Salmonella phage SS1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Jerseyvirus; unclassified Jerseyvirus

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C0CDY2|A0A5C0CDY2_9CAUD Oxidoreductase OS=Salmonella phage SS1 OX=2592214 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.45SKK4 pKa = 10.9VLIVTIEE11 pKa = 4.18TAWVGASEE19 pKa = 4.31VVEE22 pKa = 4.17IEE24 pKa = 3.98LDD26 pKa = 3.51GSEE29 pKa = 4.27TEE31 pKa = 4.8EE32 pKa = 4.33EE33 pKa = 4.43LEE35 pKa = 4.3DD36 pKa = 3.51MARR39 pKa = 11.84EE40 pKa = 3.99EE41 pKa = 4.15FGNYY45 pKa = 8.92CNYY48 pKa = 8.92GWRR51 pKa = 11.84VEE53 pKa = 4.24EE54 pKa = 4.19

Molecular weight: 6.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C0CDM1|A0A5C0CDM1_9CAUD Uncharacterized protein OS=Salmonella phage SS1 OX=2592214 PE=4 SV=1
MM1 pKa = 7.49AGVVVAVLYY10 pKa = 11.0NFTGGVKK17 pKa = 10.22HH18 pKa = 6.79LISSHH23 pKa = 5.02KK24 pKa = 9.99VIRR27 pKa = 11.84DD28 pKa = 3.58GLHH31 pKa = 5.94RR32 pKa = 11.84PADD35 pKa = 3.26IVAALVRR42 pKa = 11.84TRR44 pKa = 11.84LVVAVSKK51 pKa = 9.32TVVWGICLRR60 pKa = 11.84VRR62 pKa = 11.84KK63 pKa = 9.9VRR65 pKa = 11.84GRR67 pKa = 11.84TFHH70 pKa = 6.68GRR72 pKa = 11.84RR73 pKa = 11.84QEE75 pKa = 3.59LRR77 pKa = 11.84QRR79 pKa = 11.84FKK81 pKa = 11.63NNAKK85 pKa = 9.91NLHH88 pKa = 6.55DD89 pKa = 4.88AFNSATTSGFHH100 pKa = 5.59NVEE103 pKa = 3.72SRR105 pKa = 11.84VFFQCFEE112 pKa = 4.08VFCYY116 pKa = 10.53VVIFSNFVDD125 pKa = 4.09KK126 pKa = 10.87NAKK129 pKa = 9.76RR130 pKa = 11.84NPIRR134 pKa = 11.84NAGNGEE140 pKa = 4.43VNWVIAITFNVKK152 pKa = 10.09CAVFVTGIVDD162 pKa = 3.39YY163 pKa = 11.35

Molecular weight: 18.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

44

0

44

9837

29

777

223.6

24.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.942 ± 0.767

0.813 ± 0.164

6.272 ± 0.228

6.801 ± 0.419

3.843 ± 0.2

7.767 ± 0.362

1.352 ± 0.184

4.981 ± 0.19

5.805 ± 0.338

7.97 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.47 ± 0.162

4.432 ± 0.247

3.629 ± 0.265

3.893 ± 0.262

5.652 ± 0.282

6.018 ± 0.338

6.364 ± 0.341

7.492 ± 0.412

1.403 ± 0.153

3.101 ± 0.156
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski