009ValTyr: 2.009 ± 0.308
0.0ValXaa: 0.0 ± 0.0
Trp
2.26TrpAla: 2.26 ± 0.324
0.251TrpCys: 0.251 ± 0.095
1.548TrpAsp: 1.548 ± 0.266
1.59TrpGlu: 1.59 ± 0.256
0.628TrpPhe: 0.628 ± 0.174
1.507TrpGly: 1.507 ± 0.319
0.251TrpHis: 0.251 ± 0.107
0.837TrpIle: 0.837 ± 0.169
0.586TrpLys: 0.586 ± 0.154
1.841TrpLeu: 1.841 ± 0.23
0.628TrpMet: 0.628 ± 0.178
0.879TrpAsn: 0.879 ± 0.216
0.753TrpPro: 0.753 ± 0.176
0.711TrpGln: 0.711 ± 0.16
1.297TrpArg: 1.297 ± 0.196
1.59TrpSer: 1.59 ± 0.282
1.046TrpThr: 1.046 ± 0.207
1.758TrpVal: 1.758 ± 0.271
0.544TrpTrp: 0.544 ± 0.153
0.377TrpTyr: 0.377 ± 0.11
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.637TyrAla: 2.637 ± 0.31
0.377TyrCys: 0.377 ± 0.158
2.009TyrAsp: 2.009 ± 0.293
1.674TyrGlu: 1.674 ± 0.282
1.046TyrPhe: 1.046 ± 0.179
3.306TyrGly: 3.306 ± 0.391
0.586TyrHis: 0.586 ± 0.187
0.502TyrIle: 0.502 ± 0.13
1.046TyrLys: 1.046 ± 0.184
2.385TyrLeu: 2.385 ± 0.318
0.628TyrMet: 0.628 ± 0.164
1.046TyrAsn: 1.046 ± 0.195
0.921TyrPro: 0.921 ± 0.169
1.59TyrGln: 1.59 ± 0.296
2.26TyrArg: 2.26 ± 0.297
2.051TyrSer: 2.051 ± 0.332
1.883TyrThr: 1.883 ± 0.278
1.925TyrVal: 1.925 ± 0.346
0.753TyrTrp: 0.753 ± 0.151
0.963TyrTyr: 0.963 ± 0.169
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 143 proteins (23896 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski