Amino acid dipepetide frequency for Escherichia virus TLS

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.973AlaAla: 5.973 ± 0.842
0.649AlaCys: 0.649 ± 0.224
4.48AlaAsp: 4.48 ± 0.56
5.713AlaGlu: 5.713 ± 0.634
3.181AlaPhe: 3.181 ± 0.494
5.973AlaGly: 5.973 ± 0.724
0.714AlaHis: 0.714 ± 0.243
4.285AlaIle: 4.285 ± 0.499
7.077AlaLys: 7.077 ± 0.974
7.985AlaLeu: 7.985 ± 0.811
2.597AlaMet: 2.597 ± 0.397
3.636AlaAsn: 3.636 ± 0.532
1.753AlaPro: 1.753 ± 0.299
4.674AlaGln: 4.674 ± 0.606
4.609AlaArg: 4.609 ± 0.578
4.09AlaSer: 4.09 ± 0.449
3.441AlaThr: 3.441 ± 0.516
5.583AlaVal: 5.583 ± 0.672
1.169AlaTrp: 1.169 ± 0.216
3.246AlaTyr: 3.246 ± 0.483
0.0AlaXaa: 0.0 ± 0.0
Cys
1.298CysAla: 1.298 ± 0.306
0.13CysCys: 0.13 ± 0.093
0.974CysAsp: 0.974 ± 0.237
0.649CysGlu: 0.649 ± 0.233
0.454CysPhe: 0.454 ± 0.19
1.298CysGly: 1.298 ± 0.295
0.39CysHis: 0.39 ± 0.169
0.454CysIle: 0.454 ± 0.143
0.844CysLys: 0.844 ± 0.238
1.039CysLeu: 1.039 ± 0.286
0.649CysMet: 0.649 ± 0.189
0.519CysAsn: 0.519 ± 0.177
0.325CysPro: 0.325 ± 0.118
0.13CysGln: 0.13 ± 0.098
0.779CysArg: 0.779 ± 0.277
0.584CysSer: 0.584 ± 0.147
0.649CysThr: 0.649 ± 0.182
1.039CysVal: 1.039 ± 0.27
0.649CysTrp: 0.649 ± 0.244
0.454CysTyr: 0.454 ± 0.155
0.0CysXaa: 0.0 ± 0.0
Asp
4.609AspAla: 4.609 ± 0.596
0.649AspCys: 0.649 ± 0.186
3.181AspAsp: 3.181 ± 0.507
4.48AspGlu: 4.48 ± 0.396
2.078AspPhe: 2.078 ± 0.341
5.389AspGly: 5.389 ± 0.751
0.974AspHis: 0.974 ± 0.226
3.636AspIle: 3.636 ± 0.386
4.674AspLys: 4.674 ± 0.421
4.999AspLeu: 4.999 ± 0.513
1.428AspMet: 1.428 ± 0.323
3.571AspAsn: 3.571 ± 0.397
2.467AspPro: 2.467 ± 0.348
1.558AspGln: 1.558 ± 0.318
3.181AspArg: 3.181 ± 0.543
3.246AspSer: 3.246 ± 0.448
3.441AspThr: 3.441 ± 0.446
3.181AspVal: 3.181 ± 0.47
0.844AspTrp: 0.844 ± 0.194
3.051AspTyr: 3.051 ± 0.381
0.0AspXaa: 0.0 ± 0.0
Glu
4.999GluAla: 4.999 ± 0.603
1.169GluCys: 1.169 ± 0.246
2.792GluAsp: 2.792 ± 0.454
5.259GluGlu: 5.259 ± 0.839
4.48GluPhe: 4.48 ± 0.503
3.246GluGly: 3.246 ± 0.414
1.753GluHis: 1.753 ± 0.341
4.804GluIle: 4.804 ± 0.556
4.999GluLys: 4.999 ± 0.542
5.389GluLeu: 5.389 ± 0.651
2.792GluMet: 2.792 ± 0.366
4.35GluAsn: 4.35 ± 0.449
1.818GluPro: 1.818 ± 0.411
3.895GluGln: 3.895 ± 0.521
3.571GluArg: 3.571 ± 0.504
4.48GluSer: 4.48 ± 0.443
3.766GluThr: 3.766 ± 0.479
4.545GluVal: 4.545 ± 0.571
0.844GluTrp: 0.844 ± 0.255
3.441GluTyr: 3.441 ± 0.435
0.0GluXaa: 0.0 ± 0.0
Phe
2.597PheAla: 2.597 ± 0.432
0.584PheCys: 0.584 ± 0.204
3.051PheAsp: 3.051 ± 0.494
2.402PheGlu: 2.402 ± 0.364
1.363PhePhe: 1.363 ± 0.299
3.181PheGly: 3.181 ± 0.55
0.909PheHis: 0.909 ± 0.282
2.467PheIle: 2.467 ± 0.427
3.311PheLys: 3.311 ± 0.434
2.207PheLeu: 2.207 ± 0.32
1.169PheMet: 1.169 ± 0.299
2.922PheAsn: 2.922 ± 0.348
1.688PhePro: 1.688 ± 0.367
1.169PheGln: 1.169 ± 0.288
1.688PheArg: 1.688 ± 0.326
2.467PheSer: 2.467 ± 0.628
2.532PheThr: 2.532 ± 0.472
2.727PheVal: 2.727 ± 0.305
0.519PheTrp: 0.519 ± 0.159
1.298PheTyr: 1.298 ± 0.284
0.0PheXaa: 0.0 ± 0.0
Gly
5.194GlyAla: 5.194 ± 0.701
1.169GlyCys: 1.169 ± 0.257
3.895GlyAsp: 3.895 ± 0.554
5.583GlyGlu: 5.583 ± 0.561
2.922GlyPhe: 2.922 ± 0.446
6.233GlyGly: 6.233 ± 0.73
1.039GlyHis: 1.039 ± 0.227
4.22GlyIle: 4.22 ± 0.428
5.648GlyLys: 5.648 ± 0.599
4.35GlyLeu: 4.35 ± 0.412
2.792GlyMet: 2.792 ± 0.306
3.83GlyAsn: 3.83 ± 0.348
0.0GlyPro: 0.0 ± 0.0
2.078GlyGln: 2.078 ± 0.449
2.597GlyArg: 2.597 ± 0.403
4.869GlySer: 4.869 ± 0.633
3.766GlyThr: 3.766 ± 0.628
5.518GlyVal: 5.518 ± 0.524
1.428GlyTrp: 1.428 ± 0.235
3.83GlyTyr: 3.83 ± 0.376
0.0GlyXaa: 0.0 ± 0.0
His
1.298HisAla: 1.298 ± 0.321
0.195HisCys: 0.195 ± 0.104
1.363HisAsp: 1.363 ± 0.339
1.039HisGlu: 1.039 ± 0.267
0.779HisPhe: 0.779 ± 0.263
1.623HisGly: 1.623 ± 0.31
0.519HisHis: 0.519 ± 0.287
1.169HisIle: 1.169 ± 0.307
1.493HisLys: 1.493 ± 0.42
1.234HisLeu: 1.234 ± 0.362
0.519HisMet: 0.519 ± 0.198
0.779HisAsn: 0.779 ± 0.242
0.584HisPro: 0.584 ± 0.21
0.714HisGln: 0.714 ± 0.232
1.169HisArg: 1.169 ± 0.323
1.169HisSer: 1.169 ± 0.325
0.909HisThr: 0.909 ± 0.24
1.298HisVal: 1.298 ± 0.301
0.195HisTrp: 0.195 ± 0.114
0.649HisTyr: 0.649 ± 0.237
0.0HisXaa: 0.0 ± 0.0
Ile
6.557IleAla: 6.557 ± 0.702
1.104IleCys: 1.104 ± 0.286
4.609IleAsp: 4.609 ± 0.417
5.064IleGlu: 5.064 ± 0.535
2.207IlePhe: 2.207 ± 0.311
3.571IleGly: 3.571 ± 0.486
1.428IleHis: 1.428 ± 0.324
3.96IleIle: 3.96 ± 0.575
5.908IleLys: 5.908 ± 0.492
3.311IleLeu: 3.311 ± 0.513
2.337IleMet: 2.337 ± 0.42
3.441IleAsn: 3.441 ± 0.527
2.402IlePro: 2.402 ± 0.34
1.948IleGln: 1.948 ± 0.405
2.857IleArg: 2.857 ± 0.478
3.96IleSer: 3.96 ± 0.415
4.48IleThr: 4.48 ± 0.609
4.35IleVal: 4.35 ± 0.48
0.779IleTrp: 0.779 ± 0.231
2.142IleTyr: 2.142 ± 0.391
0.0IleXaa: 0.0 ± 0.0
Lys
7.661LysAla: 7.661 ± 0.758
0.519LysCys: 0.519 ± 0.172
4.739LysAsp: 4.739 ± 0.586
6.752LysGlu: 6.752 ± 0.789
2.402LysPhe: 2.402 ± 0.399
3.96LysGly: 3.96 ± 0.515
1.428LysHis: 1.428 ± 0.331
4.674LysIle: 4.674 ± 0.417
4.674LysLys: 4.674 ± 0.672
5.129LysLeu: 5.129 ± 0.509
3.571LysMet: 3.571 ± 0.568
4.09LysAsn: 4.09 ± 0.465
3.311LysPro: 3.311 ± 0.381
2.337LysGln: 2.337 ± 0.435
4.155LysArg: 4.155 ± 0.642
3.636LysSer: 3.636 ± 0.424
4.155LysThr: 4.155 ± 0.492
4.545LysVal: 4.545 ± 0.511
1.298LysTrp: 1.298 ± 0.316
2.207LysTyr: 2.207 ± 0.429
0.0LysXaa: 0.0 ± 0.0
Leu
7.401LeuAla: 7.401 ± 0.951
0.909LeuCys: 0.909 ± 0.257
3.701LeuAsp: 3.701 ± 0.54
4.09LeuGlu: 4.09 ± 0.614
2.532LeuPhe: 2.532 ± 0.398
3.311LeuGly: 3.311 ± 0.477
1.428LeuHis: 1.428 ± 0.305
4.999LeuIle: 4.999 ± 0.564
5.194LeuLys: 5.194 ± 0.587
4.739LeuLeu: 4.739 ± 0.463
2.142LeuMet: 2.142 ± 0.353
3.895LeuAsn: 3.895 ± 0.464
3.116LeuPro: 3.116 ± 0.545
2.078LeuGln: 2.078 ± 0.372
3.376LeuArg: 3.376 ± 0.457
4.285LeuSer: 4.285 ± 0.474
5.389LeuThr: 5.389 ± 0.609
3.895LeuVal: 3.895 ± 0.423
1.039LeuTrp: 1.039 ± 0.254
2.207LeuTyr: 2.207 ± 0.436
0.0LeuXaa: 0.0 ± 0.0
Met
2.792MetAla: 2.792 ± 0.442
0.39MetCys: 0.39 ± 0.136
1.688MetAsp: 1.688 ± 0.319
1.363MetGlu: 1.363 ± 0.284
1.688MetPhe: 1.688 ± 0.362
1.428MetGly: 1.428 ± 0.336
0.779MetHis: 0.779 ± 0.202
2.662MetIle: 2.662 ± 0.42
2.467MetLys: 2.467 ± 0.375
2.727MetLeu: 2.727 ± 0.498
1.104MetMet: 1.104 ± 0.349
1.623MetAsn: 1.623 ± 0.304
0.909MetPro: 0.909 ± 0.183
1.818MetGln: 1.818 ± 0.32
1.948MetArg: 1.948 ± 0.339
1.623MetSer: 1.623 ± 0.333
1.818MetThr: 1.818 ± 0.358
1.493MetVal: 1.493 ± 0.285
0.519MetTrp: 0.519 ± 0.183
0.649MetTyr: 0.649 ± 0.206
0.0MetXaa: 0.0 ± 0.0
Asn
4.09AsnAla: 4.09 ± 0.506
0.714AsnCys: 0.714 ± 0.246
3.441AsnAsp: 3.441 ± 0.457
3.83AsnGlu: 3.83 ± 0.546
1.428AsnPhe: 1.428 ± 0.315
5.064AsnGly: 5.064 ± 0.59
1.039AsnHis: 1.039 ± 0.332
3.441AsnIle: 3.441 ± 0.438
3.895AsnLys: 3.895 ± 0.475
3.246AsnLeu: 3.246 ± 0.438
0.974AsnMet: 0.974 ± 0.289
2.337AsnAsn: 2.337 ± 0.375
1.493AsnPro: 1.493 ± 0.348
1.818AsnGln: 1.818 ± 0.418
2.402AsnArg: 2.402 ± 0.378
3.051AsnSer: 3.051 ± 0.464
1.818AsnThr: 1.818 ± 0.295
4.155AsnVal: 4.155 ± 0.386
0.519AsnTrp: 0.519 ± 0.159
1.558AsnTyr: 1.558 ± 0.274
0.0AsnXaa: 0.0 ± 0.0
Pro
2.272ProAla: 2.272 ± 0.379
0.39ProCys: 0.39 ± 0.2
2.467ProAsp: 2.467 ± 0.423
3.246ProGlu: 3.246 ± 0.505
1.428ProPhe: 1.428 ± 0.324
2.727ProGly: 2.727 ± 0.405
0.39ProHis: 0.39 ± 0.174
2.078ProIle: 2.078 ± 0.41
1.558ProLys: 1.558 ± 0.294
1.558ProLeu: 1.558 ± 0.235
0.844ProMet: 0.844 ± 0.214
1.688ProAsn: 1.688 ± 0.324
1.234ProPro: 1.234 ± 0.324
1.363ProGln: 1.363 ± 0.359
1.298ProArg: 1.298 ± 0.336
1.234ProSer: 1.234 ± 0.272
1.169ProThr: 1.169 ± 0.179
2.857ProVal: 2.857 ± 0.344
0.26ProTrp: 0.26 ± 0.145
1.234ProTyr: 1.234 ± 0.232
0.0ProXaa: 0.0 ± 0.0
Gln
3.116GlnAla: 3.116 ± 0.465
0.454GlnCys: 0.454 ± 0.169
2.337GlnAsp: 2.337 ± 0.358
2.857GlnGlu: 2.857 ± 0.392
1.363GlnPhe: 1.363 ± 0.255
1.883GlnGly: 1.883 ± 0.342
0.325GlnHis: 0.325 ± 0.127
3.181GlnIle: 3.181 ± 0.503
2.727GlnLys: 2.727 ± 0.526
3.376GlnLeu: 3.376 ± 0.548
0.909GlnMet: 0.909 ± 0.217
1.558GlnAsn: 1.558 ± 0.382
1.298GlnPro: 1.298 ± 0.281
2.727GlnGln: 2.727 ± 0.574
2.402GlnArg: 2.402 ± 0.475
2.013GlnSer: 2.013 ± 0.427
1.948GlnThr: 1.948 ± 0.416
2.467GlnVal: 2.467 ± 0.422
0.649GlnTrp: 0.649 ± 0.203
1.363GlnTyr: 1.363 ± 0.249
0.0GlnXaa: 0.0 ± 0.0
Arg
4.09ArgAla: 4.09 ± 0.477
1.039ArgCys: 1.039 ± 0.314
2.532ArgAsp: 2.532 ± 0.356
3.895ArgGlu: 3.895 ± 0.468
2.207ArgPhe: 2.207 ± 0.358
3.051ArgGly: 3.051 ± 0.449
0.909ArgHis: 0.909 ± 0.249
4.415ArgIle: 4.415 ± 0.502
4.025ArgLys: 4.025 ± 0.506
2.857ArgLeu: 2.857 ± 0.431
1.558ArgMet: 1.558 ± 0.269
2.013ArgAsn: 2.013 ± 0.433
1.558ArgPro: 1.558 ± 0.347
1.818ArgGln: 1.818 ± 0.4
3.246ArgArg: 3.246 ± 0.597
2.272ArgSer: 2.272 ± 0.336
1.558ArgThr: 1.558 ± 0.297
4.609ArgVal: 4.609 ± 0.462
0.519ArgTrp: 0.519 ± 0.168
2.142ArgTyr: 2.142 ± 0.399
0.0ArgXaa: 0.0 ± 0.0
Ser
4.674SerAla: 4.674 ± 0.583
0.649SerCys: 0.649 ± 0.194
3.701SerAsp: 3.701 ± 0.527
4.545SerGlu: 4.545 ± 0.462
1.948SerPhe: 1.948 ± 0.248
5.453SerGly: 5.453 ± 0.581
0.974SerHis: 0.974 ± 0.304
4.22SerIle: 4.22 ± 0.503
3.571SerLys: 3.571 ± 0.493
3.441SerLeu: 3.441 ± 0.398
1.688SerMet: 1.688 ± 0.285
1.948SerAsn: 1.948 ± 0.322
1.623SerPro: 1.623 ± 0.385
2.207SerGln: 2.207 ± 0.427
2.207SerArg: 2.207 ± 0.457
2.207SerSer: 2.207 ± 0.473
2.857SerThr: 2.857 ± 0.384
3.96SerVal: 3.96 ± 0.453
1.169SerTrp: 1.169 ± 0.224
2.337SerTyr: 2.337 ± 0.44
0.0SerXaa: 0.0 ± 0.0
Thr
4.025ThrAla: 4.025 ± 0.65
0.454ThrCys: 0.454 ± 0.221
3.441ThrAsp: 3.441 ± 0.558
2.662ThrGlu: 2.662 ± 0.395
3.116ThrPhe: 3.116 ± 0.364
5.129ThrGly: 5.129 ± 0.53
0.844ThrHis: 0.844 ± 0.236
3.246ThrIle: 3.246 ± 0.406
3.636ThrLys: 3.636 ± 0.685
3.701ThrLeu: 3.701 ± 0.471
1.363ThrMet: 1.363 ± 0.248
2.078ThrAsn: 2.078 ± 0.395
2.467ThrPro: 2.467 ± 0.369
2.337ThrGln: 2.337 ± 0.501
2.078ThrArg: 2.078 ± 0.351
3.051ThrSer: 3.051 ± 0.485
1.948ThrThr: 1.948 ± 0.306
4.22ThrVal: 4.22 ± 0.678
0.974ThrTrp: 0.974 ± 0.222
2.013ThrTyr: 2.013 ± 0.399
0.0ThrXaa: 0.0 ± 0.0
Val
4.35ValAla: 4.35 ± 0.513
1.169ValCys: 1.169 ± 0.327
4.609ValAsp: 4.609 ± 0.5
5.389ValGlu: 5.389 ± 0.579
2.402ValPhe: 2.402 ± 0.41
4.22ValGly: 4.22 ± 0.394
1.169ValHis: 1.169 ± 0.241
5.389ValIle: 5.389 ± 0.531
5.583ValLys: 5.583 ± 0.649
4.934ValLeu: 4.934 ± 0.525
2.013ValMet: 2.013 ± 0.379
3.506ValAsn: 3.506 ± 0.435
1.883ValPro: 1.883 ± 0.349
1.948ValGln: 1.948 ± 0.291
3.441ValArg: 3.441 ± 0.474
3.96ValSer: 3.96 ± 0.527
4.22ValThr: 4.22 ± 0.48
4.025ValVal: 4.025 ± 0.495
1.363ValTrp: 1.363 ± 0.243
2.337ValTyr: 2.337 ± 0.375
0.0ValXaa: 0.0 ± 0.0
Trp
1.039TrpAla: 1.039 ± 0.238
0.325TrpCys: 0.325 ± 0.144
1.039TrpAsp: 1.039 ± 0.225
0.974TrpGlu: 0.974 ± 0.314
0.714TrpPhe: 0.714 ± 0.254
1.298TrpGly: 1.298 ± 0.304
0.649TrpHis: 0.649 ± 0.192
0.844TrpIle: 0.844 ± 0.239
0.909TrpLys: 0.909 ± 0.243
1.363TrpLeu: 1.363 ± 0.276
0.26TrpMet: 0.26 ± 0.153
0.844TrpAsn: 0.844 ± 0.202
0.26TrpPro: 0.26 ± 0.125
0.325TrpGln: 0.325 ± 0.124
1.298TrpArg: 1.298 ± 0.268
0.779TrpSer: 0.779 ± 0.308
0.974TrpThr: 0.974 ± 0.237
0.779TrpVal: 0.779 ± 0.187
0.13TrpTrp: 0.13 ± 0.099
0.39TrpTyr: 0.39 ± 0.159
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.857TyrAla: 2.857 ± 0.573
0.649TyrCys: 0.649 ± 0.206
2.597TyrAsp: 2.597 ± 0.336
2.597TyrGlu: 2.597 ± 0.37
1.558TyrPhe: 1.558 ± 0.349
2.922TyrGly: 2.922 ± 0.425
1.039TyrHis: 1.039 ± 0.24
2.272TyrIle: 2.272 ± 0.358
3.051TyrLys: 3.051 ± 0.453
2.142TyrLeu: 2.142 ± 0.314
0.714TyrMet: 0.714 ± 0.22
1.688TyrAsn: 1.688 ± 0.377
1.104TyrPro: 1.104 ± 0.246
2.013TyrGln: 2.013 ± 0.275
2.013TyrArg: 2.013 ± 0.447
2.402TyrSer: 2.402 ± 0.501
1.948TyrThr: 1.948 ± 0.252
2.662TyrVal: 2.662 ± 0.351
0.325TyrTrp: 0.325 ± 0.126
1.363TyrTyr: 1.363 ± 0.324
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 87 proteins (15404 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski