Pteropus alecto (Black flying fox)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Laurasiatheria; Chiroptera; Megachiroptera;

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19521 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L5KQ53|L5KQ53_PTEAL Uncharacterized protein OS=Pteropus alecto OX=9402 GN=PAL_GLEAN10001291 PE=4 SV=1
MM1 pKa = 8.24DD2 pKa = 4.75FQQLADD8 pKa = 4.22VAEE11 pKa = 4.85KK12 pKa = 9.68WCSHH16 pKa = 5.29TPFEE20 pKa = 5.61LIATEE25 pKa = 3.96EE26 pKa = 4.32TEE28 pKa = 4.33RR29 pKa = 11.84RR30 pKa = 11.84MDD32 pKa = 4.29FYY34 pKa = 11.47ADD36 pKa = 3.74PGVSFYY42 pKa = 10.98VLCPDD47 pKa = 4.64NGCGDD52 pKa = 3.96NFHH55 pKa = 6.3VWSEE59 pKa = 4.48SEE61 pKa = 3.97DD62 pKa = 3.63CLPFLQLAQDD72 pKa = 4.45YY73 pKa = 10.71ISSCGKK79 pKa = 8.66KK80 pKa = 8.01TLQEE84 pKa = 3.92VLEE87 pKa = 4.35KK88 pKa = 10.64VFKK91 pKa = 10.63SFRR94 pKa = 11.84PLLGLPDD101 pKa = 4.91ADD103 pKa = 4.57DD104 pKa = 4.81DD105 pKa = 4.54ALEE108 pKa = 4.66EE109 pKa = 4.06YY110 pKa = 10.6SADD113 pKa = 3.71VEE115 pKa = 4.38EE116 pKa = 5.09EE117 pKa = 4.2EE118 pKa = 5.29PEE120 pKa = 4.13ADD122 pKa = 3.66HH123 pKa = 6.65PQMGVSQQQ131 pKa = 3.0

Molecular weight: 14.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L5KK91|L5KK91_PTEAL CDP-diacylglycerol--inositol 3-phosphatidyltransferase OS=Pteropus alecto OX=9402 GN=PAL_GLEAN10011874 PE=3 SV=1
MM1 pKa = 7.47PHH3 pKa = 5.92QPRR6 pKa = 11.84HH7 pKa = 5.17RR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84ARR12 pKa = 11.84SSHH15 pKa = 5.15SQAGHH20 pKa = 6.02SGTRR24 pKa = 11.84SPPAPRR30 pKa = 11.84SPVPPRR36 pKa = 11.84SPVPPRR42 pKa = 11.84SPVPPRR48 pKa = 11.84SPVPPRR54 pKa = 11.84SPVPPRR60 pKa = 11.84SPVPPRR66 pKa = 11.84SPVPPRR72 pKa = 11.84SPVPPRR78 pKa = 11.84SPVPPRR84 pKa = 11.84SPVPPRR90 pKa = 11.84SPVPPRR96 pKa = 11.84SPVPPRR102 pKa = 11.84SPVPPRR108 pKa = 11.84SPVPPRR114 pKa = 11.84SPVPPRR120 pKa = 11.84SPVPPRR126 pKa = 11.84SPVPPRR132 pKa = 11.84SPVPPRR138 pKa = 11.84SPVPPRR144 pKa = 11.84SPVPPRR150 pKa = 11.84SPVPPRR156 pKa = 11.84SPVPPRR162 pKa = 11.84SPVPPRR168 pKa = 11.84SPVPPRR174 pKa = 11.84SPVPPRR180 pKa = 11.84SPVPPRR186 pKa = 11.84SPVPPRR192 pKa = 11.84SPVPPRR198 pKa = 11.84SPVPPRR204 pKa = 11.84SPVPPRR210 pKa = 11.84SPVPPRR216 pKa = 11.84SPVPPRR222 pKa = 11.84SPVPPRR228 pKa = 11.84SPVPPRR234 pKa = 11.84SPVPPRR240 pKa = 11.84SPVPPRR246 pKa = 11.84SPVPPRR252 pKa = 11.84SPVPPRR258 pKa = 11.84SPVPPRR264 pKa = 11.84SPVPPRR270 pKa = 11.84SPVPPRR276 pKa = 11.84SPVPPRR282 pKa = 11.84SPVPPRR288 pKa = 11.84SPVPPRR294 pKa = 11.84SPVPPRR300 pKa = 11.84SPVPPRR306 pKa = 11.84SPVPPRR312 pKa = 11.84SPVPPRR318 pKa = 11.84SPVPPRR324 pKa = 11.84SPVPPRR330 pKa = 11.84SPVPPRR336 pKa = 11.84SPVPPRR342 pKa = 11.84SPVPPRR348 pKa = 11.84SPVPPRR354 pKa = 11.84SPVPPRR360 pKa = 11.84SPVPPRR366 pKa = 11.84SPVPPRR372 pKa = 11.84SPVPPRR378 pKa = 11.84SPVPPRR384 pKa = 11.84SPVPPRR390 pKa = 11.84SPVPPRR396 pKa = 11.84SPVPPRR402 pKa = 11.84SPVPPRR408 pKa = 11.84SPVPPRR414 pKa = 11.84SPVPPRR420 pKa = 11.84SPVPPRR426 pKa = 11.84SPVPPRR432 pKa = 11.84SPVPPRR438 pKa = 11.84SPVPPRR444 pKa = 11.84SPVPPRR450 pKa = 11.84SPVPPRR456 pKa = 11.84SPVPPRR462 pKa = 11.84SPVPPRR468 pKa = 11.84SPVPPRR474 pKa = 11.84SPVPPRR480 pKa = 11.84SPVPPRR486 pKa = 11.84SPVPPRR492 pKa = 11.84SPVPPRR498 pKa = 11.84SPVPPRR504 pKa = 11.84SPVPPRR510 pKa = 11.84SPVPPRR516 pKa = 11.84SPVPPRR522 pKa = 11.84SPVPPRR528 pKa = 11.84SPVPPRR534 pKa = 11.84SPVPPRR540 pKa = 11.84SPVPPRR546 pKa = 11.84SPVPPRR552 pKa = 11.84SPVPPRR558 pKa = 11.84SPVPPRR564 pKa = 11.84SPVPPRR570 pKa = 11.84SPVPPRR576 pKa = 11.84SPVPPRR582 pKa = 11.84SPVPPRR588 pKa = 11.84SPVPPRR594 pKa = 11.84SPVPPRR600 pKa = 11.84SPVPPRR606 pKa = 11.84SPVPPRR612 pKa = 11.84SPVPPRR618 pKa = 11.84SPVPPRR624 pKa = 11.84SPVPPRR630 pKa = 11.84SPVPPRR636 pKa = 11.84SPVPPRR642 pKa = 11.84SPVPPRR648 pKa = 11.84SPVPPRR654 pKa = 11.84SPVPPRR660 pKa = 11.84SPVPPRR666 pKa = 11.84SPVPPRR672 pKa = 11.84SPVPPRR678 pKa = 11.84SPVPPRR684 pKa = 11.84SPVPPRR690 pKa = 11.84SPVPPRR696 pKa = 11.84SPVPPRR702 pKa = 11.84SPVPPRR708 pKa = 11.84SPVPPRR714 pKa = 11.84SPVPPRR720 pKa = 11.84SPVPPRR726 pKa = 11.84SPVPPRR732 pKa = 11.84SPVPPRR738 pKa = 11.84SPVPPRR744 pKa = 11.84SPVPPRR750 pKa = 11.84SPVPPRR756 pKa = 11.84SPVPPRR762 pKa = 11.84SPVPPRR768 pKa = 11.84SPVPPRR774 pKa = 11.84SPVPPRR780 pKa = 11.84SPVPPRR786 pKa = 11.84SPVPPRR792 pKa = 11.84SPVPPRR798 pKa = 11.84SPVPPRR804 pKa = 11.84SPVPPRR810 pKa = 11.84SPVPPRR816 pKa = 11.84SPVPPRR822 pKa = 11.84SPVPPRR828 pKa = 11.84SPVPPRR834 pKa = 11.84SPVPPRR840 pKa = 11.84SPVPPRR846 pKa = 11.84SPVPPRR852 pKa = 11.84SPVPPRR858 pKa = 11.84SPVPPRR864 pKa = 11.84SPVPPRR870 pKa = 11.84SPVPPRR876 pKa = 11.84SPVPPRR882 pKa = 11.84SPVPPRR888 pKa = 11.84SPVPPRR894 pKa = 11.84SPVPPRR900 pKa = 11.84SPVPPRR906 pKa = 11.84SPVPPRR912 pKa = 11.84SPVPPRR918 pKa = 11.84SPVPPRR924 pKa = 11.84SPVPPRR930 pKa = 11.84SPVPPRR936 pKa = 11.84SPVPPRR942 pKa = 11.84SPVPPRR948 pKa = 11.84SPVPPRR954 pKa = 11.84SPVPPRR960 pKa = 11.84SPVPPRR966 pKa = 11.84SPVPPRR972 pKa = 11.84SPVPPRR978 pKa = 11.84SPPAPRR984 pKa = 11.84SPPPHH989 pKa = 6.94WPTLGLLSSPVSLQGLCAEE1008 pKa = 4.44PSEE1011 pKa = 4.78VATSHH1016 pKa = 6.85LPPPSLEE1023 pKa = 3.92WQGVVGTPFSLRR1035 pKa = 11.84QTHH1038 pKa = 6.15YY1039 pKa = 10.16EE1040 pKa = 3.96LQALSSPSEE1049 pKa = 4.02LCDD1052 pKa = 3.54SGEE1055 pKa = 4.18SLNLSAPAASAVKK1068 pKa = 10.35

Molecular weight: 112.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19521

0

19521

9971383

28

34674

510.8

56.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.103 ± 0.02

2.213 ± 0.013

4.805 ± 0.012

7.05 ± 0.023

3.624 ± 0.014

6.536 ± 0.023

2.597 ± 0.009

4.28 ± 0.016

5.618 ± 0.021

10.033 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.131 ± 0.009

3.517 ± 0.013

6.285 ± 0.027

4.726 ± 0.019

5.755 ± 0.017

8.273 ± 0.023

5.271 ± 0.023

6.106 ± 0.018

1.215 ± 0.006

2.608 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski