Amino acid dipepetide frequency for Bacillus phage Gp

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.877AlaAla: 4.877 ± 0.998
0.495AlaCys: 0.495 ± 0.21
3.746AlaAsp: 3.746 ± 0.512
6.432AlaGlu: 6.432 ± 0.976
1.979AlaPhe: 1.979 ± 0.33
3.817AlaGly: 3.817 ± 0.524
0.636AlaHis: 0.636 ± 0.244
5.019AlaIle: 5.019 ± 0.602
5.231AlaLys: 5.231 ± 0.686
6.291AlaLeu: 6.291 ± 0.719
1.838AlaMet: 1.838 ± 0.38
3.958AlaAsn: 3.958 ± 0.501
1.414AlaPro: 1.414 ± 0.327
2.827AlaGln: 2.827 ± 0.422
2.615AlaArg: 2.615 ± 0.422
3.393AlaSer: 3.393 ± 0.499
4.17AlaThr: 4.17 ± 0.618
4.453AlaVal: 4.453 ± 0.733
0.636AlaTrp: 0.636 ± 0.223
2.403AlaTyr: 2.403 ± 0.38
0.0AlaXaa: 0.0 ± 0.0
Cys
0.495CysAla: 0.495 ± 0.194
0.071CysCys: 0.071 ± 0.073
1.06CysAsp: 1.06 ± 0.441
1.131CysGlu: 1.131 ± 0.274
0.495CysPhe: 0.495 ± 0.192
0.848CysGly: 0.848 ± 0.32
0.495CysHis: 0.495 ± 0.193
0.565CysIle: 0.565 ± 0.184
1.272CysLys: 1.272 ± 0.353
0.848CysLeu: 0.848 ± 0.267
0.0CysMet: 0.0 ± 0.0
0.565CysAsn: 0.565 ± 0.223
1.06CysPro: 1.06 ± 0.362
0.353CysGln: 0.353 ± 0.226
0.778CysArg: 0.778 ± 0.303
0.707CysSer: 0.707 ± 0.252
0.212CysThr: 0.212 ± 0.145
0.353CysVal: 0.353 ± 0.158
0.071CysTrp: 0.071 ± 0.082
0.212CysTyr: 0.212 ± 0.116
0.0CysXaa: 0.0 ± 0.0
Asp
3.04AspAla: 3.04 ± 0.508
0.707AspCys: 0.707 ± 0.216
3.464AspAsp: 3.464 ± 0.549
5.796AspGlu: 5.796 ± 0.74
3.464AspPhe: 3.464 ± 0.493
4.17AspGly: 4.17 ± 0.572
1.131AspHis: 1.131 ± 0.3
4.736AspIle: 4.736 ± 0.511
4.17AspLys: 4.17 ± 0.59
4.807AspLeu: 4.807 ± 0.544
1.414AspMet: 1.414 ± 0.373
2.827AspAsn: 2.827 ± 0.434
1.696AspPro: 1.696 ± 0.38
1.767AspGln: 1.767 ± 0.473
2.191AspArg: 2.191 ± 0.367
2.969AspSer: 2.969 ± 0.455
2.969AspThr: 2.969 ± 0.463
3.605AspVal: 3.605 ± 0.551
0.99AspTrp: 0.99 ± 0.249
2.403AspTyr: 2.403 ± 0.587
0.0AspXaa: 0.0 ± 0.0
Glu
5.938GluAla: 5.938 ± 0.684
0.848GluCys: 0.848 ± 0.294
4.524GluAsp: 4.524 ± 0.521
6.008GluGlu: 6.008 ± 0.739
2.615GluPhe: 2.615 ± 0.419
4.241GluGly: 4.241 ± 0.556
2.121GluHis: 2.121 ± 0.356
7.351GluIle: 7.351 ± 0.699
7.634GluLys: 7.634 ± 1.091
8.765GluLeu: 8.765 ± 0.913
3.181GluMet: 3.181 ± 0.511
4.877GluAsn: 4.877 ± 0.662
2.969GluPro: 2.969 ± 0.469
4.453GluGln: 4.453 ± 0.611
3.888GluArg: 3.888 ± 0.519
3.888GluSer: 3.888 ± 0.434
4.524GluThr: 4.524 ± 0.63
5.231GluVal: 5.231 ± 0.549
1.131GluTrp: 1.131 ± 0.251
3.393GluTyr: 3.393 ± 0.54
0.0GluXaa: 0.0 ± 0.0
Phe
2.262PheAla: 2.262 ± 0.454
0.636PheCys: 0.636 ± 0.24
2.191PheAsp: 2.191 ± 0.287
3.605PheGlu: 3.605 ± 0.534
1.272PhePhe: 1.272 ± 0.298
1.696PheGly: 1.696 ± 0.231
0.212PheHis: 0.212 ± 0.124
2.686PheIle: 2.686 ± 0.456
3.888PheLys: 3.888 ± 0.559
1.838PheLeu: 1.838 ± 0.386
1.414PheMet: 1.414 ± 0.308
2.474PheAsn: 2.474 ± 0.38
0.848PhePro: 0.848 ± 0.249
1.343PheGln: 1.343 ± 0.253
1.555PheArg: 1.555 ± 0.308
2.615PheSer: 2.615 ± 0.443
2.191PheThr: 2.191 ± 0.411
2.403PheVal: 2.403 ± 0.43
0.283PheTrp: 0.283 ± 0.14
2.262PheTyr: 2.262 ± 0.44
0.0PheXaa: 0.0 ± 0.0
Gly
3.888GlyAla: 3.888 ± 0.749
0.495GlyCys: 0.495 ± 0.208
3.534GlyAsp: 3.534 ± 0.41
4.595GlyGlu: 4.595 ± 0.515
2.827GlyPhe: 2.827 ± 0.474
3.676GlyGly: 3.676 ± 0.562
1.414GlyHis: 1.414 ± 0.319
4.17GlyIle: 4.17 ± 0.577
5.089GlyLys: 5.089 ± 0.554
5.443GlyLeu: 5.443 ± 0.637
1.202GlyMet: 1.202 ± 0.237
2.969GlyAsn: 2.969 ± 0.494
0.848GlyPro: 0.848 ± 0.255
1.555GlyGln: 1.555 ± 0.326
3.04GlyArg: 3.04 ± 0.502
2.898GlySer: 2.898 ± 0.409
3.04GlyThr: 3.04 ± 0.424
4.029GlyVal: 4.029 ± 0.658
1.343GlyTrp: 1.343 ± 0.293
2.333GlyTyr: 2.333 ± 0.41
0.0GlyXaa: 0.0 ± 0.0
His
1.202HisAla: 1.202 ± 0.309
0.283HisCys: 0.283 ± 0.151
0.99HisAsp: 0.99 ± 0.254
1.414HisGlu: 1.414 ± 0.294
0.707HisPhe: 0.707 ± 0.234
1.202HisGly: 1.202 ± 0.291
0.353HisHis: 0.353 ± 0.149
1.272HisIle: 1.272 ± 0.319
0.919HisLys: 0.919 ± 0.224
1.909HisLeu: 1.909 ± 0.372
0.283HisMet: 0.283 ± 0.138
0.99HisAsn: 0.99 ± 0.297
0.495HisPro: 0.495 ± 0.195
0.99HisGln: 0.99 ± 0.236
0.848HisArg: 0.848 ± 0.205
1.414HisSer: 1.414 ± 0.347
0.707HisThr: 0.707 ± 0.224
1.202HisVal: 1.202 ± 0.237
0.071HisTrp: 0.071 ± 0.072
0.778HisTyr: 0.778 ± 0.221
0.0HisXaa: 0.0 ± 0.0
Ile
6.15IleAla: 6.15 ± 0.679
0.707IleCys: 0.707 ± 0.232
5.443IleAsp: 5.443 ± 0.716
7.069IleGlu: 7.069 ± 0.776
3.04IlePhe: 3.04 ± 0.397
3.746IleGly: 3.746 ± 0.548
1.272IleHis: 1.272 ± 0.325
4.807IleIle: 4.807 ± 0.605
6.857IleLys: 6.857 ± 0.655
5.584IleLeu: 5.584 ± 0.637
1.767IleMet: 1.767 ± 0.356
3.605IleAsn: 3.605 ± 0.504
2.827IlePro: 2.827 ± 0.431
2.545IleGln: 2.545 ± 0.342
2.615IleArg: 2.615 ± 0.445
4.241IleSer: 4.241 ± 0.466
4.453IleThr: 4.453 ± 0.604
4.1IleVal: 4.1 ± 0.569
0.636IleTrp: 0.636 ± 0.21
2.262IleTyr: 2.262 ± 0.411
0.0IleXaa: 0.0 ± 0.0
Lys
6.998LysAla: 6.998 ± 0.852
1.06LysCys: 1.06 ± 0.34
4.029LysAsp: 4.029 ± 0.621
8.765LysGlu: 8.765 ± 1.01
2.121LysPhe: 2.121 ± 0.375
5.796LysGly: 5.796 ± 0.617
2.474LysHis: 2.474 ± 0.419
4.665LysIle: 4.665 ± 0.616
8.624LysLys: 8.624 ± 0.917
7.563LysLeu: 7.563 ± 0.896
1.979LysMet: 1.979 ± 0.429
4.877LysAsn: 4.877 ± 0.665
2.403LysPro: 2.403 ± 0.388
4.029LysGln: 4.029 ± 0.501
3.888LysArg: 3.888 ± 0.439
5.019LysSer: 5.019 ± 0.554
5.019LysThr: 5.019 ± 0.451
4.736LysVal: 4.736 ± 0.521
1.202LysTrp: 1.202 ± 0.292
2.686LysTyr: 2.686 ± 0.47
0.0LysXaa: 0.0 ± 0.0
Leu
5.16LeuAla: 5.16 ± 0.544
0.99LeuCys: 0.99 ± 0.387
6.15LeuAsp: 6.15 ± 0.639
5.938LeuGlu: 5.938 ± 0.569
2.121LeuPhe: 2.121 ± 0.4
4.807LeuGly: 4.807 ± 0.578
1.414LeuHis: 1.414 ± 0.333
4.736LeuIle: 4.736 ± 0.622
8.977LeuLys: 8.977 ± 0.809
6.362LeuLeu: 6.362 ± 0.882
2.615LeuMet: 2.615 ± 0.418
5.231LeuAsn: 5.231 ± 0.542
2.615LeuPro: 2.615 ± 0.428
3.322LeuGln: 3.322 ± 0.453
3.888LeuArg: 3.888 ± 0.492
6.503LeuSer: 6.503 ± 0.632
5.726LeuThr: 5.726 ± 0.794
4.241LeuVal: 4.241 ± 0.567
0.636LeuTrp: 0.636 ± 0.203
2.121LeuTyr: 2.121 ± 0.413
0.0LeuXaa: 0.0 ± 0.0
Met
1.767MetAla: 1.767 ± 0.418
0.071MetCys: 0.071 ± 0.082
1.484MetAsp: 1.484 ± 0.33
2.333MetGlu: 2.333 ± 0.447
0.778MetPhe: 0.778 ± 0.245
1.202MetGly: 1.202 ± 0.304
0.283MetHis: 0.283 ± 0.132
2.262MetIle: 2.262 ± 0.367
2.757MetLys: 2.757 ± 0.562
1.696MetLeu: 1.696 ± 0.378
0.283MetMet: 0.283 ± 0.168
1.909MetAsn: 1.909 ± 0.326
0.99MetPro: 0.99 ± 0.192
1.272MetGln: 1.272 ± 0.289
1.343MetArg: 1.343 ± 0.408
2.615MetSer: 2.615 ± 0.488
1.767MetThr: 1.767 ± 0.255
1.555MetVal: 1.555 ± 0.329
0.212MetTrp: 0.212 ± 0.129
0.636MetTyr: 0.636 ± 0.209
0.0MetXaa: 0.0 ± 0.0
Asn
4.524AsnAla: 4.524 ± 0.628
0.283AsnCys: 0.283 ± 0.133
2.474AsnAsp: 2.474 ± 0.455
5.301AsnGlu: 5.301 ± 0.74
1.838AsnPhe: 1.838 ± 0.384
3.958AsnGly: 3.958 ± 0.591
0.848AsnHis: 0.848 ± 0.227
4.736AsnIle: 4.736 ± 0.644
4.241AsnLys: 4.241 ± 0.782
4.948AsnLeu: 4.948 ± 0.607
1.979AsnMet: 1.979 ± 0.432
3.04AsnAsn: 3.04 ± 0.573
1.555AsnPro: 1.555 ± 0.4
1.696AsnGln: 1.696 ± 0.373
2.474AsnArg: 2.474 ± 0.439
2.898AsnSer: 2.898 ± 0.486
2.969AsnThr: 2.969 ± 0.453
2.898AsnVal: 2.898 ± 0.553
0.848AsnTrp: 0.848 ± 0.19
1.767AsnTyr: 1.767 ± 0.349
0.0AsnXaa: 0.0 ± 0.0
Pro
1.272ProAla: 1.272 ± 0.326
0.495ProCys: 0.495 ± 0.214
1.272ProAsp: 1.272 ± 0.304
3.04ProGlu: 3.04 ± 0.562
1.343ProPhe: 1.343 ± 0.259
0.919ProGly: 0.919 ± 0.278
0.353ProHis: 0.353 ± 0.153
2.191ProIle: 2.191 ± 0.457
2.898ProLys: 2.898 ± 0.545
2.827ProLeu: 2.827 ± 0.418
0.848ProMet: 0.848 ± 0.187
1.343ProAsn: 1.343 ± 0.383
0.99ProPro: 0.99 ± 0.269
0.99ProGln: 0.99 ± 0.333
1.272ProArg: 1.272 ± 0.352
1.838ProSer: 1.838 ± 0.362
2.121ProThr: 2.121 ± 0.346
1.767ProVal: 1.767 ± 0.268
0.212ProTrp: 0.212 ± 0.13
1.414ProTyr: 1.414 ± 0.295
0.0ProXaa: 0.0 ± 0.0
Gln
2.191GlnAla: 2.191 ± 0.546
0.424GlnCys: 0.424 ± 0.19
2.403GlnAsp: 2.403 ± 0.43
3.393GlnGlu: 3.393 ± 0.461
1.272GlnPhe: 1.272 ± 0.267
2.05GlnGly: 2.05 ± 0.36
0.565GlnHis: 0.565 ± 0.198
2.615GlnIle: 2.615 ± 0.481
4.241GlnLys: 4.241 ± 0.566
3.322GlnLeu: 3.322 ± 0.574
1.484GlnMet: 1.484 ± 0.329
2.05GlnAsn: 2.05 ± 0.366
1.131GlnPro: 1.131 ± 0.291
2.757GlnGln: 2.757 ± 0.541
1.909GlnArg: 1.909 ± 0.328
2.262GlnSer: 2.262 ± 0.341
2.262GlnThr: 2.262 ± 0.37
2.333GlnVal: 2.333 ± 0.429
0.353GlnTrp: 0.353 ± 0.13
1.414GlnTyr: 1.414 ± 0.346
0.0GlnXaa: 0.0 ± 0.0
Arg
2.262ArgAla: 2.262 ± 0.417
0.919ArgCys: 0.919 ± 0.299
2.686ArgAsp: 2.686 ± 0.439
4.595ArgGlu: 4.595 ± 0.79
2.545ArgPhe: 2.545 ± 0.431
1.767ArgGly: 1.767 ± 0.307
1.06ArgHis: 1.06 ± 0.29
4.029ArgIle: 4.029 ± 0.587
3.464ArgLys: 3.464 ± 0.534
4.241ArgLeu: 4.241 ± 0.593
1.414ArgMet: 1.414 ± 0.303
1.838ArgAsn: 1.838 ± 0.343
0.707ArgPro: 0.707 ± 0.215
1.696ArgGln: 1.696 ± 0.39
2.121ArgArg: 2.121 ± 0.415
1.979ArgSer: 1.979 ± 0.292
1.838ArgThr: 1.838 ± 0.323
2.333ArgVal: 2.333 ± 0.428
0.707ArgTrp: 0.707 ± 0.222
2.05ArgTyr: 2.05 ± 0.361
0.0ArgXaa: 0.0 ± 0.0
Ser
3.676SerAla: 3.676 ± 0.549
0.424SerCys: 0.424 ± 0.191
3.181SerAsp: 3.181 ± 0.668
4.665SerGlu: 4.665 ± 0.653
2.757SerPhe: 2.757 ± 0.44
3.393SerGly: 3.393 ± 0.474
0.848SerHis: 0.848 ± 0.216
4.807SerIle: 4.807 ± 0.669
4.453SerLys: 4.453 ± 0.504
4.1SerLeu: 4.1 ± 0.551
1.767SerMet: 1.767 ± 0.308
4.383SerAsn: 4.383 ± 0.493
1.626SerPro: 1.626 ± 0.379
2.262SerGln: 2.262 ± 0.402
2.969SerArg: 2.969 ± 0.498
3.322SerSer: 3.322 ± 0.472
2.898SerThr: 2.898 ± 0.456
4.1SerVal: 4.1 ± 0.46
0.778SerTrp: 0.778 ± 0.232
2.05SerTyr: 2.05 ± 0.462
0.0SerXaa: 0.0 ± 0.0
Thr
3.605ThrAla: 3.605 ± 0.605
0.707ThrCys: 0.707 ± 0.25
2.827ThrAsp: 2.827 ± 0.473
3.817ThrGlu: 3.817 ± 0.429
2.333ThrPhe: 2.333 ± 0.361
4.029ThrGly: 4.029 ± 0.646
0.848ThrHis: 0.848 ± 0.3
4.665ThrIle: 4.665 ± 0.594
4.312ThrLys: 4.312 ± 0.569
4.383ThrLeu: 4.383 ± 0.593
1.979ThrMet: 1.979 ± 0.424
2.898ThrAsn: 2.898 ± 0.523
1.767ThrPro: 1.767 ± 0.318
2.545ThrGln: 2.545 ± 0.407
2.474ThrArg: 2.474 ± 0.339
3.817ThrSer: 3.817 ± 0.528
2.191ThrThr: 2.191 ± 0.468
3.04ThrVal: 3.04 ± 0.521
0.495ThrTrp: 0.495 ± 0.175
1.838ThrTyr: 1.838 ± 0.392
0.0ThrXaa: 0.0 ± 0.0
Val
3.181ValAla: 3.181 ± 0.536
1.343ValCys: 1.343 ± 0.375
3.817ValAsp: 3.817 ± 0.499
5.089ValGlu: 5.089 ± 0.608
1.979ValPhe: 1.979 ± 0.348
3.676ValGly: 3.676 ± 0.585
0.707ValHis: 0.707 ± 0.261
4.383ValIle: 4.383 ± 0.561
5.443ValLys: 5.443 ± 0.563
5.019ValLeu: 5.019 ± 0.568
0.919ValMet: 0.919 ± 0.256
3.393ValAsn: 3.393 ± 0.756
2.121ValPro: 2.121 ± 0.479
1.909ValGln: 1.909 ± 0.327
1.979ValArg: 1.979 ± 0.313
3.605ValSer: 3.605 ± 0.562
3.817ValThr: 3.817 ± 0.592
4.241ValVal: 4.241 ± 0.545
0.495ValTrp: 0.495 ± 0.222
2.191ValTyr: 2.191 ± 0.429
0.0ValXaa: 0.0 ± 0.0
Trp
1.06TrpAla: 1.06 ± 0.341
0.0TrpCys: 0.0 ± 0.0
0.424TrpAsp: 0.424 ± 0.147
1.343TrpGlu: 1.343 ± 0.296
0.707TrpPhe: 0.707 ± 0.21
0.636TrpGly: 0.636 ± 0.212
0.283TrpHis: 0.283 ± 0.149
0.848TrpIle: 0.848 ± 0.26
1.202TrpLys: 1.202 ± 0.255
0.919TrpLeu: 0.919 ± 0.237
0.071TrpMet: 0.071 ± 0.082
0.283TrpAsn: 0.283 ± 0.126
0.141TrpPro: 0.141 ± 0.11
0.565TrpGln: 0.565 ± 0.216
0.636TrpArg: 0.636 ± 0.22
0.495TrpSer: 0.495 ± 0.156
0.495TrpThr: 0.495 ± 0.21
0.565TrpVal: 0.565 ± 0.222
0.141TrpTrp: 0.141 ± 0.105
0.778TrpTyr: 0.778 ± 0.297
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.686TyrAla: 2.686 ± 0.402
0.636TyrCys: 0.636 ± 0.221
2.615TyrAsp: 2.615 ± 0.409
3.252TyrGlu: 3.252 ± 0.498
1.484TyrPhe: 1.484 ± 0.306
2.757TyrGly: 2.757 ± 0.521
0.707TyrHis: 0.707 ± 0.213
3.252TyrIle: 3.252 ± 0.589
2.262TyrLys: 2.262 ± 0.388
2.686TyrLeu: 2.686 ± 0.435
0.707TyrMet: 0.707 ± 0.203
1.767TyrAsn: 1.767 ± 0.372
1.202TyrPro: 1.202 ± 0.302
1.555TyrGln: 1.555 ± 0.37
1.767TyrArg: 1.767 ± 0.376
1.979TyrSer: 1.979 ± 0.388
1.202TyrThr: 1.202 ± 0.267
2.121TyrVal: 2.121 ± 0.391
0.353TyrTrp: 0.353 ± 0.175
1.484TyrTyr: 1.484 ± 0.346
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 85 proteins (14148 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski