Salmonella phage St161

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Jerseyvirus; unclassified Jerseyvirus

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A291AX45|A0A291AX45_9CAUD Uncharacterized protein OS=Salmonella phage St161 OX=2024311 GN=St161_gp46 PE=4 SV=1
MM1 pKa = 6.5VARR4 pKa = 11.84YY5 pKa = 9.06RR6 pKa = 11.84FNYY9 pKa = 7.46VQEE12 pKa = 4.15MFEE15 pKa = 4.17TDD17 pKa = 3.69AGEE20 pKa = 4.25QYY22 pKa = 11.04CEE24 pKa = 4.14TEE26 pKa = 3.49RR27 pKa = 11.84DD28 pKa = 3.19EE29 pKa = 4.15WVKK32 pKa = 11.35YY33 pKa = 10.22EE34 pKa = 5.22DD35 pKa = 3.74YY36 pKa = 11.67AEE38 pKa = 4.29LKK40 pKa = 10.48EE41 pKa = 4.67LLDD44 pKa = 3.9ALAYY48 pKa = 10.45SVLNYY53 pKa = 9.63PLSPDD58 pKa = 3.48TEE60 pKa = 4.14EE61 pKa = 3.98LAKK64 pKa = 10.75GVLGDD69 pKa = 3.66

Molecular weight: 8.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A291AX81|A0A291AX81_9CAUD Uncharacterized protein OS=Salmonella phage St161 OX=2024311 GN=St161_gp45 PE=4 SV=1
MM1 pKa = 6.88YY2 pKa = 10.02RR3 pKa = 11.84EE4 pKa = 4.16KK5 pKa = 10.9GVAAVRR11 pKa = 11.84TPSGIRR17 pKa = 11.84FMGTRR22 pKa = 11.84NITRR26 pKa = 11.84QQLATLNRR34 pKa = 11.84IPQEE38 pKa = 3.95EE39 pKa = 4.56LEE41 pKa = 5.6AALKK45 pKa = 7.98WQKK48 pKa = 10.94AA49 pKa = 3.54

Molecular weight: 5.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

47

0

47

8991

46

796

191.3

21.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.577 ± 0.795

0.812 ± 0.11

5.973 ± 0.283

6.073 ± 0.396

3.492 ± 0.219

7.786 ± 0.312

1.524 ± 0.245

5.405 ± 0.216

5.428 ± 0.351

7.385 ± 0.293

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.436 ± 0.186

4.838 ± 0.296

3.77 ± 0.261

4.471 ± 0.515

5.105 ± 0.276

5.795 ± 0.301

6.718 ± 0.453

7.363 ± 0.395

1.401 ± 0.173

3.648 ± 0.239
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski