Amanita muscaria Koide BX008

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Agaricales; Amanitaceae; Amanita; Amanita muscaria

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17954 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C2RYL4|A0A0C2RYL4_AMAMU Uncharacterized protein OS=Amanita muscaria Koide BX008 OX=946122 GN=M378DRAFT_201107 PE=4 SV=1
MM1 pKa = 7.07STYY4 pKa = 9.61STASEE9 pKa = 4.19NSASDD14 pKa = 3.74ASPAPLPVPIPSTVEE29 pKa = 3.87VYY31 pKa = 10.87AVVPPGWGGPAWTDD45 pKa = 2.88IEE47 pKa = 4.55SAHH50 pKa = 6.84PADD53 pKa = 3.86SWQEE57 pKa = 3.87GLTTRR62 pKa = 11.84WDD64 pKa = 3.99TPSPDD69 pKa = 2.97SWSPIIPLDD78 pKa = 3.81PLVAPLTTPISPAPLPSPLLLPAPPLTQASPSPAPSPIPQYY119 pKa = 11.38VEE121 pKa = 4.4ALADD125 pKa = 3.59QLLEE129 pKa = 4.11AMEE132 pKa = 5.22DD133 pKa = 3.86GSDD136 pKa = 4.2KK137 pKa = 11.4EE138 pKa = 4.53NIPPRR143 pKa = 11.84AVTPYY148 pKa = 10.14PDD150 pKa = 4.04TPHH153 pKa = 7.31LLQSEE158 pKa = 5.01FYY160 pKa = 9.16ITT162 pKa = 3.83

Molecular weight: 17.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C2XQY7|A0A0C2XQY7_AMAMU SH3 domain-containing protein OS=Amanita muscaria Koide BX008 OX=946122 GN=M378DRAFT_183009 PE=3 SV=1
MM1 pKa = 6.6TTSSTSSASSSRR13 pKa = 11.84ATSIASSLRR22 pKa = 11.84ATSSASSSRR31 pKa = 11.84ATLTTSSTPSASSLRR46 pKa = 11.84ATLTTSSTPSASSSRR61 pKa = 11.84ATLTASSTSSASSLRR76 pKa = 11.84ATLTTSSTPASASSSRR92 pKa = 11.84ATSFASSLRR101 pKa = 11.84ATLTASSVTSSAPPLRR117 pKa = 11.84ATLTTSSASSTSSSRR132 pKa = 11.84ATSSATLTTSSTSSASSLTTKK153 pKa = 9.35STSFSTTSSTRR164 pKa = 11.84STTSPP169 pKa = 3.17

Molecular weight: 16.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

17947

7

17954

5887322

49

3848

327.9

36.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.692 ± 0.015

1.42 ± 0.008

5.588 ± 0.013

5.811 ± 0.02

3.851 ± 0.013

6.035 ± 0.019

2.682 ± 0.009

5.282 ± 0.016

4.833 ± 0.018

9.437 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.09 ± 0.008

3.802 ± 0.011

6.107 ± 0.023

3.949 ± 0.014

6.255 ± 0.016

8.701 ± 0.025

6.063 ± 0.013

6.228 ± 0.013

1.415 ± 0.006

2.76 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski