Huangpi Tick Virus 3

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; unclassified Rhabdoviridae

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5KK14|A0A0B5KK14_9RHAB Putative matrix protein OS=Huangpi Tick Virus 3 OX=1608049 GN=ORF3 PE=4 SV=1
MM1 pKa = 7.22QRR3 pKa = 11.84QLPTLPDD10 pKa = 3.52HH11 pKa = 6.61VVEE14 pKa = 4.35ALRR17 pKa = 11.84DD18 pKa = 3.55LSEE21 pKa = 4.02EE22 pKa = 3.9RR23 pKa = 11.84RR24 pKa = 11.84VRR26 pKa = 11.84DD27 pKa = 3.42PLGLEE32 pKa = 3.92DD33 pKa = 3.66HH34 pKa = 7.05LEE36 pKa = 3.96RR37 pKa = 11.84SAYY40 pKa = 10.24RR41 pKa = 11.84RR42 pKa = 11.84MSASPARR49 pKa = 11.84MEE51 pKa = 4.32TEE53 pKa = 4.23ADD55 pKa = 3.28QDD57 pKa = 4.05PAPSGTPKK65 pKa = 10.49KK66 pKa = 9.87PRR68 pKa = 11.84KK69 pKa = 8.24QTGQDD74 pKa = 3.13KK75 pKa = 9.62PHH77 pKa = 7.14AGEE80 pKa = 4.02GARR83 pKa = 11.84LLRR86 pKa = 11.84STSKK90 pKa = 10.22QRR92 pKa = 11.84PEE94 pKa = 4.18VIGKK98 pKa = 9.75RR99 pKa = 11.84SKK101 pKa = 10.42ATDD104 pKa = 3.15RR105 pKa = 11.84PQRR108 pKa = 11.84SNVSMKK114 pKa = 10.47QGANQKK120 pKa = 9.72GGEE123 pKa = 4.24VSVQTHH129 pKa = 5.42TTPDD133 pKa = 3.63PSDD136 pKa = 3.55EE137 pKa = 4.33PTSEE141 pKa = 3.89MKK143 pKa = 10.52EE144 pKa = 4.05PNIGAEE150 pKa = 4.14GVVVLTEE157 pKa = 4.1PVPDD161 pKa = 5.09DD162 pKa = 4.82VIDD165 pKa = 4.2DD166 pKa = 3.53QMEE169 pKa = 4.07IDD171 pKa = 4.11KK172 pKa = 10.66QDD174 pKa = 3.64PSTNPQEE181 pKa = 4.48TYY183 pKa = 10.55FVATTSIFDD192 pKa = 3.95PADD195 pKa = 3.44LSGEE199 pKa = 4.31SLTAPQTLALQKK211 pKa = 10.58CALCMEE217 pKa = 4.69NLLAHH222 pKa = 6.95GFAKK226 pKa = 10.59DD227 pKa = 3.13ISADD231 pKa = 3.38VSNQGIVLRR240 pKa = 11.84YY241 pKa = 7.9TRR243 pKa = 11.84LLPAFDD249 pKa = 4.42TPPGEE254 pKa = 4.43QTCTQPPATDD264 pKa = 3.6PASAHH269 pKa = 5.92QKK271 pKa = 9.78SQDD274 pKa = 3.45KK275 pKa = 10.56QGPPDD280 pKa = 4.04KK281 pKa = 9.78EE282 pKa = 4.14APPAPTSDD290 pKa = 3.91KK291 pKa = 11.04NLEE294 pKa = 4.25PPLDD298 pKa = 4.43DD299 pKa = 5.75DD300 pKa = 3.98EE301 pKa = 4.88QFARR305 pKa = 11.84QVYY308 pKa = 9.54RR309 pKa = 11.84ALQDD313 pKa = 3.15MTIEE317 pKa = 4.3VPSRR321 pKa = 11.84SPGQMLSVKK330 pKa = 10.03VSAVPLRR337 pKa = 11.84WSVVWDD343 pKa = 3.26YY344 pKa = 11.83LKK346 pKa = 11.15AHH348 pKa = 6.23NRR350 pKa = 11.84VNWFIDD356 pKa = 3.72SASDD360 pKa = 3.6PAGKK364 pKa = 9.68LAFCAWSCRR373 pKa = 11.84VPSLMNTISPVFIRR387 pKa = 11.84DD388 pKa = 3.84AIKK391 pKa = 10.42GAPVV395 pKa = 2.82

Molecular weight: 43.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5KTD9|A0A0B5KTD9_9RHAB GDP polyribonucleotidyltransferase OS=Huangpi Tick Virus 3 OX=1608049 GN=L PE=4 SV=1
MM1 pKa = 7.31RR2 pKa = 11.84TEE4 pKa = 4.47LCLSCLLLLIVGHH17 pKa = 6.6GLLQEE22 pKa = 4.15WSTRR26 pKa = 11.84PEE28 pKa = 4.25PPDD31 pKa = 3.72EE32 pKa = 4.47STMSDD37 pKa = 3.24PPRR40 pKa = 11.84PVKK43 pKa = 10.37ARR45 pKa = 11.84CPFRR49 pKa = 11.84LIKK52 pKa = 10.79DD53 pKa = 3.46PAITLLGKK61 pKa = 10.11FRR63 pKa = 11.84TVRR66 pKa = 11.84SSLPIDD72 pKa = 4.22PEE74 pKa = 4.8SITPGQWVSQRR85 pKa = 11.84VRR87 pKa = 11.84VTKK90 pKa = 7.2YY91 pKa = 7.39TCQWGYY97 pKa = 7.6PTIVHH102 pKa = 5.12VAVRR106 pKa = 11.84HH107 pKa = 4.8VVPSLKK113 pKa = 10.24DD114 pKa = 3.52LKK116 pKa = 11.0NLQQVNMDD124 pKa = 3.93GEE126 pKa = 4.59TQITSLPCPWYY137 pKa = 8.41PWSGTHH143 pKa = 6.56IMKK146 pKa = 10.15QEE148 pKa = 3.86SVIRR152 pKa = 11.84EE153 pKa = 3.97NKK155 pKa = 9.37SVRR158 pKa = 11.84FHH160 pKa = 6.86IGSSSYY166 pKa = 11.13FDD168 pKa = 4.03FDD170 pKa = 4.49FPLWTCKK177 pKa = 10.35LPPCVTTNPAGMWIPNGGYY196 pKa = 9.63PSQCSSWHH204 pKa = 5.83EE205 pKa = 3.98IEE207 pKa = 5.71LEE209 pKa = 3.93LLGVTRR215 pKa = 11.84ADD217 pKa = 3.34PEE219 pKa = 4.54TYY221 pKa = 9.85HH222 pKa = 6.15VVGMYY227 pKa = 9.94SYY229 pKa = 10.14PVPLRR234 pKa = 11.84KK235 pKa = 9.75SCTCTTCSRR244 pKa = 11.84NVIVLPTGDD253 pKa = 4.2LLEE256 pKa = 5.37IPLAPQISKK265 pKa = 10.26ILSSYY270 pKa = 8.53PRR272 pKa = 11.84CSISIHH278 pKa = 6.51RR279 pKa = 11.84KK280 pKa = 7.05TGLHH284 pKa = 6.33FDD286 pKa = 3.7PRR288 pKa = 11.84GLPSEE293 pKa = 4.66LLDD296 pKa = 3.45LEE298 pKa = 4.22NYY300 pKa = 8.41YY301 pKa = 10.8RR302 pKa = 11.84CTLTHH307 pKa = 6.18LTYY310 pKa = 10.44ILRR313 pKa = 11.84PPPPQLLIPLLQSDD327 pKa = 4.41SAFSLIKK334 pKa = 10.03YY335 pKa = 8.04QLNNGYY341 pKa = 10.06LSPSLSSVGVKK352 pKa = 10.56GGDD355 pKa = 3.45SQPTHH360 pKa = 5.7SQILTLHH367 pKa = 5.84IVGALLHH374 pKa = 6.11SHH376 pKa = 7.19ADD378 pKa = 3.35QNIFTRR384 pKa = 11.84YY385 pKa = 10.02DD386 pKa = 3.59YY387 pKa = 9.4NTNWSMIDD395 pKa = 3.62DD396 pKa = 5.58IIRR399 pKa = 11.84EE400 pKa = 3.94MDD402 pKa = 3.35QTVEE406 pKa = 4.36DD407 pKa = 4.44LTAQTDD413 pKa = 3.95PEE415 pKa = 4.51KK416 pKa = 10.08TQHH419 pKa = 6.37PLNRR423 pKa = 11.84QNEE426 pKa = 4.47PIFKK430 pKa = 9.88FEE432 pKa = 4.84HH433 pKa = 5.43IMYY436 pKa = 10.22LVMLISGLLITFSTFFVISKK456 pKa = 10.15KK457 pKa = 9.35EE458 pKa = 3.79QTSEE462 pKa = 3.92VLTGNNSSTSSTGKK476 pKa = 9.0RR477 pKa = 11.84RR478 pKa = 11.84KK479 pKa = 9.38VKK481 pKa = 9.34RR482 pKa = 11.84TPEE485 pKa = 3.78VWSNPSRR492 pKa = 11.84RR493 pKa = 11.84IKK495 pKa = 9.82WNCKK499 pKa = 6.45TSCSCTHH506 pKa = 7.14ASPTKK511 pKa = 10.15FMRR514 pKa = 11.84VPSRR518 pKa = 11.84SLASGKK524 pKa = 10.32SSSSS528 pKa = 3.18

Molecular weight: 59.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3874

272

2193

774.8

86.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.453 ± 0.976

1.936 ± 0.239

5.679 ± 0.563

5.395 ± 0.351

3.82 ± 0.66

5.627 ± 0.224

3.072 ± 0.296

4.75 ± 0.383

3.975 ± 0.458

10.119 ± 1.317

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.349 ± 0.153

3.149 ± 0.333

6.298 ± 1.189

3.975 ± 0.354

6.531 ± 0.213

8.854 ± 0.683

6.608 ± 0.473

6.557 ± 0.127

1.91 ± 0.144

2.839 ± 0.25
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski