Testudo hermanni ranavirus

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Pimascovirales; Iridoviridae; Alphairidovirinae; Ranavirus; Common midwife toad virus

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D3R3J1|A0A0D3R3J1_9VIRU Putative cytosine DNA methyltransferase OS=Testudo hermanni ranavirus OX=89464 GN=CH8/96_ORF20L PE=4 SV=1
MM1 pKa = 7.4KK2 pKa = 9.12QTCCLLFAGDD12 pKa = 4.02LRR14 pKa = 11.84FTGEE18 pKa = 4.16LFFTGDD24 pKa = 3.26LRR26 pKa = 11.84FTGEE30 pKa = 4.08LFFTEE35 pKa = 4.51DD36 pKa = 3.45LRR38 pKa = 11.84FTGEE42 pKa = 4.03LFFTGDD48 pKa = 3.26LRR50 pKa = 11.84FTGEE54 pKa = 4.16LFFTGDD60 pKa = 3.26LRR62 pKa = 11.84FTGEE66 pKa = 4.08LFFTEE71 pKa = 4.51DD72 pKa = 3.45LRR74 pKa = 11.84FTGEE78 pKa = 3.99LFFTEE83 pKa = 4.59DD84 pKa = 3.96LRR86 pKa = 11.84FTGDD90 pKa = 3.4FLIGEE95 pKa = 4.31LFFRR99 pKa = 11.84GDD101 pKa = 3.26LSTGGFRR108 pKa = 11.84FTRR111 pKa = 11.84DD112 pKa = 3.22FLFLTGAEE120 pKa = 4.16ALTTGADD127 pKa = 3.52SIAPRR132 pKa = 11.84EE133 pKa = 4.13GPNIIGEE140 pKa = 4.21APCLGEE146 pKa = 4.51QPTEE150 pKa = 4.08HH151 pKa = 7.22PEE153 pKa = 3.79LRR155 pKa = 11.84TLYY158 pKa = 10.35ISGLL162 pKa = 3.7

Molecular weight: 18.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D3R314|A0A0D3R314_9VIRU Putative deoxynucleoside kinase OS=Testudo hermanni ranavirus OX=89464 GN=CH8/96_ORF18L PE=4 SV=1
MM1 pKa = 7.49QVFLDD6 pKa = 3.67LDD8 pKa = 3.57EE9 pKa = 4.67TLIHH13 pKa = 6.72SIPVSRR19 pKa = 11.84LGWTKK24 pKa = 10.77SKK26 pKa = 10.11PYY28 pKa = 9.05PVKK31 pKa = 10.24PFTVQDD37 pKa = 3.29VGTPLSVMMGSSKK50 pKa = 10.5VVNDD54 pKa = 3.15GRR56 pKa = 11.84KK57 pKa = 9.32RR58 pKa = 11.84LATRR62 pKa = 11.84LSLFKK67 pKa = 10.37RR68 pKa = 11.84TVLTDD73 pKa = 5.34HH74 pKa = 6.53IMCWRR79 pKa = 11.84PTLRR83 pKa = 11.84TFLNGLFASGYY94 pKa = 9.6KK95 pKa = 9.87INVWTAASKK104 pKa = 10.16PYY106 pKa = 10.58ALEE109 pKa = 3.88VVKK112 pKa = 10.78ALNLKK117 pKa = 9.9SYY119 pKa = 11.06GMGLLVTAQDD129 pKa = 3.96YY130 pKa = 9.2PKK132 pKa = 11.08GSVKK136 pKa = 10.19RR137 pKa = 11.84LKK139 pKa = 10.9YY140 pKa = 9.88LTGLDD145 pKa = 3.37AVKK148 pKa = 10.43IPLSNTAIVDD158 pKa = 3.36DD159 pKa = 4.17RR160 pKa = 11.84EE161 pKa = 4.24EE162 pKa = 4.07VKK164 pKa = 10.52RR165 pKa = 11.84AQPTRR170 pKa = 11.84AVHH173 pKa = 6.36IKK175 pKa = 10.27PFTASRR181 pKa = 11.84ANTVCSEE188 pKa = 3.79SDD190 pKa = 3.05EE191 pKa = 4.46LKK193 pKa = 10.78RR194 pKa = 11.84VTASLAIIAGRR205 pKa = 11.84SRR207 pKa = 11.84SRR209 pKa = 11.84RR210 pKa = 11.84RR211 pKa = 3.14

Molecular weight: 23.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

26980

90

1294

359.7

39.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.033 ± 0.271

2.12 ± 0.142

6.142 ± 0.159

5.808 ± 0.225

3.466 ± 0.163

7.257 ± 0.239

1.998 ± 0.127

3.273 ± 0.15

5.578 ± 0.277

8.006 ± 0.254

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.032 ± 0.138

2.557 ± 0.144

6.193 ± 0.308

2.761 ± 0.208

6.898 ± 0.299

7.142 ± 0.2

5.875 ± 0.185

8.388 ± 0.236

1.486 ± 0.111

2.987 ± 0.132
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski