Mycobacterium phage Muddy

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Mapvirus; Mycobacterium virus Muddy

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5YB07|S5YB07_9CAUD Tail assembly chaperone OS=Mycobacterium phage Muddy OX=1340829 GN=17 PE=4 SV=1
MM1 pKa = 7.37SVPDD5 pKa = 3.53VFLPLLYY12 pKa = 10.74SVDD15 pKa = 3.21HH16 pKa = 6.34SAYY19 pKa = 10.55DD20 pKa = 3.5ATATNDD26 pKa = 3.79LGDD29 pKa = 5.93PIPDD33 pKa = 2.97WQEE36 pKa = 3.36PVEE39 pKa = 4.28RR40 pKa = 11.84DD41 pKa = 3.47VYY43 pKa = 11.21GWGPPQQEE51 pKa = 4.27TPKK54 pKa = 10.32EE55 pKa = 4.31VIVGDD60 pKa = 3.38DD61 pKa = 4.09RR62 pKa = 11.84IRR64 pKa = 11.84VEE66 pKa = 4.6LEE68 pKa = 3.72LMVPPGWQSKK78 pKa = 10.0HH79 pKa = 5.8RR80 pKa = 11.84DD81 pKa = 3.67KK82 pKa = 11.4FDD84 pKa = 3.44LGYY87 pKa = 11.07GDD89 pKa = 5.21DD90 pKa = 3.51VVYY93 pKa = 10.68YY94 pKa = 9.46QVGPVEE100 pKa = 5.25DD101 pKa = 4.63YY102 pKa = 10.75EE103 pKa = 4.57HH104 pKa = 7.02NPFGWNPGSVVNLVSIKK121 pKa = 10.76GVLL124 pKa = 3.25

Molecular weight: 13.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5YN75|S5YN75_9CAUD Uncharacterized protein OS=Mycobacterium phage Muddy OX=1340829 GN=70 PE=4 SV=1
MM1 pKa = 7.23TPIDD5 pKa = 3.9RR6 pKa = 11.84ALLSITKK13 pKa = 9.08TFLAVHH19 pKa = 5.74QLMPTRR25 pKa = 11.84GEE27 pKa = 3.86ALYY30 pKa = 10.68AALNKK35 pKa = 9.78PWEE38 pKa = 4.22KK39 pKa = 10.92QNWLALRR46 pKa = 11.84TFWGNTKK53 pKa = 10.16RR54 pKa = 11.84AQRR57 pKa = 11.84EE58 pKa = 4.0LQRR61 pKa = 11.84QIDD64 pKa = 4.27DD65 pKa = 4.34LEE67 pKa = 5.95HH68 pKa = 6.12IAWARR73 pKa = 11.84EE74 pKa = 3.78LRR76 pKa = 11.84RR77 pKa = 11.84GG78 pKa = 3.46

Molecular weight: 9.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

15676

37

1149

220.8

24.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.893 ± 0.461

0.982 ± 0.135

6.315 ± 0.248

6.673 ± 0.358

3.228 ± 0.16

8.433 ± 0.536

2.131 ± 0.216

4.835 ± 0.181

5.422 ± 0.377

8.318 ± 0.313

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.252 ± 0.103

3.54 ± 0.183

5.792 ± 0.3

3.553 ± 0.159

6.392 ± 0.395

5.314 ± 0.18

5.971 ± 0.225

6.692 ± 0.262

2.354 ± 0.14

2.909 ± 0.244
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski