Staphylococcus phage St 134

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Rountreeviridae; Rakietenvirinae; Andhravirus; Staphylococcus virus St134

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 21 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S6KVF3|A0A1S6KVF3_9CAUD Upper collar protein OS=Staphylococcus phage St 134 OX=1958922 PE=4 SV=1
MM1 pKa = 8.15AEE3 pKa = 3.77EE4 pKa = 3.79EE5 pKa = 4.46KK6 pKa = 10.46IIKK9 pKa = 9.53EE10 pKa = 4.07EE11 pKa = 4.29PEE13 pKa = 4.26TEE15 pKa = 4.27STEE18 pKa = 4.04QPAEE22 pKa = 4.35KK23 pKa = 9.73IEE25 pKa = 4.2SAEE28 pKa = 4.12DD29 pKa = 3.57VVTEE33 pKa = 4.07PEE35 pKa = 4.15KK36 pKa = 10.94EE37 pKa = 4.12VTEE40 pKa = 4.37EE41 pKa = 3.74KK42 pKa = 10.79SEE44 pKa = 4.29AFVQLEE50 pKa = 3.81QRR52 pKa = 11.84ISSLEE57 pKa = 3.61QRR59 pKa = 11.84LNNLEE64 pKa = 4.12SQPSQEE70 pKa = 4.04SSDD73 pKa = 3.69PNFEE77 pKa = 4.88DD78 pKa = 3.17KK79 pKa = 10.41TVPTEE84 pKa = 3.76VDD86 pKa = 3.68DD87 pKa = 4.35NQEE90 pKa = 3.67TDD92 pKa = 4.05GIEE95 pKa = 3.81SSEE98 pKa = 4.73EE99 pKa = 3.58IKK101 pKa = 11.14QMLNLL106 pKa = 3.95

Molecular weight: 12.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S6KVD3|A0A1S6KVD3_9CAUD DNA encapsidation protein OS=Staphylococcus phage St 134 OX=1958922 PE=4 SV=1
MM1 pKa = 7.12YY2 pKa = 10.26FVDD5 pKa = 5.2LSLKK9 pKa = 10.26IFVATRR15 pKa = 11.84SNSVLVSGWGLVKK28 pKa = 10.39YY29 pKa = 9.87QKK31 pKa = 10.67NIPP34 pKa = 3.6

Molecular weight: 3.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21

0

21

5858

34

763

279.0

32.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.165 ± 0.376

0.751 ± 0.2

7.05 ± 0.352

6.538 ± 0.585

5.155 ± 0.374

5.582 ± 0.85

2.168 ± 0.267

7.853 ± 0.465

7.989 ± 0.579

7.187 ± 0.5

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.612 ± 0.193

8.587 ± 0.439

2.544 ± 0.366

3.756 ± 0.249

3.67 ± 0.343

6.384 ± 0.364

6.18 ± 0.387

5.104 ± 0.379

0.819 ± 0.19

5.906 ± 0.33
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski