Corynebacterium phage Stiles

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Samwavirus; Corynebacterium virus Stiles

Average proteome isoelectric point is 5.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y6EMW4|A0A4Y6EMW4_9CAUD DNA polymerase I OS=Corynebacterium phage Stiles OX=2588504 GN=45 PE=4 SV=1
MM1 pKa = 7.88TDD3 pKa = 3.23PDD5 pKa = 4.4FPDD8 pKa = 3.66SYY10 pKa = 11.23KK11 pKa = 10.64LLHH14 pKa = 6.18GAPYY18 pKa = 10.72DD19 pKa = 4.53DD20 pKa = 6.43DD21 pKa = 5.09PGPWPYY27 pKa = 10.86LAVIAFAAIAAVAILVASYY46 pKa = 10.82LL47 pKa = 3.74

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y6EMU2|A0A4Y6EMU2_9CAUD Terminase large subunit OS=Corynebacterium phage Stiles OX=2588504 GN=2 PE=4 SV=1
MM1 pKa = 7.5NFPSAVVQRR10 pKa = 11.84FWAKK14 pKa = 10.31VDD16 pKa = 2.83IRR18 pKa = 11.84QPDD21 pKa = 3.86EE22 pKa = 3.78CWNWQAYY29 pKa = 8.21LASTGYY35 pKa = 10.64GQFGTGGRR43 pKa = 11.84IRR45 pKa = 11.84YY46 pKa = 6.47AHH48 pKa = 6.25RR49 pKa = 11.84VAFEE53 pKa = 4.01IGTGRR58 pKa = 11.84SPAGLVVRR66 pKa = 11.84HH67 pKa = 5.58TCDD70 pKa = 3.11NRR72 pKa = 11.84ACVNPAHH79 pKa = 6.92LLLGTHH85 pKa = 7.2ADD87 pKa = 3.48NVRR90 pKa = 11.84DD91 pKa = 3.52KK92 pKa = 11.09VARR95 pKa = 11.84NRR97 pKa = 11.84QLRR100 pKa = 11.84GEE102 pKa = 4.13QHH104 pKa = 6.1GGAVLTDD111 pKa = 3.44AAVIGMRR118 pKa = 11.84RR119 pKa = 11.84RR120 pKa = 11.84ARR122 pKa = 11.84AGEE125 pKa = 4.0PIIRR129 pKa = 11.84IAADD133 pKa = 3.02YY134 pKa = 9.76GVNYY138 pKa = 7.86RR139 pKa = 11.84TAVTAIRR146 pKa = 11.84GDD148 pKa = 3.16RR149 pKa = 11.84GMWNHH154 pKa = 4.59VTEE157 pKa = 4.64PPVRR161 pKa = 11.84PATRR165 pKa = 11.84SPRR168 pKa = 11.84KK169 pKa = 9.46EE170 pKa = 3.3VDD172 pKa = 2.85AAA174 pKa = 3.85

Molecular weight: 19.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

13401

47

1731

248.2

26.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.044 ± 0.611

0.582 ± 0.093

6.947 ± 0.375

6.388 ± 0.249

2.485 ± 0.191

8.708 ± 0.557

2.358 ± 0.245

4.724 ± 0.205

3.239 ± 0.149

7.887 ± 0.356

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.477 ± 0.118

2.268 ± 0.188

5.455 ± 0.366

3.47 ± 0.185

7.096 ± 0.346

4.813 ± 0.309

6.388 ± 0.249

7.186 ± 0.319

2.231 ± 0.168

2.254 ± 0.223
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski