Klebsiella phage KN3-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Przondovirus; Klebsiella virus KN3-1

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q9WWY9|A0A3Q9WWY9_9CAUD DNA-directed RNA polymerase OS=Klebsiella phage KN3-1 OX=2282630 PE=3 SV=1
MM1 pKa = 7.69AGEE4 pKa = 4.42SNADD8 pKa = 3.27VYY10 pKa = 11.74ASFGVNSAVLTGSTPEE26 pKa = 3.81EE27 pKa = 4.08HH28 pKa = 6.29QEE30 pKa = 3.72NMLALDD36 pKa = 3.66VAARR40 pKa = 11.84DD41 pKa = 3.53GDD43 pKa = 4.19DD44 pKa = 5.06AIEE47 pKa = 4.63LNTNSDD53 pKa = 3.81DD54 pKa = 4.22PYY56 pKa = 11.43GSDD59 pKa = 3.19VDD61 pKa = 3.98PFGEE65 pKa = 4.26PEE67 pKa = 3.93EE68 pKa = 5.05GRR70 pKa = 11.84MQVRR74 pKa = 11.84ISADD78 pKa = 3.11GDD80 pKa = 3.73DD81 pKa = 4.28PEE83 pKa = 4.79EE84 pKa = 3.96EE85 pKa = 4.18VVEE88 pKa = 4.41EE89 pKa = 4.5EE90 pKa = 4.22EE91 pKa = 4.24QQGDD95 pKa = 3.71EE96 pKa = 4.16EE97 pKa = 4.7GQLEE101 pKa = 4.4EE102 pKa = 4.2VTEE105 pKa = 4.18EE106 pKa = 4.44GEE108 pKa = 4.11PEE110 pKa = 3.89EE111 pKa = 4.3FKK113 pKa = 11.04PIGEE117 pKa = 4.36TPADD121 pKa = 3.26ISEE124 pKa = 4.49ASQQLEE130 pKa = 3.83EE131 pKa = 4.93HH132 pKa = 6.15EE133 pKa = 5.18AGFNDD138 pKa = 3.81MVATAIEE145 pKa = 4.42RR146 pKa = 11.84GLSQDD151 pKa = 2.65AVTRR155 pKa = 11.84IQQEE159 pKa = 4.18YY160 pKa = 9.09QNEE163 pKa = 3.96DD164 pKa = 3.44RR165 pKa = 11.84LSDD168 pKa = 3.51EE169 pKa = 4.57SYY171 pKa = 11.21KK172 pKa = 10.72EE173 pKa = 3.78LAEE176 pKa = 4.32AGYY179 pKa = 11.04SKK181 pKa = 11.26AFVDD185 pKa = 4.66AYY187 pKa = 10.18IRR189 pKa = 11.84GQEE192 pKa = 4.01ALVNQYY198 pKa = 8.39VEE200 pKa = 4.13KK201 pKa = 11.31VMDD204 pKa = 3.92FVGGRR209 pKa = 11.84EE210 pKa = 3.86RR211 pKa = 11.84FQQVYY216 pKa = 7.32THH218 pKa = 6.02MQTNNPEE225 pKa = 3.88GAEE228 pKa = 3.93ALIKK232 pKa = 10.63AFEE235 pKa = 4.23SRR237 pKa = 11.84DD238 pKa = 3.48VATMKK243 pKa = 10.36TILNLAGQSRR253 pKa = 11.84DD254 pKa = 3.16KK255 pKa = 11.32TFGKK259 pKa = 10.22KK260 pKa = 9.91AEE262 pKa = 3.85RR263 pKa = 11.84SIAKK267 pKa = 9.62RR268 pKa = 11.84ATPAKK273 pKa = 9.25PVARR277 pKa = 11.84KK278 pKa = 10.1AEE280 pKa = 4.23GFEE283 pKa = 4.58SQAEE287 pKa = 4.38MIKK290 pKa = 10.76AMSDD294 pKa = 2.8PRR296 pKa = 11.84YY297 pKa = 9.04RR298 pKa = 11.84TDD300 pKa = 2.52SKK302 pKa = 11.03YY303 pKa = 10.48RR304 pKa = 11.84RR305 pKa = 11.84EE306 pKa = 4.17VEE308 pKa = 4.0QKK310 pKa = 11.01VIDD313 pKa = 3.98SKK315 pKa = 11.46FF316 pKa = 3.06

Molecular weight: 35.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q9WSD5|A0A3Q9WSD5_9CAUD Endolysin OS=Klebsiella phage KN3-1 OX=2282630 PE=3 SV=1
MM1 pKa = 7.9PYY3 pKa = 10.3SSSNHH8 pKa = 5.51LSYY11 pKa = 11.18VPSSMSFCLNSRR23 pKa = 11.84GCCRR27 pKa = 11.84KK28 pKa = 10.02RR29 pKa = 11.84IPLAIPKK36 pKa = 8.31TSSMVSSTDD45 pKa = 2.99SSSIAMPTRR54 pKa = 11.84SPCPLANPRR63 pKa = 11.84ACWRR67 pKa = 11.84TNVDD71 pKa = 4.43LPIPDD76 pKa = 4.8PEE78 pKa = 4.33VTMMTSPPRR87 pKa = 11.84APKK90 pKa = 10.02VRR92 pKa = 11.84SFSPGHH98 pKa = 5.51PEE100 pKa = 3.28KK101 pKa = 10.48RR102 pKa = 11.84YY103 pKa = 7.6PTLCSLVMASRR114 pKa = 11.84TLSFMDD120 pKa = 4.14IAPITPSGTQGAAFHH135 pKa = 6.44IWSSISLPLPLSKK148 pKa = 10.63HH149 pKa = 5.34SLASFSVSTATWTLPGGRR167 pKa = 11.84TT168 pKa = 3.32

Molecular weight: 18.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24

0

24

11193

167

1321

466.4

51.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.702 ± 0.624

1.126 ± 0.214

6.352 ± 0.219

6.522 ± 0.511

3.565 ± 0.207

8.184 ± 0.414

2.073 ± 0.275

4.664 ± 0.18

5.754 ± 0.45

8.684 ± 0.653

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.51 ± 0.197

4.181 ± 0.229

3.877 ± 0.263

4.494 ± 0.332

5.557 ± 0.268

6.057 ± 0.364

5.87 ± 0.332

7.281 ± 0.508

1.242 ± 0.18

3.306 ± 0.275
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski