Paraburkholderia megapolitana

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Paraburkholderia

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6692 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I3DVB0|A0A1I3DVB0_9BURK Type IV pilus biogenesis protein PilP OS=Paraburkholderia megapolitana OX=420953 GN=SAMN05192543_101544 PE=4 SV=1
MM1 pKa = 7.49SSAAVQGYY9 pKa = 7.17STNPMDD15 pKa = 4.22YY16 pKa = 10.67LSNSNSYY23 pKa = 11.06GYY25 pKa = 10.71GSSDD29 pKa = 2.79ISGEE33 pKa = 4.13SGGVNGLNGNNSGTGMNEE51 pKa = 3.63QQILQIIEE59 pKa = 4.6EE60 pKa = 4.59LLQMLQQVLQNSDD73 pKa = 3.4GDD75 pKa = 4.13DD76 pKa = 3.77SGGGAPPVGSGSSPGGSSNPFPQDD100 pKa = 3.18YY101 pKa = 10.94SSAPPVGSGGNAPPLANSQSPATNGAPPVSGNAPPVTSNAPPPPSNPPPVNSGGNNSGNNGAPPAGSSTQTTNNPPPPANGSSGSSSGPTMFGLSTAPQTTALSQSQGQQVADD214 pKa = 3.34QYY216 pKa = 11.87VNNLMSDD223 pKa = 4.32FGLTKK228 pKa = 10.1PQAQGIVANLWHH240 pKa = 7.01EE241 pKa = 4.35SGGMNSGINQGGQIGAPTGNNADD264 pKa = 4.49DD265 pKa = 3.76NANGYY270 pKa = 10.39GIAQWGGSRR279 pKa = 11.84KK280 pKa = 9.74EE281 pKa = 3.93GLEE284 pKa = 4.1AYY286 pKa = 9.73AKK288 pKa = 10.65QNGLDD293 pKa = 3.87PSSEE297 pKa = 3.88AANYY301 pKa = 10.17GYY303 pKa = 10.68LKK305 pKa = 10.51QEE307 pKa = 4.15LQTTQSGAISAVKK320 pKa = 9.01GTSNAQDD327 pKa = 3.16ATSAFMTSFEE337 pKa = 4.54KK338 pKa = 10.76PSDD341 pKa = 3.68PEE343 pKa = 4.02LASRR347 pKa = 11.84LADD350 pKa = 3.62LSLVQGG356 pKa = 3.98

Molecular weight: 35.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I3G0T1|A0A1I3G0T1_9BURK Protoheme IX farnesyltransferase OS=Paraburkholderia megapolitana OX=420953 GN=ctaB PE=3 SV=1
MM1 pKa = 7.24NRR3 pKa = 11.84QFKK6 pKa = 7.92MTTIAMCVSSLIALSGCGSTVTKK29 pKa = 10.2PLGSGSGGGTIGTGGGGGGTGTGGGGTGTGGGGGKK64 pKa = 8.0TPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTLRR123 pKa = 11.84RR124 pKa = 11.84RR125 pKa = 11.84RR126 pKa = 11.84RR127 pKa = 11.84PLQLLPRR134 pKa = 11.84RR135 pKa = 11.84RR136 pKa = 11.84RR137 pKa = 11.84RR138 pKa = 11.84RR139 pKa = 11.84PRR141 pKa = 11.84LLRR144 pKa = 11.84RR145 pKa = 11.84LLRR148 pKa = 11.84RR149 pKa = 11.84RR150 pKa = 11.84HH151 pKa = 5.26RR152 pKa = 11.84RR153 pKa = 11.84LLRR156 pKa = 11.84RR157 pKa = 11.84RR158 pKa = 11.84RR159 pKa = 11.84LLRR162 pKa = 11.84RR163 pKa = 11.84RR164 pKa = 11.84LRR166 pKa = 11.84RR167 pKa = 11.84LLRR170 pKa = 11.84RR171 pKa = 11.84PLRR174 pKa = 11.84RR175 pKa = 11.84LRR177 pKa = 11.84LRR179 pKa = 11.84RR180 pKa = 11.84RR181 pKa = 11.84LTPTPSATSSRR192 pKa = 11.84TPATSSPRR200 pKa = 11.84LARR203 pKa = 11.84LSPRR207 pKa = 11.84SAARR211 pKa = 11.84SVSRR215 pKa = 11.84RR216 pKa = 11.84FPAATPRR223 pKa = 11.84RR224 pKa = 11.84RR225 pKa = 11.84LRR227 pKa = 11.84SATSSPTSAPVSLRR241 pKa = 11.84SAMEE245 pKa = 3.99RR246 pKa = 11.84PTVSASSARR255 pKa = 11.84LLTRR259 pKa = 11.84SASPSPAQAMSSTTPARR276 pKa = 11.84PSTTLGSS283 pKa = 3.55

Molecular weight: 30.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6692

0

6692

2228779

25

5663

333.1

36.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.541 ± 0.044

0.89 ± 0.009

5.504 ± 0.026

4.854 ± 0.033

3.686 ± 0.018

8.229 ± 0.045

2.351 ± 0.015

4.732 ± 0.024

2.826 ± 0.025

10.321 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.215 ± 0.014

2.909 ± 0.027

5.138 ± 0.024

3.694 ± 0.02

6.723 ± 0.036

5.895 ± 0.029

5.836 ± 0.037

7.782 ± 0.029

1.405 ± 0.011

2.471 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski