Skermania phage SPI1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N7C8T0|A0A0N7C8T0_9CAUD Uncharacterized protein OS=Skermania phage SPI1 OX=1636544 GN=SPI1_46 PE=4 SV=1
MM1 pKa = 7.7AIDD4 pKa = 3.77PLIYY8 pKa = 9.62EE9 pKa = 5.03APPLPTQGYY18 pKa = 8.6GLYY21 pKa = 10.43AAARR25 pKa = 11.84LIDD28 pKa = 3.66TGDD31 pKa = 3.35TSRR34 pKa = 11.84EE35 pKa = 3.93LLAGTDD41 pKa = 3.26IHH43 pKa = 7.5SVNCDD48 pKa = 3.73LGSGTYY54 pKa = 8.8GTDD57 pKa = 3.16LCEE60 pKa = 4.01TPPAVKK66 pKa = 9.74TPGTPARR73 pKa = 4.19

Molecular weight: 7.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N7C8R5|A0A0N7C8R5_9CAUD Uncharacterized protein OS=Skermania phage SPI1 OX=1636544 GN=SPI1_56 PE=4 SV=1
MM1 pKa = 7.43NIDD4 pKa = 3.46GYY6 pKa = 7.48MTRR9 pKa = 11.84TDD11 pKa = 3.02VAKK14 pKa = 10.47FVGVKK19 pKa = 9.56PDD21 pKa = 3.21SLSRR25 pKa = 11.84SNLPPPNLVVGRR37 pKa = 11.84SRR39 pKa = 11.84LWTRR43 pKa = 11.84EE44 pKa = 3.9VIEE47 pKa = 4.08EE48 pKa = 3.96WAAARR53 pKa = 11.84PGIGGRR59 pKa = 11.84WPDD62 pKa = 3.66PDD64 pKa = 2.86GTRR67 pKa = 11.84YY68 pKa = 9.69KK69 pKa = 10.69RR70 pKa = 11.84RR71 pKa = 11.84TT72 pKa = 3.39

Molecular weight: 8.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

17200

42

1800

256.7

27.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.07 ± 0.454

0.919 ± 0.146

7.599 ± 0.255

5.006 ± 0.217

1.843 ± 0.179

8.483 ± 0.258

1.762 ± 0.194

4.262 ± 0.229

1.651 ± 0.208

8.698 ± 0.296

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.913 ± 0.101

1.494 ± 0.146

6.25 ± 0.253

2.523 ± 0.255

8.238 ± 0.505

5.913 ± 0.222

7.395 ± 0.333

8.302 ± 0.255

2.198 ± 0.197

2.483 ± 0.216
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski