Hubei sobemo-like virus 6

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEM4|A0A1L3KEM4_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 6 OX=1923239 PE=4 SV=1
MM1 pKa = 7.83PDD3 pKa = 3.2WVVEE7 pKa = 4.34AYY9 pKa = 10.16FRR11 pKa = 11.84ARR13 pKa = 11.84LAQCSNSDD21 pKa = 3.09QRR23 pKa = 11.84YY24 pKa = 8.55EE25 pKa = 3.97KK26 pKa = 10.86LCWNRR31 pKa = 11.84MMCVVGPGAVLRR43 pKa = 11.84LPDD46 pKa = 3.96GQRR49 pKa = 11.84LRR51 pKa = 11.84QTCVGFMKK59 pKa = 10.58SGWLLTLSMNSAAQFFQHH77 pKa = 4.48VVAWRR82 pKa = 11.84RR83 pKa = 11.84SGYY86 pKa = 10.25DD87 pKa = 3.62LSSLPYY93 pKa = 8.03MWAMGDD99 pKa = 3.73DD100 pKa = 3.43MLLRR104 pKa = 11.84WEE106 pKa = 4.54EE107 pKa = 4.58GYY109 pKa = 11.09KK110 pKa = 10.37LEE112 pKa = 5.24PYY114 pKa = 10.47VAMLATTGCIVKK126 pKa = 9.79HH127 pKa = 5.47AVWSRR132 pKa = 11.84EE133 pKa = 3.54FAGYY137 pKa = 10.24AFKK140 pKa = 11.16GDD142 pKa = 4.0GVSPLYY148 pKa = 9.4PAKK151 pKa = 10.64HH152 pKa = 6.31KK153 pKa = 9.02FTLSYY158 pKa = 10.64VKK160 pKa = 10.73EE161 pKa = 4.11EE162 pKa = 4.26VEE164 pKa = 4.39QEE166 pKa = 4.09LLLSYY171 pKa = 10.53HH172 pKa = 7.1LLYY175 pKa = 11.13ALDD178 pKa = 4.32PNSWLPAVRR187 pKa = 11.84EE188 pKa = 4.08HH189 pKa = 7.03CRR191 pKa = 11.84FPVGRR196 pKa = 11.84MFRR199 pKa = 11.84MWAMGLIEE207 pKa = 6.14LEE209 pKa = 4.35MLPLMTRR216 pKa = 11.84VGCDD220 pKa = 2.96EE221 pKa = 3.99

Molecular weight: 25.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEM4|A0A1L3KEM4_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 6 OX=1923239 PE=4 SV=1
MM1 pKa = 7.08YY2 pKa = 10.2AVSMYY7 pKa = 10.86LATLLVWAVFMTYY20 pKa = 8.78TVVDD24 pKa = 5.21FFWDD28 pKa = 3.52WPVVRR33 pKa = 11.84QAVLRR38 pKa = 11.84DD39 pKa = 3.51RR40 pKa = 11.84AGKK43 pKa = 10.29ALFDD47 pKa = 3.86FCLGAVVLGIGFVLFFSSHH66 pKa = 7.82DD67 pKa = 3.24ILEE70 pKa = 4.62WIGRR74 pKa = 11.84GVEE77 pKa = 4.06TLVRR81 pKa = 11.84RR82 pKa = 11.84IWKK85 pKa = 9.4FCFARR90 pKa = 11.84ATARR94 pKa = 11.84VARR97 pKa = 11.84PDD99 pKa = 2.92KK100 pKa = 10.95SMRR103 pKa = 11.84RR104 pKa = 11.84VLEE107 pKa = 4.06TMKK110 pKa = 10.6EE111 pKa = 4.17GSDD114 pKa = 3.51LQTIPRR120 pKa = 11.84PACQLALYY128 pKa = 9.8DD129 pKa = 3.74YY130 pKa = 11.35DD131 pKa = 4.32SIEE134 pKa = 3.91RR135 pKa = 11.84QKK137 pKa = 11.25YY138 pKa = 7.83YY139 pKa = 10.73LGGAIHH145 pKa = 6.77VEE147 pKa = 4.24GGWLVSCTHH156 pKa = 6.7IVNQLRR162 pKa = 11.84NKK164 pKa = 9.76PYY166 pKa = 10.94ASVLDD171 pKa = 4.09PQGVEE176 pKa = 3.52QFYY179 pKa = 11.02SLEE182 pKa = 3.95GLEE185 pKa = 4.15WEE187 pKa = 4.86EE188 pKa = 3.84IGADD192 pKa = 3.45VSVAKK197 pKa = 10.55LGRR200 pKa = 11.84VFQHH204 pKa = 6.62LKK206 pKa = 6.69TAKK209 pKa = 9.05VGPVEE214 pKa = 3.8GSVYY218 pKa = 10.49VRR220 pKa = 11.84VHH222 pKa = 5.11SAKK225 pKa = 10.18GRR227 pKa = 11.84NNTSVGRR234 pKa = 11.84LTLDD238 pKa = 2.98SVTFGLLVYY247 pKa = 10.53DD248 pKa = 4.7GSTVAGFSGGAYY260 pKa = 9.25YY261 pKa = 10.71LANRR265 pKa = 11.84VVGIHH270 pKa = 6.55CGGGAINYY278 pKa = 7.26GYY280 pKa = 10.78SMEE283 pKa = 4.48WIMSVLARR291 pKa = 11.84RR292 pKa = 11.84GSVPEE297 pKa = 4.42AGGQYY302 pKa = 11.18ALRR305 pKa = 11.84NILRR309 pKa = 11.84SARR312 pKa = 11.84KK313 pKa = 9.24SDD315 pKa = 3.54VQVTSSGNPDD325 pKa = 3.13EE326 pKa = 4.45VQVRR330 pKa = 11.84VGGKK334 pKa = 7.97FWIVDD339 pKa = 3.49RR340 pKa = 11.84EE341 pKa = 4.31DD342 pKa = 3.94YY343 pKa = 10.98VDD345 pKa = 5.34LMEE348 pKa = 5.77DD349 pKa = 4.67DD350 pKa = 3.58KK351 pKa = 11.43FGWMFDD357 pKa = 3.65DD358 pKa = 4.43DD359 pKa = 4.44SEE361 pKa = 5.12RR362 pKa = 11.84EE363 pKa = 3.83EE364 pKa = 4.37RR365 pKa = 11.84NMRR368 pKa = 11.84KK369 pKa = 9.57RR370 pKa = 11.84FRR372 pKa = 11.84DD373 pKa = 3.22TFYY376 pKa = 11.19EE377 pKa = 3.88NDD379 pKa = 3.52RR380 pKa = 11.84EE381 pKa = 4.41AQGEE385 pKa = 4.37LFPEE389 pKa = 5.15GSVVSTLNGSATPSGTQEE407 pKa = 3.97LQGLIQSQIQNTIEE421 pKa = 3.93IQSLKK426 pKa = 10.51SQQQVMNGLEE436 pKa = 4.28QKK438 pKa = 11.03LEE440 pKa = 4.09TLDD443 pKa = 4.08DD444 pKa = 4.32LLRR447 pKa = 11.84SIPITGHH454 pKa = 6.09APLMDD459 pKa = 3.45MLEE462 pKa = 4.06SLEE465 pKa = 4.26RR466 pKa = 11.84KK467 pKa = 7.85VTSLSQSLSRR477 pKa = 11.84QTSSPLRR484 pKa = 11.84EE485 pKa = 4.62DD486 pKa = 3.74LSQVRR491 pKa = 11.84TVGNSTSLPCSEE503 pKa = 4.92PSTSRR508 pKa = 11.84VQPVSVNSKK517 pKa = 8.47RR518 pKa = 11.84TTPSVRR524 pKa = 11.84PWDD527 pKa = 3.69GMEE530 pKa = 4.11SDD532 pKa = 3.84LRR534 pKa = 11.84TYY536 pKa = 7.23VTWRR540 pKa = 11.84SSVDD544 pKa = 2.98ASRR547 pKa = 11.84KK548 pKa = 9.72DD549 pKa = 3.96FYY551 pKa = 11.73ALRR554 pKa = 11.84TEE556 pKa = 4.51FLVQHH561 pKa = 6.15MPHH564 pKa = 5.96LTIEE568 pKa = 4.07QRR570 pKa = 11.84AEE572 pKa = 3.81LVRR575 pKa = 11.84LEE577 pKa = 3.9KK578 pKa = 10.54ARR580 pKa = 11.84RR581 pKa = 11.84VNRR584 pKa = 11.84KK585 pKa = 7.91KK586 pKa = 8.01TQKK589 pKa = 10.17KK590 pKa = 9.72SSEE593 pKa = 3.79GKK595 pKa = 8.78QRR597 pKa = 11.84KK598 pKa = 5.78EE599 pKa = 3.47QNRR602 pKa = 11.84AIQQTLSSLL611 pKa = 3.68

Molecular weight: 68.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

832

221

611

416.0

47.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.25 ± 0.738

1.563 ± 0.821

5.048 ± 0.499

6.37 ± 0.018

3.726 ± 0.177

7.332 ± 0.279

1.683 ± 0.297

2.885 ± 1.013

4.087 ± 0.239

10.096 ± 1.086

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.486 ± 1.458

2.524 ± 0.365

3.726 ± 0.64

4.808 ± 0.84

8.053 ± 0.416

8.293 ± 1.234

4.808 ± 1.071

9.014 ± 0.677

2.524 ± 0.792

3.726 ± 0.64
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski