Amino acid dipepetide frequency for Stx2-converting phage Stx2a_F422

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.113AlaAla: 9.113 ± 0.79
1.172AlaCys: 1.172 ± 0.315
4.69AlaAsp: 4.69 ± 0.742
8.42AlaGlu: 8.42 ± 0.795
3.624AlaPhe: 3.624 ± 0.632
7.887AlaGly: 7.887 ± 1.055
1.492AlaHis: 1.492 ± 0.308
4.583AlaIle: 4.583 ± 0.61
5.063AlaLys: 5.063 ± 0.535
7.354AlaLeu: 7.354 ± 0.739
3.038AlaMet: 3.038 ± 0.416
2.878AlaAsn: 2.878 ± 0.406
3.411AlaPro: 3.411 ± 0.462
5.489AlaGln: 5.489 ± 0.696
5.649AlaArg: 5.649 ± 0.582
5.649AlaSer: 5.649 ± 0.472
5.436AlaThr: 5.436 ± 0.741
6.342AlaVal: 6.342 ± 0.612
1.652AlaTrp: 1.652 ± 0.326
2.292AlaTyr: 2.292 ± 0.341
0.0AlaXaa: 0.0 ± 0.0
Cys
1.172CysAla: 1.172 ± 0.275
0.373CysCys: 0.373 ± 0.166
0.533CysAsp: 0.533 ± 0.173
0.853CysGlu: 0.853 ± 0.271
0.373CysPhe: 0.373 ± 0.145
0.959CysGly: 0.959 ± 0.325
0.426CysHis: 0.426 ± 0.169
0.586CysIle: 0.586 ± 0.208
0.533CysLys: 0.533 ± 0.244
0.853CysLeu: 0.853 ± 0.254
0.16CysMet: 0.16 ± 0.093
0.213CysAsn: 0.213 ± 0.11
0.48CysPro: 0.48 ± 0.176
0.426CysGln: 0.426 ± 0.145
1.119CysArg: 1.119 ± 0.312
1.119CysSer: 1.119 ± 0.334
0.693CysThr: 0.693 ± 0.181
0.906CysVal: 0.906 ± 0.277
0.16CysTrp: 0.16 ± 0.101
0.266CysTyr: 0.266 ± 0.121
0.0CysXaa: 0.0 ± 0.0
Asp
6.182AspAla: 6.182 ± 0.628
0.639AspCys: 0.639 ± 0.218
3.837AspAsp: 3.837 ± 0.374
4.423AspGlu: 4.423 ± 0.4
1.492AspPhe: 1.492 ± 0.212
4.583AspGly: 4.583 ± 0.509
0.799AspHis: 0.799 ± 0.266
3.677AspIle: 3.677 ± 0.455
4.103AspLys: 4.103 ± 0.364
4.317AspLeu: 4.317 ± 0.517
1.865AspMet: 1.865 ± 0.282
2.718AspAsn: 2.718 ± 0.421
2.398AspPro: 2.398 ± 0.425
1.492AspGln: 1.492 ± 0.367
3.304AspArg: 3.304 ± 0.348
3.144AspSer: 3.144 ± 0.373
2.611AspThr: 2.611 ± 0.366
3.997AspVal: 3.997 ± 0.443
1.172AspTrp: 1.172 ± 0.314
1.545AspTyr: 1.545 ± 0.289
0.0AspXaa: 0.0 ± 0.0
Glu
6.768GluAla: 6.768 ± 0.645
1.172GluCys: 1.172 ± 0.3
2.238GluAsp: 2.238 ± 0.332
3.997GluGlu: 3.997 ± 0.466
2.558GluPhe: 2.558 ± 0.379
3.784GluGly: 3.784 ± 0.564
1.172GluHis: 1.172 ± 0.234
4.05GluIle: 4.05 ± 0.416
4.69GluLys: 4.69 ± 0.632
5.649GluLeu: 5.649 ± 0.604
2.132GluMet: 2.132 ± 0.322
3.197GluAsn: 3.197 ± 0.461
1.812GluPro: 1.812 ± 0.438
4.37GluGln: 4.37 ± 0.505
5.649GluArg: 5.649 ± 0.638
3.73GluSer: 3.73 ± 0.405
3.784GluThr: 3.784 ± 0.671
4.157GluVal: 4.157 ± 0.582
1.172GluTrp: 1.172 ± 0.26
2.451GluTyr: 2.451 ± 0.314
0.0GluXaa: 0.0 ± 0.0
Phe
2.931PheAla: 2.931 ± 0.43
0.533PheCys: 0.533 ± 0.196
1.812PheAsp: 1.812 ± 0.314
1.172PheGlu: 1.172 ± 0.257
0.693PhePhe: 0.693 ± 0.185
2.558PheGly: 2.558 ± 0.314
0.693PheHis: 0.693 ± 0.165
1.865PheIle: 1.865 ± 0.314
1.439PheLys: 1.439 ± 0.247
1.865PheLeu: 1.865 ± 0.312
1.119PheMet: 1.119 ± 0.236
1.386PheAsn: 1.386 ± 0.308
1.172PhePro: 1.172 ± 0.231
1.013PheGln: 1.013 ± 0.235
2.078PheArg: 2.078 ± 0.357
2.718PheSer: 2.718 ± 0.424
2.451PheThr: 2.451 ± 0.446
2.451PheVal: 2.451 ± 0.368
0.799PheTrp: 0.799 ± 0.22
0.853PheTyr: 0.853 ± 0.19
0.0PheXaa: 0.0 ± 0.0
Gly
5.862GlyAla: 5.862 ± 0.969
1.013GlyCys: 1.013 ± 0.36
4.743GlyAsp: 4.743 ± 0.739
5.915GlyGlu: 5.915 ± 1.242
2.398GlyPhe: 2.398 ± 0.332
4.903GlyGly: 4.903 ± 0.667
1.066GlyHis: 1.066 ± 0.256
3.677GlyIle: 3.677 ± 0.525
4.69GlyLys: 4.69 ± 0.953
4.796GlyLeu: 4.796 ± 0.486
2.451GlyMet: 2.451 ± 0.363
2.931GlyAsn: 2.931 ± 0.363
3.784GlyPro: 3.784 ± 1.963
2.505GlyGln: 2.505 ± 0.419
3.997GlyArg: 3.997 ± 0.528
3.784GlySer: 3.784 ± 0.493
3.677GlyThr: 3.677 ± 0.478
5.755GlyVal: 5.755 ± 0.583
1.279GlyTrp: 1.279 ± 0.242
2.611GlyTyr: 2.611 ± 0.396
0.0GlyXaa: 0.0 ± 0.0
His
1.332HisAla: 1.332 ± 0.243
0.266HisCys: 0.266 ± 0.114
1.013HisAsp: 1.013 ± 0.273
0.799HisGlu: 0.799 ± 0.226
0.746HisPhe: 0.746 ± 0.218
1.705HisGly: 1.705 ± 0.383
0.426HisHis: 0.426 ± 0.146
0.746HisIle: 0.746 ± 0.211
0.906HisLys: 0.906 ± 0.266
1.599HisLeu: 1.599 ± 0.392
0.213HisMet: 0.213 ± 0.112
0.959HisAsn: 0.959 ± 0.198
0.959HisPro: 0.959 ± 0.25
0.693HisGln: 0.693 ± 0.225
0.799HisArg: 0.799 ± 0.206
0.799HisSer: 0.799 ± 0.203
1.066HisThr: 1.066 ± 0.241
0.959HisVal: 0.959 ± 0.235
0.373HisTrp: 0.373 ± 0.212
0.639HisTyr: 0.639 ± 0.186
0.0HisXaa: 0.0 ± 0.0
Ile
4.849IleAla: 4.849 ± 0.472
0.959IleCys: 0.959 ± 0.313
4.103IleAsp: 4.103 ± 0.572
3.517IleGlu: 3.517 ± 0.575
0.906IlePhe: 0.906 ± 0.21
2.931IleGly: 2.931 ± 0.486
1.013IleHis: 1.013 ± 0.254
2.292IleIle: 2.292 ± 0.348
3.411IleLys: 3.411 ± 0.482
3.411IleLeu: 3.411 ± 0.658
1.119IleMet: 1.119 ± 0.243
2.984IleAsn: 2.984 ± 0.421
2.292IlePro: 2.292 ± 0.293
1.812IleGln: 1.812 ± 0.272
4.583IleArg: 4.583 ± 0.427
3.944IleSer: 3.944 ± 0.511
3.784IleThr: 3.784 ± 0.584
2.292IleVal: 2.292 ± 0.392
0.426IleTrp: 0.426 ± 0.198
1.545IleTyr: 1.545 ± 0.349
0.0IleXaa: 0.0 ± 0.0
Lys
6.501LysAla: 6.501 ± 0.543
0.48LysCys: 0.48 ± 0.183
3.411LysAsp: 3.411 ± 0.444
3.944LysGlu: 3.944 ± 0.575
1.013LysPhe: 1.013 ± 0.225
5.436LysGly: 5.436 ± 1.043
0.906LysHis: 0.906 ± 0.265
3.464LysIle: 3.464 ± 0.477
3.73LysLys: 3.73 ± 0.583
5.116LysLeu: 5.116 ± 0.52
2.185LysMet: 2.185 ± 0.348
3.677LysAsn: 3.677 ± 0.516
2.292LysPro: 2.292 ± 0.334
3.197LysGln: 3.197 ± 0.502
3.197LysArg: 3.197 ± 0.387
2.878LysSer: 2.878 ± 0.351
3.304LysThr: 3.304 ± 0.479
3.251LysVal: 3.251 ± 0.474
0.959LysTrp: 0.959 ± 0.263
1.918LysTyr: 1.918 ± 0.377
0.0LysXaa: 0.0 ± 0.0
Leu
8.367LeuAla: 8.367 ± 0.834
1.226LeuCys: 1.226 ± 0.342
4.05LeuAsp: 4.05 ± 0.524
4.21LeuGlu: 4.21 ± 0.589
2.558LeuPhe: 2.558 ± 0.362
3.997LeuGly: 3.997 ± 0.449
1.279LeuHis: 1.279 ± 0.257
3.517LeuIle: 3.517 ± 0.512
5.009LeuLys: 5.009 ± 0.499
5.862LeuLeu: 5.862 ± 0.492
2.185LeuMet: 2.185 ± 0.368
4.157LeuAsn: 4.157 ± 0.54
3.784LeuPro: 3.784 ± 0.483
3.038LeuGln: 3.038 ± 0.672
5.276LeuArg: 5.276 ± 0.599
4.903LeuSer: 4.903 ± 0.48
5.063LeuThr: 5.063 ± 0.417
4.849LeuVal: 4.849 ± 0.427
0.746LeuTrp: 0.746 ± 0.257
2.345LeuTyr: 2.345 ± 0.341
0.0LeuXaa: 0.0 ± 0.0
Met
3.304MetAla: 3.304 ± 0.409
0.107MetCys: 0.107 ± 0.077
1.279MetAsp: 1.279 ± 0.29
1.386MetGlu: 1.386 ± 0.218
0.799MetPhe: 0.799 ± 0.213
1.439MetGly: 1.439 ± 0.337
0.373MetHis: 0.373 ± 0.122
0.799MetIle: 0.799 ± 0.214
2.398MetLys: 2.398 ± 0.381
2.292MetLeu: 2.292 ± 0.388
0.746MetMet: 0.746 ± 0.174
1.599MetAsn: 1.599 ± 0.309
1.652MetPro: 1.652 ± 0.338
1.545MetGln: 1.545 ± 0.243
1.759MetArg: 1.759 ± 0.301
1.812MetSer: 1.812 ± 0.288
2.558MetThr: 2.558 ± 0.305
1.439MetVal: 1.439 ± 0.314
0.266MetTrp: 0.266 ± 0.122
0.48MetTyr: 0.48 ± 0.164
0.0MetXaa: 0.0 ± 0.0
Asn
4.69AsnAla: 4.69 ± 0.632
0.426AsnCys: 0.426 ± 0.163
2.558AsnAsp: 2.558 ± 0.404
3.517AsnGlu: 3.517 ± 0.389
1.439AsnPhe: 1.439 ± 0.299
3.411AsnGly: 3.411 ± 0.5
1.226AsnHis: 1.226 ± 0.271
2.771AsnIle: 2.771 ± 0.494
2.078AsnLys: 2.078 ± 0.331
3.304AsnLeu: 3.304 ± 0.466
1.386AsnMet: 1.386 ± 0.273
2.025AsnAsn: 2.025 ± 0.352
1.705AsnPro: 1.705 ± 0.284
2.292AsnGln: 2.292 ± 0.387
3.091AsnArg: 3.091 ± 0.452
2.558AsnSer: 2.558 ± 0.414
2.398AsnThr: 2.398 ± 0.36
2.292AsnVal: 2.292 ± 0.37
0.426AsnTrp: 0.426 ± 0.132
1.066AsnTyr: 1.066 ± 0.287
0.0AsnXaa: 0.0 ± 0.0
Pro
3.251ProAla: 3.251 ± 0.61
0.32ProCys: 0.32 ± 0.131
4.317ProAsp: 4.317 ± 0.424
5.063ProGlu: 5.063 ± 0.788
1.279ProPhe: 1.279 ± 0.249
3.144ProGly: 3.144 ± 0.463
0.48ProHis: 0.48 ± 0.162
0.959ProIle: 0.959 ± 0.22
2.931ProLys: 2.931 ± 0.658
2.718ProLeu: 2.718 ± 0.318
0.693ProMet: 0.693 ± 0.179
1.226ProAsn: 1.226 ± 0.28
1.386ProPro: 1.386 ± 0.305
2.398ProGln: 2.398 ± 0.45
2.025ProArg: 2.025 ± 0.354
2.345ProSer: 2.345 ± 0.36
1.705ProThr: 1.705 ± 0.322
4.157ProVal: 4.157 ± 0.409
0.639ProTrp: 0.639 ± 0.189
1.599ProTyr: 1.599 ± 0.339
0.0ProXaa: 0.0 ± 0.0
Gln
4.849GlnAla: 4.849 ± 0.619
0.639GlnCys: 0.639 ± 0.197
2.451GlnAsp: 2.451 ± 0.335
3.091GlnGlu: 3.091 ± 0.438
1.759GlnPhe: 1.759 ± 0.291
3.197GlnGly: 3.197 ± 0.668
0.959GlnHis: 0.959 ± 0.232
2.078GlnIle: 2.078 ± 0.377
3.251GlnLys: 3.251 ± 0.478
3.411GlnLeu: 3.411 ± 0.357
1.119GlnMet: 1.119 ± 0.29
1.918GlnAsn: 1.918 ± 0.32
1.865GlnPro: 1.865 ± 0.38
3.944GlnGln: 3.944 ± 0.652
3.73GlnArg: 3.73 ± 0.577
2.451GlnSer: 2.451 ± 0.34
2.132GlnThr: 2.132 ± 0.37
2.345GlnVal: 2.345 ± 0.432
0.746GlnTrp: 0.746 ± 0.226
1.439GlnTyr: 1.439 ± 0.275
0.0GlnXaa: 0.0 ± 0.0
Arg
4.849ArgAla: 4.849 ± 0.409
0.32ArgCys: 0.32 ± 0.133
4.263ArgAsp: 4.263 ± 0.565
5.596ArgGlu: 5.596 ± 0.65
2.238ArgPhe: 2.238 ± 0.353
4.849ArgGly: 4.849 ± 0.857
1.545ArgHis: 1.545 ± 0.272
3.837ArgIle: 3.837 ± 0.541
4.476ArgLys: 4.476 ± 0.445
5.489ArgLeu: 5.489 ± 0.442
1.759ArgMet: 1.759 ± 0.261
3.304ArgAsn: 3.304 ± 0.436
2.132ArgPro: 2.132 ± 0.357
3.251ArgGln: 3.251 ± 0.431
5.596ArgArg: 5.596 ± 0.699
3.57ArgSer: 3.57 ± 0.419
3.464ArgThr: 3.464 ± 0.571
3.837ArgVal: 3.837 ± 0.466
1.172ArgTrp: 1.172 ± 0.237
2.025ArgTyr: 2.025 ± 0.408
0.0ArgXaa: 0.0 ± 0.0
Ser
5.702SerAla: 5.702 ± 0.518
0.533SerCys: 0.533 ± 0.19
3.677SerAsp: 3.677 ± 0.414
4.157SerGlu: 4.157 ± 0.465
1.545SerPhe: 1.545 ± 0.277
5.329SerGly: 5.329 ± 0.618
0.639SerHis: 0.639 ± 0.148
2.611SerIle: 2.611 ± 0.395
3.091SerLys: 3.091 ± 0.559
5.382SerLeu: 5.382 ± 0.645
1.599SerMet: 1.599 ± 0.324
2.398SerAsn: 2.398 ± 0.302
3.144SerPro: 3.144 ± 0.433
3.091SerGln: 3.091 ± 0.338
3.837SerArg: 3.837 ± 0.599
3.304SerSer: 3.304 ± 0.578
3.464SerThr: 3.464 ± 0.455
3.73SerVal: 3.73 ± 0.436
0.906SerTrp: 0.906 ± 0.223
1.599SerTyr: 1.599 ± 0.295
0.0SerXaa: 0.0 ± 0.0
Thr
5.276ThrAla: 5.276 ± 0.434
0.48ThrCys: 0.48 ± 0.174
3.091ThrAsp: 3.091 ± 0.426
3.411ThrGlu: 3.411 ± 0.403
2.078ThrPhe: 2.078 ± 0.44
5.702ThrGly: 5.702 ± 0.76
0.959ThrHis: 0.959 ± 0.258
4.05ThrIle: 4.05 ± 0.48
3.091ThrLys: 3.091 ± 0.399
4.69ThrLeu: 4.69 ± 0.522
0.799ThrMet: 0.799 ± 0.187
1.918ThrAsn: 1.918 ± 0.304
3.624ThrPro: 3.624 ± 0.364
1.599ThrGln: 1.599 ± 0.292
2.611ThrArg: 2.611 ± 0.351
3.837ThrSer: 3.837 ± 0.533
3.091ThrThr: 3.091 ± 0.42
4.317ThrVal: 4.317 ± 0.444
0.853ThrTrp: 0.853 ± 0.223
1.119ThrTyr: 1.119 ± 0.281
0.0ThrXaa: 0.0 ± 0.0
Val
6.448ValAla: 6.448 ± 0.605
1.066ValCys: 1.066 ± 0.262
3.944ValAsp: 3.944 ± 0.382
3.304ValGlu: 3.304 ± 0.337
2.025ValPhe: 2.025 ± 0.346
3.144ValGly: 3.144 ± 0.442
0.533ValHis: 0.533 ± 0.148
3.89ValIle: 3.89 ± 0.496
3.411ValLys: 3.411 ± 0.372
5.596ValLeu: 5.596 ± 0.589
1.652ValMet: 1.652 ± 0.295
3.197ValAsn: 3.197 ± 0.396
2.771ValPro: 2.771 ± 0.38
2.238ValGln: 2.238 ± 0.342
5.329ValArg: 5.329 ± 0.888
4.636ValSer: 4.636 ± 0.525
3.89ValThr: 3.89 ± 0.479
3.944ValVal: 3.944 ± 0.487
0.853ValTrp: 0.853 ± 0.223
2.185ValTyr: 2.185 ± 0.35
0.0ValXaa: 0.0 ± 0.0
Trp
1.013TrpAla: 1.013 ± 0.321
0.213TrpCys: 0.213 ± 0.094
0.426TrpAsp: 0.426 ± 0.148
0.48TrpGlu: 0.48 ± 0.146
0.693TrpPhe: 0.693 ± 0.128
0.639TrpGly: 0.639 ± 0.17
0.32TrpHis: 0.32 ± 0.139
0.906TrpIle: 0.906 ± 0.204
1.279TrpLys: 1.279 ± 0.219
1.545TrpLeu: 1.545 ± 0.395
0.959TrpMet: 0.959 ± 0.261
0.693TrpAsn: 0.693 ± 0.202
0.639TrpPro: 0.639 ± 0.222
1.279TrpGln: 1.279 ± 0.21
1.279TrpArg: 1.279 ± 0.276
0.906TrpSer: 0.906 ± 0.212
0.48TrpThr: 0.48 ± 0.165
1.172TrpVal: 1.172 ± 0.296
0.32TrpTrp: 0.32 ± 0.175
0.373TrpTyr: 0.373 ± 0.121
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.771TyrAla: 2.771 ± 0.399
0.266TyrCys: 0.266 ± 0.127
1.865TyrAsp: 1.865 ± 0.344
1.279TyrGlu: 1.279 ± 0.324
1.386TyrPhe: 1.386 ± 0.233
2.238TyrGly: 2.238 ± 0.374
0.693TyrHis: 0.693 ± 0.257
1.812TyrIle: 1.812 ± 0.326
1.279TyrLys: 1.279 ± 0.27
1.226TyrLeu: 1.226 ± 0.241
0.799TyrMet: 0.799 ± 0.225
1.279TyrAsn: 1.279 ± 0.233
1.332TyrPro: 1.332 ± 0.212
1.705TyrGln: 1.705 ± 0.245
2.665TyrArg: 2.665 ± 0.461
1.652TyrSer: 1.652 ± 0.289
1.545TyrThr: 1.545 ± 0.428
1.865TyrVal: 1.865 ± 0.28
0.693TyrTrp: 0.693 ± 0.182
1.226TyrTyr: 1.226 ± 0.21
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 91 proteins (18766 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski