Amino acid dipepetide frequency for Klebsiella phage KN4-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.076AlaAla: 8.076 ± 1.225
0.655AlaCys: 0.655 ± 0.24
6.875AlaAsp: 6.875 ± 0.982
5.675AlaGlu: 5.675 ± 1.064
3.383AlaPhe: 3.383 ± 0.497
7.203AlaGly: 7.203 ± 1.396
1.2AlaHis: 1.2 ± 0.216
3.929AlaIle: 3.929 ± 0.589
6.002AlaLys: 6.002 ± 0.542
7.967AlaLeu: 7.967 ± 1.101
3.056AlaMet: 3.056 ± 0.808
4.475AlaAsn: 4.475 ± 0.584
2.51AlaPro: 2.51 ± 0.397
3.82AlaGln: 3.82 ± 0.712
4.911AlaArg: 4.911 ± 0.635
4.911AlaSer: 4.911 ± 0.842
3.929AlaThr: 3.929 ± 0.884
5.784AlaVal: 5.784 ± 0.722
1.31AlaTrp: 1.31 ± 0.427
3.056AlaTyr: 3.056 ± 0.532
0.0AlaXaa: 0.0 ± 0.0
Cys
0.873CysAla: 0.873 ± 0.297
0.109CysCys: 0.109 ± 0.117
0.655CysAsp: 0.655 ± 0.407
0.764CysGlu: 0.764 ± 0.284
0.437CysPhe: 0.437 ± 0.25
0.764CysGly: 0.764 ± 0.233
0.764CysHis: 0.764 ± 0.371
0.437CysIle: 0.437 ± 0.202
0.437CysLys: 0.437 ± 0.22
0.764CysLeu: 0.764 ± 0.243
0.109CysMet: 0.109 ± 0.091
0.327CysAsn: 0.327 ± 0.185
0.327CysPro: 0.327 ± 0.17
0.764CysGln: 0.764 ± 0.391
1.091CysArg: 1.091 ± 0.494
0.546CysSer: 0.546 ± 0.312
0.327CysThr: 0.327 ± 0.176
0.982CysVal: 0.982 ± 0.288
0.218CysTrp: 0.218 ± 0.151
0.218CysTyr: 0.218 ± 0.16
0.0CysXaa: 0.0 ± 0.0
Asp
5.238AspAla: 5.238 ± 0.705
0.546AspCys: 0.546 ± 0.293
4.038AspAsp: 4.038 ± 0.687
4.147AspGlu: 4.147 ± 0.572
2.947AspPhe: 2.947 ± 0.553
6.548AspGly: 6.548 ± 0.592
1.091AspHis: 1.091 ± 0.325
3.274AspIle: 3.274 ± 0.621
4.038AspLys: 4.038 ± 0.712
3.711AspLeu: 3.711 ± 0.63
1.637AspMet: 1.637 ± 0.391
2.401AspAsn: 2.401 ± 0.47
3.492AspPro: 3.492 ± 0.7
2.183AspGln: 2.183 ± 0.536
3.165AspArg: 3.165 ± 0.66
3.82AspSer: 3.82 ± 0.466
4.147AspThr: 4.147 ± 0.575
4.475AspVal: 4.475 ± 0.594
0.764AspTrp: 0.764 ± 0.358
2.619AspTyr: 2.619 ± 0.41
0.0AspXaa: 0.0 ± 0.0
Glu
6.875GluAla: 6.875 ± 1.127
0.982GluCys: 0.982 ± 0.405
4.475GluAsp: 4.475 ± 0.849
6.221GluGlu: 6.221 ± 1.696
2.619GluPhe: 2.619 ± 0.455
5.784GluGly: 5.784 ± 0.915
1.964GluHis: 1.964 ± 0.543
2.51GluIle: 2.51 ± 0.476
2.837GluLys: 2.837 ± 0.709
6.33GluLeu: 6.33 ± 1.011
0.982GluMet: 0.982 ± 0.537
2.837GluAsn: 2.837 ± 0.406
2.619GluPro: 2.619 ± 0.829
2.947GluGln: 2.947 ± 0.532
3.492GluArg: 3.492 ± 0.604
4.475GluSer: 4.475 ± 0.747
2.947GluThr: 2.947 ± 0.612
4.584GluVal: 4.584 ± 0.622
0.546GluTrp: 0.546 ± 0.279
3.165GluTyr: 3.165 ± 0.434
0.0GluXaa: 0.0 ± 0.0
Phe
2.728PheAla: 2.728 ± 0.49
0.327PheCys: 0.327 ± 0.222
2.947PheAsp: 2.947 ± 0.671
1.855PheGlu: 1.855 ± 0.401
0.764PhePhe: 0.764 ± 0.289
3.711PheGly: 3.711 ± 0.78
0.873PheHis: 0.873 ± 0.32
1.964PheIle: 1.964 ± 0.672
2.074PheLys: 2.074 ± 0.377
2.728PheLeu: 2.728 ± 0.515
0.982PheMet: 0.982 ± 0.371
2.292PheAsn: 2.292 ± 0.423
1.637PhePro: 1.637 ± 0.503
1.2PheGln: 1.2 ± 0.344
1.2PheArg: 1.2 ± 0.322
2.51PheSer: 2.51 ± 0.483
2.074PheThr: 2.074 ± 0.409
1.964PheVal: 1.964 ± 0.4
0.327PheTrp: 0.327 ± 0.159
0.982PheTyr: 0.982 ± 0.246
0.0PheXaa: 0.0 ± 0.0
Gly
7.312GlyAla: 7.312 ± 1.274
0.764GlyCys: 0.764 ± 0.24
5.675GlyAsp: 5.675 ± 0.69
5.566GlyGlu: 5.566 ± 0.825
3.165GlyPhe: 3.165 ± 0.419
6.221GlyGly: 6.221 ± 1.043
1.855GlyHis: 1.855 ± 0.692
5.129GlyIle: 5.129 ± 1.124
6.221GlyLys: 6.221 ± 0.98
6.33GlyLeu: 6.33 ± 0.746
1.746GlyMet: 1.746 ± 0.516
3.492GlyAsn: 3.492 ± 0.645
1.528GlyPro: 1.528 ± 0.519
3.492GlyGln: 3.492 ± 0.577
4.584GlyArg: 4.584 ± 0.623
6.33GlySer: 6.33 ± 1.131
4.802GlyThr: 4.802 ± 0.648
5.348GlyVal: 5.348 ± 0.909
1.637GlyTrp: 1.637 ± 0.424
3.492GlyTyr: 3.492 ± 0.554
0.0GlyXaa: 0.0 ± 0.0
His
1.746HisAla: 1.746 ± 0.301
0.546HisCys: 0.546 ± 0.193
1.091HisAsp: 1.091 ± 0.281
1.31HisGlu: 1.31 ± 0.511
0.764HisPhe: 0.764 ± 0.263
1.528HisGly: 1.528 ± 0.5
0.437HisHis: 0.437 ± 0.232
0.873HisIle: 0.873 ± 0.292
1.091HisLys: 1.091 ± 0.42
1.419HisLeu: 1.419 ± 0.39
0.655HisMet: 0.655 ± 0.205
0.327HisAsn: 0.327 ± 0.15
0.873HisPro: 0.873 ± 0.24
0.873HisGln: 0.873 ± 0.288
1.091HisArg: 1.091 ± 0.386
0.873HisSer: 0.873 ± 0.325
0.982HisThr: 0.982 ± 0.25
2.074HisVal: 2.074 ± 0.412
0.218HisTrp: 0.218 ± 0.134
1.2HisTyr: 1.2 ± 0.263
0.0HisXaa: 0.0 ± 0.0
Ile
4.693IleAla: 4.693 ± 0.467
0.873IleCys: 0.873 ± 0.239
3.383IleAsp: 3.383 ± 0.467
3.383IleGlu: 3.383 ± 0.511
0.655IlePhe: 0.655 ± 0.298
4.256IleGly: 4.256 ± 0.748
1.31IleHis: 1.31 ± 0.335
2.401IleIle: 2.401 ± 0.51
3.492IleLys: 3.492 ± 0.546
4.038IleLeu: 4.038 ± 0.588
0.982IleMet: 0.982 ± 0.35
2.183IleAsn: 2.183 ± 0.759
2.292IlePro: 2.292 ± 0.454
1.528IleGln: 1.528 ± 0.384
2.837IleArg: 2.837 ± 0.545
2.947IleSer: 2.947 ± 0.578
2.51IleThr: 2.51 ± 0.69
3.601IleVal: 3.601 ± 0.609
0.546IleTrp: 0.546 ± 0.248
1.528IleTyr: 1.528 ± 0.413
0.0IleXaa: 0.0 ± 0.0
Lys
6.766LysAla: 6.766 ± 1.096
0.546LysCys: 0.546 ± 0.255
3.383LysAsp: 3.383 ± 0.691
4.365LysGlu: 4.365 ± 0.682
1.964LysPhe: 1.964 ± 0.341
5.784LysGly: 5.784 ± 1.034
1.964LysHis: 1.964 ± 0.496
2.401LysIle: 2.401 ± 0.551
3.492LysLys: 3.492 ± 0.837
5.238LysLeu: 5.238 ± 0.739
1.419LysMet: 1.419 ± 0.374
3.056LysAsn: 3.056 ± 0.411
2.947LysPro: 2.947 ± 0.719
2.183LysGln: 2.183 ± 0.552
3.056LysArg: 3.056 ± 0.844
3.383LysSer: 3.383 ± 0.736
2.728LysThr: 2.728 ± 0.501
4.911LysVal: 4.911 ± 0.712
0.764LysTrp: 0.764 ± 0.309
1.637LysTyr: 1.637 ± 0.499
0.0LysXaa: 0.0 ± 0.0
Leu
7.967LeuAla: 7.967 ± 1.569
0.546LeuCys: 0.546 ± 0.315
4.693LeuAsp: 4.693 ± 0.519
6.875LeuGlu: 6.875 ± 1.234
2.401LeuPhe: 2.401 ± 0.543
5.566LeuGly: 5.566 ± 0.611
1.528LeuHis: 1.528 ± 0.428
3.165LeuIle: 3.165 ± 0.549
5.784LeuLys: 5.784 ± 0.672
7.312LeuLeu: 7.312 ± 1.097
2.183LeuMet: 2.183 ± 0.296
4.147LeuAsn: 4.147 ± 0.564
3.711LeuPro: 3.711 ± 0.591
3.711LeuGln: 3.711 ± 0.631
5.348LeuArg: 5.348 ± 0.725
5.457LeuSer: 5.457 ± 0.906
4.584LeuThr: 4.584 ± 0.752
5.566LeuVal: 5.566 ± 0.919
1.091LeuTrp: 1.091 ± 0.51
1.637LeuTyr: 1.637 ± 0.478
0.0LeuXaa: 0.0 ± 0.0
Met
3.056MetAla: 3.056 ± 0.595
0.109MetCys: 0.109 ± 0.127
1.855MetAsp: 1.855 ± 0.388
1.091MetGlu: 1.091 ± 0.384
0.764MetPhe: 0.764 ± 0.273
1.31MetGly: 1.31 ± 0.311
0.655MetHis: 0.655 ± 0.304
1.419MetIle: 1.419 ± 0.417
1.31MetLys: 1.31 ± 0.386
2.947MetLeu: 2.947 ± 0.431
0.655MetMet: 0.655 ± 0.251
1.091MetAsn: 1.091 ± 0.353
0.873MetPro: 0.873 ± 0.28
1.964MetGln: 1.964 ± 0.397
1.2MetArg: 1.2 ± 0.39
1.637MetSer: 1.637 ± 0.381
1.746MetThr: 1.746 ± 0.458
1.528MetVal: 1.528 ± 0.422
0.0MetTrp: 0.0 ± 0.0
0.218MetTyr: 0.218 ± 0.12
0.0MetXaa: 0.0 ± 0.0
Asn
3.601AsnAla: 3.601 ± 0.872
0.764AsnCys: 0.764 ± 0.29
1.637AsnAsp: 1.637 ± 0.365
3.165AsnGlu: 3.165 ± 0.835
1.419AsnPhe: 1.419 ± 0.393
4.038AsnGly: 4.038 ± 0.724
0.437AsnHis: 0.437 ± 0.211
2.728AsnIle: 2.728 ± 0.621
1.964AsnLys: 1.964 ± 0.515
3.929AsnLeu: 3.929 ± 0.67
1.091AsnMet: 1.091 ± 0.386
0.982AsnAsn: 0.982 ± 0.283
2.292AsnPro: 2.292 ± 0.483
1.2AsnGln: 1.2 ± 0.273
1.855AsnArg: 1.855 ± 0.639
2.619AsnSer: 2.619 ± 0.637
2.074AsnThr: 2.074 ± 0.488
3.492AsnVal: 3.492 ± 0.674
0.873AsnTrp: 0.873 ± 0.38
2.292AsnTyr: 2.292 ± 0.577
0.0AsnXaa: 0.0 ± 0.0
Pro
2.728ProAla: 2.728 ± 0.584
0.764ProCys: 0.764 ± 0.249
2.074ProAsp: 2.074 ± 0.377
4.365ProGlu: 4.365 ± 0.835
1.637ProPhe: 1.637 ± 0.354
2.728ProGly: 2.728 ± 0.556
0.546ProHis: 0.546 ± 0.226
1.528ProIle: 1.528 ± 0.453
1.855ProLys: 1.855 ± 0.463
3.274ProLeu: 3.274 ± 0.38
0.873ProMet: 0.873 ± 0.333
2.292ProAsn: 2.292 ± 0.467
1.528ProPro: 1.528 ± 0.419
0.982ProGln: 0.982 ± 0.281
1.964ProArg: 1.964 ± 0.349
2.292ProSer: 2.292 ± 0.384
1.855ProThr: 1.855 ± 0.521
3.165ProVal: 3.165 ± 0.45
0.982ProTrp: 0.982 ± 0.234
1.637ProTyr: 1.637 ± 0.53
0.0ProXaa: 0.0 ± 0.0
Gln
3.165GlnAla: 3.165 ± 0.557
0.218GlnCys: 0.218 ± 0.219
2.837GlnAsp: 2.837 ± 0.311
2.947GlnGlu: 2.947 ± 0.549
1.746GlnPhe: 1.746 ± 0.325
2.837GlnGly: 2.837 ± 0.616
0.437GlnHis: 0.437 ± 0.251
1.746GlnIle: 1.746 ± 0.515
2.837GlnLys: 2.837 ± 0.544
4.147GlnLeu: 4.147 ± 0.478
1.091GlnMet: 1.091 ± 0.407
1.2GlnAsn: 1.2 ± 0.333
2.292GlnPro: 2.292 ± 0.289
3.165GlnGln: 3.165 ± 0.554
2.183GlnArg: 2.183 ± 0.552
2.401GlnSer: 2.401 ± 0.582
1.31GlnThr: 1.31 ± 0.481
2.51GlnVal: 2.51 ± 0.441
0.655GlnTrp: 0.655 ± 0.23
1.419GlnTyr: 1.419 ± 0.579
0.0GlnXaa: 0.0 ± 0.0
Arg
5.129ArgAla: 5.129 ± 0.914
0.873ArgCys: 0.873 ± 0.32
3.383ArgAsp: 3.383 ± 0.565
3.929ArgGlu: 3.929 ± 0.633
1.528ArgPhe: 1.528 ± 0.349
4.802ArgGly: 4.802 ± 0.678
0.764ArgHis: 0.764 ± 0.278
3.492ArgIle: 3.492 ± 0.666
3.165ArgLys: 3.165 ± 0.494
4.038ArgLeu: 4.038 ± 0.798
1.419ArgMet: 1.419 ± 0.325
2.074ArgAsn: 2.074 ± 0.371
1.964ArgPro: 1.964 ± 0.333
2.51ArgGln: 2.51 ± 0.648
2.619ArgArg: 2.619 ± 0.379
3.274ArgSer: 3.274 ± 0.541
2.292ArgThr: 2.292 ± 0.464
3.274ArgVal: 3.274 ± 0.546
0.982ArgTrp: 0.982 ± 0.36
1.31ArgTyr: 1.31 ± 0.236
0.0ArgXaa: 0.0 ± 0.0
Ser
5.457SerAla: 5.457 ± 1.071
0.437SerCys: 0.437 ± 0.197
4.038SerAsp: 4.038 ± 0.657
3.601SerGlu: 3.601 ± 0.529
3.383SerPhe: 3.383 ± 0.581
6.439SerGly: 6.439 ± 1.254
1.637SerHis: 1.637 ± 0.326
3.601SerIle: 3.601 ± 0.677
4.038SerLys: 4.038 ± 0.529
4.475SerLeu: 4.475 ± 0.899
1.528SerMet: 1.528 ± 0.472
1.528SerAsn: 1.528 ± 0.547
1.528SerPro: 1.528 ± 0.399
3.056SerGln: 3.056 ± 0.559
3.056SerArg: 3.056 ± 0.512
3.056SerSer: 3.056 ± 0.482
3.383SerThr: 3.383 ± 0.794
4.584SerVal: 4.584 ± 0.589
0.655SerTrp: 0.655 ± 0.242
2.292SerTyr: 2.292 ± 0.617
0.0SerXaa: 0.0 ± 0.0
Thr
4.256ThrAla: 4.256 ± 0.811
0.764ThrCys: 0.764 ± 0.333
3.274ThrAsp: 3.274 ± 0.472
2.947ThrGlu: 2.947 ± 0.605
1.855ThrPhe: 1.855 ± 0.494
5.784ThrGly: 5.784 ± 0.967
0.873ThrHis: 0.873 ± 0.247
3.601ThrIle: 3.601 ± 0.656
3.711ThrLys: 3.711 ± 0.706
5.02ThrLeu: 5.02 ± 0.697
1.419ThrMet: 1.419 ± 0.264
1.637ThrAsn: 1.637 ± 0.501
2.401ThrPro: 2.401 ± 0.469
1.637ThrGln: 1.637 ± 0.448
1.964ThrArg: 1.964 ± 0.399
3.82ThrSer: 3.82 ± 1.083
2.728ThrThr: 2.728 ± 0.742
2.728ThrVal: 2.728 ± 0.651
0.327ThrTrp: 0.327 ± 0.153
1.528ThrTyr: 1.528 ± 0.361
0.0ThrXaa: 0.0 ± 0.0
Val
5.784ValAla: 5.784 ± 0.636
0.546ValCys: 0.546 ± 0.214
4.365ValAsp: 4.365 ± 0.691
4.256ValGlu: 4.256 ± 0.659
2.401ValPhe: 2.401 ± 0.658
5.02ValGly: 5.02 ± 0.575
0.982ValHis: 0.982 ± 0.354
3.165ValIle: 3.165 ± 0.711
4.365ValLys: 4.365 ± 0.596
5.893ValLeu: 5.893 ± 0.918
1.419ValMet: 1.419 ± 0.332
3.929ValAsn: 3.929 ± 0.864
2.947ValPro: 2.947 ± 0.538
1.964ValGln: 1.964 ± 0.454
4.256ValArg: 4.256 ± 0.653
5.129ValSer: 5.129 ± 1.136
4.584ValThr: 4.584 ± 0.639
5.348ValVal: 5.348 ± 0.85
0.764ValTrp: 0.764 ± 0.314
2.619ValTyr: 2.619 ± 0.546
0.0ValXaa: 0.0 ± 0.0
Trp
0.655TrpAla: 0.655 ± 0.211
0.437TrpCys: 0.437 ± 0.226
0.764TrpAsp: 0.764 ± 0.269
0.546TrpGlu: 0.546 ± 0.204
0.327TrpPhe: 0.327 ± 0.178
0.764TrpGly: 0.764 ± 0.231
0.218TrpHis: 0.218 ± 0.233
0.764TrpIle: 0.764 ± 0.317
1.637TrpLys: 1.637 ± 0.465
1.2TrpLeu: 1.2 ± 0.413
0.546TrpMet: 0.546 ± 0.233
0.437TrpAsn: 0.437 ± 0.222
0.218TrpPro: 0.218 ± 0.149
0.764TrpGln: 0.764 ± 0.267
0.764TrpArg: 0.764 ± 0.28
0.764TrpSer: 0.764 ± 0.415
0.873TrpThr: 0.873 ± 0.287
1.31TrpVal: 1.31 ± 0.427
0.327TrpTrp: 0.327 ± 0.177
0.218TrpTyr: 0.218 ± 0.133
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.619TyrAla: 2.619 ± 0.55
0.109TyrCys: 0.109 ± 0.107
2.837TyrAsp: 2.837 ± 0.527
2.183TyrGlu: 2.183 ± 0.509
0.982TyrPhe: 0.982 ± 0.239
3.601TyrGly: 3.601 ± 0.404
0.437TyrHis: 0.437 ± 0.241
1.31TyrIle: 1.31 ± 0.504
1.637TyrLys: 1.637 ± 0.398
2.51TyrLeu: 2.51 ± 0.459
1.528TyrMet: 1.528 ± 0.469
1.855TyrAsn: 1.855 ± 0.555
1.091TyrPro: 1.091 ± 0.328
1.2TyrGln: 1.2 ± 0.526
2.183TyrArg: 2.183 ± 0.382
1.528TyrSer: 1.528 ± 0.435
2.401TyrThr: 2.401 ± 0.708
2.51TyrVal: 2.51 ± 0.558
0.546TyrTrp: 0.546 ± 0.261
1.091TyrTyr: 1.091 ± 0.41
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 20 proteins (9164 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski