Streptomyces phage Intolerant

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TBM4|A0A5J6TBM4_9CAUD Uncharacterized protein OS=Streptomyces phage Intolerant OX=2601642 GN=50 PE=4 SV=1
MM1 pKa = 7.18IPKK4 pKa = 9.6YY5 pKa = 10.2LAGYY9 pKa = 9.36AVAAAALGALLTACGPDD26 pKa = 4.24DD27 pKa = 3.82GTEE30 pKa = 4.16PSPSPAPAPSAAQSSEE46 pKa = 4.0TALSDD51 pKa = 3.72SDD53 pKa = 4.81LFLAAARR60 pKa = 11.84AVAPDD65 pKa = 4.06LQAVPDD71 pKa = 4.2SDD73 pKa = 6.34LIDD76 pKa = 4.62LGQSVCGAFDD86 pKa = 3.76AGADD90 pKa = 3.45TATIGVTMITSGLDD104 pKa = 3.16ATASGAVVGAATSTLCPEE122 pKa = 4.77HH123 pKa = 7.03EE124 pKa = 4.16DD125 pKa = 3.54AARR128 pKa = 11.84GEE130 pKa = 4.27

Molecular weight: 12.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TE10|A0A5J6TE10_9CAUD Uncharacterized protein OS=Streptomyces phage Intolerant OX=2601642 GN=54 PE=4 SV=1
MM1 pKa = 7.17SRR3 pKa = 11.84KK4 pKa = 8.85NRR6 pKa = 11.84RR7 pKa = 11.84ARR9 pKa = 11.84LIASGYY15 pKa = 9.08SPTMLDD21 pKa = 2.94RR22 pKa = 11.84LEE24 pKa = 5.04RR25 pKa = 11.84EE26 pKa = 4.74GIPRR30 pKa = 11.84PPAAKK35 pKa = 9.34PKK37 pKa = 9.42EE38 pKa = 4.11FVPSGVLAMVQHH50 pKa = 6.63PEE52 pKa = 3.53RR53 pKa = 11.84GRR55 pKa = 11.84YY56 pKa = 6.64FQRR59 pKa = 11.84VKK61 pKa = 10.49PATTGITLHH70 pKa = 6.28AVWVSDD76 pKa = 4.07DD77 pKa = 3.38AHH79 pKa = 6.72PLGGYY84 pKa = 9.73LGSLTLHH91 pKa = 6.04RR92 pKa = 11.84RR93 pKa = 11.84VNLRR97 pKa = 11.84KK98 pKa = 9.93DD99 pKa = 3.54DD100 pKa = 3.69VPRR103 pKa = 11.84WIRR106 pKa = 11.84DD107 pKa = 3.14RR108 pKa = 11.84PLYY111 pKa = 10.48EE112 pKa = 3.91FTKK115 pKa = 9.78EE116 pKa = 3.89QPRR119 pKa = 3.55

Molecular weight: 13.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

16585

41

1366

212.6

23.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.071 ± 0.571

1.073 ± 0.161

6.144 ± 0.305

6.373 ± 0.405

2.93 ± 0.167

9.159 ± 0.378

1.724 ± 0.145

3.925 ± 0.236

5.68 ± 0.623

7.483 ± 0.375

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.478 ± 0.127

2.852 ± 0.25

5.523 ± 0.239

3.377 ± 0.17

6.313 ± 0.347

5.053 ± 0.274

6.017 ± 0.243

6.886 ± 0.235

2.309 ± 0.191

2.629 ± 0.19
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski