Frondihabitans sp. PAMC 28766

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Frondihabitans; unclassified Frondihabitans

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4103 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126YWF7|A0A126YWF7_9MICO D-inositol 3-phosphate glycosyltransferase OS=Frondihabitans sp. PAMC 28766 OX=1795630 GN=AX769_02265 PE=4 SV=1
MM1 pKa = 7.5APAVTAQAAEE11 pKa = 4.04ATSYY15 pKa = 9.62GTFALAGGARR25 pKa = 11.84AYY27 pKa = 9.26TGTMLLPGGFPATTFTSTSRR47 pKa = 11.84QVTVISGASTWQAASTPVGAVYY69 pKa = 10.34GSSKK73 pKa = 10.24GMPYY77 pKa = 10.72LNQRR81 pKa = 11.84PMADD85 pKa = 3.25NAASPAVTTYY95 pKa = 11.26SFATPSPASGWSFVLGDD112 pKa = 4.09IDD114 pKa = 5.36ADD116 pKa = 3.73QATVSATDD124 pKa = 3.49ANGAAVPIADD134 pKa = 4.59LGFQGVYY141 pKa = 10.1NYY143 pKa = 10.04CHH145 pKa = 5.01QTGGPSCDD153 pKa = 3.39SANVGDD159 pKa = 4.26VPTWDD164 pKa = 4.05PATGTLIGNTQASDD178 pKa = 3.62TEE180 pKa = 4.74GASGWFSPSVPLKK193 pKa = 9.78TLTITYY199 pKa = 6.84QQRR202 pKa = 11.84SGLPVYY208 pKa = 7.23QTWFATKK215 pKa = 8.87TYY217 pKa = 9.62AASGVVTVDD226 pKa = 3.22GKK228 pKa = 10.13PYY230 pKa = 10.65GGAKK234 pKa = 10.19VSITDD239 pKa = 3.35ASGATVATVTAGGDD253 pKa = 4.06GTWSVPGLVAAAGYY267 pKa = 9.02HH268 pKa = 5.1VAVATPPGAQDD279 pKa = 3.38ATPLAFDD286 pKa = 3.91TTAADD291 pKa = 3.56ATALDD296 pKa = 3.99VDD298 pKa = 4.76FTIPPVTVTGTITDD312 pKa = 3.45SGGQPAADD320 pKa = 3.83EE321 pKa = 5.2PIVITRR327 pKa = 11.84DD328 pKa = 3.15GDD330 pKa = 3.58DD331 pKa = 3.83APAVTTTTDD340 pKa = 2.93SAGGFTADD348 pKa = 4.86LLPSQDD354 pKa = 3.1YY355 pKa = 11.29SLVVDD360 pKa = 4.91GATDD364 pKa = 3.25QAIAFTTPTTSGALAPLAQPAAPVTTPPVTPPVTPPVTSTPPATGTAPAAAGDD417 pKa = 4.15AGDD420 pKa = 3.8EE421 pKa = 4.01LAYY424 pKa = 9.91TGSQPAWPALAGGALVLGGLVITLGSARR452 pKa = 11.84RR453 pKa = 11.84RR454 pKa = 11.84RR455 pKa = 11.84SPAEE459 pKa = 3.63RR460 pKa = 4.22

Molecular weight: 45.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126YWX1|A0A126YWX1_9MICO Cell division protein CrgA OS=Frondihabitans sp. PAMC 28766 OX=1795630 GN=crgA PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 9.59AKK15 pKa = 9.91KK16 pKa = 8.54HH17 pKa = 4.37GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.68GRR40 pKa = 11.84TEE42 pKa = 4.13LSAA45 pKa = 4.86

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4103

0

4103

1237931

28

3005

301.7

32.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.047 ± 0.048

0.492 ± 0.009

6.162 ± 0.039

5.008 ± 0.044

3.223 ± 0.024

8.96 ± 0.036

2.039 ± 0.019

4.499 ± 0.029

2.253 ± 0.03

9.874 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.705 ± 0.016

2.092 ± 0.02

5.314 ± 0.028

2.884 ± 0.022

6.792 ± 0.048

6.507 ± 0.04

6.75 ± 0.058

8.949 ± 0.043

1.432 ± 0.016

2.02 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski