Diutina rugosa (Yeast) (Candida rugosa)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetales incertae sedis; Diutina

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5811 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A642V1E8|A0A642V1E8_DIURU J domain-containing protein OS=Diutina rugosa OX=5481 GN=DIURU_001284 PE=3 SV=1
MM1 pKa = 7.97IIRR4 pKa = 11.84SMLLPLVAALPVLVPRR20 pKa = 11.84DD21 pKa = 3.87ADD23 pKa = 4.07DD24 pKa = 5.44DD25 pKa = 5.0DD26 pKa = 4.87NGPVTAYY33 pKa = 8.84IQPVVAVAEE42 pKa = 4.4ATTTVQPAVVYY53 pKa = 10.77VDD55 pKa = 4.49ANGNTVGGVSGAVSIDD71 pKa = 3.27DD72 pKa = 4.01SQFGTSFYY80 pKa = 7.83TTSAPPLITGDD91 pKa = 4.11EE92 pKa = 4.07NDD94 pKa = 4.17LDD96 pKa = 4.16VGGGNQDD103 pKa = 3.25NNNNNGPAAAPTAQVTNDD121 pKa = 3.81SGNGDD126 pKa = 3.84SPNTQQNNNNNVQVVEE142 pKa = 4.37TVNFGPSDD150 pKa = 3.55IVTVAATTIYY160 pKa = 10.56GPQAQDD166 pKa = 3.03NAGQQQQTSTTEE178 pKa = 3.91PQPQPTTTEE187 pKa = 3.92QQQDD191 pKa = 3.43QPTTTEE197 pKa = 3.84QPTTEE202 pKa = 4.19QPTTEE207 pKa = 4.1QPTTEE212 pKa = 4.18QQPSTEE218 pKa = 4.73PSTQPAPQPSTTQADD233 pKa = 3.66NQGDD237 pKa = 3.88NNQGNNNNNQGNNNNNSGNDD257 pKa = 3.52SNSNQGNNDD266 pKa = 3.27NNNNSDD272 pKa = 3.91NNNNYY277 pKa = 10.28NNNQGNDD284 pKa = 3.41NNQGNDD290 pKa = 3.28NNQGNDD296 pKa = 3.28NNQGNDD302 pKa = 3.23NNNNNGGSSDD312 pKa = 3.85GLLTPPVYY320 pKa = 9.8IVYY323 pKa = 10.32SPYY326 pKa = 11.32NNDD329 pKa = 3.32QSCKK333 pKa = 10.08DD334 pKa = 3.44AATVRR339 pKa = 11.84SDD341 pKa = 4.0LAIVKK346 pKa = 9.97QKK348 pKa = 10.85GINKK352 pKa = 8.22VRR354 pKa = 11.84IYY356 pKa = 11.03GSDD359 pKa = 3.05CGAFTNILPACKK371 pKa = 10.17DD372 pKa = 3.11LGLKK376 pKa = 9.56VNQGFWFQPGQGPDD390 pKa = 3.61SIDD393 pKa = 3.4GGVSEE398 pKa = 5.52FISWGQSNGWDD409 pKa = 3.1IIDD412 pKa = 3.98FVTVGNEE419 pKa = 3.6AVNDD423 pKa = 4.17GNLSPDD429 pKa = 3.39QLNGKK434 pKa = 9.13LSSVRR439 pKa = 11.84GQLQAAGYY447 pKa = 8.45SGSVITSEE455 pKa = 4.26PPISFIRR462 pKa = 11.84HH463 pKa = 6.22PEE465 pKa = 3.71LCEE468 pKa = 3.52QSDD471 pKa = 4.17FIGTNIHH478 pKa = 6.66SYY480 pKa = 10.79FNTGVAPDD488 pKa = 3.79GAGEE492 pKa = 4.01YY493 pKa = 10.53LKK495 pKa = 10.38QQNEE499 pKa = 4.37AVAQACPNKK508 pKa = 9.96KK509 pKa = 9.81IYY511 pKa = 8.77VTEE514 pKa = 3.42TGYY517 pKa = 9.57PHH519 pKa = 7.46KK520 pKa = 10.83GATNGLNVPSKK531 pKa = 9.8EE532 pKa = 3.82NQKK535 pKa = 10.0IVFEE539 pKa = 5.07SIWNTIGNDD548 pKa = 3.46VTVLAMWDD556 pKa = 4.46DD557 pKa = 3.84YY558 pKa = 10.85WKK560 pKa = 11.19NPGPYY565 pKa = 10.11GIEE568 pKa = 4.0QYY570 pKa = 10.78FGALDD575 pKa = 3.85LFSS578 pKa = 4.27

Molecular weight: 61.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A642URN3|A0A642URN3_DIURU Uncharacterized protein OS=Diutina rugosa OX=5481 GN=DIURU_001983 PE=3 SV=1
MM1 pKa = 7.13VFSKK5 pKa = 10.12FKK7 pKa = 11.17ANIVDD12 pKa = 3.56EE13 pKa = 4.79HH14 pKa = 6.81NGSGLISKK22 pKa = 7.86RR23 pKa = 11.84TRR25 pKa = 11.84VVSARR30 pKa = 11.84GGASRR35 pKa = 11.84TASPEE40 pKa = 3.49APRR43 pKa = 11.84YY44 pKa = 9.04LDD46 pKa = 3.34HH47 pKa = 7.28HH48 pKa = 6.92AAPPAAVRR56 pKa = 11.84TQSQQLYY63 pKa = 8.28SQPHH67 pKa = 5.49HH68 pKa = 6.3HH69 pKa = 7.15HH70 pKa = 7.29PPGSWIDD77 pKa = 3.6PRR79 pKa = 11.84ASAQRR84 pKa = 11.84STPPRR89 pKa = 11.84ASAQRR94 pKa = 11.84STPPSSSAASSVFSNPAPARR114 pKa = 11.84RR115 pKa = 11.84PSVTYY120 pKa = 9.76RR121 pKa = 11.84HH122 pKa = 6.16SAPSVKK128 pKa = 10.02VASSEE133 pKa = 4.11SSSPVSEE140 pKa = 4.84HH141 pKa = 5.38SAEE144 pKa = 4.71AIFDD148 pKa = 3.72SAPPRR153 pKa = 11.84KK154 pKa = 9.72SLLKK158 pKa = 10.37RR159 pKa = 11.84LNSQKK164 pKa = 9.29QRR166 pKa = 11.84KK167 pKa = 7.65PKK169 pKa = 10.34APRR172 pKa = 11.84TAMEE176 pKa = 4.23WAGLPPRR183 pKa = 11.84EE184 pKa = 4.12TLPPRR189 pKa = 11.84VVSSMGDD196 pKa = 3.44DD197 pKa = 5.3DD198 pKa = 6.88DD199 pKa = 6.34DD200 pKa = 6.45LPLCGPWTGRR210 pKa = 3.45

Molecular weight: 22.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5811

0

5811

2762008

67

4754

475.3

53.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.711 ± 0.034

1.116 ± 0.012

6.306 ± 0.025

6.094 ± 0.031

3.963 ± 0.022

5.685 ± 0.034

2.44 ± 0.013

5.193 ± 0.023

5.874 ± 0.031

8.817 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.137 ± 0.012

4.258 ± 0.02

5.468 ± 0.034

4.416 ± 0.028

4.961 ± 0.026

8.246 ± 0.039

5.758 ± 0.029

7.009 ± 0.03

1.22 ± 0.011

3.31 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski